LOCUS Exported 2717 bp DNA circular SYN 24-JUL-2020 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2001) AUTHORS Keoni Gandall TITLE Direct Submission JOURNAL Exported from Poly. FEATURES Location/Qualifiers terminator 1..2717 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" terminator 730..777 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" primer_bind 2605..2621 /note="common sequencing primer, one of multiple similar variants" /label="M13 for" terminator 2622..2653 /label="tonB terminator" /note="bidirectional E. coli tonB-P14 transcription terminator" CDS 1..0 /label="MMSYN1_0381" /note="Catalyzes the irreversible cleavage of the glycosidic bond in both 5'-methylthioadenosine (MTA) and S-adenosylhomocysteine (SAH/AdoHcy) to adenine and the corresponding thioribose, 5'-methylthioribose and S-ribosylhomocysteine, respectively." /translation="MKLIISAMYEELAYTLKKTNAQLIIDNDILKLYQYQNILLAISKIGLVNASCCLSYILNHYQIDQILNIGTCCSLNQEYKQNDIVIVNKAYYFSVDVTGFSYSYGQIPKLPKYFLANNFLNLSLDYKICNIASGDVFINKSEHLTQFINKINDKIDIVDMEACSLFHVAYLYKRPISSIKVISDVMFVSDSNMLQFDQFINKASKKIYQILNDLYFKID" primer_bind complement(782..798) /label="M13 rev" /note="common sequencing primer, one of multiple similar variants" rep_origin complement(865..1453) /label="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(1624..2484) /label="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*" promoter complement(2485..2589) /label="bla promoter" /note="ampicillin resistance gene promoter" CDS complement(1..0) /label="MMSYN1_0381" /note="Catalyzes the irreversible cleavage of the glycosidic bond in both 5'-methylthioadenosine (MTA) and S-adenosylhomocysteine (SAH/AdoHcy) to adenine and the corresponding thioribose, 5'-methylthioribose and S-ribosylhomocysteine, respectively." /translation="MKLIISAMYEELAYTLKKTNAQLIIDNDILKLYQYQNILLAISKIGLVNASCCLSYILNHYQIDQILNIGTCCSLNQEYKQNDIVIVNKAYYFSVDVTGFSYSYGQIPKLPKYFLANNFLNLSLDYKICNIASGDVFINKSEHLTQFINKINDKIDIVDMEACSLFHVAYLYKRPISSIKVISDVMFVSDSNMLQFDQFINKASKKIYQILNDLYFKID" ORIGIN 1 ATGAAACTTA TCATTTCTGC AATGTATGAG GAACTGGCCT ATACTCTGAA GAAAACCAAT 61 GCACAGTTAA TTATCGACAA TGACATCCTT AAGCTGTATC AATATCAAAA TATCCTGCTG 121 GCAATTAGCA AGATTGGATT AGTCAATGCT TCCTGCTGTT TAAGCTACAT CCTTAACCAT 181 TACCAAATTG ACCAAATCCT TAATATCGGG ACTTGTTGCT CTCTTAACCA GGAGTACAAG 241 CAAAACGATA TCGTAATCGT TAATAAGGCC TATTACTTTA GCGTTGACGT AACGGGGTTC 301 TCTTACAGTT ACGGCCAGAT CCCGAAATTG CCCAAGTATT TTTTGGCAAA TAACTTCTTA 361 AATCTTTCTT TGGACTACAA AATTTGCAAT ATTGCATCTG GTGATGTATT CATTAATAAA 421 TCTGAACACC TGACGCAGTT CATTAACAAG ATTAATGACA AAATCGACAT CGTGGATATG 481 GAAGCATGTT CATTATTTCA TGTGGCGTAC CTGTATAAGC GCCCAATTTC ATCTATTAAA 541 GTAATCTCTG ACGTTATGTT CGTTTCGGAT TCTAACATGC TGCAGTTTGA CCAGTTCATT 601 AACAAAGCTA GTAAGAAAAT TTATCAAATT TTAAACGATT TGTACTTTAA GATCGATTGA 661 AGAGCTTAGA GACCGGTACT AGTTCCTGCA GGGCTCGAGG TGTCAATCGT CGGAGCCGCT 721 GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCAT 781 GGTCATAGCT GTTTCCTGAG AGCTTGGCAG GTGATGACAC ACATTAACAA ATTTCGTGAG 841 GAGTCTCCAG AAGAATGCCA TTAATTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA 901 AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT 961 TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT 1021 GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT 1081 CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC 1141 CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT 1201 ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC 1261 TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT 1321 CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA 1381 ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA 1441 AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA 1501 AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT 1561 TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAA CTTGGTCTGA 1621 CAGTTACCAA TGCTTAATCA GTGAGGCACC TATCTCAGCG ATCTGTCTAT TTCGTTCATC 1681 CATAGTTGCC TGACTCCCCG TCGTGTAGAT AACTACGATA CGGGAGGGCT TACCATCTGG 1741 CCCCAGTGCT GCAATGATAC CGCGAGAACC ACGCTCACCG GCTCCAGATT TATCAGCAAT 1801 AAACCAGCCA GCCGGAAGGG CCGAGCGCAG AAGTGGTCCT GCAACTTTAT CCGCCTCCAT 1861 CCAGTCTATT AATTGTTGCC GGGAAGCTAG AGTAAGTAGT TCGCCAGTTA ATAGTTTGCG 1921 CAACGTTGTT GCCATTGCTA CAGGCATCGT GGTGTCACGC TCGTCGTTTG GTATGGCTTC 1981 ATTCAGCTCC GGTTCCCAAC GATCAAGGCG AGTTACATGA TCCCCCATGT TGTGCAAAAA 2041 AGCGGTTAGC TCCTTCGGTC CTCCGATCGT TGTCAGAAGT AAGTTGGCCG CAGTGTTATC 2101 ACTCATGGTT ATGGCAGCAC TGCATAATTC TCTTACTGTC ATGCCATCCG TAAGATGCTT 2161 TTCTGTGACT GGTGAGTACT CAACCAAGTC ATTCTGAGAA TAGTGTATGC GGCGACCGAG 2221 TTGCTCTTGC CCGGCGTCAA TACGGGATAA TACCGCGCCA CATAGCAGAA CTTTAAAAGT 2281 GCTCATCATT GGAAAACGTT CTTCGGGGCG AAAACTCTCA AGGATCTTAC CGCTGTTGAG 2341 ATCCAGTTCG ATGTAACCCA CTCGTGCACC CAACTGATCT TCAGCATCTT TTACTTTCAC 2401 CAGCGTTTCT GGGTGAGCAA AAACAGGAAG GCAAAATGCC GCAAAAAAGG GAATAAGGGC 2461 GACACGGAAA TGTTGAATAC TCATACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA 2521 GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG 2581 GGTTCCGCGC ACCTGCACCA GTCAGTAAAA CGACGGCCAG TAGTCAAAAG CCTCCGACCG 2641 GAGGCTTTTG ACTTGGTCAG GTGGAGTGAA TTCGCGGCCG CTTCTAGAGG CTCTTCGTAA 2701 TTACGAAGAG GTCTCTA //