LOCUS       Exported     2795 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..2795
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
                     /label="T7 terminator"
     terminator      808..855
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     2683..2699
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      2700..2731
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
                     /label="tonB terminator"
     CDS             1..0
                     /translation="MQTNLKQYLDYLVEFIQQTVKKAKCDGVVVGISGGIDSAVVANLAKLAFPNNYLTVWMPIYSSQLDYDCANELIKTNQLKNIEVNLEASFDAFKNSFSNLDEKPNLLAISNAKARLRMTTLYTIAQTKKYLVLGTDNLDEWHIGYFTKYGDGGVDVVPIIHLLKSEVKKAAQILNVPEIIINRKPTAGLWEGQTDEGEIGFSYDLIDSYLLKQNNDPELKKRIDYLHKISKHKRSLAIKPKKIIR"
                     /label="MMSYN1_0378"
                     /note="NAD+ synthetase"
     primer_bind     complement(860..876)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(943..1531)
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
                     /label="ori"
     CDS             complement(1702..2562)
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
                     /label="bla"
     promoter        complement(2563..2667)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
     CDS             complement(1..0)
                     /label="MMSYN1_0378"
                     /note="NAD+ synthetase"
                     /translation="MQTNLKQYLDYLVEFIQQTVKKAKCDGVVVGISGGIDSAVVANLAKLAFPNNYLTVWMPIYSSQLDYDCANELIKTNQLKNIEVNLEASFDAFKNSFSNLDEKPNLLAISNAKARLRMTTLYTIAQTKKYLVLGTDNLDEWHIGYFTKYGDGGVDVVPIIHLLKSEVKKAAQILNVPEIIINRKPTAGLWEGQTDEGEIGFSYDLIDSYLLKQNNDPELKKRIDYLHKISKHKRSLAIKPKKIIR"
ORIGIN
        1 ATGCAGACCA ACCTGAAACA GTATCTGGAT TATCTGGTGG AATTTATTCA GCAGACCGTG
       61 AAGAAAGCGA AATGTGATGG CGTGGTGGTG GGCATTAGCG GCGGCATTGA TAGCGCGGTG
      121 GTGGCGAACC TGGCGAAACT GGCGTTTCCG AACAACTATC TGACCGTGTG GATGCCGATT
      181 TATAGCAGCC AGCTGGATTA TGATTGCGCG AACGAACTGA TTAAAACCAA CCAGCTGAAA
      241 AACATTGAAG TGAACCTGGA AGCGAGCTTT GATGCGTTTA AAAACAGCTT TAGCAACCTG
      301 GATGAAAAAC CGAATCTGCT GGCGATTAGC AACGCGAAAG CGCGCCTGCG CATGACCACC
      361 CTGTATACCA TTGCGCAGAC CAAGAAATAT CTGGTGCTGG GCACCGATAA CCTGGATGAA
      421 TGGCATATTG GCTATTTTAC CAAATATGGA GATGGCGGCG TGGATGTGGT GCCGATTATT
      481 CATCTGCTGA AAAGCGAAGT GAAGAAAGCG GCGCAGATTC TGAACGTGCC GGAAATTATT
      541 ATTAACCGCA AACCGACCGC GGGCCTGTGG GAAGGCCAGA CCGATGAAGG CGAAATTGGC
      601 TTTAGCTATG ATCTGATTGA TAGCTATCTG CTGAAACAGA ACAACGATCC GGAACTGAAG
      661 AAACGCATTG ATTATCTGCA TAAAATTAGC AAACATAAAC GCAGCCTGGC GATTAAACCG
      721 AAGAAAATTA TTCGCTGAAG AGCTTAGAGA CCGGTACTAG TTCCTGCAGG GCTCGAGGTG
      781 TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC
      841 TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT GATGACACAC
      901 ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA GGCTCCGCCC
      961 CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT
     1021 ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT
     1081 GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG
     1141 CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA
     1201 CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA
     1261 CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC
     1321 GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG
     1381 AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG
     1441 TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA
     1501 GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC
     1561 TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG
     1621 GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA
     1681 TGAGTAAACT TGGTCTGACA GTTACCAATG CTTAATCAGT GAGGCACCTA TCTCAGCGAT
     1741 CTGTCTATTT CGTTCATCCA TAGTTGCCTG ACTCCCCGTC GTGTAGATAA CTACGATACG
     1801 GGAGGGCTTA CCATCTGGCC CCAGTGCTGC AATGATACCG CGAGAACCAC GCTCACCGGC
     1861 TCCAGATTTA TCAGCAATAA ACCAGCCAGC CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC
     1921 AACTTTATCC GCCTCCATCC AGTCTATTAA TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC
     1981 GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC CATTGCTACA GGCATCGTGG TGTCACGCTC
     2041 GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG TTCCCAACGA TCAAGGCGAG TTACATGATC
     2101 CCCCATGTTG TGCAAAAAAG CGGTTAGCTC CTTCGGTCCT CCGATCGTTG TCAGAAGTAA
     2161 GTTGGCCGCA GTGTTATCAC TCATGGTTAT GGCAGCACTG CATAATTCTC TTACTGTCAT
     2221 GCCATCCGTA AGATGCTTTT CTGTGACTGG TGAGTACTCA ACCAAGTCAT TCTGAGAATA
     2281 GTGTATGCGG CGACCGAGTT GCTCTTGCCC GGCGTCAATA CGGGATAATA CCGCGCCACA
     2341 TAGCAGAACT TTAAAAGTGC TCATCATTGG AAAACGTTCT TCGGGGCGAA AACTCTCAAG
     2401 GATCTTACCG CTGTTGAGAT CCAGTTCGAT GTAACCCACT CGTGCACCCA ACTGATCTTC
     2461 AGCATCTTTT ACTTTCACCA GCGTTTCTGG GTGAGCAAAA ACAGGAAGGC AAAATGCCGC
     2521 AAAAAAGGGA ATAAGGGCGA CACGGAAATG TTGAATACTC ATACTCTTCC TTTTTCAATA
     2581 TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA
     2641 GAAAAATAAA CAAATAGGGG TTCCGCGCAC CTGCACCAGT CAGTAAAACG ACGGCCAGTA
     2701 GTCAAAAGCC TCCGACCGGA GGCTTTTGAC TTGGTCAGGT GGAGTGAATT CGCGGCCGCT
     2761 TCTAGAGGCT CTTCGTAATT ACGAAGAGGT CTCTA
//