LOCUS       Exported     6178 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..6178
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      38..85
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
                     /label="T7 terminator"
     primer_bind     1933..1949
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      1950..1981
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             2010..6164
                     /label="MMSYN1_0412"
                     /note=""
                     /translation="MKNRLASFKKVLRFIIVLILILALLTGIGFSSYKISNNISYGAKFVGGYQALVGVYDKTKNQEEEIPNGDAFKGAQSLEKKLSPFSDNTIETQQSGLSRVFIKASKKAYANNQDDFKNAIERTGGLFILDKNYQDIFFNENLMKIIGIDKVYDSSSDKRVAKKLTLEEFLGEAKAESLQPANFSNKNSPFVSFNLKNDYLKNLIDPKKNKDNNALTMITSVGHIIENLRTYYKKANSTNNQVVEQYLNTYFNQIIKPIQDYISSKASSDPLGVKILKDLFTIEYSVPTTEGSQKNLNIQRNSLVDSDIVKWWNSNSKGKIDNTKDLKYVLFGTDNLNNKRSDHIFRFTNSANKYIYDANASEKDFIKDDKGNVGKYYNKLKISSDNSTSQDIELDYIDKVSNSLIKILMTEILFKKDTTDKDYVVNKNLDQNLFRNNILLHNNQISPENIRIVTTNPAIVTDRKTQTTKLYIPVWSNTMAKQVESDILQTSLGFTFKVLSIKEFSADITTIMLIITLACLVILALAVLVFMLFSYRLLGLFAIILAAISASLTMFVPIIFNMAIGPEIFMIMFVGIGLILDASIIYFENLKTHIYKEKLSPESSFKISNKDTLPISLDTSFIILIPSVLLFIFGSGALKNLATISVINILIIILFVILGLRLLTWLVLKAKLFTKYPWLLPLNTIKNSQSTWFNDLMLSFYLSRIENLNTKTKLTTKDLAKLKKFKDKYDFYLNKQEQIITNKKLKQLKKDQHNLELVNKRLLKLENKYEIYLNKQEQINNNKKAKILKKKKSEEIKNKYLLKLENKKQKIINKNSFTKSSIKNSLIKQEFLQARINSNTSSQVLETLENKNKQRRIFKINKIFTIIFIICTFLGAIIGITIGPNYNSSFGKSYSVIAYGQKINDIYDNLDEAIRNYKQFDPSTKRGKDIISMGEHLEKVKNSQDAYMKSKFKVDYSNLTKPSDKNQWASYVVGNIYKEIVKKNYVSLWKNPVSYNKYFRASVDVDYGYDFIDTNSLNLDHKQLAYVGFNIINAQDNKIISIFEKLLVKDNLQNPDLSIKYDHDDNSSANGIINLINVPYTAYGEIKNIAIIFAITLLALLVYILIRFKWTYFVALALTLILVIVLVSSLVVIFRVPVGIEILSAILAVLSFTIITCVLFLGKGKSIIKSKDNKTFTEMFEKEIIAFSNKKATRHQVHEKNHQLKVEYKTNKKNLIKQQIEQNNIKSWWSKIFFKLKQNIKLRFNKKDNSMYKDFKLKIKENKNILKHHKKHTKIEIDLIANNNAFLKETFINVFKFGISRTVLITVIYLAYAIILSFGLYAIIGMGLTIIIGILIASLVSLFIALPIWIWLEKKRMIYVLGYRYYVQNFKINQEEQIINGIND"
     primer_bind     complement(90..106)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(173..761)
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
                     /label="ori"
     CDS             complement(952..1812)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(1813..1917)
                     /note="ampicillin resistance gene promoter"
                     /label="bla promoter"
ORIGIN
        1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC
       61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT
      121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA
      181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC
      241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG
      301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC
      361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG
      421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC
      481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA
      541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG
      601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA
      661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG
      721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA
      781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA
      841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA
      901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG
      961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG
     1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC
     1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC
     1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA
     1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC
     1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG
     1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC
     1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT
     1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG
     1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC
     1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG
     1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT
     1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG
     1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG
     1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT
     1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC
     1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC
     1981 TTGGTTCAGG TGGAGTGGGA GGGTCTCAAA TGAAAAACCG CTTGGCGTCC TTTAAGAAAG
     2041 TATTGCGTTT CATTATTGTT TTAATTCTGA TTCTTGCGTT ACTGACAGGC ATTGGGTTCA
     2101 GTTCTTACAA AATCTCTAAT AACATTTCGT ATGGGGCGAA ATTTGTTGGC GGCTACCAGG
     2161 CGCTGGTAGG CGTTTACGAT AAAACAAAGA ATCAAGAAGA AGAGATTCCA AACGGCGACG
     2221 CGTTCAAAGG GGCACAGTCC CTTGAAAAGA AGCTGTCACC ATTCTCAGAT AATACAATTG
     2281 AGACACAGCA GTCAGGGTTG TCCCGCGTAT TCATCAAGGC GAGCAAAAAG GCTTATGCTA
     2341 ATAACCAAGA TGACTTCAAA AATGCCATCG AACGTACGGG CGGGTTATTC ATCTTGGACA
     2401 AGAACTACCA GGATATATTT TTTAATGAGA ACTTAATGAA GATCATTGGG ATTGACAAAG
     2461 TCTATGACTC TTCCTCTGAT AAGCGCGTCG CAAAGAAATT AACGTTGGAA GAGTTTTTGG
     2521 GGGAGGCGAA GGCGGAGAGT TTACAGCCTG CAAACTTTAG CAACAAGAAC TCCCCTTTTG
     2581 TATCGTTCAA TCTGAAGAAC GACTACCTGA AGAACCTTAT CGACCCGAAA AAGAATAAAG
     2641 ATAATAACGC TCTGACCATG ATTACCTCTG TGGGACATAT CATCGAAAAT CTGCGTACGT
     2701 ACTACAAGAA GGCCAACAGT ACTAACAATC AGGTCGTGGA ACAGTACCTG AACACATACT
     2761 TTAACCAAAT CATCAAACCT ATCCAAGATT ACATTTCCTC GAAAGCCTCG AGTGATCCCT
     2821 TGGGGGTCAA AATTTTAAAG GACCTTTTTA CGATCGAATA CTCAGTCCCC ACAACTGAAG
     2881 GGAGTCAGAA AAATTTAAAT ATCCAGCGTA ATTCACTTGT GGATTCGGAT ATTGTTAAGT
     2941 GGTGGAACTC TAATAGCAAG GGGAAGATCG ATAACACAAA AGACCTTAAA TACGTCCTGT
     3001 TCGGAACTGA CAATCTTAAT AACAAACGCT CGGACCATAT TTTTCGCTTT ACAAACTCTG
     3061 CAAATAAGTA TATTTATGAC GCAAACGCAT CCGAGAAGGA TTTCATTAAA GATGATAAGG
     3121 GCAATGTTGG GAAATACTAT AATAAGTTGA AAATTTCGTC CGATAATTCC ACGAGTCAGG
     3181 ATATTGAGCT TGATTACATC GACAAAGTGT CGAACAGTTT AATCAAAATT CTGATGACGG
     3241 AAATCTTGTT CAAAAAGGAT ACAACAGATA AAGACTACGT AGTCAATAAG AATTTGGACC
     3301 AGAACTTATT CCGCAACAAT ATCCTGTTAC ATAATAATCA GATTTCACCC GAAAATATCC
     3361 GCATTGTGAC AACAAATCCA GCAATCGTGA CGGACCGTAA GACGCAGACG ACAAAGCTTT
     3421 ACATCCCCGT TTGGTCGAAC ACCATGGCCA AGCAAGTCGA GTCCGACATC TTGCAAACTT
     3481 CACTGGGTTT CACCTTTAAA GTCCTTTCCA TCAAAGAGTT TTCTGCAGAT ATCACAACTA
     3541 TTATGCTTAT TATTACATTA GCATGTTTAG TGATCCTTGC TTTAGCAGTG CTTGTTTTCA
     3601 TGTTATTTTC CTATCGTCTT TTGGGCTTAT TCGCCATCAT TTTAGCTGCC ATCAGTGCCT
     3661 CCCTTACAAT GTTCGTGCCG ATCATCTTCA ACATGGCCAT TGGACCCGAA ATCTTTATGA
     3721 TCATGTTTGT TGGAATCGGT TTGATTTTAG ATGCGTCGAT CATCTATTTC GAGAACTTAA
     3781 AGACACACAT TTATAAAGAA AAGTTATCAC CCGAGTCCTC CTTTAAAATT TCAAATAAAG
     3841 ACACGTTACC TATCTCGCTG GATACCAGTT TTATCATCCT TATTCCCAGC GTGTTATTAT
     3901 TCATCTTTGG GAGTGGGGCC TTAAAGAATC TTGCGACTAT TAGTGTGATT AACATCCTTA
     3961 TTATCATTCT TTTTGTGATC CTGGGGTTAC GCTTACTGAC TTGGCTTGTG TTGAAGGCTA
     4021 AGTTGTTCAC TAAGTATCCC TGGCTGCTTC CTTTGAACAC GATTAAGAAC TCGCAATCAA
     4081 CCTGGTTCAA CGACTTAATG CTGTCTTTCT ACCTTTCTCG TATCGAGAAC CTTAACACCA
     4141 AAACCAAGCT TACAACGAAG GACTTAGCCA AGCTTAAGAA ATTCAAGGAT AAATACGATT
     4201 TCTACCTTAA TAAGCAAGAG CAGATCATCA CAAATAAAAA GCTGAAGCAG TTGAAGAAAG
     4261 ATCAACACAA CCTTGAACTG GTTAACAAAC GCTTGCTTAA GTTAGAGAAC AAATATGAAA
     4321 TTTACCTTAA TAAGCAGGAG CAGATTAATA ACAATAAAAA GGCTAAAATT TTAAAGAAGA
     4381 AAAAGTCGGA AGAAATTAAG AACAAATATT TACTTAAGCT GGAGAATAAG AAGCAAAAGA
     4441 TTATCAACAA AAATTCTTTC ACGAAGTCGT CTATCAAGAA CTCTCTTATT AAGCAAGAAT
     4501 TCCTTCAGGC TCGCATTAAC TCCAATACCT CGTCGCAAGT CCTTGAGACT CTGGAAAACA
     4561 AGAACAAACA ACGTCGTATT TTTAAAATTA ATAAAATCTT CACGATTATC TTTATCATTT
     4621 GTACTTTCCT TGGGGCCATT ATCGGCATTA CCATTGGTCC TAATTATAAT TCATCATTCG
     4681 GCAAGAGTTA CTCGGTTATT GCTTATGGAC AGAAGATTAA CGACATTTAC GATAATCTGG
     4741 ATGAAGCCAT TCGTAATTAC AAGCAATTTG ACCCGTCTAC AAAACGTGGC AAGGACATCA
     4801 TTTCAATGGG AGAGCACCTT GAAAAAGTCA AGAACAGTCA GGACGCTTAT ATGAAGTCTA
     4861 AGTTCAAAGT TGACTATTCT AACCTGACAA AGCCAAGTGA TAAAAATCAA TGGGCCTCGT
     4921 ACGTCGTGGG TAACATTTAC AAGGAAATTG TTAAGAAAAA TTATGTTTCA TTATGGAAGA
     4981 ATCCAGTGAG TTACAACAAG TATTTCCGCG CGTCTGTAGA CGTGGACTAT GGATACGACT
     5041 TCATTGATAC TAACTCATTA AATCTGGACC ATAAACAACT TGCCTACGTT GGATTCAACA
     5101 TTATTAATGC GCAGGACAAT AAGATCATTT CCATCTTTGA AAAACTTTTG GTTAAAGATA
     5161 ATCTGCAGAA CCCCGACCTT AGCATTAAAT ACGACCACGA CGATAACTCA TCCGCAAATG
     5221 GAATCATCAA TCTTATTAAT GTCCCATATA CCGCGTATGG TGAAATTAAG AATATTGCGA
     5281 TCATCTTTGC CATCACCCTT TTGGCTTTGT TAGTCTACAT TTTGATCCGC TTTAAATGGA
     5341 CCTATTTTGT CGCGTTAGCC TTAACGCTTA TCCTTGTAAT TGTGCTTGTT AGTTCATTGG
     5401 TCGTGATCTT TCGTGTTCCT GTGGGAATTG AAATTCTGTC TGCGATTTTA GCAGTTCTGT
     5461 CTTTTACCAT TATCACTTGT GTGTTATTCT TGGGAAAAGG GAAGTCCATT ATTAAGAGTA
     5521 AAGATAATAA GACTTTTACT GAAATGTTCG AGAAGGAAAT TATCGCCTTC TCTAACAAAA
     5581 AGGCGACTCG TCACCAAGTT CATGAGAAAA ATCATCAATT GAAAGTTGAG TATAAAACTA
     5641 ACAAGAAGAA TTTAATCAAA CAACAAATTG AGCAAAACAA TATTAAATCT TGGTGGAGCA
     5701 AAATCTTTTT TAAGTTGAAG CAAAACATTA AGTTGCGTTT CAACAAGAAA GACAATTCCA
     5761 TGTACAAAGA CTTTAAACTT AAAATCAAAG AGAATAAAAA CATCCTTAAG CACCATAAGA
     5821 AACACACTAA GATCGAAATT GATTTAATTG CTAATAACAA CGCTTTTCTT AAAGAAACGT
     5881 TCATTAATGT GTTCAAGTTC GGTATTAGCC GCACGGTACT TATCACGGTG ATCTATCTGG
     5941 CGTACGCTAT TATTCTTAGT TTCGGTCTGT ATGCCATCAT CGGCATGGGC TTAACAATCA
     6001 TCATCGGTAT TCTGATTGCT TCACTTGTCT CACTTTTTAT CGCGCTGCCT ATTTGGATCT
     6061 GGTTGGAGAA AAAGCGTATG ATTTACGTAT TAGGATATCG CTATTATGTG CAAAACTTCA
     6121 AGATTAACCA AGAGGAACAG ATTATCAACG GTATCAACGA TTGAAGAGCT TAGAGACC
//