LOCUS Exported 2570 bp DNA circular SYN 24-JUL-2020 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2001) AUTHORS Keoni Gandall TITLE Direct Submission JOURNAL Exported from Poly. FEATURES Location/Qualifiers terminator 1..2570 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" terminator 583..630 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" primer_bind 2458..2474 /note="common sequencing primer, one of multiple similar variants" /label="M13 for" terminator 2475..2506 /label="tonB terminator" /note="bidirectional E. coli tonB-P14 transcription terminator" CDS 1..0 /label="MMSYN1_0413" /note="Catalyzes a salvage reaction resulting in the formation of AMP, that is energically less costly than de novo synthesis." /translation="MNLKEFVVDVKDFPKQGIVFKDITPLLNNKDAFKYTIDQMADFVKKLDVDVVVAPEARGFLLASAVAYAANKRFVLVRKPNKLPREVYDVEYSLEYGTNHQQIHVGDLKPNDKVVVIDDVLATGGTMQAIIDLVKLSKAEVIGMSFLIDLTFLHDVNLFDQYKVQKLIKY" primer_bind complement(635..651) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" rep_origin complement(718..1306) /label="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(1477..2337) /label="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*" promoter complement(2338..2442) /label="bla promoter" /note="ampicillin resistance gene promoter" CDS complement(1..0) /label="MMSYN1_0413" /note="Catalyzes a salvage reaction resulting in the formation of AMP, that is energically less costly than de novo synthesis." /translation="MNLKEFVVDVKDFPKQGIVFKDITPLLNNKDAFKYTIDQMADFVKKLDVDVVVAPEARGFLLASAVAYAANKRFVLVRKPNKLPREVYDVEYSLEYGTNHQQIHVGDLKPNDKVVVIDDVLATGGTMQAIIDLVKLSKAEVIGMSFLIDLTFLHDVNLFDQYKVQKLIKY" ORIGIN 1 ATGAATTTAA AAGAGTTTGT GGTTGACGTA AAAGATTTTC CAAAGCAAGG GATCGTTTTC 61 AAAGATATTA CGCCTTTGCT GAACAACAAG GACGCTTTTA AATATACCAT TGACCAAATG 121 GCTGATTTCG TCAAGAAGCT GGATGTTGAT GTGGTGGTCG CGCCCGAAGC CCGTGGCTTC 181 CTTCTTGCCT CCGCTGTGGC TTACGCCGCT AATAAACGCT TCGTACTTGT TCGTAAGCCT 241 AACAAGCTGC CCCGTGAGGT TTACGATGTG GAGTACAGTT TGGAGTATGG CACCAATCAC 301 CAGCAGATCC ATGTCGGGGA CCTTAAACCG AATGATAAAG TAGTCGTGAT CGATGATGTG 361 CTTGCTACTG GTGGCACCAT GCAAGCAATC ATCGATTTAG TGAAATTATC CAAGGCGGAG 421 GTAATTGGAA TGTCGTTTTT AATCGATCTT ACCTTCCTTC ATGACGTGAA TTTGTTTGAT 481 CAGTATAAAG TCCAGAAACT GATTAAGTAC TGAAGAGCTT AGAGACCGGT ACTAGTTCCT 541 GCAGGGCTCG AGGTGTCAAT CGTCGGAGCC GCTGAGCAAT AACTAGCATA ACCCCTTGGG 601 GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CATGGTCATA GCTGTTTCCT GAGAGCTTGG 661 CAGGTGATGA CACACATTAA CAAATTTCGT GAGGAGTCTC CAGAAGAATG CCATTAATTT 721 CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG 781 AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC 841 TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT 901 GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA 961 GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA 1021 TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA 1081 CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA 1141 CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT 1201 CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT 1261 TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT 1321 CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT 1381 GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC 1441 AATCTAAAGT ATATATGAGT AAACTTGGTC TGACAGTTAC CAATGCTTAA TCAGTGAGGC 1501 ACCTATCTCA GCGATCTGTC TATTTCGTTC ATCCATAGTT GCCTGACTCC CCGTCGTGTA 1561 GATAACTACG ATACGGGAGG GCTTACCATC TGGCCCCAGT GCTGCAATGA TACCGCGAGA 1621 ACCACGCTCA CCGGCTCCAG ATTTATCAGC AATAAACCAG CCAGCCGGAA GGGCCGAGCG 1681 CAGAAGTGGT CCTGCAACTT TATCCGCCTC CATCCAGTCT ATTAATTGTT GCCGGGAAGC 1741 TAGAGTAAGT AGTTCGCCAG TTAATAGTTT GCGCAACGTT GTTGCCATTG CTACAGGCAT 1801 CGTGGTGTCA CGCTCGTCGT TTGGTATGGC TTCATTCAGC TCCGGTTCCC AACGATCAAG 1861 GCGAGTTACA TGATCCCCCA TGTTGTGCAA AAAAGCGGTT AGCTCCTTCG GTCCTCCGAT 1921 CGTTGTCAGA AGTAAGTTGG CCGCAGTGTT ATCACTCATG GTTATGGCAG CACTGCATAA 1981 TTCTCTTACT GTCATGCCAT CCGTAAGATG CTTTTCTGTG ACTGGTGAGT ACTCAACCAA 2041 GTCATTCTGA GAATAGTGTA TGCGGCGACC GAGTTGCTCT TGCCCGGCGT CAATACGGGA 2101 TAATACCGCG CCACATAGCA GAACTTTAAA AGTGCTCATC ATTGGAAAAC GTTCTTCGGG 2161 GCGAAAACTC TCAAGGATCT TACCGCTGTT GAGATCCAGT TCGATGTAAC CCACTCGTGC 2221 ACCCAACTGA TCTTCAGCAT CTTTTACTTT CACCAGCGTT TCTGGGTGAG CAAAAACAGG 2281 AAGGCAAAAT GCCGCAAAAA AGGGAATAAG GGCGACACGG AAATGTTGAA TACTCATACT 2341 CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT 2401 ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACCTGCA CCAGTCAGTA 2461 AAACGACGGC CAGTAGTCAA AAGCCTCCGA CCGGAGGCTT TTGACTTGGT CAGGTGGAGT 2521 GAATTCGCGG CCGCTTCTAG AGGCTCTTCG TAATTACGAA GAGGTCTCTA //