LOCUS       Exported     4168 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..4168
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      38..85
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
                     /label="T7 terminator"
     primer_bind     1933..1949
                     /note="common sequencing primer, one of multiple similar variants"
                     /label="M13 for"
     terminator      1950..1981
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
                     /label="tonB terminator"
     CDS             2010..4154
                     /label="MMSYN1_0389"
                     /note=""
                     /translation="MNSIFKINISKEIFKIANLKCIKIAWILQNINNFKKAVEWNKTKKYFFNIDHDLESEDDFSSDSTSINLFEEYTNTDLKTEQERAEFLKKWESFFNSDDGFRLDEFKGDAIEDGLEFGKKVIEYFDLKQIKEYPNKLTKDFNDTANIYDAVNQTKELLKNHQDQYVYLYEPAFEFDNFNLKVKCDVLKLNGDNHVEIIEAKATSKVKKEHFWDLVYQVYVLERNGFIVDNIAIARLNKNYLRDYDSNVDFDLKTSIEEFASQYKDINFDQAKKIVDNIDDLDLGFKNIDEIDDLDLNKLIEIDYFTYGQAKTRNTLIEDYKNLINVVDIDELFLKIAYMLRLDENQIIEIFKNDSCYLHYDKKGKNWIKWTREISDYKACQHVLDWFDEKAPNFWHFGGAKQTQKAFLIRHLHSPYFKDYNSLLDSEITNLLNDQYDKFINYKYNRIFKISKLDDQIKSDPSLMIDNNYFYILKQVMNKYKTLPIYMYDFETVKFAVPKYSKVNPYYQIPFQYSIDIIHDKNYDYNNPDSMIHYDFLANDYQDPRKEFIINFLKDIFSNQKGVYVAYNDAFEKSVLKRIAFLFPKLAIPILYIVNNTIDLMDFFKGVKQDSSIDANFRPWFLIANKNFYGSYSIKKTQPALDSTFTYKNLTINNGSKASETFRRFLEQRIERTVWDNLIRKDMIKYCNRDTLAMVVILKKVDEIIKIWEAKHGK"
     primer_bind     complement(90..106)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(173..761)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(952..1812)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(1813..1917)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC
       61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT
      121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA
      181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC
      241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG
      301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC
      361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG
      421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC
      481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA
      541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG
      601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA
      661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG
      721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA
      781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA
      841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA
      901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG
      961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG
     1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC
     1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC
     1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA
     1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC
     1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG
     1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC
     1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT
     1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG
     1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC
     1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG
     1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT
     1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG
     1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG
     1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT
     1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC
     1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC
     1981 TTGGTTCAGG TGGAGTGGGA GGGTCTCAAA TGAACTCCAT CTTTAAGATC AACATCAGTA
     2041 AAGAAATTTT CAAGATCGCG AACCTTAAGT GCATCAAAAT TGCGTGGATC TTACAAAACA
     2101 TTAATAACTT CAAGAAAGCC GTGGAGTGGA ACAAGACGAA AAAGTACTTT TTCAACATCG
     2161 ACCATGATCT GGAGTCAGAG GATGATTTCA GCTCAGATTC AACCAGTATT AATTTATTCG
     2221 AAGAGTATAC CAATACTGAT TTAAAGACGG AACAAGAACG CGCAGAATTT CTTAAGAAAT
     2281 GGGAATCTTT TTTCAATTCA GATGACGGTT TCCGTTTGGA CGAATTCAAA GGTGATGCGA
     2341 TTGAGGACGG CTTAGAATTT GGGAAGAAGG TGATTGAGTA TTTCGATCTG AAGCAGATTA
     2401 AAGAATACCC TAATAAGCTG ACGAAGGATT TTAATGATAC TGCGAACATT TACGATGCCG
     2461 TGAATCAGAC AAAAGAACTT TTAAAGAATC ATCAGGATCA GTACGTATAC TTATACGAGC
     2521 CGGCTTTTGA GTTCGATAAT TTTAACTTGA AAGTCAAGTG TGATGTCCTT AAGCTGAACG
     2581 GAGACAATCA CGTCGAGATC ATTGAGGCCA AAGCTACCTC GAAAGTGAAA AAGGAACACT
     2641 TCTGGGATTT AGTCTACCAA GTTTACGTTC TTGAACGCAA CGGCTTTATT GTGGACAATA
     2701 TTGCGATCGC TCGCTTAAAT AAAAACTACT TACGCGATTA TGACTCGAAT GTTGATTTCG
     2761 ATTTAAAAAC GTCAATTGAA GAGTTTGCTT CTCAGTATAA GGACATTAAT TTTGATCAGG
     2821 CGAAGAAAAT TGTGGACAAC ATTGACGACC TTGATCTTGG GTTCAAGAAC ATTGATGAGA
     2881 TCGACGACTT GGATCTTAAT AAGTTGATCG AAATTGATTA CTTCACGTAT GGTCAGGCCA
     2941 AAACGCGCAA TACCTTGATT GAGGACTACA AAAATCTTAT TAACGTGGTC GACATCGATG
     3001 AGTTATTTCT GAAAATCGCT TACATGTTAC GTTTGGACGA GAATCAAATC ATTGAAATCT
     3061 TCAAGAATGA CTCATGTTAT CTGCACTACG ACAAGAAAGG TAAGAATTGG ATTAAATGGA
     3121 CTCGCGAAAT CTCCGATTAC AAGGCGTGTC AACATGTGTT AGACTGGTTT GATGAGAAGG
     3181 CCCCCAACTT TTGGCACTTT GGTGGTGCGA AGCAAACGCA AAAGGCGTTC TTAATTCGTC
     3241 ACTTACACAG TCCGTATTTC AAAGACTATA ACAGTTTATT AGATTCTGAA ATCACAAACC
     3301 TGTTAAATGA TCAGTACGAT AAATTTATTA ACTATAAATA CAATCGCATT TTCAAAATTT
     3361 CGAAATTGGA CGACCAAATT AAGAGTGATC CGTCCTTAAT GATCGATAAT AATTATTTTT
     3421 ATATTTTGAA ACAGGTTATG AATAAGTATA AGACCTTGCC CATCTACATG TACGATTTTG
     3481 AAACGGTAAA ATTTGCCGTC CCTAAGTACT CAAAAGTTAA TCCATATTAC CAGATCCCTT
     3541 TCCAATATTC GATTGACATT ATCCATGATA AAAACTATGA CTACAACAAC CCTGATTCTA
     3601 TGATCCACTA TGACTTTCTT GCTAACGACT ATCAAGACCC CCGCAAAGAA TTCATCATTA
     3661 ACTTTTTAAA GGATATTTTC AGTAACCAAA AGGGTGTATA TGTCGCCTAC AATGATGCGT
     3721 TTGAAAAAAG TGTGTTGAAA CGCATTGCAT TCTTGTTCCC CAAGTTGGCC ATTCCCATCT
     3781 TATATATCGT CAATAATACA ATCGATCTGA TGGATTTTTT CAAAGGGGTC AAGCAGGACT
     3841 CTAGTATTGA TGCAAACTTC CGCCCATGGT TCTTAATCGC AAATAAGAAT TTCTACGGTT
     3901 CTTACTCGAT TAAAAAGACG CAACCAGCAC TGGACTCCAC GTTCACATAT AAAAATTTGA
     3961 CAATCAATAA CGGCAGCAAA GCAAGCGAAA CCTTCCGTCG CTTCCTTGAA CAACGCATCG
     4021 AGCGTACTGT ATGGGATAAC TTGATCCGTA AGGATATGAT TAAATATTGT AACCGCGATA
     4081 CTCTTGCTAT GGTCGTTATC CTTAAGAAGG TCGATGAAAT TATCAAAATT TGGGAAGCTA
     4141 AGCACGGGAA GTGAAGAGCT TAGAGACC
//