LOCUS Exported 2360 bp DNA circular SYN 24-JUL-2020 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2001) AUTHORS Keoni Gandall TITLE Direct Submission JOURNAL Exported from Poly. FEATURES Location/Qualifiers terminator 1..2360 /note="transcription terminator for bacteriophage T7 RNA polymerase" /label="T7 terminator" terminator 373..420 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" primer_bind 2248..2264 /label="M13 for" /note="common sequencing primer, one of multiple similar variants" terminator 2265..2296 /note="bidirectional E. coli tonB-P14 transcription terminator" /label="tonB terminator" CDS 1..0 /label="MMSYN1_0392" /note="" /translation="MYIDIEKNSKGNLKIESKVINRLVENVILSMTKISDPKNVSSSIYVLDENQLHILATIKIGDEKLQDLNINEDKIFKAIDKTINQTISMKPKNINISYIR" primer_bind complement(425..441) /label="M13 rev" /note="common sequencing primer, one of multiple similar variants" rep_origin complement(508..1096) /label="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(1267..2127) /label="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*" promoter complement(2128..2232) /label="bla promoter" /note="ampicillin resistance gene promoter" CDS complement(1..0) /label="MMSYN1_0392" /note="" /translation="MYIDIEKNSKGNLKIESKVINRLVENVILSMTKISDPKNVSSSIYVLDENQLHILATIKIGDEKLQDLNINEDKIFKAIDKTINQTISMKPKNINISYIR" ORIGIN 1 ATGTACATCG ACATTGAAAA GAATTCTAAA GGTAATCTGA AGATTGAAAG CAAAGTTATC 61 AATCGTCTTG TCGAAAACGT GATCTTATCC ATGACGAAGA TCTCTGACCC AAAAAATGTA 121 TCCTCGTCTA TCTACGTATT GGACGAAAAC CAACTGCATA TCTTAGCCAC CATCAAAATC 181 GGGGATGAAA AGTTACAGGA TTTGAACATC AATGAGGACA AAATTTTTAA GGCGATCGAT 241 AAGACTATCA ACCAGACGAT CAGTATGAAA CCTAAGAACA TCAACATCTC CTACATTCGC 301 TGAAGAGCTT AGAGACCGGT ACTAGTTCCT GCAGGGCTCG AGGTGTCAAT CGTCGGAGCC 361 GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG 421 CATGGTCATA GCTGTTTCCT GAGAGCTTGG CAGGTGATGA CACACATTAA CAAATTTCGT 481 GAGGAGTCTC CAGAAGAATG CCATTAATTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA 541 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC 601 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA 661 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA 721 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA 781 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA 841 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG 901 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG 961 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG 1021 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG 1081 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA 1141 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT 1201 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AAACTTGGTC 1261 TGACAGTTAC CAATGCTTAA TCAGTGAGGC ACCTATCTCA GCGATCTGTC TATTTCGTTC 1321 ATCCATAGTT GCCTGACTCC CCGTCGTGTA GATAACTACG ATACGGGAGG GCTTACCATC 1381 TGGCCCCAGT GCTGCAATGA TACCGCGAGA ACCACGCTCA CCGGCTCCAG ATTTATCAGC 1441 AATAAACCAG CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT CCTGCAACTT TATCCGCCTC 1501 CATCCAGTCT ATTAATTGTT GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG TTAATAGTTT 1561 GCGCAACGTT GTTGCCATTG CTACAGGCAT CGTGGTGTCA CGCTCGTCGT TTGGTATGGC 1621 TTCATTCAGC TCCGGTTCCC AACGATCAAG GCGAGTTACA TGATCCCCCA TGTTGTGCAA 1681 AAAAGCGGTT AGCTCCTTCG GTCCTCCGAT CGTTGTCAGA AGTAAGTTGG CCGCAGTGTT 1741 ATCACTCATG GTTATGGCAG CACTGCATAA TTCTCTTACT GTCATGCCAT CCGTAAGATG 1801 CTTTTCTGTG ACTGGTGAGT ACTCAACCAA GTCATTCTGA GAATAGTGTA TGCGGCGACC 1861 GAGTTGCTCT TGCCCGGCGT CAATACGGGA TAATACCGCG CCACATAGCA GAACTTTAAA 1921 AGTGCTCATC ATTGGAAAAC GTTCTTCGGG GCGAAAACTC TCAAGGATCT TACCGCTGTT 1981 GAGATCCAGT TCGATGTAAC CCACTCGTGC ACCCAACTGA TCTTCAGCAT CTTTTACTTT 2041 CACCAGCGTT TCTGGGTGAG CAAAAACAGG AAGGCAAAAT GCCGCAAAAA AGGGAATAAG 2101 GGCGACACGG AAATGTTGAA TACTCATACT CTTCCTTTTT CAATATTATT GAAGCATTTA 2161 TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT 2221 AGGGGTTCCG CGCACCTGCA CCAGTCAGTA AAACGACGGC CAGTAGTCAA AAGCCTCCGA 2281 CCGGAGGCTT TTGACTTGGT CAGGTGGAGT GAATTCGCGG CCGCTTCTAG AGGCTCTTCG 2341 TAATTACGAA GAGGTCTCTA //