LOCUS Exported 3808 bp DNA circular SYN 24-JUL-2020 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2001) AUTHORS Keoni Gandall TITLE Direct Submission JOURNAL Exported from Poly. FEATURES Location/Qualifiers terminator 1..3808 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" terminator 38..85 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" primer_bind 1933..1949 /label="M13 for" /note="common sequencing primer, one of multiple similar variants" terminator 1950..1981 /label="tonB terminator" /note="bidirectional E. coli tonB-P14 transcription terminator" CDS 2010..3794 /label="MMSYN1_0401" /note="peptidase, C39 family" /translation="MIINYYYNQNYDLDRLKLEINYVEEMLSFYDISNICSKYFLTCKALQIENDLEQINKKVYLAQVVNQTGLLHFVVVEKQNNHLIIYDPLKTKKQKFTYKDFYQIFTGYILIFNSNYKKFKANYNNLFTLFDSFYLAYLFYIILNIFSILLTILEMRFLYVYSLSITNLNNSYFLYLYFLAIFIINIFLNEISKFLLNKYYQKNKSKKLETFYYYLVEKNIKLDIINTYSEIEFISSYQTYVLLNTISAVINSLVILFVIFYINKTIFLVLFVFDLFWLVISFIYNFFTNQNKTNNQNLNLITHLLNKTKLIDKKTSLELIKKDLNKTQTDYLHILFNFFEKISLLVIYYISWDLLKFNYIEFSILLIIVLFKAIHTNDLKKLVYFLQNFNKYKQLLIKFNNFKLANNYIELEQINNIQIRNLLTNLDINLDQKINYLSNEYDLKTFIKTKNSNDHILILINKINLKDISTFSLNKHFIHLDNLEIKYSTILQNIIINQSDLNIFTHKIIKDLINKYQINLTKIINLETITKLETEFIKLLRIFYLDHHYLLFNDNFEIINKTDISLVLKLFTSYSNSSLIITSNDIKYNLISKD" primer_bind complement(90..106) /label="M13 rev" /note="common sequencing primer, one of multiple similar variants" rep_origin complement(173..761) /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(952..1812) /label="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*" promoter complement(1813..1917) /label="bla promoter" /note="ampicillin resistance gene promoter" ORIGIN 1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC 61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT 121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA 181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC 241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG 301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC 361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG 421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC 481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA 541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG 601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA 661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG 721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA 781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA 841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA 901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG 961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG 1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC 1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC 1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA 1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC 1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG 1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC 1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT 1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG 1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC 1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG 1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT 1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG 1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG 1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT 1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC 1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC 1981 TTGGTTCAGG TGGAGTGGGA GGGTCTCAAA TGATTATTAA TTACTACTAT AATCAAAACT 2041 ACGACTTAGA CCGCCTGAAG CTGGAAATTA ACTATGTCGA GGAAATGTTG TCATTTTATG 2101 ATATCAGCAA CATCTGTTCG AAATATTTCT TAACTTGTAA AGCCCTTCAG ATCGAGAATG 2161 ATCTTGAGCA AATCAATAAG AAAGTATACC TGGCTCAGGT CGTGAACCAA ACCGGGTTAC 2221 TGCATTTCGT CGTAGTAGAG AAGCAGAACA ACCATTTGAT CATTTATGAC CCGCTTAAAA 2281 CCAAGAAGCA AAAATTCACC TACAAGGATT TCTATCAAAT CTTCACAGGC TATATTTTGA 2341 TCTTTAACTC AAACTACAAA AAGTTCAAGG CAAATTATAA TAACTTATTC ACACTGTTTG 2401 ACTCCTTCTA TTTAGCTTAT CTGTTCTATA TTATCCTGAA CATTTTTAGT ATTTTATTGA 2461 CGATCTTAGA AATGCGCTTC CTTTACGTTT ATAGCCTGTC CATTACCAAC CTGAACAACA 2521 GTTATTTCTT ATACCTTTAC TTTCTTGCTA TCTTCATCAT CAATATTTTC TTAAACGAGA 2581 TTAGTAAATT CCTGCTGAAT AAATATTATC AGAAAAATAA GTCCAAAAAG CTTGAGACAT 2641 TCTACTACTA CTTGGTTGAG AAAAACATCA AACTGGACAT TATTAATACT TATAGCGAAA 2701 TTGAATTTAT CAGCTCGTAC CAAACTTACG TTTTGCTTAA TACCATCAGT GCAGTAATCA 2761 ATTCGCTGGT GATCTTGTTC GTCATCTTCT ACATCAATAA AACTATTTTC TTAGTCTTAT 2821 TTGTATTCGA CTTGTTCTGG CTGGTGATCT CATTCATTTA TAACTTTTTT ACAAATCAGA 2881 ACAAAACGAA TAACCAAAAT TTGAACTTGA TCACGCATTT ATTAAACAAA ACGAAGCTGA 2941 TTGACAAAAA GACTAGTCTG GAGTTAATTA AAAAGGATTT AAACAAAACG CAGACGGACT 3001 ACTTGCACAT TCTTTTCAAT TTTTTCGAGA AGATCTCGCT TCTTGTCATC TACTATATTA 3061 GTTGGGATTT GTTGAAATTT AATTATATTG AGTTTTCCAT CTTACTTATC ATTGTATTGT 3121 TCAAAGCTAT CCACACCAAT GACCTTAAAA AATTGGTGTA TTTTTTACAG AACTTCAATA 3181 AGTATAAGCA GCTTTTAATC AAATTTAACA ATTTCAAGTT AGCAAATAAC TATATCGAAT 3241 TGGAACAGAT CAATAACATT CAAATCCGCA ACTTGCTGAC CAATTTGGAT ATTAATTTGG 3301 ATCAGAAAAT TAATTACCTT AGTAACGAGT ACGACTTAAA AACCTTCATC AAGACTAAGA 3361 ACTCCAATGA TCACATTTTG ATCTTGATTA ACAAAATCAA CTTAAAAGAT ATTTCGACCT 3421 TTAGTTTGAA CAAACATTTT ATCCACCTGG ACAACTTGGA AATCAAATAC TCCACGATCC 3481 TGCAGAATAT TATTATCAAC CAAAGCGACT TAAATATCTT TACTCATAAA ATCATTAAAG 3541 ACCTTATCAA TAAGTATCAG ATTAATCTGA CAAAAATCAT CAACCTTGAA ACGATCACGA 3601 AATTAGAAAC CGAGTTCATT AAACTTCTGC GTATTTTCTA TCTGGATCAT CATTATCTTT 3661 TATTTAACGA TAATTTCGAA ATTATTAATA AGACAGACAT CTCGCTGGTG CTTAAGCTGT 3721 TCACCAGTTA CTCGAATTCT TCATTAATCA TTACGTCCAA CGACATCAAA TACAACCTGA 3781 TTAGCAAGGA TTGAAGAGCT TAGAGACC //