LOCUS Exported 2795 bp DNA circular SYN 24-JUL-2020 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2001) AUTHORS Keoni Gandall TITLE Direct Submission JOURNAL Exported from Poly. FEATURES Location/Qualifiers terminator 1..2795 /note="transcription terminator for bacteriophage T7 RNA polymerase" /label="T7 terminator" terminator 808..855 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" primer_bind 2683..2699 /label="M13 for" /note="common sequencing primer, one of multiple similar variants" terminator 2700..2731 /note="bidirectional E. coli tonB-P14 transcription terminator" /label="tonB terminator" CDS 1..0 /translation="MQTNLKQYLDYLVEFIQQTVKKAKCDGVVVGISGGIDSAVVANLAKLAFPNNYLTVWMPIYSSQLDYDCANELIKTNQLKNIEVNLEASFDAFKNSFSNLDEKPNLLAISNAKARLRMTTLYTIAQTKKYLVLGTDNLDEWHIGYFTKYGDGGVDVVPIIHLLKSEVKKAAQILNVPEIIINRKPTAGLWEGQTDEGEIGFSYDLIDSYLLKQNNDPELKKRIDYLHKISKHKRSLAIKPKKIIR" /label="MMSYN1_0378" /note="NAD+ synthetase" primer_bind complement(860..876) /label="M13 rev" /note="common sequencing primer, one of multiple similar variants" rep_origin complement(943..1531) /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(1702..2562) /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*" /label="bla" promoter complement(2563..2667) /label="bla promoter" /note="ampicillin resistance gene promoter" CDS complement(1..0) /label="MMSYN1_0378" /note="NAD+ synthetase" /translation="MQTNLKQYLDYLVEFIQQTVKKAKCDGVVVGISGGIDSAVVANLAKLAFPNNYLTVWMPIYSSQLDYDCANELIKTNQLKNIEVNLEASFDAFKNSFSNLDEKPNLLAISNAKARLRMTTLYTIAQTKKYLVLGTDNLDEWHIGYFTKYGDGGVDVVPIIHLLKSEVKKAAQILNVPEIIINRKPTAGLWEGQTDEGEIGFSYDLIDSYLLKQNNDPELKKRIDYLHKISKHKRSLAIKPKKIIR" ORIGIN 1 ATGCAGACCA ACCTGAAACA GTATCTGGAT TATCTGGTGG AATTTATTCA GCAGACCGTG 61 AAGAAAGCGA AATGTGATGG CGTGGTGGTG GGCATTAGCG GCGGCATTGA TAGCGCGGTG 121 GTGGCGAACC TGGCGAAACT GGCGTTTCCG AACAACTATC TGACCGTGTG GATGCCGATT 181 TATAGCAGCC AGCTGGATTA TGATTGCGCG AACGAACTGA TTAAAACCAA CCAGCTGAAA 241 AACATTGAAG TGAACCTGGA AGCGAGCTTT GATGCGTTTA AAAACAGCTT TAGCAACCTG 301 GATGAAAAAC CGAATCTGCT GGCGATTAGC AACGCGAAAG CGCGCCTGCG CATGACCACC 361 CTGTATACCA TTGCGCAGAC CAAGAAATAT CTGGTGCTGG GCACCGATAA CCTGGATGAA 421 TGGCATATTG GCTATTTTAC CAAATATGGA GATGGCGGCG TGGATGTGGT GCCGATTATT 481 CATCTGCTGA AAAGCGAAGT GAAGAAAGCG GCGCAGATTC TGAACGTGCC GGAAATTATT 541 ATTAACCGCA AACCGACCGC GGGCCTGTGG GAAGGCCAGA CCGATGAAGG CGAAATTGGC 601 TTTAGCTATG ATCTGATTGA TAGCTATCTG CTGAAACAGA ACAACGATCC GGAACTGAAG 661 AAACGCATTG ATTATCTGCA TAAAATTAGC AAACATAAAC GCAGCCTGGC GATTAAACCG 721 AAGAAAATTA TTCGCTGAAG AGCTTAGAGA CCGGTACTAG TTCCTGCAGG GCTCGAGGTG 781 TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC 841 TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT GATGACACAC 901 ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA GGCTCCGCCC 961 CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT 1021 ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT 1081 GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG 1141 CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA 1201 CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA 1261 CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC 1321 GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG 1381 AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG 1441 TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA 1501 GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC 1561 TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG 1621 GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA 1681 TGAGTAAACT TGGTCTGACA GTTACCAATG CTTAATCAGT GAGGCACCTA TCTCAGCGAT 1741 CTGTCTATTT CGTTCATCCA TAGTTGCCTG ACTCCCCGTC GTGTAGATAA CTACGATACG 1801 GGAGGGCTTA CCATCTGGCC CCAGTGCTGC AATGATACCG CGAGAACCAC GCTCACCGGC 1861 TCCAGATTTA TCAGCAATAA ACCAGCCAGC CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC 1921 AACTTTATCC GCCTCCATCC AGTCTATTAA TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC 1981 GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC CATTGCTACA GGCATCGTGG TGTCACGCTC 2041 GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG TTCCCAACGA TCAAGGCGAG TTACATGATC 2101 CCCCATGTTG TGCAAAAAAG CGGTTAGCTC CTTCGGTCCT CCGATCGTTG TCAGAAGTAA 2161 GTTGGCCGCA GTGTTATCAC TCATGGTTAT GGCAGCACTG CATAATTCTC TTACTGTCAT 2221 GCCATCCGTA AGATGCTTTT CTGTGACTGG TGAGTACTCA ACCAAGTCAT TCTGAGAATA 2281 GTGTATGCGG CGACCGAGTT GCTCTTGCCC GGCGTCAATA CGGGATAATA CCGCGCCACA 2341 TAGCAGAACT TTAAAAGTGC TCATCATTGG AAAACGTTCT TCGGGGCGAA AACTCTCAAG 2401 GATCTTACCG CTGTTGAGAT CCAGTTCGAT GTAACCCACT CGTGCACCCA ACTGATCTTC 2461 AGCATCTTTT ACTTTCACCA GCGTTTCTGG GTGAGCAAAA ACAGGAAGGC AAAATGCCGC 2521 AAAAAAGGGA ATAAGGGCGA CACGGAAATG TTGAATACTC ATACTCTTCC TTTTTCAATA 2581 TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA 2641 GAAAAATAAA CAAATAGGGG TTCCGCGCAC CTGCACCAGT CAGTAAAACG ACGGCCAGTA 2701 GTCAAAAGCC TCCGACCGGA GGCTTTTGAC TTGGTCAGGT GGAGTGAATT CGCGGCCGCT 2761 TCTAGAGGCT CTTCGTAATT ACGAAGAGGT CTCTA //