LOCUS       Exported     3896 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3896
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1909..1956
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     3784..3800
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      3801..3832
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             1..0
                     /label="MMSYN1_0406"
                     /note="DnaG: DNA primase"
                     /translation="MVYISNEKINEIISQVSIVDVISSYLHLIKKGSNYLAICPFHNDSNPSLTISPEKRIYTCFSCKASGNVINFVKDFEHVEFLTALKTVCDKANISLDEFKNYNQPIKDLEAETIFKLNSEANNIFKTTLFSNLGIQALEYLKSRNITIEQIKKFEIGFASDKTNLVQKLLDKNYNSLDIEKANLGIITNSYTKDYFINRIIFPIKDENNQVIGFSGRSFTKDNEPKYLNTKENKVFKKSHLAYNIASALKISKSLKKIIVLEGFMDVISLSKIDINNTIALMGTSLSNYHLNLFKRHNLDVLLFLDGDDPGIQANIKISHQLLKEKINVLVIDNQTNNDPDELVNNNVEYLKQIINQPIHPVNYLIEKLWNKVDINNPNQIENFIKKVLNFIFDLNNEILVETTINKLVELTKISEQTIKNNLIELKKQSKPNNSFNKNSTTQVFKTNTQTNKVNKQQSKSKPNDFIKKEYINAERRIFISLLISDQFLDKIAANVDKMIHPDIKHATINLINLYNKKIYQGNDINKAFDLLKEYNLTGFDKKQEEIINNSLLTSIKIRESEIDDAFSKLDSYHNDTEISNLKKLLVESKNKTERFQIWNGIDTLKNKKKKR"
     primer_bind     complement(1961..1977)
                     /note="common sequencing primer, one of multiple similar variants"
                     /label="M13 rev"
     rep_origin      complement(2044..2632)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(2803..3663)
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
                     /label="bla"
     promoter        complement(3664..3768)
                     /note="ampicillin resistance gene promoter"
                     /label="bla promoter"
     CDS             complement(1..0)
                     /label="MMSYN1_0406"
                     /note="DnaG: DNA primase"
                     /translation="MVYISNEKINEIISQVSIVDVISSYLHLIKKGSNYLAICPFHNDSNPSLTISPEKRIYTCFSCKASGNVINFVKDFEHVEFLTALKTVCDKANISLDEFKNYNQPIKDLEAETIFKLNSEANNIFKTTLFSNLGIQALEYLKSRNITIEQIKKFEIGFASDKTNLVQKLLDKNYNSLDIEKANLGIITNSYTKDYFINRIIFPIKDENNQVIGFSGRSFTKDNEPKYLNTKENKVFKKSHLAYNIASALKISKSLKKIIVLEGFMDVISLSKIDINNTIALMGTSLSNYHLNLFKRHNLDVLLFLDGDDPGIQANIKISHQLLKEKINVLVIDNQTNNDPDELVNNNVEYLKQIINQPIHPVNYLIEKLWNKVDINNPNQIENFIKKVLNFIFDLNNEILVETTINKLVELTKISEQTIKNNLIELKKQSKPNNSFNKNSTTQVFKTNTQTNKVNKQQSKSKPNDFIKKEYINAERRIFISLLISDQFLDKIAANVDKMIHPDIKHATINLINLYNKKIYQGNDINKAFDLLKEYNLTGFDKKQEEIINNSLLTSIKIRESEIDDAFSKLDSYHNDTEISNLKKLLVESKNKTERFQIWNGIDTLKNKKKKR"
ORIGIN
        1 ATGGTGTACA TCTCAAACGA AAAGATCAAT GAAATCATCA GCCAGGTGAG CATTGTGGAT
       61 GTGATTAGCA GTTACTTGCA CCTTATCAAG AAAGGCTCTA ACTACCTTGC TATCTGTCCG
      121 TTCCACAACG ACAGCAATCC TTCGTTGACT ATTTCTCCCG AGAAGCGTAT TTATACCTGT
      181 TTCTCATGCA AGGCTAGCGG CAACGTTATC AACTTTGTTA AAGACTTTGA ACATGTTGAG
      241 TTTTTGACGG CGTTAAAAAC CGTGTGTGAT AAGGCAAACA TTTCACTGGA TGAATTCAAA
      301 AATTATAATC AGCCTATTAA AGACCTTGAA GCCGAGACAA TCTTCAAACT GAACAGTGAA
      361 GCTAACAACA TTTTCAAGAC AACGTTGTTC TCCAACTTGG GTATCCAGGC ATTAGAGTAT
      421 CTGAAATCGC GTAATATTAC AATCGAACAG ATCAAAAAAT TTGAAATCGG ATTTGCGTCT
      481 GATAAGACGA ACTTAGTTCA AAAGTTGTTG GATAAGAATT ATAACTCTCT TGACATCGAG
      541 AAGGCGAACC TGGGGATCAT CACCAACTCG TATACGAAAG ATTATTTTAT CAACCGCATT
      601 ATTTTTCCGA TCAAAGACGA GAATAACCAG GTAATCGGAT TTTCCGGCCG TTCGTTTACG
      661 AAGGACAACG AGCCGAAATA CTTGAACACC AAGGAGAATA AGGTTTTCAA GAAAAGCCAC
      721 TTAGCATATA ATATTGCAAG TGCACTGAAG ATTTCTAAGT CCTTAAAGAA GATCATCGTG
      781 CTGGAAGGAT TCATGGATGT GATTTCGTTA TCGAAGATTG ACATTAACAA TACTATTGCA
      841 TTAATGGGTA CGTCCCTGTC CAATTATCAC TTGAACTTGT TCAAGCGCCA CAACCTGGAT
      901 GTTTTGTTAT TCTTGGACGG AGATGACCCG GGCATTCAGG CGAATATTAA GATTAGCCAC
      961 CAGTTATTGA AGGAGAAGAT TAACGTACTT GTGATTGACA ACCAGACAAA TAATGACCCG
     1021 GATGAGCTGG TGAACAACAA TGTCGAATAC TTGAAGCAGA TTATCAACCA ACCAATCCAC
     1081 CCAGTTAACT ACCTTATCGA GAAACTTTGG AACAAGGTAG ACATTAACAA CCCAAACCAG
     1141 ATCGAAAATT TTATTAAGAA AGTGTTAAAC TTCATCTTCG ACTTAAACAA CGAAATTTTG
     1201 GTAGAGACTA CGATTAATAA GTTAGTTGAA CTTACGAAAA TCTCTGAACA GACCATTAAA
     1261 AATAATTTGA TCGAGTTAAA GAAGCAGTCC AAACCCAACA ATTCATTTAA TAAAAATAGT
     1321 ACAACACAAG TGTTTAAGAC TAACACACAG ACTAACAAGG TAAATAAACA GCAGAGTAAG
     1381 TCTAAGCCGA ACGATTTCAT TAAGAAAGAG TATATTAACG CTGAGCGTCG TATCTTTATT
     1441 TCTCTTCTGA TTTCAGACCA GTTCCTTGAT AAGATCGCTG CTAATGTAGA CAAAATGATC
     1501 CATCCCGACA TTAAGCACGC TACCATTAAC TTAATCAATC TGTATAATAA GAAAATTTAT
     1561 CAAGGAAATG ATATTAATAA AGCATTCGAC CTGTTGAAGG AGTACAATTT AACAGGCTTC
     1621 GATAAAAAAC AAGAGGAGAT CATCAATAAC AGCTTGCTGA CGAGCATCAA AATTCGTGAG
     1681 TCAGAAATTG ACGACGCTTT TTCAAAATTA GATTCCTACC ATAACGATAC CGAAATCTCG
     1741 AACTTAAAGA AGTTATTAGT TGAATCAAAG AATAAAACAG AACGCTTCCA AATCTGGAAT
     1801 GGGATTGACA CGTTGAAAAA TAAGAAGAAG AAGCGCTGAA GAGCTTAGAG ACCGGTACTA
     1861 GTTCCTGCAG GGCTCGAGGT GTCAATCGTC GGAGCCGCTG AGCAATAACT AGCATAACCC
     1921 CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCATG GTCATAGCTG TTTCCTGAGA
     1981 GCTTGGCAGG TGATGACACA CATTAACAAA TTTCGTGAGG AGTCTCCAGA AGAATGCCAT
     2041 TAATTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG
     2101 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT
     2161 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG
     2221 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG
     2281 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG
     2341 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC
     2401 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG
     2461 GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT
     2521 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG
     2581 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC
     2641 TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT
     2701 GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT
     2761 TAAATCAATC TAAAGTATAT ATGAGTAAAC TTGGTCTGAC AGTTACCAAT GCTTAATCAG
     2821 TGAGGCACCT ATCTCAGCGA TCTGTCTATT TCGTTCATCC ATAGTTGCCT GACTCCCCGT
     2881 CGTGTAGATA ACTACGATAC GGGAGGGCTT ACCATCTGGC CCCAGTGCTG CAATGATACC
     2941 GCGAGAACCA CGCTCACCGG CTCCAGATTT ATCAGCAATA AACCAGCCAG CCGGAAGGGC
     3001 CGAGCGCAGA AGTGGTCCTG CAACTTTATC CGCCTCCATC CAGTCTATTA ATTGTTGCCG
     3061 GGAAGCTAGA GTAAGTAGTT CGCCAGTTAA TAGTTTGCGC AACGTTGTTG CCATTGCTAC
     3121 AGGCATCGTG GTGTCACGCT CGTCGTTTGG TATGGCTTCA TTCAGCTCCG GTTCCCAACG
     3181 ATCAAGGCGA GTTACATGAT CCCCCATGTT GTGCAAAAAA GCGGTTAGCT CCTTCGGTCC
     3241 TCCGATCGTT GTCAGAAGTA AGTTGGCCGC AGTGTTATCA CTCATGGTTA TGGCAGCACT
     3301 GCATAATTCT CTTACTGTCA TGCCATCCGT AAGATGCTTT TCTGTGACTG GTGAGTACTC
     3361 AACCAAGTCA TTCTGAGAAT AGTGTATGCG GCGACCGAGT TGCTCTTGCC CGGCGTCAAT
     3421 ACGGGATAAT ACCGCGCCAC ATAGCAGAAC TTTAAAAGTG CTCATCATTG GAAAACGTTC
     3481 TTCGGGGCGA AAACTCTCAA GGATCTTACC GCTGTTGAGA TCCAGTTCGA TGTAACCCAC
     3541 TCGTGCACCC AACTGATCTT CAGCATCTTT TACTTTCACC AGCGTTTCTG GGTGAGCAAA
     3601 AACAGGAAGG CAAAATGCCG CAAAAAAGGG AATAAGGGCG ACACGGAAAT GTTGAATACT
     3661 CATACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG
     3721 ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CCTGCACCAG
     3781 TCAGTAAAAC GACGGCCAGT AGTCAAAAGC CTCCGACCGG AGGCTTTTGA CTTGGTCAGG
     3841 TGGAGTGAAT TCGCGGCCGC TTCTAGAGGC TCTTCGTAAT TACGAAGAGG TCTCTA
//