LOCUS       Exported     3808 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3808
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      38..85
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     1933..1949
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      1950..1981
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             2010..3794
                     /label="MMSYN1_0401"
                     /note="peptidase, C39 family"
                     /translation="MIINYYYNQNYDLDRLKLEINYVEEMLSFYDISNICSKYFLTCKALQIENDLEQINKKVYLAQVVNQTGLLHFVVVEKQNNHLIIYDPLKTKKQKFTYKDFYQIFTGYILIFNSNYKKFKANYNNLFTLFDSFYLAYLFYIILNIFSILLTILEMRFLYVYSLSITNLNNSYFLYLYFLAIFIINIFLNEISKFLLNKYYQKNKSKKLETFYYYLVEKNIKLDIINTYSEIEFISSYQTYVLLNTISAVINSLVILFVIFYINKTIFLVLFVFDLFWLVISFIYNFFTNQNKTNNQNLNLITHLLNKTKLIDKKTSLELIKKDLNKTQTDYLHILFNFFEKISLLVIYYISWDLLKFNYIEFSILLIIVLFKAIHTNDLKKLVYFLQNFNKYKQLLIKFNNFKLANNYIELEQINNIQIRNLLTNLDINLDQKINYLSNEYDLKTFIKTKNSNDHILILINKINLKDISTFSLNKHFIHLDNLEIKYSTILQNIIINQSDLNIFTHKIIKDLINKYQINLTKIINLETITKLETEFIKLLRIFYLDHHYLLFNDNFEIINKTDISLVLKLFTSYSNSSLIITSNDIKYNLISKD"
     primer_bind     complement(90..106)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(173..761)
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
                     /label="ori"
     CDS             complement(952..1812)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(1813..1917)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC
       61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT
      121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA
      181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC
      241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG
      301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC
      361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG
      421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC
      481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA
      541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG
      601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA
      661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG
      721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA
      781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA
      841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA
      901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG
      961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG
     1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC
     1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC
     1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA
     1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC
     1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG
     1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC
     1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT
     1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG
     1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC
     1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG
     1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT
     1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG
     1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG
     1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT
     1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC
     1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC
     1981 TTGGTTCAGG TGGAGTGGGA GGGTCTCAAA TGATTATTAA TTACTACTAT AATCAAAACT
     2041 ACGACTTAGA CCGCCTGAAG CTGGAAATTA ACTATGTCGA GGAAATGTTG TCATTTTATG
     2101 ATATCAGCAA CATCTGTTCG AAATATTTCT TAACTTGTAA AGCCCTTCAG ATCGAGAATG
     2161 ATCTTGAGCA AATCAATAAG AAAGTATACC TGGCTCAGGT CGTGAACCAA ACCGGGTTAC
     2221 TGCATTTCGT CGTAGTAGAG AAGCAGAACA ACCATTTGAT CATTTATGAC CCGCTTAAAA
     2281 CCAAGAAGCA AAAATTCACC TACAAGGATT TCTATCAAAT CTTCACAGGC TATATTTTGA
     2341 TCTTTAACTC AAACTACAAA AAGTTCAAGG CAAATTATAA TAACTTATTC ACACTGTTTG
     2401 ACTCCTTCTA TTTAGCTTAT CTGTTCTATA TTATCCTGAA CATTTTTAGT ATTTTATTGA
     2461 CGATCTTAGA AATGCGCTTC CTTTACGTTT ATAGCCTGTC CATTACCAAC CTGAACAACA
     2521 GTTATTTCTT ATACCTTTAC TTTCTTGCTA TCTTCATCAT CAATATTTTC TTAAACGAGA
     2581 TTAGTAAATT CCTGCTGAAT AAATATTATC AGAAAAATAA GTCCAAAAAG CTTGAGACAT
     2641 TCTACTACTA CTTGGTTGAG AAAAACATCA AACTGGACAT TATTAATACT TATAGCGAAA
     2701 TTGAATTTAT CAGCTCGTAC CAAACTTACG TTTTGCTTAA TACCATCAGT GCAGTAATCA
     2761 ATTCGCTGGT GATCTTGTTC GTCATCTTCT ACATCAATAA AACTATTTTC TTAGTCTTAT
     2821 TTGTATTCGA CTTGTTCTGG CTGGTGATCT CATTCATTTA TAACTTTTTT ACAAATCAGA
     2881 ACAAAACGAA TAACCAAAAT TTGAACTTGA TCACGCATTT ATTAAACAAA ACGAAGCTGA
     2941 TTGACAAAAA GACTAGTCTG GAGTTAATTA AAAAGGATTT AAACAAAACG CAGACGGACT
     3001 ACTTGCACAT TCTTTTCAAT TTTTTCGAGA AGATCTCGCT TCTTGTCATC TACTATATTA
     3061 GTTGGGATTT GTTGAAATTT AATTATATTG AGTTTTCCAT CTTACTTATC ATTGTATTGT
     3121 TCAAAGCTAT CCACACCAAT GACCTTAAAA AATTGGTGTA TTTTTTACAG AACTTCAATA
     3181 AGTATAAGCA GCTTTTAATC AAATTTAACA ATTTCAAGTT AGCAAATAAC TATATCGAAT
     3241 TGGAACAGAT CAATAACATT CAAATCCGCA ACTTGCTGAC CAATTTGGAT ATTAATTTGG
     3301 ATCAGAAAAT TAATTACCTT AGTAACGAGT ACGACTTAAA AACCTTCATC AAGACTAAGA
     3361 ACTCCAATGA TCACATTTTG ATCTTGATTA ACAAAATCAA CTTAAAAGAT ATTTCGACCT
     3421 TTAGTTTGAA CAAACATTTT ATCCACCTGG ACAACTTGGA AATCAAATAC TCCACGATCC
     3481 TGCAGAATAT TATTATCAAC CAAAGCGACT TAAATATCTT TACTCATAAA ATCATTAAAG
     3541 ACCTTATCAA TAAGTATCAG ATTAATCTGA CAAAAATCAT CAACCTTGAA ACGATCACGA
     3601 AATTAGAAAC CGAGTTCATT AAACTTCTGC GTATTTTCTA TCTGGATCAT CATTATCTTT
     3661 TATTTAACGA TAATTTCGAA ATTATTAATA AGACAGACAT CTCGCTGGTG CTTAAGCTGT
     3721 TCACCAGTTA CTCGAATTCT TCATTAATCA TTACGTCCAA CGACATCAAA TACAACCTGA
     3781 TTAGCAAGGA TTGAAGAGCT TAGAGACC
//