LOCUS       Exported     4384 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..4384
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      38..85
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     1933..1949
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      1950..1981
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             2010..4370
                     /label="MMSYN1_0394"
                     /note="lon: endopeptidase La"
                     /translation="MKTIKLPVVVTRGIFILPSTSKTIEFGRVKSKNALNASADLYNNQIVVVSQESPLEEEPNLEHLFYLGTVADLSVKKVWKDGTISVELNYNQKIKIDEFVEEDNIIYAIGSVFEDKLPKTDAQKTKIKEALEELQEKHSFNTSELLLAFNENDFNKLNSLIYQIIDKMPLVSLNTKLLLIQSTSILEKLDLLKELIINRPKSTVKLNNNLNNNSTVDSEINKKLKDKMDKQQKEYYLREKMRIIKEELDDENSDASQLDKYKKRLEEEPFPESVKEKILSSIKRIETMQPGSAEVNVERNYVDWMMSIPWWEQSEDIDDLKYAQEILEKHHFGLKKVKERIIEYLAVKQKTKSLKGPIITFVGPPGVGKTSLARSIAEALGKKFVKVSLGGVKDESEIRGHRKTYVGSMPGRIIQALKRAKVKNPLFLLDEIDKMASDNRGDPASAMLEVLDPEQNKEFSDHYIEEPYDLSTVMFIATANYIENIPEALYDRMEIINLSSYTEIEKMHIAKDYLTKKILEEDQLTEDELRFTDEAYDEIIKYYTREAGVRQLERHLATIARKFIVKLLNGKITNLVVTREVVVQYLGKHIFEHTSKEEESQVGVVTGLAYTQFGGDILPIEVSTYNGKGNLTLTGKLGEVMKESATIALTYVKANHEKFGISKDKFDDIDIHIHVPEGAVPKDGPSAGITLTTALISALSKQPVSKDFGMTGEITLRGNVLPIGGLREKSISAARSGLKHILIPSKNVKDIEDVPQEVQDVLKITPVSKYEDVYEIIFKNNNQQTI"
     primer_bind     complement(90..106)
                     /note="common sequencing primer, one of multiple similar variants"
                     /label="M13 rev"
     rep_origin      complement(173..761)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(952..1812)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(1813..1917)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC
       61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT
      121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA
      181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC
      241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG
      301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC
      361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG
      421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC
      481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA
      541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG
      601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA
      661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG
      721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA
      781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA
      841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA
      901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG
      961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG
     1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC
     1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC
     1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA
     1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC
     1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG
     1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC
     1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT
     1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG
     1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC
     1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG
     1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT
     1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG
     1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG
     1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT
     1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC
     1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC
     1981 TTGGTTCAGG TGGAGTGGGA GGGTCTCAAA TGAAAACAAT TAAGCTTCCG GTTGTTGTAA
     2041 CTCGCGGCAT TTTCATCCTT CCCTCGACAT CTAAGACAAT CGAATTCGGC CGCGTTAAGA
     2101 GCAAGAATGC CCTTAACGCA TCCGCAGATT TATACAACAA TCAGATTGTT GTCGTTAGTC
     2161 AAGAATCTCC CCTTGAAGAA GAACCCAATC TTGAGCATTT GTTCTACTTA GGAACGGTCG
     2221 CGGACCTGTC CGTTAAGAAA GTTTGGAAAG ATGGTACAAT CAGCGTCGAG CTTAATTACA
     2281 ATCAGAAAAT CAAAATCGAC GAGTTCGTGG AGGAGGATAA TATTATCTAT GCTATTGGGT
     2341 CGGTATTTGA GGACAAACTT CCAAAAACCG ACGCCCAAAA GACTAAAATC AAAGAAGCTT
     2401 TAGAAGAGTT GCAGGAAAAA CACAGCTTTA ACACCAGTGA GTTACTGTTA GCGTTTAACG
     2461 AGAATGACTT CAACAAGTTG AATTCACTTA TTTACCAGAT TATTGATAAA ATGCCATTAG
     2521 TTAGCTTGAA CACAAAGCTT TTGTTAATTC AGAGTACGTC AATTTTAGAG AAGTTAGACT
     2581 TGTTGAAGGA ACTGATTATC AACCGCCCTA AGTCTACGGT CAAGTTAAAC AACAATTTAA
     2641 ATAACAACAG CACAGTTGAC AGCGAAATCA ATAAGAAACT GAAGGACAAG ATGGATAAAC
     2701 AGCAAAAAGA GTATTATCTG CGTGAGAAGA TGCGCATTAT CAAGGAGGAA TTGGATGACG
     2761 AGAATTCGGA TGCTTCTCAG TTAGACAAAT ACAAAAAGCG CCTGGAAGAG GAACCATTCC
     2821 CTGAATCAGT GAAGGAGAAA ATTTTATCGT CAATTAAACG TATCGAAACG ATGCAGCCCG
     2881 GCTCCGCTGA GGTAAATGTT GAGCGTAATT ACGTAGACTG GATGATGTCG ATCCCTTGGT
     2941 GGGAGCAGAG TGAGGACATC GATGACTTAA AGTACGCCCA AGAGATTTTA GAGAAACATC
     3001 ACTTTGGTTT GAAGAAAGTG AAGGAACGTA TCATTGAGTA TCTGGCGGTA AAACAGAAAA
     3061 CAAAATCGTT GAAAGGGCCA ATTATTACAT TCGTTGGACC GCCCGGAGTT GGTAAAACCT
     3121 CTTTAGCTCG TTCTATTGCG GAGGCATTAG GTAAAAAGTT TGTGAAGGTA TCCTTGGGAG
     3181 GGGTAAAAGA CGAAAGCGAG ATCCGTGGAC ACCGCAAAAC ATATGTTGGT TCAATGCCAG
     3241 GGCGTATCAT CCAGGCCCTT AAACGTGCGA AGGTCAAGAA CCCTCTTTTC CTGTTGGACG
     3301 AGATTGATAA AATGGCGTCC GACAACCGTG GTGATCCGGC TTCCGCTATG CTTGAAGTAC
     3361 TGGATCCAGA ACAAAATAAA GAGTTCTCGG ATCATTACAT CGAAGAACCT TATGACTTAT
     3421 CAACCGTTAT GTTCATTGCA ACCGCCAACT ACATTGAAAA TATCCCGGAG GCTTTATACG
     3481 ACCGCATGGA GATTATTAAT CTGAGTAGTT ACACAGAAAT CGAAAAGATG CATATCGCCA
     3541 AAGATTATCT GACTAAGAAA ATTTTAGAAG AGGACCAATT GACAGAAGAT GAGCTTCGTT
     3601 TCACGGACGA GGCTTACGAT GAAATTATCA AATATTATAC CCGTGAAGCA GGGGTGCGTC
     3661 AGTTAGAGCG CCACCTGGCG ACGATCGCGC GTAAATTCAT TGTTAAGTTG TTGAACGGCA
     3721 AAATCACCAA TCTTGTTGTT ACACGCGAAG TCGTGGTTCA GTATTTGGGA AAACACATCT
     3781 TCGAGCATAC CTCTAAGGAG GAAGAGAGTC AAGTGGGAGT GGTCACTGGT TTAGCATACA
     3841 CTCAGTTTGG AGGTGACATC TTGCCCATCG AAGTCTCTAC TTATAATGGC AAGGGAAACT
     3901 TGACGCTGAC TGGAAAGTTA GGTGAAGTCA TGAAAGAGAG CGCGACGATT GCTTTGACAT
     3961 ACGTGAAAGC TAACCATGAA AAATTCGGGA TCTCAAAGGA TAAATTTGAT GACATCGACA
     4021 TCCACATTCA TGTCCCGGAG GGAGCCGTGC CTAAAGATGG GCCATCAGCC GGCATCACTT
     4081 TAACCACTGC ACTGATCAGT GCCCTGTCTA AACAACCAGT CTCGAAAGAT TTCGGCATGA
     4141 CCGGAGAGAT CACGTTACGC GGAAATGTCC TGCCGATCGG CGGATTACGT GAGAAATCTA
     4201 TCAGTGCAGC ACGCAGTGGG TTGAAGCACA TTTTGATCCC ATCAAAGAAC GTGAAAGACA
     4261 TCGAGGACGT CCCACAAGAG GTACAGGACG TGTTGAAAAT CACGCCAGTG AGCAAATACG
     4321 AGGATGTGTA TGAAATCATT TTTAAGAATA ACAATCAGCA AACAATTTGA AGAGCTTAGA
     4381 GACC
//