LOCUS       Exported     2717 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..2717
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      730..777
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     2605..2621
                     /note="common sequencing primer, one of multiple similar variants"
                     /label="M13 for"
     terminator      2622..2653
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             1..0
                     /label="MMSYN1_0381"
                     /note="Catalyzes the irreversible cleavage of the glycosidic bond in both 5'-methylthioadenosine (MTA) and S-adenosylhomocysteine (SAH/AdoHcy) to adenine and the corresponding thioribose, 5'-methylthioribose and S-ribosylhomocysteine, respectively."
                     /translation="MKLIISAMYEELAYTLKKTNAQLIIDNDILKLYQYQNILLAISKIGLVNASCCLSYILNHYQIDQILNIGTCCSLNQEYKQNDIVIVNKAYYFSVDVTGFSYSYGQIPKLPKYFLANNFLNLSLDYKICNIASGDVFINKSEHLTQFINKINDKIDIVDMEACSLFHVAYLYKRPISSIKVISDVMFVSDSNMLQFDQFINKASKKIYQILNDLYFKID"
     primer_bind     complement(782..798)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(865..1453)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(1624..2484)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(2485..2589)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
     CDS             complement(1..0)
                     /label="MMSYN1_0381"
                     /note="Catalyzes the irreversible cleavage of the glycosidic bond in both 5'-methylthioadenosine (MTA) and S-adenosylhomocysteine (SAH/AdoHcy) to adenine and the corresponding thioribose, 5'-methylthioribose and S-ribosylhomocysteine, respectively."
                     /translation="MKLIISAMYEELAYTLKKTNAQLIIDNDILKLYQYQNILLAISKIGLVNASCCLSYILNHYQIDQILNIGTCCSLNQEYKQNDIVIVNKAYYFSVDVTGFSYSYGQIPKLPKYFLANNFLNLSLDYKICNIASGDVFINKSEHLTQFINKINDKIDIVDMEACSLFHVAYLYKRPISSIKVISDVMFVSDSNMLQFDQFINKASKKIYQILNDLYFKID"
ORIGIN
        1 ATGAAACTTA TCATTTCTGC AATGTATGAG GAACTGGCCT ATACTCTGAA GAAAACCAAT
       61 GCACAGTTAA TTATCGACAA TGACATCCTT AAGCTGTATC AATATCAAAA TATCCTGCTG
      121 GCAATTAGCA AGATTGGATT AGTCAATGCT TCCTGCTGTT TAAGCTACAT CCTTAACCAT
      181 TACCAAATTG ACCAAATCCT TAATATCGGG ACTTGTTGCT CTCTTAACCA GGAGTACAAG
      241 CAAAACGATA TCGTAATCGT TAATAAGGCC TATTACTTTA GCGTTGACGT AACGGGGTTC
      301 TCTTACAGTT ACGGCCAGAT CCCGAAATTG CCCAAGTATT TTTTGGCAAA TAACTTCTTA
      361 AATCTTTCTT TGGACTACAA AATTTGCAAT ATTGCATCTG GTGATGTATT CATTAATAAA
      421 TCTGAACACC TGACGCAGTT CATTAACAAG ATTAATGACA AAATCGACAT CGTGGATATG
      481 GAAGCATGTT CATTATTTCA TGTGGCGTAC CTGTATAAGC GCCCAATTTC ATCTATTAAA
      541 GTAATCTCTG ACGTTATGTT CGTTTCGGAT TCTAACATGC TGCAGTTTGA CCAGTTCATT
      601 AACAAAGCTA GTAAGAAAAT TTATCAAATT TTAAACGATT TGTACTTTAA GATCGATTGA
      661 AGAGCTTAGA GACCGGTACT AGTTCCTGCA GGGCTCGAGG TGTCAATCGT CGGAGCCGCT
      721 GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCAT
      781 GGTCATAGCT GTTTCCTGAG AGCTTGGCAG GTGATGACAC ACATTAACAA ATTTCGTGAG
      841 GAGTCTCCAG AAGAATGCCA TTAATTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA
      901 AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT
      961 TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT
     1021 GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT
     1081 CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC
     1141 CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT
     1201 ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC
     1261 TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT
     1321 CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA
     1381 ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA
     1441 AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA
     1501 AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT
     1561 TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAA CTTGGTCTGA
     1621 CAGTTACCAA TGCTTAATCA GTGAGGCACC TATCTCAGCG ATCTGTCTAT TTCGTTCATC
     1681 CATAGTTGCC TGACTCCCCG TCGTGTAGAT AACTACGATA CGGGAGGGCT TACCATCTGG
     1741 CCCCAGTGCT GCAATGATAC CGCGAGAACC ACGCTCACCG GCTCCAGATT TATCAGCAAT
     1801 AAACCAGCCA GCCGGAAGGG CCGAGCGCAG AAGTGGTCCT GCAACTTTAT CCGCCTCCAT
     1861 CCAGTCTATT AATTGTTGCC GGGAAGCTAG AGTAAGTAGT TCGCCAGTTA ATAGTTTGCG
     1921 CAACGTTGTT GCCATTGCTA CAGGCATCGT GGTGTCACGC TCGTCGTTTG GTATGGCTTC
     1981 ATTCAGCTCC GGTTCCCAAC GATCAAGGCG AGTTACATGA TCCCCCATGT TGTGCAAAAA
     2041 AGCGGTTAGC TCCTTCGGTC CTCCGATCGT TGTCAGAAGT AAGTTGGCCG CAGTGTTATC
     2101 ACTCATGGTT ATGGCAGCAC TGCATAATTC TCTTACTGTC ATGCCATCCG TAAGATGCTT
     2161 TTCTGTGACT GGTGAGTACT CAACCAAGTC ATTCTGAGAA TAGTGTATGC GGCGACCGAG
     2221 TTGCTCTTGC CCGGCGTCAA TACGGGATAA TACCGCGCCA CATAGCAGAA CTTTAAAAGT
     2281 GCTCATCATT GGAAAACGTT CTTCGGGGCG AAAACTCTCA AGGATCTTAC CGCTGTTGAG
     2341 ATCCAGTTCG ATGTAACCCA CTCGTGCACC CAACTGATCT TCAGCATCTT TTACTTTCAC
     2401 CAGCGTTTCT GGGTGAGCAA AAACAGGAAG GCAAAATGCC GCAAAAAAGG GAATAAGGGC
     2461 GACACGGAAA TGTTGAATAC TCATACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA
     2521 GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG
     2581 GGTTCCGCGC ACCTGCACCA GTCAGTAAAA CGACGGCCAG TAGTCAAAAG CCTCCGACCG
     2641 GAGGCTTTTG ACTTGGTCAG GTGGAGTGAA TTCGCGGCCG CTTCTAGAGG CTCTTCGTAA
     2701 TTACGAAGAG GTCTCTA
//