LOCUS Exported 4384 bp DNA circular SYN 24-JUL-2020 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2001) AUTHORS Keoni Gandall TITLE Direct Submission JOURNAL Exported from Poly. FEATURES Location/Qualifiers terminator 1..4384 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" terminator 38..85 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" primer_bind 1933..1949 /label="M13 for" /note="common sequencing primer, one of multiple similar variants" terminator 1950..1981 /label="tonB terminator" /note="bidirectional E. coli tonB-P14 transcription terminator" CDS 2010..4370 /label="MMSYN1_0394" /note="lon: endopeptidase La" /translation="MKTIKLPVVVTRGIFILPSTSKTIEFGRVKSKNALNASADLYNNQIVVVSQESPLEEEPNLEHLFYLGTVADLSVKKVWKDGTISVELNYNQKIKIDEFVEEDNIIYAIGSVFEDKLPKTDAQKTKIKEALEELQEKHSFNTSELLLAFNENDFNKLNSLIYQIIDKMPLVSLNTKLLLIQSTSILEKLDLLKELIINRPKSTVKLNNNLNNNSTVDSEINKKLKDKMDKQQKEYYLREKMRIIKEELDDENSDASQLDKYKKRLEEEPFPESVKEKILSSIKRIETMQPGSAEVNVERNYVDWMMSIPWWEQSEDIDDLKYAQEILEKHHFGLKKVKERIIEYLAVKQKTKSLKGPIITFVGPPGVGKTSLARSIAEALGKKFVKVSLGGVKDESEIRGHRKTYVGSMPGRIIQALKRAKVKNPLFLLDEIDKMASDNRGDPASAMLEVLDPEQNKEFSDHYIEEPYDLSTVMFIATANYIENIPEALYDRMEIINLSSYTEIEKMHIAKDYLTKKILEEDQLTEDELRFTDEAYDEIIKYYTREAGVRQLERHLATIARKFIVKLLNGKITNLVVTREVVVQYLGKHIFEHTSKEEESQVGVVTGLAYTQFGGDILPIEVSTYNGKGNLTLTGKLGEVMKESATIALTYVKANHEKFGISKDKFDDIDIHIHVPEGAVPKDGPSAGITLTTALISALSKQPVSKDFGMTGEITLRGNVLPIGGLREKSISAARSGLKHILIPSKNVKDIEDVPQEVQDVLKITPVSKYEDVYEIIFKNNNQQTI" primer_bind complement(90..106) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" rep_origin complement(173..761) /label="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(952..1812) /label="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*" promoter complement(1813..1917) /label="bla promoter" /note="ampicillin resistance gene promoter" ORIGIN 1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC 61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT 121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA 181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC 241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG 301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC 361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG 421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC 481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA 541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG 601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA 661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG 721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA 781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA 841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA 901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG 961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG 1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC 1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC 1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA 1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC 1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG 1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC 1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT 1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG 1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC 1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG 1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT 1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG 1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG 1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT 1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC 1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC 1981 TTGGTTCAGG TGGAGTGGGA GGGTCTCAAA TGAAAACAAT TAAGCTTCCG GTTGTTGTAA 2041 CTCGCGGCAT TTTCATCCTT CCCTCGACAT CTAAGACAAT CGAATTCGGC CGCGTTAAGA 2101 GCAAGAATGC CCTTAACGCA TCCGCAGATT TATACAACAA TCAGATTGTT GTCGTTAGTC 2161 AAGAATCTCC CCTTGAAGAA GAACCCAATC TTGAGCATTT GTTCTACTTA GGAACGGTCG 2221 CGGACCTGTC CGTTAAGAAA GTTTGGAAAG ATGGTACAAT CAGCGTCGAG CTTAATTACA 2281 ATCAGAAAAT CAAAATCGAC GAGTTCGTGG AGGAGGATAA TATTATCTAT GCTATTGGGT 2341 CGGTATTTGA GGACAAACTT CCAAAAACCG ACGCCCAAAA GACTAAAATC AAAGAAGCTT 2401 TAGAAGAGTT GCAGGAAAAA CACAGCTTTA ACACCAGTGA GTTACTGTTA GCGTTTAACG 2461 AGAATGACTT CAACAAGTTG AATTCACTTA TTTACCAGAT TATTGATAAA ATGCCATTAG 2521 TTAGCTTGAA CACAAAGCTT TTGTTAATTC AGAGTACGTC AATTTTAGAG AAGTTAGACT 2581 TGTTGAAGGA ACTGATTATC AACCGCCCTA AGTCTACGGT CAAGTTAAAC AACAATTTAA 2641 ATAACAACAG CACAGTTGAC AGCGAAATCA ATAAGAAACT GAAGGACAAG ATGGATAAAC 2701 AGCAAAAAGA GTATTATCTG CGTGAGAAGA TGCGCATTAT CAAGGAGGAA TTGGATGACG 2761 AGAATTCGGA TGCTTCTCAG TTAGACAAAT ACAAAAAGCG CCTGGAAGAG GAACCATTCC 2821 CTGAATCAGT GAAGGAGAAA ATTTTATCGT CAATTAAACG TATCGAAACG ATGCAGCCCG 2881 GCTCCGCTGA GGTAAATGTT GAGCGTAATT ACGTAGACTG GATGATGTCG ATCCCTTGGT 2941 GGGAGCAGAG TGAGGACATC GATGACTTAA AGTACGCCCA AGAGATTTTA GAGAAACATC 3001 ACTTTGGTTT GAAGAAAGTG AAGGAACGTA TCATTGAGTA TCTGGCGGTA AAACAGAAAA 3061 CAAAATCGTT GAAAGGGCCA ATTATTACAT TCGTTGGACC GCCCGGAGTT GGTAAAACCT 3121 CTTTAGCTCG TTCTATTGCG GAGGCATTAG GTAAAAAGTT TGTGAAGGTA TCCTTGGGAG 3181 GGGTAAAAGA CGAAAGCGAG ATCCGTGGAC ACCGCAAAAC ATATGTTGGT TCAATGCCAG 3241 GGCGTATCAT CCAGGCCCTT AAACGTGCGA AGGTCAAGAA CCCTCTTTTC CTGTTGGACG 3301 AGATTGATAA AATGGCGTCC GACAACCGTG GTGATCCGGC TTCCGCTATG CTTGAAGTAC 3361 TGGATCCAGA ACAAAATAAA GAGTTCTCGG ATCATTACAT CGAAGAACCT TATGACTTAT 3421 CAACCGTTAT GTTCATTGCA ACCGCCAACT ACATTGAAAA TATCCCGGAG GCTTTATACG 3481 ACCGCATGGA GATTATTAAT CTGAGTAGTT ACACAGAAAT CGAAAAGATG CATATCGCCA 3541 AAGATTATCT GACTAAGAAA ATTTTAGAAG AGGACCAATT GACAGAAGAT GAGCTTCGTT 3601 TCACGGACGA GGCTTACGAT GAAATTATCA AATATTATAC CCGTGAAGCA GGGGTGCGTC 3661 AGTTAGAGCG CCACCTGGCG ACGATCGCGC GTAAATTCAT TGTTAAGTTG TTGAACGGCA 3721 AAATCACCAA TCTTGTTGTT ACACGCGAAG TCGTGGTTCA GTATTTGGGA AAACACATCT 3781 TCGAGCATAC CTCTAAGGAG GAAGAGAGTC AAGTGGGAGT GGTCACTGGT TTAGCATACA 3841 CTCAGTTTGG AGGTGACATC TTGCCCATCG AAGTCTCTAC TTATAATGGC AAGGGAAACT 3901 TGACGCTGAC TGGAAAGTTA GGTGAAGTCA TGAAAGAGAG CGCGACGATT GCTTTGACAT 3961 ACGTGAAAGC TAACCATGAA AAATTCGGGA TCTCAAAGGA TAAATTTGAT GACATCGACA 4021 TCCACATTCA TGTCCCGGAG GGAGCCGTGC CTAAAGATGG GCCATCAGCC GGCATCACTT 4081 TAACCACTGC ACTGATCAGT GCCCTGTCTA AACAACCAGT CTCGAAAGAT TTCGGCATGA 4141 CCGGAGAGAT CACGTTACGC GGAAATGTCC TGCCGATCGG CGGATTACGT GAGAAATCTA 4201 TCAGTGCAGC ACGCAGTGGG TTGAAGCACA TTTTGATCCC ATCAAAGAAC GTGAAAGACA 4261 TCGAGGACGT CCCACAAGAG GTACAGGACG TGTTGAAAAT CACGCCAGTG AGCAAATACG 4321 AGGATGTGTA TGAAATCATT TTTAAGAATA ACAATCAGCA AACAATTTGA AGAGCTTAGA 4381 GACC //