LOCUS       Exported     2834 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..2834
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      847..894
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     2722..2738
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      2739..2770
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
                     /label="tonB terminator"
     CDS             1..0
                     /note="Putative FolE"
                     /translation="MLLDNIISYLNQLFNPKKASNWDHVGFQFDYKKLNNINISKVLVCLDLTNDCLEFAISNQIQLIITRHPFIFNELKLEKKNPNKKQMIKKLNKHKILVFSIHTNYDSSIKQNLLEILNKKLKINSFKKYGKDKESNLFYLDQKISVNDLINDLKEVFSLNKIRLNSNINLNSKIKDFYLTSGSGASTMIENMLKNCTFITGEVKWDQWIYANSNNVNLIEIGHYAENHFIDDLKNKLQIKFKDIKIFNYDIKNQFIEK"
                     /label="MMSYN1_0409"
     primer_bind     complement(899..915)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(982..1570)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(1741..2601)
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
                     /label="bla"
     promoter        complement(2602..2706)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
     CDS             complement(1..0)
                     /label="MMSYN1_0409"
                     /note="Putative FolE"
                     /translation="MLLDNIISYLNQLFNPKKASNWDHVGFQFDYKKLNNINISKVLVCLDLTNDCLEFAISNQIQLIITRHPFIFNELKLEKKNPNKKQMIKKLNKHKILVFSIHTNYDSSIKQNLLEILNKKLKINSFKKYGKDKESNLFYLDQKISVNDLINDLKEVFSLNKIRLNSNINLNSKIKDFYLTSGSGASTMIENMLKNCTFITGEVKWDQWIYANSNNVNLIEIGHYAENHFIDDLKNKLQIKFKDIKIFNYDIKNQFIEK"
ORIGIN
        1 ATGCTGTTAG ACAACATTAT TAGCTACTTG AACCAACTTT TCAATCCCAA AAAAGCGTCA
       61 AACTGGGACC ATGTTGGGTT TCAGTTCGAC TACAAAAAAT TGAACAACAT TAACATTTCA
      121 AAAGTACTGG TCTGCCTGGA TCTGACGAAT GATTGCCTGG AATTTGCGAT TAGCAACCAG
      181 ATCCAATTAA TTATCACGCG TCATCCTTTC ATCTTTAATG AGCTGAAGTT GGAGAAGAAA
      241 AATCCGAATA AGAAACAAAT GATCAAAAAA CTGAACAAGC ATAAAATTTT AGTGTTCTCC
      301 ATTCACACTA ATTATGATAG CTCAATTAAG CAGAATTTGC TGGAGATCCT GAATAAGAAG
      361 TTAAAGATTA ACAGTTTCAA AAAGTATGGA AAAGATAAGG AAAGCAACTT ATTCTATCTG
      421 GACCAAAAGA TTAGTGTTAA CGACTTGATC AACGACTTAA AGGAGGTTTT TTCGTTAAAT
      481 AAGATTCGCT TAAATTCCAA CATTAACTTA AACTCAAAAA TCAAAGACTT CTATCTTACC
      541 TCGGGATCAG GGGCCAGTAC CATGATCGAG AATATGTTGA AGAACTGTAC GTTTATCACT
      601 GGTGAAGTCA AATGGGACCA GTGGATCTAC GCAAACTCGA ACAACGTGAA TTTAATCGAA
      661 ATCGGACATT ATGCAGAAAA TCATTTTATC GATGACTTAA AAAATAAACT TCAGATCAAG
      721 TTTAAAGACA TTAAAATTTT CAACTATGAC ATCAAAAACC AATTCATCGA GAAGTGAAGA
      781 GCTTAGAGAC CGGTACTAGT TCCTGCAGGG CTCGAGGTGT CAATCGTCGG AGCCGCTGAG
      841 CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCATGGT
      901 CATAGCTGTT TCCTGAGAGC TTGGCAGGTG ATGACACACA TTAACAAATT TCGTGAGGAG
      961 TCTCCAGAAG AATGCCATTA ATTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA
     1021 TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC
     1081 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC
     1141 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG
     1201 TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA
     1261 CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC
     1321 GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC
     1381 AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG
     1441 CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA
     1501 AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA
     1561 AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA
     1621 CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT
     1681 AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAAACTT GGTCTGACAG
     1741 TTACCAATGC TTAATCAGTG AGGCACCTAT CTCAGCGATC TGTCTATTTC GTTCATCCAT
     1801 AGTTGCCTGA CTCCCCGTCG TGTAGATAAC TACGATACGG GAGGGCTTAC CATCTGGCCC
     1861 CAGTGCTGCA ATGATACCGC GAGAACCACG CTCACCGGCT CCAGATTTAT CAGCAATAAA
     1921 CCAGCCAGCC GGAAGGGCCG AGCGCAGAAG TGGTCCTGCA ACTTTATCCG CCTCCATCCA
     1981 GTCTATTAAT TGTTGCCGGG AAGCTAGAGT AAGTAGTTCG CCAGTTAATA GTTTGCGCAA
     2041 CGTTGTTGCC ATTGCTACAG GCATCGTGGT GTCACGCTCG TCGTTTGGTA TGGCTTCATT
     2101 CAGCTCCGGT TCCCAACGAT CAAGGCGAGT TACATGATCC CCCATGTTGT GCAAAAAAGC
     2161 GGTTAGCTCC TTCGGTCCTC CGATCGTTGT CAGAAGTAAG TTGGCCGCAG TGTTATCACT
     2221 CATGGTTATG GCAGCACTGC ATAATTCTCT TACTGTCATG CCATCCGTAA GATGCTTTTC
     2281 TGTGACTGGT GAGTACTCAA CCAAGTCATT CTGAGAATAG TGTATGCGGC GACCGAGTTG
     2341 CTCTTGCCCG GCGTCAATAC GGGATAATAC CGCGCCACAT AGCAGAACTT TAAAAGTGCT
     2401 CATCATTGGA AAACGTTCTT CGGGGCGAAA ACTCTCAAGG ATCTTACCGC TGTTGAGATC
     2461 CAGTTCGATG TAACCCACTC GTGCACCCAA CTGATCTTCA GCATCTTTTA CTTTCACCAG
     2521 CGTTTCTGGG TGAGCAAAAA CAGGAAGGCA AAATGCCGCA AAAAAGGGAA TAAGGGCGAC
     2581 ACGGAAATGT TGAATACTCA TACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG
     2641 TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT
     2701 TCCGCGCACC TGCACCAGTC AGTAAAACGA CGGCCAGTAG TCAAAAGCCT CCGACCGGAG
     2761 GCTTTTGACT TGGTCAGGTG GAGTGAATTC GCGGCCGCTT CTAGAGGCTC TTCGTAATTA
     2821 CGAAGAGGTC TCTA
//