LOCUS       Exported     2963 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..2963
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      976..1023
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     2851..2867
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      2868..2899
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
                     /label="tonB terminator"
     CDS             1..0
                     /note="era: GTP-binding protein Era"
                     /translation="MSNFKSGFVSIIGRPNVGKSTLLNKLIGEKISIVTNKPQTTRNNIRGILTKKDQYQIVFIDTPGVHTSKKQLDKFLNTSALKSTKDVDVILFLAPSDEVIGKNDLFLLKQIENLDVFKILVITKADSVTKEQLILKANEWSSYQDQFDEIIITSSITNLNIEKLLELIVNNLSDNDYQFYDDDILTDQSDRFMIKEIIRENILLKTGQEVPHSVAVLVEHLEQNENEINISCAIIVERQSQKSIIIGKNGVKISDIRYKSKKQIQALFKKRINLELFVKVQENWRNSPSLIKKMGYNKDQY"
                     /label="MMSYN1_0403"
     primer_bind     complement(1028..1044)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1111..1699)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(1870..2730)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(2731..2835)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
     CDS             complement(1..0)
                     /label="MMSYN1_0403"
                     /note="era: GTP-binding protein Era"
                     /translation="MSNFKSGFVSIIGRPNVGKSTLLNKLIGEKISIVTNKPQTTRNNIRGILTKKDQYQIVFIDTPGVHTSKKQLDKFLNTSALKSTKDVDVILFLAPSDEVIGKNDLFLLKQIENLDVFKILVITKADSVTKEQLILKANEWSSYQDQFDEIIITSSITNLNIEKLLELIVNNLSDNDYQFYDDDILTDQSDRFMIKEIIRENILLKTGQEVPHSVAVLVEHLEQNENEINISCAIIVERQSQKSIIIGKNGVKISDIRYKSKKQIQALFKKRINLELFVKVQENWRNSPSLIKKMGYNKDQY"
ORIGIN
        1 ATGAGCAATT TCAAATCGGG CTTTGTTAGC ATCATTGGCC GCCCGAATGT GGGGAAAAGC
       61 ACCTTATTAA ACAAGTTAAT TGGCGAGAAG ATTTCGATTG TCACCAACAA GCCACAGACC
      121 ACACGCAATA ATATTCGCGG CATCTTGACG AAGAAGGATC AATACCAGAT CGTGTTCATT
      181 GATACACCAG GAGTCCACAC AAGTAAAAAG CAATTGGACA AATTTCTGAA TACGTCGGCA
      241 CTGAAATCAA CGAAGGACGT AGACGTAATC TTGTTCTTGG CGCCCTCTGA CGAAGTTATC
      301 GGCAAGAATG ACTTGTTTCT GTTAAAGCAA ATCGAAAATC TGGACGTGTT CAAAATCCTT
      361 GTCATTACAA AAGCGGATAG CGTCACCAAA GAACAGCTGA TCTTAAAGGC GAACGAGTGG
      421 TCATCATACC AGGACCAGTT CGATGAAATC ATTATTACTT CATCCATCAC TAACCTTAAC
      481 ATTGAGAAAC TGTTAGAGCT GATTGTAAAC AATCTGTCAG ATAATGATTA TCAATTTTAC
      541 GACGACGATA TCCTGACCGA CCAATCTGAC CGTTTCATGA TCAAAGAGAT TATCCGTGAA
      601 AACATTTTGT TAAAGACCGG GCAGGAGGTC CCTCACTCCG TTGCAGTTTT GGTCGAGCAT
      661 CTTGAACAGA ACGAAAACGA GATCAATATC TCGTGTGCTA TTATTGTGGA ACGTCAAAGC
      721 CAGAAAAGCA TTATTATCGG AAAAAATGGC GTTAAAATCT CTGACATTCG CTATAAAAGT
      781 AAAAAGCAAA TTCAGGCACT GTTCAAAAAG CGCATCAATC TTGAGTTGTT TGTTAAAGTG
      841 CAGGAAAATT GGCGCAATTC GCCGAGTCTG ATTAAGAAGA TGGGATATAA TAAGGACCAA
      901 TACTGAAGAG CTTAGAGACC GGTACTAGTT CCTGCAGGGC TCGAGGTGTC AATCGTCGGA
      961 GCCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT
     1021 TTGCATGGTC ATAGCTGTTT CCTGAGAGCT TGGCAGGTGA TGACACACAT TAACAAATTT
     1081 CGTGAGGAGT CTCCAGAAGA ATGCCATTAA TTTCCATAGG CTCCGCCCCC CTGACGAGCA
     1141 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA
     1201 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG
     1261 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG
     1321 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT
     1381 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA
     1441 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG
     1501 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT
     1561 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC
     1621 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG
     1681 CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG
     1741 GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA
     1801 GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAAACTTG
     1861 GTCTGACAGT TACCAATGCT TAATCAGTGA GGCACCTATC TCAGCGATCT GTCTATTTCG
     1921 TTCATCCATA GTTGCCTGAC TCCCCGTCGT GTAGATAACT ACGATACGGG AGGGCTTACC
     1981 ATCTGGCCCC AGTGCTGCAA TGATACCGCG AGAACCACGC TCACCGGCTC CAGATTTATC
     2041 AGCAATAAAC CAGCCAGCCG GAAGGGCCGA GCGCAGAAGT GGTCCTGCAA CTTTATCCGC
     2101 CTCCATCCAG TCTATTAATT GTTGCCGGGA AGCTAGAGTA AGTAGTTCGC CAGTTAATAG
     2161 TTTGCGCAAC GTTGTTGCCA TTGCTACAGG CATCGTGGTG TCACGCTCGT CGTTTGGTAT
     2221 GGCTTCATTC AGCTCCGGTT CCCAACGATC AAGGCGAGTT ACATGATCCC CCATGTTGTG
     2281 CAAAAAAGCG GTTAGCTCCT TCGGTCCTCC GATCGTTGTC AGAAGTAAGT TGGCCGCAGT
     2341 GTTATCACTC ATGGTTATGG CAGCACTGCA TAATTCTCTT ACTGTCATGC CATCCGTAAG
     2401 ATGCTTTTCT GTGACTGGTG AGTACTCAAC CAAGTCATTC TGAGAATAGT GTATGCGGCG
     2461 ACCGAGTTGC TCTTGCCCGG CGTCAATACG GGATAATACC GCGCCACATA GCAGAACTTT
     2521 AAAAGTGCTC ATCATTGGAA AACGTTCTTC GGGGCGAAAA CTCTCAAGGA TCTTACCGCT
     2581 GTTGAGATCC AGTTCGATGT AACCCACTCG TGCACCCAAC TGATCTTCAG CATCTTTTAC
     2641 TTTCACCAGC GTTTCTGGGT GAGCAAAAAC AGGAAGGCAA AATGCCGCAA AAAAGGGAAT
     2701 AAGGGCGACA CGGAAATGTT GAATACTCAT ACTCTTCCTT TTTCAATATT ATTGAAGCAT
     2761 TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA
     2821 AATAGGGGTT CCGCGCACCT GCACCAGTCA GTAAAACGAC GGCCAGTAGT CAAAAGCCTC
     2881 CGACCGGAGG CTTTTGACTT GGTCAGGTGG AGTGAATTCG CGGCCGCTTC TAGAGGCTCT
     2941 TCGTAATTAC GAAGAGGTCT CTA
//