LOCUS       Exported     2807 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..2807
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
                     /label="T7 terminator"
     terminator      820..867
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     2695..2711
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      2712..2743
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             1..0
                     /note="Involved in DNA repair and RecF pathway recombination."
                     /translation="MEKTLKGIVLNSFDFQDYDKIITIYSNLYGKISLVCLGVNKIKSKNKYGINYLSYSNFEIFKSKNKFNLSKLKRSELINSFNHISTDFNLYLYANIITSLVLSLDEQIKNYNLYKTLKLSISIINNKPDLGLKVCVLFMFYFLKIIGNQIDLSKCGFCNSKINPIIAISFTNYCSSCKFCYFDDCILIDNQLSNFINSIFKNDFITNLSQEISTNNLNILTRFILNYYQDIVGTYTTSMYLLSTLIRFN"
                     /label="MMSYN1_0404"
     primer_bind     complement(872..888)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(955..1543)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(1714..2574)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(2575..2679)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
     CDS             complement(1..0)
                     /label="MMSYN1_0404"
                     /note="Involved in DNA repair and RecF pathway recombination."
                     /translation="MEKTLKGIVLNSFDFQDYDKIITIYSNLYGKISLVCLGVNKIKSKNKYGINYLSYSNFEIFKSKNKFNLSKLKRSELINSFNHISTDFNLYLYANIITSLVLSLDEQIKNYNLYKTLKLSISIINNKPDLGLKVCVLFMFYFLKIIGNQIDLSKCGFCNSKINPIIAISFTNYCSSCKFCYFDDCILIDNQLSNFINSIFKNDFITNLSQEISTNNLNILTRFILNYYQDIVGTYTTSMYLLSTLIRFN"
ORIGIN
        1 ATGGAAAAAA CCCTGAAGGG CATTGTGTTA AATAGTTTTG ACTTTCAAGA TTACGACAAA
       61 ATTATCACTA TTTATTCGAA CCTTTACGGG AAAATCTCCC TGGTTTGTTT GGGTGTTAAT
      121 AAGATTAAGT CCAAAAATAA GTATGGTATC AACTACTTGT CATATTCTAA TTTCGAAATC
      181 TTCAAATCAA AAAACAAGTT CAACTTATCT AAGTTGAAAC GCAGTGAGCT TATCAACTCA
      241 TTCAATCACA TTAGTACTGA TTTCAACTTG TACCTTTATG CAAACATTAT CACCTCTCTG
      301 GTATTGAGCT TGGACGAACA GATTAAGAAC TACAATCTTT ACAAAACATT AAAATTGTCT
      361 ATCTCGATTA TCAATAATAA ACCAGATCTG GGCTTGAAGG TATGTGTATT GTTTATGTTT
      421 TATTTCCTTA AGATCATCGG CAATCAGATT GACTTGAGTA AATGTGGATT TTGCAATTCC
      481 AAAATCAACC CAATTATTGC GATCTCCTTT ACGAACTACT GCTCATCCTG CAAATTCTGT
      541 TACTTCGATG ATTGCATTCT GATCGATAAT CAGCTGTCAA ACTTTATCAA TTCGATCTTC
      601 AAGAACGACT TTATCACTAA CCTGAGTCAA GAAATTAGTA CAAACAACTT AAATATTCTT
      661 ACACGCTTCA TCCTTAACTA CTATCAGGAC ATCGTGGGTA CCTACACTAC ATCGATGTAC
      721 TTACTGTCGA CTCTTATTCG TTTCAACTGA AGAGCTTAGA GACCGGTACT AGTTCCTGCA
      781 GGGCTCGAGG TGTCAATCGT CGGAGCCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC
      841 TCTAAACGGG TCTTGAGGGG TTTTTTGCAT GGTCATAGCT GTTTCCTGAG AGCTTGGCAG
      901 GTGATGACAC ACATTAACAA ATTTCGTGAG GAGTCTCCAG AAGAATGCCA TTAATTTCCA
      961 TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA
     1021 CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC
     1081 TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC
     1141 GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT
     1201 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG
     1261 TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG
     1321 GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA
     1381 CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG
     1441 AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT
     1501 TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT
     1561 TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG
     1621 ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT
     1681 CTAAAGTATA TATGAGTAAA CTTGGTCTGA CAGTTACCAA TGCTTAATCA GTGAGGCACC
     1741 TATCTCAGCG ATCTGTCTAT TTCGTTCATC CATAGTTGCC TGACTCCCCG TCGTGTAGAT
     1801 AACTACGATA CGGGAGGGCT TACCATCTGG CCCCAGTGCT GCAATGATAC CGCGAGAACC
     1861 ACGCTCACCG GCTCCAGATT TATCAGCAAT AAACCAGCCA GCCGGAAGGG CCGAGCGCAG
     1921 AAGTGGTCCT GCAACTTTAT CCGCCTCCAT CCAGTCTATT AATTGTTGCC GGGAAGCTAG
     1981 AGTAAGTAGT TCGCCAGTTA ATAGTTTGCG CAACGTTGTT GCCATTGCTA CAGGCATCGT
     2041 GGTGTCACGC TCGTCGTTTG GTATGGCTTC ATTCAGCTCC GGTTCCCAAC GATCAAGGCG
     2101 AGTTACATGA TCCCCCATGT TGTGCAAAAA AGCGGTTAGC TCCTTCGGTC CTCCGATCGT
     2161 TGTCAGAAGT AAGTTGGCCG CAGTGTTATC ACTCATGGTT ATGGCAGCAC TGCATAATTC
     2221 TCTTACTGTC ATGCCATCCG TAAGATGCTT TTCTGTGACT GGTGAGTACT CAACCAAGTC
     2281 ATTCTGAGAA TAGTGTATGC GGCGACCGAG TTGCTCTTGC CCGGCGTCAA TACGGGATAA
     2341 TACCGCGCCA CATAGCAGAA CTTTAAAAGT GCTCATCATT GGAAAACGTT CTTCGGGGCG
     2401 AAAACTCTCA AGGATCTTAC CGCTGTTGAG ATCCAGTTCG ATGTAACCCA CTCGTGCACC
     2461 CAACTGATCT TCAGCATCTT TTACTTTCAC CAGCGTTTCT GGGTGAGCAA AAACAGGAAG
     2521 GCAAAATGCC GCAAAAAAGG GAATAAGGGC GACACGGAAA TGTTGAATAC TCATACTCTT
     2581 CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT
     2641 TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC ACCTGCACCA GTCAGTAAAA
     2701 CGACGGCCAG TAGTCAAAAG CCTCCGACCG GAGGCTTTTG ACTTGGTCAG GTGGAGTGAA
     2761 TTCGCGGCCG CTTCTAGAGG CTCTTCGTAA TTACGAAGAG GTCTCTA
//