LOCUS       Exported     3011 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3011
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1024..1071
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     2899..2915
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      2916..2947
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             1..0
                     /label="MMSYN1_0390"
                     /note="Attaches a formyl group to the free amino group of methionyl-tRNA(fMet). The formyl group appears to play a dual role in the initiator identity of N-formylmethionyl-tRNA by promoting its recognition by IF2 and preventing the misappropriation of this tRNA by the elongation apparatus."
                     /translation="MENKIKIVFCGTPKIGADVLKALIEMNQVEVVLVISQPDKPIGRKKQIVYTPVKKLALENNLKVVQPNKIGEIYDDLAKLEFDFLITCAFGQFIPTKILKLAKIDSINFHGSLLPKLRGGAPIQYAIKNGDKKTGITIMQMVKQMDAGDYYVQESIDILDSDDSGSLFEKMGQLAYSMCKKYLVDIYNHKFELIKQNEDEVTFCKNISSEEEKINWNNTSLDIFNLIRSLSPSPISYTTINNQRYKIKSSKVIDLDNQNKNVMPGTIIDINKQGIVVKTLDKALLILEIQKEGKKMILASNYYLNKLSDLKINDKFD"
     primer_bind     complement(1076..1092)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1159..1747)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(1918..2778)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(2779..2883)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
     CDS             complement(1..0)
                     /label="MMSYN1_0390"
                     /note="Attaches a formyl group to the free amino group of methionyl-tRNA(fMet). The formyl group appears to play a dual role in the initiator identity of N-formylmethionyl-tRNA by promoting its recognition by IF2 and preventing the misappropriation of this tRNA by the elongation apparatus."
                     /translation="MENKIKIVFCGTPKIGADVLKALIEMNQVEVVLVISQPDKPIGRKKQIVYTPVKKLALENNLKVVQPNKIGEIYDDLAKLEFDFLITCAFGQFIPTKILKLAKIDSINFHGSLLPKLRGGAPIQYAIKNGDKKTGITIMQMVKQMDAGDYYVQESIDILDSDDSGSLFEKMGQLAYSMCKKYLVDIYNHKFELIKQNEDEVTFCKNISSEEEKINWNNTSLDIFNLIRSLSPSPISYTTINNQRYKIKSSKVIDLDNQNKNVMPGTIIDINKQGIVVKTLDKALLILEIQKEGKKMILASNYYLNKLSDLKINDKFD"
ORIGIN
        1 ATGGAAAATA AAATCAAGAT TGTTTTCTGC GGTACGCCCA AGATCGGCGC TGACGTCTTG
       61 AAAGCATTGA TTGAGATGAA CCAAGTCGAA GTAGTGCTTG TAATTAGTCA ACCCGACAAA
      121 CCTATTGGAC GCAAAAAGCA GATTGTTTAT ACGCCTGTCA AAAAGCTTGC CTTAGAAAAC
      181 AACTTAAAGG TCGTTCAGCC AAACAAGATT GGCGAAATTT ACGATGACCT GGCAAAGCTG
      241 GAGTTTGATT TCCTTATCAC GTGCGCGTTC GGCCAGTTTA TTCCAACCAA AATCCTTAAA
      301 CTGGCCAAGA TTGACTCGAT TAACTTTCAC GGGTCTTTAT TACCAAAACT TCGCGGAGGG
      361 GCACCGATCC AGTACGCAAT CAAGAATGGC GATAAAAAGA CTGGGATTAC GATTATGCAA
      421 ATGGTGAAAC AGATGGATGC GGGCGACTAT TATGTGCAGG AGAGTATCGA CATCTTAGAT
      481 AGTGATGATA GCGGTTCTCT TTTCGAAAAA ATGGGACAAC TTGCATACAG CATGTGCAAA
      541 AAGTACTTAG TCGACATTTA CAATCATAAA TTCGAGCTTA TCAAACAAAA TGAGGACGAG
      601 GTCACGTTCT GCAAAAACAT CTCATCAGAA GAGGAAAAAA TCAACTGGAA TAATACCTCG
      661 CTTGACATCT TTAACTTAAT CCGTAGTTTG AGTCCCTCTC CTATCTCATA CACCACGATC
      721 AACAACCAGC GTTATAAGAT TAAGAGCTCA AAAGTGATTG ATCTGGACAA TCAGAATAAG
      781 AATGTAATGC CCGGTACAAT TATCGACATT AATAAGCAGG GAATCGTTGT CAAGACCCTT
      841 GATAAGGCTC TGTTAATTCT TGAAATTCAG AAGGAGGGCA AGAAGATGAT CTTAGCCTCA
      901 AACTACTATC TTAACAAACT GAGTGACTTG AAAATCAACG ATAAATTCGA TTGAAGAGCT
      961 TAGAGACCGG TACTAGTTCC TGCAGGGCTC GAGGTGTCAA TCGTCGGAGC CGCTGAGCAA
     1021 TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCATGGTCAT
     1081 AGCTGTTTCC TGAGAGCTTG GCAGGTGATG ACACACATTA ACAAATTTCG TGAGGAGTCT
     1141 CCAGAAGAAT GCCATTAATT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG
     1201 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC
     1261 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC
     1321 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC
     1381 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG
     1441 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC
     1501 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA
     1561 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC
     1621 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC
     1681 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG
     1741 ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC
     1801 ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA
     1861 TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAAACTTGGT CTGACAGTTA
     1921 CCAATGCTTA ATCAGTGAGG CACCTATCTC AGCGATCTGT CTATTTCGTT CATCCATAGT
     1981 TGCCTGACTC CCCGTCGTGT AGATAACTAC GATACGGGAG GGCTTACCAT CTGGCCCCAG
     2041 TGCTGCAATG ATACCGCGAG AACCACGCTC ACCGGCTCCA GATTTATCAG CAATAAACCA
     2101 GCCAGCCGGA AGGGCCGAGC GCAGAAGTGG TCCTGCAACT TTATCCGCCT CCATCCAGTC
     2161 TATTAATTGT TGCCGGGAAG CTAGAGTAAG TAGTTCGCCA GTTAATAGTT TGCGCAACGT
     2221 TGTTGCCATT GCTACAGGCA TCGTGGTGTC ACGCTCGTCG TTTGGTATGG CTTCATTCAG
     2281 CTCCGGTTCC CAACGATCAA GGCGAGTTAC ATGATCCCCC ATGTTGTGCA AAAAAGCGGT
     2341 TAGCTCCTTC GGTCCTCCGA TCGTTGTCAG AAGTAAGTTG GCCGCAGTGT TATCACTCAT
     2401 GGTTATGGCA GCACTGCATA ATTCTCTTAC TGTCATGCCA TCCGTAAGAT GCTTTTCTGT
     2461 GACTGGTGAG TACTCAACCA AGTCATTCTG AGAATAGTGT ATGCGGCGAC CGAGTTGCTC
     2521 TTGCCCGGCG TCAATACGGG ATAATACCGC GCCACATAGC AGAACTTTAA AAGTGCTCAT
     2581 CATTGGAAAA CGTTCTTCGG GGCGAAAACT CTCAAGGATC TTACCGCTGT TGAGATCCAG
     2641 TTCGATGTAA CCCACTCGTG CACCCAACTG ATCTTCAGCA TCTTTTACTT TCACCAGCGT
     2701 TTCTGGGTGA GCAAAAACAG GAAGGCAAAA TGCCGCAAAA AAGGGAATAA GGGCGACACG
     2761 GAAATGTTGA ATACTCATAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA
     2821 TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC
     2881 GCGCACCTGC ACCAGTCAGT AAAACGACGG CCAGTAGTCA AAAGCCTCCG ACCGGAGGCT
     2941 TTTGACTTGG TCAGGTGGAG TGAATTCGCG GCCGCTTCTA GAGGCTCTTC GTAATTACGA
     3001 AGAGGTCTCT A
//