LOCUS       Exported     4486 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..4486
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      38..85
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     1933..1949
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      1950..1981
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             2010..4472
                     /label="MMSYN1_0398"
                     /note=""
                     /translation="MKKILIGLSTFSLLVSSSSIVSCTITYQFKNNYLDQLKMILNTSSIAAQSIILSDKNTTNISTDYSLKTFSQTKINDLYKNEEKKLADKYVIDKKATYEYQFKSMFLSLENQKWTETLKKITTIDKNNQTTNLDLAWNDQNTKTTDNNIFKTLSLASAGFNFLFSGDFTPNQQGDLINNFLSNQFGLLESTVFKDNQFSNLIDQLNNIDNNQFYNLTNSLLTQPEWLNSDKENNLTKKTLKEILESSSKKLWDQILPKDGKQDFKIDWSKVFKPLIDLLKAFSIYYEQVEQRSDKNLTYQTIDPLHLFIKEKTNSEFLYEVLNTDLQTIYKNKSEDQIKQEINSINLKKIISFLKNTLVFDKEDKHGYKFQKFVVILLGSASQKESQNDITNNFLLKPFYTWYEKNEELVKKIITSKLEKIESIKPYASFVSNITPILFKVIKAFHQDLTEQGLNKKLSSELSSYLSLAKTLLPTLSVDKKVIDFLDSKSLKDFLNNPFLALYKQNFLKEVFQLINQLSNKEVINNQIIDNVSNVYNLTTLKLDKLLNYLLELIKKPSPSKTSLDEFQFLYGLKDLSISQIINNLSTFYNKENLDYIFNLSNFKNLLEAIFNKNITMSFKYKNQEKELKTQNNLSTILAILGLNSNYTKDLKIEIKDDKNNISQKIKQLIEQKQYGLISVILLGFDADKKQFYKDSILDNIANLFGHNDKDINKEASKNAINILIKSYLELINWFQNVSLKKYAKDNFSTYLDQNNWSTELIDKKGNIENLSKPLIIDYMLKYKNPKDDNQNWKFKVSITRTSDFEQPWKISEITKLTNN"
     primer_bind     complement(90..106)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(173..761)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(952..1812)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(1813..1917)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC
       61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT
      121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA
      181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC
      241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG
      301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC
      361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG
      421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC
      481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA
      541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG
      601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA
      661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG
      721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA
      781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA
      841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA
      901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG
      961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG
     1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC
     1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC
     1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA
     1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC
     1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG
     1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC
     1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT
     1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG
     1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC
     1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG
     1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT
     1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG
     1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG
     1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT
     1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC
     1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC
     1981 TTGGTTCAGG TGGAGTGGGA GGGTCTCAAA TGAAAAAGAT CCTTATCGGA CTTTCTACAT
     2041 TCTCCTTATT AGTATCTTCT TCGAGCATTG TATCGTGCAC AATCACGTAC CAGTTTAAAA
     2101 ACAATTACTT GGATCAGTTG AAGATGATCC TTAATACTAG TTCTATTGCT GCGCAATCAA
     2161 TCATCTTATC AGATAAGAAC ACCACCAATA TTTCAACGGA TTACAGCCTC AAGACATTCT
     2221 CACAGACTAA AATCAACGAC TTATATAAAA ACGAGGAGAA GAAGTTAGCG GATAAATATG
     2281 TGATCGACAA GAAAGCAACG TATGAGTACC AGTTTAAATC CATGTTCCTG TCCCTTGAAA
     2341 ACCAAAAATG GACGGAAACA TTGAAGAAAA TCACAACTAT CGACAAGAAC AATCAGACAA
     2401 CAAACTTGGA TCTTGCCTGG AATGACCAGA ACACGAAAAC CACCGATAAT AATATCTTTA
     2461 AGACTCTGTC ATTGGCCAGT GCCGGCTTTA ACTTTCTTTT TTCAGGGGAT TTCACCCCCA
     2521 ATCAGCAAGG GGATTTGATT AACAACTTTT TATCCAACCA ATTTGGCTTA CTGGAGTCAA
     2581 CAGTGTTCAA GGATAATCAA TTTTCTAACT TAATCGACCA ATTAAATAAC ATTGATAACA
     2641 ACCAGTTCTA CAATCTGACA AATTCCTTAT TGACACAACC AGAATGGTTG AATAGTGATA
     2701 AAGAAAATAA CCTGACGAAA AAGACTTTGA AGGAAATCTT GGAAAGTTCG TCCAAGAAAC
     2761 TGTGGGATCA GATCTTGCCC AAGGATGGAA AACAGGATTT TAAAATCGAC TGGAGTAAAG
     2821 TATTTAAACC TTTAATTGAC TTACTGAAGG CCTTCAGCAT TTATTATGAA CAGGTTGAGC
     2881 AGCGCTCGGA CAAAAATCTT ACATACCAAA CAATTGATCC TTTACACTTA TTTATTAAGG
     2941 AAAAGACAAA TTCAGAGTTC CTTTACGAGG TGTTGAACAC AGACTTACAA ACGATCTACA
     3001 AAAATAAGAG TGAGGATCAG ATCAAACAGG AGATCAACTC CATCAATTTG AAGAAGATCA
     3061 TCTCCTTTCT TAAGAACACC TTAGTTTTCG ACAAAGAGGA CAAACACGGG TATAAGTTTC
     3121 AGAAATTCGT CGTCATTTTG TTAGGCTCGG CTTCACAAAA GGAGTCACAA AATGATATCA
     3181 CAAATAACTT TTTACTTAAA CCTTTCTATA CATGGTACGA AAAGAATGAA GAATTAGTGA
     3241 AGAAGATTAT TACAAGTAAA TTAGAGAAGA TCGAATCGAT TAAACCCTAC GCCTCGTTCG
     3301 TGAGCAATAT CACGCCAATC TTGTTTAAAG TTATTAAGGC TTTTCATCAG GATTTAACAG
     3361 AACAAGGACT GAACAAGAAG TTATCCTCTG AGTTGTCGTC ATACTTATCT TTGGCTAAAA
     3421 CACTTCTGCC AACGTTAAGT GTTGATAAGA AGGTAATTGA TTTTCTGGAT AGTAAATCTT
     3481 TGAAAGACTT CTTGAATAAC CCGTTCCTTG CATTGTATAA ACAGAATTTC CTGAAGGAGG
     3541 TGTTCCAGCT TATTAATCAG CTTTCGAATA AGGAAGTGAT TAATAATCAG ATCATTGACA
     3601 ATGTCTCGAA CGTTTACAAT CTTACGACTT TAAAACTTGA CAAGCTGCTT AATTATCTTT
     3661 TAGAACTTAT TAAGAAACCT TCCCCAAGTA AAACCTCCTT AGACGAATTT CAGTTCTTAT
     3721 ATGGATTAAA GGACTTATCG ATCAGTCAGA TCATTAACAA CCTGTCCACA TTTTATAATA
     3781 AGGAGAACCT TGATTATATC TTCAACTTAT CTAATTTTAA GAATCTTCTG GAAGCAATTT
     3841 TTAATAAAAA CATCACCATG AGTTTTAAGT ACAAAAATCA AGAGAAAGAA TTGAAAACGC
     3901 AAAATAATCT GTCTACCATT TTAGCAATTC TTGGTTTGAA TTCTAATTAT ACCAAGGATT
     3961 TAAAAATCGA AATCAAAGAC GATAAAAACA ATATCTCCCA GAAGATTAAA CAGTTAATCG
     4021 AGCAGAAGCA ATACGGCTTG ATTAGCGTGA TTCTGTTGGG TTTTGACGCG GATAAAAAAC
     4081 AGTTTTATAA AGACTCAATC TTAGATAACA TTGCTAATCT GTTTGGGCAT AATGACAAGG
     4141 ACATCAACAA AGAAGCATCC AAGAACGCAA TTAACATCCT GATTAAGAGC TACCTTGAAT
     4201 TGATTAATTG GTTCCAGAAC GTTTCCCTGA AGAAATACGC TAAAGACAAT TTTTCTACCT
     4261 ATCTTGATCA AAATAACTGG AGTACAGAGC TTATTGACAA AAAAGGTAAT ATTGAAAACT
     4321 TATCTAAGCC CCTTATTATC GATTACATGC TTAAGTATAA GAACCCAAAA GATGACAACC
     4381 AGAATTGGAA ATTTAAGGTT TCAATCACCC GCACGTCTGA CTTCGAGCAG CCTTGGAAGA
     4441 TCTCCGAGAT TACTAAGCTG ACCAACAACT GAAGAGCTTA GAGACC
//