LOCUS       Exported     3895 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3895
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      38..85
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     1933..1949
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      1950..1981
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             2010..3881
                     /label="MMSYN1_0371"
                     /note="ABC transporter, ATP-binding protein"
                     /translation="MKVKNNFDHFYKPMTDEEIKADRKSFNRGRKSFINVIWKHMKINKKWAIGLLITAIFSALFAALNPLLMQQLQFAVEFEKTHQNFSNSWGLSWKVILAIWIVILVITAILTYIANLFGNELGKKIEISLRNELTRKLITTDIHYYSNKKTGEILTKVVSDTQIIGMQASVIPNIIFTAFFTMVFTLITLFITTSLYIGLFFISLFLMFGILFGLSFLPMRKLVFNLRKIITDINGDVTDRINTIKLIKANGTEEYEKTRFVQIHDVYYKKYKQISYFQSVMISILFFAINTVQILMTLIALWLYKNDITTLKTILGPMLICAGMLIGPIMQLLRAIIGMVQASTSAQRIDEITDATQLINNHSLDKKGIRIHKIEGNLVFKNVNFSYPDKPENVILPNFNLVLEKGKSYAFVGQTGAGKSTISKLLLRFYDPTSGEVLINDNINIKDVFLPSYLNHIGYVEQDPSVLLGTVFDNLRYVKPSATDEEIILACKKAELHDLVTTWPEQYNTILGERGFILSGGQKQRLVIARMFLKNPDILILDEATSALDNVVEKEIQAKLEELMQGRTSITIAHRLSTIRNVDQIIVLAPKKGIIQIGTFKELVKKPGEFKDLYEAGFSKYDA"
     primer_bind     complement(90..106)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(173..761)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(952..1812)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(1813..1917)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC
       61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT
      121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA
      181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC
      241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG
      301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC
      361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG
      421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC
      481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA
      541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG
      601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA
      661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG
      721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA
      781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA
      841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA
      901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG
      961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG
     1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC
     1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC
     1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA
     1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC
     1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG
     1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC
     1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT
     1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG
     1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC
     1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG
     1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT
     1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG
     1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG
     1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT
     1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC
     1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC
     1981 TTGGTTCAGG TGGAGTGGGA GGGTCTCAAA TGAAAGTAAA GAATAATTTT GATCATTTTT
     2041 ACAAGCCAAT GACGGACGAA GAGATCAAGG CCGACCGTAA GTCTTTTAAC CGTGGCCGTA
     2101 AGTCCTTTAT CAATGTTATC TGGAAGCACA TGAAGATTAA CAAAAAGTGG GCCATTGGAC
     2161 TGTTAATCAC GGCGATCTTC TCCGCGTTAT TTGCAGCTTT GAACCCCTTG CTGATGCAAC
     2221 AACTGCAGTT TGCAGTCGAA TTTGAAAAAA CCCACCAAAA CTTCTCGAAC AGTTGGGGGC
     2281 TTTCTTGGAA AGTTATCTTG GCAATCTGGA TTGTGATTTT AGTCATTACC GCAATCTTAA
     2341 CGTATATTGC GAACTTATTC GGAAATGAAT TGGGGAAGAA AATCGAAATT AGCTTGCGCA
     2401 ATGAGTTGAC ACGTAAATTG ATTACTACGG ATATCCACTA TTATTCTAAC AAAAAGACAG
     2461 GAGAAATCTT GACCAAAGTT GTATCAGATA CACAGATCAT CGGAATGCAG GCCTCGGTTA
     2521 TCCCTAATAT TATTTTCACG GCCTTCTTTA CAATGGTGTT CACGCTTATT ACACTTTTCA
     2581 TCACAACCTC GTTGTACATC GGGTTATTCT TTATCAGTTT ATTTTTAATG TTTGGAATTC
     2641 TTTTTGGCCT GAGTTTCCTT CCGATGCGTA AATTGGTGTT CAATCTTCGC AAGATCATTA
     2701 CAGACATTAA TGGGGACGTA ACAGATCGCA TCAACACGAT CAAGTTGATC AAGGCAAACG
     2761 GGACTGAAGA GTACGAGAAA ACACGTTTTG TACAAATTCA CGATGTTTAC TATAAAAAGT
     2821 ACAAACAAAT CAGCTATTTT CAATCTGTTA TGATCAGCAT CCTGTTTTTT GCGATTAACA
     2881 CTGTACAGAT TCTGATGACA TTGATTGCCT TGTGGTTATA CAAAAATGAC ATCACTACGT
     2941 TAAAAACCAT TCTGGGTCCA ATGTTAATCT GCGCTGGAAT GCTTATCGGC CCAATTATGC
     3001 AGTTGTTGCG CGCTATCATT GGCATGGTTC AGGCATCAAC CTCTGCCCAA CGTATCGACG
     3061 AAATCACGGA CGCCACTCAA TTAATTAATA ATCATTCGTT GGATAAGAAG GGGATCCGTA
     3121 TTCACAAGAT CGAAGGTAAC TTAGTTTTTA AGAACGTAAA TTTCTCATAT CCCGACAAAC
     3181 CAGAGAACGT CATCCTGCCT AATTTCAATT TGGTATTAGA AAAGGGAAAG AGTTATGCGT
     3241 TTGTTGGGCA AACCGGAGCC GGAAAGTCTA CTATCTCGAA ACTTTTATTG CGCTTTTATG
     3301 ACCCTACTAG TGGAGAAGTC TTGATCAACG ATAATATCAA TATCAAAGAT GTTTTCCTGC
     3361 CCTCTTATTT AAACCACATC GGGTATGTTG AACAAGATCC CTCGGTACTT TTAGGGACAG
     3421 TATTCGACAA TCTGCGCTAT GTCAAGCCAT CGGCGACGGA CGAAGAAATT ATTCTGGCTT
     3481 GCAAGAAAGC CGAATTGCAT GACTTAGTAA CGACTTGGCC AGAACAGTAC AATACCATTC
     3541 TTGGGGAACG TGGTTTCATT TTGTCAGGCG GACAAAAACA GCGCCTGGTG ATCGCGCGTA
     3601 TGTTTCTGAA GAACCCAGAC ATCTTGATTT TAGATGAAGC TACATCAGCA CTTGACAACG
     3661 TGGTCGAAAA AGAAATCCAA GCCAAATTAG AGGAATTGAT GCAGGGGCGT ACCTCGATCA
     3721 CTATCGCTCA TCGTTTATCA ACCATTCGCA ACGTTGACCA AATTATTGTC TTGGCTCCAA
     3781 AGAAAGGAAT TATTCAGATC GGGACTTTTA AGGAATTAGT GAAAAAGCCC GGCGAGTTCA
     3841 AAGATCTGTA TGAGGCCGGT TTCAGCAAAT ATGATGCCTG AAGAGCTTAG AGACC
//