LOCUS       Exported     3394 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3394
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      38..85
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
                     /label="T7 terminator"
     primer_bind     1933..1949
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      1950..1981
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             2010..3380
                     /label="MMSYN1_0405"
                     /note="GlyRS: Glycyl-tRNA synthetase"
                     /translation="MSKSIEKMISHLKNQGFVFQGSEIYGGLANSWDYGPLGCEVKNKLKKVWWDFFVKKNPLNVGLDSSIILNSKVWKASGHIDGFNDPLIDCKNCKSRWRADKLIEEFDSSINTGIMTSAQLEDYIKQNNILCKKCQKKDFTQIRKFALMFKTNQGVLEDDSSIVYLRPETAQGIFINFKNVQRSMRKKLPFGIGQIGKSFRNEITPGNFIFRTREFEQMELEFFFDPNDQRDWFKYWLDQIELFLTKKICLDKSNYKIRENLKDELAHYALKTSDIEFNFPFGWGELWGISHRSDFDLKVHQNLSKEDLTVLKEETNQKVLANVIEPSVGVERLMLAIFWQAYTEEQLEENNSRTVLKLPYNLAPYQVAITPLSKQLNQNANQLFLDLLNDFDAVYDETGNIGKRYRRQDAIGTPFVITYDFQTLEDQKVTIRYRDTMKQERILISQLKDFLNSQFN"
     primer_bind     complement(90..106)
                     /note="common sequencing primer, one of multiple similar variants"
                     /label="M13 rev"
     rep_origin      complement(173..761)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(952..1812)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(1813..1917)
                     /note="ampicillin resistance gene promoter"
                     /label="bla promoter"
ORIGIN
        1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC
       61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT
      121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA
      181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC
      241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG
      301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC
      361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG
      421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC
      481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA
      541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG
      601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA
      661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG
      721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA
      781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA
      841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA
      901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG
      961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG
     1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC
     1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC
     1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA
     1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC
     1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG
     1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC
     1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT
     1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG
     1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC
     1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG
     1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT
     1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG
     1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG
     1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT
     1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC
     1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC
     1981 TTGGTTCAGG TGGAGTGGGA GGGTCTCAAA TGTCAAAAAG TATCGAGAAA ATGATCTCGC
     2041 ACTTAAAGAA CCAGGGGTTC GTCTTTCAGG GATCCGAAAT CTATGGGGGC TTAGCCAACT
     2101 CATGGGACTA CGGCCCCCTT GGATGTGAGG TCAAAAACAA GCTGAAGAAA GTCTGGTGGG
     2161 ATTTTTTCGT CAAGAAAAAC CCCTTAAATG TAGGGTTAGA TTCCAGCATC ATCCTGAACT
     2221 CCAAAGTGTG GAAGGCCTCT GGACATATCG ACGGTTTCAA TGATCCGCTT ATTGACTGTA
     2281 AGAACTGCAA GAGTCGTTGG CGTGCAGATA AGTTAATCGA AGAATTCGAT TCCTCTATCA
     2341 ACACTGGAAT CATGACGTCC GCCCAGCTTG AGGACTATAT TAAGCAAAAT AACATTCTTT
     2401 GCAAAAAGTG CCAGAAAAAG GACTTTACGC AAATCCGCAA GTTTGCTTTG ATGTTCAAAA
     2461 CAAACCAAGG GGTATTAGAA GATGATAGTA GCATTGTATA CTTGCGCCCC GAAACCGCTC
     2521 AAGGCATCTT CATTAACTTT AAGAATGTGC AGCGCTCTAT GCGCAAGAAG CTGCCTTTTG
     2581 GTATTGGGCA AATTGGAAAA AGTTTTCGCA ACGAAATTAC TCCAGGGAAC TTTATCTTCC
     2641 GCACTCGTGA ATTTGAGCAG ATGGAGTTGG AGTTCTTCTT CGATCCGAAC GATCAGCGTG
     2701 ATTGGTTTAA GTATTGGCTG GATCAGATTG AGTTGTTTCT TACAAAGAAG ATCTGTTTGG
     2761 ATAAATCCAA TTACAAAATC CGTGAAAACT TGAAAGACGA GTTAGCTCAT TACGCGTTAA
     2821 AGACGAGTGA CATTGAATTT AATTTCCCAT TTGGATGGGG TGAGCTGTGG GGGATTTCTC
     2881 ACCGCTCTGA CTTCGATTTG AAGGTCCATC AAAATTTGAG TAAGGAGGAT TTAACTGTCC
     2941 TTAAAGAGGA AACCAACCAA AAAGTTCTGG CCAACGTAAT TGAGCCGAGT GTAGGCGTAG
     3001 AACGTCTTAT GCTTGCCATC TTCTGGCAAG CATATACGGA GGAGCAGTTG GAAGAGAATA
     3061 ACTCGCGCAC TGTCTTGAAA TTACCCTACA ACTTGGCCCC ATATCAGGTG GCTATCACTC
     3121 CGCTGTCCAA GCAGCTGAAT CAGAACGCAA ACCAACTGTT CTTGGACTTA TTAAACGATT
     3181 TTGACGCTGT CTATGACGAG ACTGGTAATA TTGGGAAACG CTATCGCCGC CAAGATGCCA
     3241 TCGGGACCCC TTTTGTAATC ACTTATGATT TCCAAACATT GGAAGATCAG AAAGTTACTA
     3301 TTCGTTACCG TGATACTATG AAACAGGAAC GTATCTTAAT CTCGCAATTG AAGGATTTTT
     3361 TAAACAGTCA GTTCAACTGA AGAGCTTAGA GACC
//