LOCUS       BBF10K_000195           6306 bp    DNA     circular UNK 27-MAY-2018
DEFINITION  Yeast cds LYS2 in pOpen-v3.
ACCESSION   BBF10K_000195
VERSION     BBF10K_000195.1
KEYWORDS    BBFD_0017; Open Yeast Collection Part; OpenMTA; FreeGenes; Golden
            Gate Assembly.
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
            .
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
REFERENCE   2  (bases 2002 to 6306)
  AUTHORS   Scott Pownall, Open Science Network
  TITLE     Direct Submission
  JOURNAL   https://biobricks.org/freegenes/
FEATURES             Location/Qualifiers
     source          1..2001
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
                     /original_record="pOpen_v3.1"
     primer_bind     complement(24..42)
                     /label="T7 Reverse"
                     /note="/ApEinfo_fwdcolor=#b7e6d7 /ApEinfo_revcolor=#b7e6d7"
                     /original_record="pOpen_v3.1"
     terminator      38..85
                     /label="T7 terminator"
                     /note="/ApEinfo_fwdcolor=#d6b295 /ApEinfo_revcolor=#d6b295"
                     /original_record="pOpen_v3.1"
     primer_bind     complement(86..106)
                     /label="M13 rev"
                     /note="/ApEinfo_fwdcolor=#ff9ccd /ApEinfo_revcolor=#ff9ccd"
                     /original_record="pOpen_v3.1"
     misc_feature    107..121
                     /label="AarI"
                     /note="/ApEinfo_fwdcolor=#b1ff67 /ApEinfo_revcolor=#b1ff67"
                     /original_record="pOpen_v3.1"
     misc_feature    122..172
                     /label="spacer"
                     /note="/ApEinfo_fwdcolor=#b1ff67 /ApEinfo_revcolor=#b1ff67"
                     /original_record="pOpen_v3.1"
     rep_origin      complement(173..761)
                     /direction=LEFT
                     /label="ori"
                     /note="/ApEinfo_fwdcolor=#84b0dc /ApEinfo_revcolor=#84b0dc"
                     /original_record="pOpen_v3.1"
     CDS             complement(952..1812)
                     /label="AmpR"
                     /note="/ApEinfo_fwdcolor=#ff9ccd /ApEinfo_revcolor=#ff9ccd"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW*"
                     /original_record="pOpen_v3.1"
     misc_feature    1918..1932
                     /label="AarI"
                     /note="/ApEinfo_fwdcolor=#d6b295 /ApEinfo_revcolor=#d6b295"
                     /original_record="pOpen_v3.1"
     primer_bind     1933..1949
                     /label="M13 fwd"
                     /note="/ApEinfo_fwdcolor=#84b0dc /ApEinfo_revcolor=#84b0dc"
                     /original_record="pOpen_v3.1"
     terminator      1950..1981
                     /label="tonB terminator"
                     /note="/ApEinfo_fwdcolor=#b4abac /ApEinfo_revcolor=#b4abac"
                     /original_record="pOpen_v3.1"
     CDS             2030..6208
                     /codon_start=1
                     /gene="LYS2"
                     /product="LYS2 selectable marker"
                     /label="LYS2"
                     /translation="MTNEKVWIEKLDNPTLSVLPHDFLRPQQEPYTKQATYSLQLPQLD
                     VPHDSFSNKYAVALSVWAALIYRVTGDDDIVLYIANNKILRFNIQPTWSFNELYSTINN
                     ELNKLNSIEANFSFDELAEKIQSCQDLERTPQLFRLAFLENQDFKLDEFKHHLVDFALN
                     LDTSNNAHVLNLIYNSLLYSNERVTIVADQFTQYLTAALSDPSNCITKISLITASSKDS
                     LPDPTKNLGWCDFVGCIHDIFQDNAEAFPERTCVVETPTLNSDKSRSFTYRDINRTSNI
                     VAHYLIKTGIKRGDVVMIYSSRGVDLMVCVMGVLKAGATFSVIDPAYPPARQTIYLGVA
                     KPRGLIVIRAAGQLDQLVEDYINDELEIVSRINSIAIQENGTIEGGKLDNGEDVLAPYD
                     HYKDTRTGVVVGPDSNPTLSFTSGSEGIPKGVLGRHFSLAYYFNWMSKRFNLTENDKFT
                     MLSGIAHDPIQRDMFTPLFLGAQLYVPTQDDIGTPGRLAEWMSKYGCTVTHLTPAMGQL
                     LTAQATTPFPKLHHAFFVGDILTKRDCLRLQTLAENCRIVNMYGTTETQRAVSYFEVKS
                     KNDDPNFLKKLKDVMPAGKGMLNVQLLVVNRNDRTQICGIGEIGEIYVRAGGLAEGYRG
                     LPELNKEKFVNNWFVEKDHWNYLDKDNGEPWRQFWLGPRDRLYRTGDLGRYLPNGDCEC
                     CGRADDQVKIRGFRIELGEIDTHISQHPLVRENITLVRKNADNEPTLITFMVPRFDKPD
                     DLSKFQSDVPKEVETDPIVKGLIGYHLLSKDIRTFLKKRLASYAMPSLIVVMDKLPLNP
                     NGKVDKPKLQFPTPKQLNLVAENTVSETDDSQFTNVEREVRDLWLSILPTKPASVSPDD
                     SFFDLGGHSILATKMIFTLKKKLQVDLPLGTIFKYPTIKAFAAEIDRIKSSGGSSQGEV
                     VENVTANYAEDAKKLVETLPSSYPSREYFVEPNSAEGKTTINVFVTGVTGFLGSYILAD
                     LLGRSPKNYSFKVFAHVRAKDEEAAFARLQKAGITYGTWNEKFASNIKVVLGDLSKSQF
                     GLSDEKWMDLANTVDIIIHNGALVHWVYPYAKLRDPNVISTINVMSLAAVGKPKFFDFV
                     SSTSTLDTEYYFNLSDKLVSEGKPGILESDDLMNSASGLTGGYGQSKWAAEYIIRRAGE
                     RGLRGCIVRPGYVTGASANGSSNTDDFLLRFLKGSVQLGKIPDIENSVNMVPVDHVARV
                     VVATSLNPPKENELAVAQVTGHPRILFKDYLYTLHDYGYDVEIESYSKWKKSLEASVID
                     RNEENALYPLLHMVLDNLPESTKAPELDDRNAVASLKKDTAWTGVDWSNGIGVTPEEVG
                     IYIAFLNKVGFLPPPTHNDKLPLPSIELTQAQISLVASGAGARGSSAAA"
                     /original_record="part"
     protein_bind    complement(join(6203..6205,6207..6213))
                     /label="SapI"
                     /ApEinfo_fwdcolor="#00FF2C"
                     /ApEinfo_revcolor="#00FF2C"
                     /original_record="part"
     protein_bind    6214..6219
                     /label="HindIII"
                     /ApEinfo_fwdcolor="#00FF2C"
                     /ApEinfo_revcolor="#00FF2C"
                     /original_record="part"
     misc_feature    2002..2021
                     /label="FreeGenes dialout primer"
                     /ApEinfo_fwdcolor="#0048BA"
                     /ApEinfo_revcolor="#0048BA"
     misc_feature    complement(6227..6246)
                     /label="FreeGenes dialout primer"
                     /ApEinfo_fwdcolor="#0048BA"
                     /ApEinfo_revcolor="#0048BA"
     misc_feature    6247..6306
                     /label="FreeGenes barcode"
                     /ApEinfo_fwdcolor="#FF7E00"
                     /ApEinfo_revcolor="#FF7E00"
     protein_bind    join(2022..2027,2029..2032)
                     /label="BsaI"
                     /ApEinfo_fwdcolor="#00FF2C"
                     /ApEinfo_revcolor="#00FF2C"
     protein_bind    complement(join(6216..6219,6221..6226))
                     /label="BsaI"
                     /ApEinfo_fwdcolor="#00FF2C"
                     /ApEinfo_revcolor="#00FF2C"
     protein_bind    6268..6285
                     /label="I-SceI"
                     /ApEinfo_fwdcolor="#4F0080"
                     /ApEinfo_revcolor="#4F0080"
ORIGIN
        1 cgctgaggtg tcaatcgtcg gagccgctga gcaataacta gcataacccc ttggggcctc
       61 taaacgggtc ttgaggggtt ttttgcatgg tcatagctgt ttcctgagag cttggcaggt
      121 gatgacacac attaacaaat ttcgtgagga gtctccagaa gaatgccatt aatttccata
      181 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
      241 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
      301 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
      361 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
      421 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
      481 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
      541 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
      601 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
      661 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
      721 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaaggccta ctattagcaa
      781 caacgatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
      841 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
      901 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
      961 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     1021 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     1081 aatgataccg cgagaaccac gctcaccggc tccagattta tcagcaataa accagccagc
     1141 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     1201 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     1261 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     1321 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     1381 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     1441 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     1501 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     1561 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     1621 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     1681 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     1741 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     1801 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     1861 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     1921 ctgcaccagt cagtaaaacg acggccagta gtcaaaagcc tccgaccgga ggcttttgac
     1981 ttggttcagg tggagtggga gaacgatgat gctcactctc gggtctctaa tgactaacga
     2041 aaaggtctgg atagagaagt tggataatcc aactctttca gtgttaccac atgacttttt
     2101 acgcccacaa caagaacctt atacgaaaca agctacatat tcgttacagc tacctcagct
     2161 cgatgtgcct catgatagtt tttctaacaa atacgctgtc gctttgagtg tatgggctgc
     2221 attgatatat agagtaaccg gtgacgatga tattgttctt tatattgcga ataacaaaat
     2281 cttaagattc aatattcaac caacgtggtc atttaatgag ctgtattcta caattaacaa
     2341 tgagttgaac aagctcaatt ctattgaggc caatttttcc tttgacgaac tagctgaaaa
     2401 aattcaaagt tgccaagacc tggaaaggac ccctcagttg ttccgtttgg cctttttgga
     2461 aaaccaagat ttcaaattag acgagttcaa gcatcattta gtggactttg ctttgaattt
     2521 ggataccagt aataatgcgc atgttttgaa cttaatttat aacagcttac tgtattcgaa
     2581 tgaaagagta accattgttg cggaccaatt tactcaatat ttgactgctg cgctaagcga
     2641 tccatccaat tgcataacta aaatctctct gatcaccgca tcatccaagg atagtttacc
     2701 tgatccaact aagaacttgg gctggtgcga tttcgtgggg tgtattcacg acattttcca
     2761 ggacaatgct gaagccttcc cagagagaac ctgtgttgtg gagactccaa cactaaattc
     2821 cgacaagtcc cgttctttca cttatcgtga catcaaccgc acttctaaca tagttgccca
     2881 ttatttgatt aaaacaggta tcaaaagagg tgatgtagtg atgatctatt cttctagggg
     2941 tgtggatttg atggtatgtg tgatgggtgt cttgaaagcc ggcgcaacct tttcagttat
     3001 cgaccctgca tatcccccag ccagacaaac catttactta ggtgttgcta aaccacgtgg
     3061 gttgattgtt attagagctg ctggacaatt ggatcaactg gtagaagatt acatcaatga
     3121 tgaattggag attgtttcaa gaatcaattc cattgctatt caagaaaatg gtaccattga
     3181 aggtggcaaa ttggacaatg gcgaggatgt tttggctcca tatgatcact acaaagacac
     3241 cagaacaggt gttgtagttg gaccagattc caacccaacc ctatctttca catctggttc
     3301 cgaaggtatt cctaagggtg ttcttggtag acatttttcc ttggcttatt atttcaattg
     3361 gatgtccaaa aggttcaact taacagaaaa tgataaattc acaatgctga gcggtattgc
     3421 acatgatcca attcaaagag atatgtttac accattattt ttaggtgccc aattgtatgt
     3481 ccctactcaa gatgatattg gtacaccggg ccgtttagcg gaatggatga gtaagtatgg
     3541 ttgcacagtt acccatttaa caccagccat gggtcaatta cttactgccc aagctactac
     3601 accattccct aagttacatc atgcgttctt tgtgggtgac attttaacaa aacgtgattg
     3661 tctgaggtta caaaccttgg cagaaaattg ccgtattgtt aatatgtacg gtaccactga
     3721 aacacagcgt gcagtttctt atttcgaagt taaatcaaaa aatgacgatc caaacttttt
     3781 gaaaaaattg aaagatgtca tgcctgctgg taaaggtatg ttgaacgttc agctgctagt
     3841 tgttaacagg aacgatcgta ctcaaatatg tggtattggc gaaataggtg agatttatgt
     3901 tcgtgcaggc ggtttggccg aaggttatag aggattacca gaattgaata aagaaaaatt
     3961 tgtgaacaac tggtttgttg aaaaagatca ctggaattat ttggataagg ataatggtga
     4021 accttggaga caattctggt taggtccaag agatagattg tacagaacgg gtgatttagg
     4081 tcgttatcta ccaaacggtg actgtgaatg ttgcggtagg gctgatgatc aagttaaaat
     4141 tcgtgggttc agaatcgaat taggagaaat agatacgcac atttcccaac atccattggt
     4201 aagagaaaac attactttag ttcgcaaaaa tgccgacaat gagccaacat tgatcacatt
     4261 tatggtccca agatttgaca agccagatga cttgtctaag ttccaaagtg atgttccaaa
     4321 ggaggttgaa actgacccta tagttaaggg cttaatcggt taccatcttt tatccaagga
     4381 catcaggact ttcttaaaga aaagattggc tagttatgct atgccttcct tgattgtggt
     4441 tatggataaa ctaccattga atccaaatgg taaagttgat aagcctaaac ttcaattccc
     4501 aactcccaag caattaaatt tggtagctga aaatacagtt tctgaaactg acgactctca
     4561 gtttaccaat gttgagcgcg aggttagaga cttatggtta agtatattac ctaccaagcc
     4621 agcatctgta tcaccagatg attcgttttt cgatttaggt ggtcattcta tcttggctac
     4681 caaaatgatt tttaccttaa agaaaaagct gcaagttgat ttaccattgg gcacaatttt
     4741 caagtatcca acgataaagg cctttgccgc ggaaattgac agaattaaat catcgggtgg
     4801 atcatctcaa ggtgaggtcg tcgaaaatgt cactgcaaat tatgcggagg acgccaagaa
     4861 attggttgaa acgctaccaa gttcgtaccc cagtagggaa tattttgttg aacctaatag
     4921 tgccgaagga aaaacaacaa ttaatgtgtt tgttaccggt gtcacaggat ttctgggctc
     4981 ctacatcctt gcagatttgt taggacgttc tccaaagaac tacagtttca aagtgtttgc
     5041 ccacgtcagg gccaaggatg aagaagctgc atttgcaaga ttacaaaagg caggtatcac
     5101 ctatggtact tggaacgaaa aatttgcctc aaatattaaa gttgtattag gcgatttatc
     5161 taaaagccaa tttggtcttt cagatgagaa gtggatggat ttggcaaaca cagttgatat
     5221 aattatccat aatggtgcgt tagttcactg ggtttatcca tatgccaaat tgagggaccc
     5281 aaatgttatt tcaactatca atgttatgag cttagccgcc gtcggcaagc caaagttctt
     5341 tgactttgtt tcctccactt ctactcttga cactgaatac tactttaatt tgtcagataa
     5401 acttgttagc gaagggaagc caggcatttt agaatcagac gatttaatga actctgcaag
     5461 cgggctcact ggtggatatg gtcagtccaa atgggctgct gagtacatca ttagacgtgc
     5521 cggtgaaagg ggcctacgtg ggtgtattgt cagaccaggt tacgtaacag gtgcctctgc
     5581 caatggttct tcaaacacag atgatttctt attgagattt ttgaaaggtt cagtccaatt
     5641 aggtaagatt ccagatatcg aaaattccgt gaatatggtt ccagtagatc atgttgctcg
     5701 tgttgttgtt gctacgtctt tgaatcctcc caaagaaaat gaattggccg ttgctcaagt
     5761 aacgggtcac ccaagaatat tattcaaaga ctacttgtat actttacacg attatggtta
     5821 cgatgtcgaa atcgaaagct attctaaatg gaagaaatca ttggaggcgt ctgttattga
     5881 caggaatgaa gaaaatgcgt tgtatccttt gctacacatg gtcttagaca acttacctga
     5941 aagtaccaaa gctccggaac tagacgatag gaacgccgtg gcatctttaa agaaagacac
     6001 cgcatggaca ggtgttgatt ggtctaatgg aataggtgtt actccagaag aggttggtat
     6061 atatattgca tttttaaaca aggttggatt tttacctcca ccaactcata atgacaaact
     6121 tccactgcca agtatagaac taactcaagc gcaaataagt ctagttgctt caggtgctgg
     6181 tgctcgtgga agctccgcag cagcttgaag agcaagcttt gagaccagat agtcacgcac
     6241 aagaccatct ctaagtgata aaaactttag ggataacagg gtaatgtgaa tggccaatgt
     6301 gaaaga
//
