LOCUS       Exported     3665 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3665
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1678..1725
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     3553..3569
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      3570..3601
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
                     /label="tonB terminator"
     CDS             1..0
                     /note="hypothetical protein"
                     /translation="MPVQESIYWVYFHDMVKKIKTDRFKKVDELLKKKINEIFEITHYGLFQYQILKDKPLINIDDSSISEICKYITNNYLRFFEYLNYNNSKTSVYSSKLTKNELEEISFIIENISIRYIADNLILTNNNNYNSDFLTLLLIELSKMHRFDTNFLARNNDKIVYHSLVYPLFLTMLVIDITNEAQMFNNIKKIYTKQNILNALKSGRPLSSNELNYFKSHIDILEYDEEWNTFLLNFKQENWTSFSVEKKYKLVFQLAKYTALFLKDRIKSVWALSDGEEIFDSFYNYINLFLINKTSNQTSTIYLTNKIDPLNKNYDDSDRFLLPFLIKDYNPIQIGHHISSLKDYSKFVCDKDRIIDFLDAVLLSTNYINLIDILKVDSNYLADFLIQRKKLALVDTLNLYKLNDHNIYKKQYNSINLEDLKFNQDVLKEIIKKDFRIEVLKTNNQFVNMLKIISLILALVPSTARRYNYSWELIVKYFIITFGPYKRKKALYDKKTINEITYKISKLLSNFKHVKNKDDYSRTLLIIHKLENFKN"
                     /label="MMSYN1_0373"
     primer_bind     complement(1730..1746)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1813..2401)
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
                     /label="ori"
     CDS             complement(2572..3432)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(3433..3537)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
     CDS             complement(1..0)
                     /label="MMSYN1_0373"
                     /note="hypothetical protein"
                     /translation="MPVQESIYWVYFHDMVKKIKTDRFKKVDELLKKKINEIFEITHYGLFQYQILKDKPLINIDDSSISEICKYITNNYLRFFEYLNYNNSKTSVYSSKLTKNELEEISFIIENISIRYIADNLILTNNNNYNSDFLTLLLIELSKMHRFDTNFLARNNDKIVYHSLVYPLFLTMLVIDITNEAQMFNNIKKIYTKQNILNALKSGRPLSSNELNYFKSHIDILEYDEEWNTFLLNFKQENWTSFSVEKKYKLVFQLAKYTALFLKDRIKSVWALSDGEEIFDSFYNYINLFLINKTSNQTSTIYLTNKIDPLNKNYDDSDRFLLPFLIKDYNPIQIGHHISSLKDYSKFVCDKDRIIDFLDAVLLSTNYINLIDILKVDSNYLADFLIQRKKLALVDTLNLYKLNDHNIYKKQYNSINLEDLKFNQDVLKEIIKKDFRIEVLKTNNQFVNMLKIISLILALVPSTARRYNYSWELIVKYFIITFGPYKRKKALYDKKTINEITYKISKLLSNFKHVKNKDDYSRTLLIIHKLENFKN"
ORIGIN
        1 ATGCCTGTGC AGGAATCAAT TTACTGGGTA TACTTTCATG ATATGGTAAA GAAGATCAAA
       61 ACCGATCGTT TCAAGAAAGT AGACGAGTTA TTGAAAAAGA AGATCAACGA AATTTTCGAG
      121 ATTACACACT ATGGGTTATT TCAGTATCAA ATTCTGAAGG ACAAGCCACT TATTAATATT
      181 GATGATTCGT CTATTAGTGA AATCTGCAAA TATATCACAA ACAACTATCT TCGCTTTTTT
      241 GAGTATTTGA ACTATAACAA TTCTAAGACC TCAGTGTATT CATCGAAGTT GACTAAAAAC
      301 GAATTAGAAG AAATTTCATT TATTATTGAG AATATTTCGA TCCGCTATAT CGCGGATAAT
      361 CTTATTCTTA CGAATAACAA CAACTACAAC TCAGACTTTC TTACATTATT ATTGATCGAG
      421 CTTTCGAAGA TGCATCGTTT CGACACCAAC TTTTTAGCAC GCAACAACGA CAAGATTGTA
      481 TATCATAGTT TAGTGTACCC GTTGTTCCTT ACTATGTTAG TCATCGACAT TACGAATGAG
      541 GCACAGATGT TCAATAACAT TAAGAAAATC TACACTAAAC AAAATATCTT GAACGCGTTA
      601 AAGTCGGGCC GCCCATTAAG CAGCAACGAG CTTAACTACT TCAAATCTCA CATCGATATT
      661 TTGGAATATG ACGAAGAGTG GAACACATTT CTTCTTAACT TTAAGCAGGA AAACTGGACT
      721 TCCTTTTCTG TCGAGAAAAA GTATAAGCTT GTATTCCAAC TTGCTAAGTA TACGGCTTTG
      781 TTTTTGAAAG ATCGTATCAA GTCGGTCTGG GCACTTTCCG ACGGCGAAGA AATTTTCGAC
      841 TCCTTCTACA ACTATATTAA TTTATTCCTG ATTAATAAAA CGTCGAACCA GACTTCTACC
      901 ATTTACTTGA CGAACAAGAT CGACCCCTTA AATAAGAACT ATGACGACTC CGACCGCTTC
      961 CTGTTGCCAT TCCTGATCAA GGACTACAAT CCTATCCAAA TCGGTCATCA CATTTCCTCG
     1021 TTAAAGGACT ATAGTAAATT TGTATGTGAC AAAGATCGCA TTATCGACTT TCTGGATGCA
     1081 GTGCTGCTGT CTACCAATTA TATCAATTTG ATCGACATTC TTAAAGTGGA TTCGAACTAC
     1141 CTGGCTGATT TTTTGATTCA GCGTAAGAAA TTAGCTCTGG TGGACACCTT GAATTTATAC
     1201 AAGCTTAACG ATCACAATAT CTATAAGAAG CAATACAACT CAATTAACCT GGAGGATCTT
     1261 AAATTCAATC AGGATGTCTT GAAGGAGATT ATTAAAAAGG ATTTCCGCAT CGAAGTTCTT
     1321 AAAACAAATA ACCAATTCGT AAATATGTTA AAAATCATCA GTTTAATTCT GGCATTGGTG
     1381 CCGAGCACGG CCCGCCGCTA CAATTATAGC TGGGAATTGA TTGTCAAGTA CTTCATCATC
     1441 ACATTCGGCC CATATAAGCG TAAAAAGGCA TTATACGATA AAAAGACCAT TAATGAGATT
     1501 ACATATAAAA TCAGTAAGCT GTTATCCAAT TTTAAACACG TGAAAAACAA GGACGATTAC
     1561 AGTCGCACAC TTTTGATCAT CCACAAATTA GAAAACTTCA AGAACTGAAG AGCTTAGAGA
     1621 CCGGTACTAG TTCCTGCAGG GCTCGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA
     1681 GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT
     1741 TTCCTGAGAG CTTGGCAGGT GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA
     1801 GAATGCCATT AATTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC
     1861 AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG
     1921 CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT
     1981 CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA
     2041 GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC
     2101 CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC
     2161 AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT
     2221 GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT
     2281 GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC
     2341 TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA
     2401 AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA
     2461 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA
     2521 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG
     2581 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG
     2641 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC
     2701 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC
     2761 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA
     2821 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC
     2881 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG
     2941 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC
     3001 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT
     3061 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG
     3121 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC
     3181 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG
     3241 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT
     3301 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG
     3361 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG
     3421 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT
     3481 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC
     3541 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC
     3601 TTGGTCAGGT GGAGTGAATT CGCGGCCGCT TCTAGAGGCT CTTCGTAATT ACGAAGAGGT
     3661 CTCTA
//