LOCUS       Exported     2780 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..2780
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      793..840
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     2668..2684
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      2685..2716
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             1..0
                     /note="TrmD: tRNA (guanine(37)-N(1))-methyltransferase"
                     /translation="MKFSIITLFPKIINSYIEESIIKRAINKQAIQIEVIDLRDFSTLSHNQVDDYQYGGGSGMVLMIEPLIRAIESVKTTNSIVLLTTPQGKTLNQSIVKKYANNDEHIIIICGHYEGYDERILDYIDDEISIGDYVITGGELASLILVDSISRIIPNVIKQESHENESFENNLLDHPVYTKPYEFRNNKVPDVLLSGHHQNIKKWREEQQVIKTLKKRPDLIDVNKLNKHQLEIYKKMKGEQ"
                     /label="MMSYN1_0364"
     primer_bind     complement(845..861)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(928..1516)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(1687..2547)
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
                     /label="bla"
     promoter        complement(2548..2652)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
     CDS             complement(1..0)
                     /note="TrmD: tRNA (guanine(37)-N(1))-methyltransferase"
                     /translation="MKFSIITLFPKIINSYIEESIIKRAINKQAIQIEVIDLRDFSTLSHNQVDDYQYGGGSGMVLMIEPLIRAIESVKTTNSIVLLTTPQGKTLNQSIVKKYANNDEHIIIICGHYEGYDERILDYIDDEISIGDYVITGGELASLILVDSISRIIPNVIKQESHENESFENNLLDHPVYTKPYEFRNNKVPDVLLSGHHQNIKKWREEQQVIKTLKKRPDLIDVNKLNKHQLEIYKKMKGEQ"
                     /label="MMSYN1_0364"
ORIGIN
        1 ATGAAATTCT CCATCATTAC TTTATTCCCC AAGATCATTA ACTCTTACAT CGAAGAATCC
       61 ATTATTAAAC GCGCTATCAA TAAGCAAGCA ATTCAAATTG AGGTCATTGA TCTTCGTGAT
      121 TTCTCCACAT TGTCGCACAA TCAAGTGGAT GATTACCAGT ATGGCGGAGG GTCAGGCATG
      181 GTACTGATGA TCGAACCGCT GATTCGTGCC ATCGAGTCGG TCAAGACGAC CAATTCCATT
      241 GTACTGTTGA CTACGCCACA AGGCAAGACA TTAAATCAAT CCATCGTAAA GAAGTATGCC
      301 AACAATGACG AGCACATCAT TATCATTTGT GGTCATTATG AGGGGTACGA TGAGCGTATC
      361 TTAGATTACA TTGACGACGA AATTAGCATT GGGGATTATG TCATCACGGG AGGGGAGTTG
      421 GCGTCCCTTA TCTTAGTGGA TTCCATCAGC CGTATCATTC CTAATGTTAT CAAACAAGAG
      481 AGTCATGAGA ACGAGAGCTT CGAGAATAAT CTGTTGGACC ACCCAGTTTA TACCAAGCCC
      541 TATGAATTTC GTAACAATAA GGTGCCCGAC GTCCTGCTGA GCGGCCACCA TCAAAATATT
      601 AAAAAGTGGC GCGAGGAACA ACAGGTCATT AAGACTTTGA AGAAGCGCCC CGACCTTATC
      661 GATGTCAATA AACTGAATAA ACACCAATTG GAAATCTATA AAAAGATGAA AGGCGAACAG
      721 TGAAGAGCTT AGAGACCGGT ACTAGTTCCT GCAGGGCTCG AGGTGTCAAT CGTCGGAGCC
      781 GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG
      841 CATGGTCATA GCTGTTTCCT GAGAGCTTGG CAGGTGATGA CACACATTAA CAAATTTCGT
      901 GAGGAGTCTC CAGAAGAATG CCATTAATTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA
      961 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC
     1021 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA
     1081 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA
     1141 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA
     1201 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA
     1261 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG
     1321 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG
     1381 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG
     1441 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG
     1501 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA
     1561 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT
     1621 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AAACTTGGTC
     1681 TGACAGTTAC CAATGCTTAA TCAGTGAGGC ACCTATCTCA GCGATCTGTC TATTTCGTTC
     1741 ATCCATAGTT GCCTGACTCC CCGTCGTGTA GATAACTACG ATACGGGAGG GCTTACCATC
     1801 TGGCCCCAGT GCTGCAATGA TACCGCGAGA ACCACGCTCA CCGGCTCCAG ATTTATCAGC
     1861 AATAAACCAG CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT CCTGCAACTT TATCCGCCTC
     1921 CATCCAGTCT ATTAATTGTT GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG TTAATAGTTT
     1981 GCGCAACGTT GTTGCCATTG CTACAGGCAT CGTGGTGTCA CGCTCGTCGT TTGGTATGGC
     2041 TTCATTCAGC TCCGGTTCCC AACGATCAAG GCGAGTTACA TGATCCCCCA TGTTGTGCAA
     2101 AAAAGCGGTT AGCTCCTTCG GTCCTCCGAT CGTTGTCAGA AGTAAGTTGG CCGCAGTGTT
     2161 ATCACTCATG GTTATGGCAG CACTGCATAA TTCTCTTACT GTCATGCCAT CCGTAAGATG
     2221 CTTTTCTGTG ACTGGTGAGT ACTCAACCAA GTCATTCTGA GAATAGTGTA TGCGGCGACC
     2281 GAGTTGCTCT TGCCCGGCGT CAATACGGGA TAATACCGCG CCACATAGCA GAACTTTAAA
     2341 AGTGCTCATC ATTGGAAAAC GTTCTTCGGG GCGAAAACTC TCAAGGATCT TACCGCTGTT
     2401 GAGATCCAGT TCGATGTAAC CCACTCGTGC ACCCAACTGA TCTTCAGCAT CTTTTACTTT
     2461 CACCAGCGTT TCTGGGTGAG CAAAAACAGG AAGGCAAAAT GCCGCAAAAA AGGGAATAAG
     2521 GGCGACACGG AAATGTTGAA TACTCATACT CTTCCTTTTT CAATATTATT GAAGCATTTA
     2581 TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT
     2641 AGGGGTTCCG CGCACCTGCA CCAGTCAGTA AAACGACGGC CAGTAGTCAA AAGCCTCCGA
     2701 CCGGAGGCTT TTGACTTGGT CAGGTGGAGT GAATTCGCGG CCGCTTCTAG AGGCTCTTCG
     2761 TAATTACGAA GAGGTCTCTA
//