LOCUS BBF10K_000195 6306 bp DNA circular UNK 27-MAY-2018 DEFINITION Yeast cds LYS2 in pOpen-v3. ACCESSION BBF10K_000195 VERSION BBF10K_000195.1 KEYWORDS BBFD_0017; Open Yeast Collection Part; OpenMTA; FreeGenes; Golden Gate Assembly. SOURCE synthetic DNA construct ORGANISM recombinant plasmid . REFERENCE 1 (bases 1 to 2001) AUTHORS Keoni Gandall TITLE Direct Submission REFERENCE 2 (bases 2002 to 6306) AUTHORS Scott Pownall, Open Science Network TITLE Direct Submission JOURNAL https://biobricks.org/freegenes/ FEATURES Location/Qualifiers source 1..2001 /organism="recombinant plasmid" /mol_type="other DNA" /original_record="pOpen_v3.1" primer_bind complement(24..42) /label="T7 Reverse" /note="/ApEinfo_fwdcolor=#b7e6d7 /ApEinfo_revcolor=#b7e6d7" /original_record="pOpen_v3.1" terminator 38..85 /label="T7 terminator" /note="/ApEinfo_fwdcolor=#d6b295 /ApEinfo_revcolor=#d6b295" /original_record="pOpen_v3.1" primer_bind complement(86..106) /label="M13 rev" /note="/ApEinfo_fwdcolor=#ff9ccd /ApEinfo_revcolor=#ff9ccd" /original_record="pOpen_v3.1" misc_feature 107..121 /label="AarI" /note="/ApEinfo_fwdcolor=#b1ff67 /ApEinfo_revcolor=#b1ff67" /original_record="pOpen_v3.1" misc_feature 122..172 /label="spacer" /note="/ApEinfo_fwdcolor=#b1ff67 /ApEinfo_revcolor=#b1ff67" /original_record="pOpen_v3.1" rep_origin complement(173..761) /direction=LEFT /label="ori" /note="/ApEinfo_fwdcolor=#84b0dc /ApEinfo_revcolor=#84b0dc" /original_record="pOpen_v3.1" CDS complement(952..1812) /label="AmpR" /note="/ApEinfo_fwdcolor=#ff9ccd /ApEinfo_revcolor=#ff9ccd" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW*" /original_record="pOpen_v3.1" misc_feature 1918..1932 /label="AarI" /note="/ApEinfo_fwdcolor=#d6b295 /ApEinfo_revcolor=#d6b295" /original_record="pOpen_v3.1" primer_bind 1933..1949 /label="M13 fwd" /note="/ApEinfo_fwdcolor=#84b0dc /ApEinfo_revcolor=#84b0dc" /original_record="pOpen_v3.1" terminator 1950..1981 /label="tonB terminator" /note="/ApEinfo_fwdcolor=#b4abac /ApEinfo_revcolor=#b4abac" /original_record="pOpen_v3.1" CDS 2030..6208 /codon_start=1 /gene="LYS2" /product="LYS2 selectable marker" /label="LYS2" /translation="MTNEKVWIEKLDNPTLSVLPHDFLRPQQEPYTKQATYSLQLPQLD VPHDSFSNKYAVALSVWAALIYRVTGDDDIVLYIANNKILRFNIQPTWSFNELYSTINN ELNKLNSIEANFSFDELAEKIQSCQDLERTPQLFRLAFLENQDFKLDEFKHHLVDFALN LDTSNNAHVLNLIYNSLLYSNERVTIVADQFTQYLTAALSDPSNCITKISLITASSKDS LPDPTKNLGWCDFVGCIHDIFQDNAEAFPERTCVVETPTLNSDKSRSFTYRDINRTSNI VAHYLIKTGIKRGDVVMIYSSRGVDLMVCVMGVLKAGATFSVIDPAYPPARQTIYLGVA KPRGLIVIRAAGQLDQLVEDYINDELEIVSRINSIAIQENGTIEGGKLDNGEDVLAPYD HYKDTRTGVVVGPDSNPTLSFTSGSEGIPKGVLGRHFSLAYYFNWMSKRFNLTENDKFT MLSGIAHDPIQRDMFTPLFLGAQLYVPTQDDIGTPGRLAEWMSKYGCTVTHLTPAMGQL LTAQATTPFPKLHHAFFVGDILTKRDCLRLQTLAENCRIVNMYGTTETQRAVSYFEVKS KNDDPNFLKKLKDVMPAGKGMLNVQLLVVNRNDRTQICGIGEIGEIYVRAGGLAEGYRG LPELNKEKFVNNWFVEKDHWNYLDKDNGEPWRQFWLGPRDRLYRTGDLGRYLPNGDCEC CGRADDQVKIRGFRIELGEIDTHISQHPLVRENITLVRKNADNEPTLITFMVPRFDKPD DLSKFQSDVPKEVETDPIVKGLIGYHLLSKDIRTFLKKRLASYAMPSLIVVMDKLPLNP NGKVDKPKLQFPTPKQLNLVAENTVSETDDSQFTNVEREVRDLWLSILPTKPASVSPDD SFFDLGGHSILATKMIFTLKKKLQVDLPLGTIFKYPTIKAFAAEIDRIKSSGGSSQGEV VENVTANYAEDAKKLVETLPSSYPSREYFVEPNSAEGKTTINVFVTGVTGFLGSYILAD LLGRSPKNYSFKVFAHVRAKDEEAAFARLQKAGITYGTWNEKFASNIKVVLGDLSKSQF GLSDEKWMDLANTVDIIIHNGALVHWVYPYAKLRDPNVISTINVMSLAAVGKPKFFDFV SSTSTLDTEYYFNLSDKLVSEGKPGILESDDLMNSASGLTGGYGQSKWAAEYIIRRAGE RGLRGCIVRPGYVTGASANGSSNTDDFLLRFLKGSVQLGKIPDIENSVNMVPVDHVARV VVATSLNPPKENELAVAQVTGHPRILFKDYLYTLHDYGYDVEIESYSKWKKSLEASVID RNEENALYPLLHMVLDNLPESTKAPELDDRNAVASLKKDTAWTGVDWSNGIGVTPEEVG IYIAFLNKVGFLPPPTHNDKLPLPSIELTQAQISLVASGAGARGSSAAA" /original_record="part" protein_bind complement(join(6203..6205,6207..6213)) /label="SapI" /ApEinfo_fwdcolor="#00FF2C" /ApEinfo_revcolor="#00FF2C" /original_record="part" protein_bind 6214..6219 /label="HindIII" /ApEinfo_fwdcolor="#00FF2C" /ApEinfo_revcolor="#00FF2C" /original_record="part" misc_feature 2002..2021 /label="FreeGenes dialout primer" /ApEinfo_fwdcolor="#0048BA" /ApEinfo_revcolor="#0048BA" misc_feature complement(6227..6246) /label="FreeGenes dialout primer" /ApEinfo_fwdcolor="#0048BA" /ApEinfo_revcolor="#0048BA" misc_feature 6247..6306 /label="FreeGenes barcode" /ApEinfo_fwdcolor="#FF7E00" /ApEinfo_revcolor="#FF7E00" protein_bind join(2022..2027,2029..2032) /label="BsaI" /ApEinfo_fwdcolor="#00FF2C" /ApEinfo_revcolor="#00FF2C" protein_bind complement(join(6216..6219,6221..6226)) /label="BsaI" /ApEinfo_fwdcolor="#00FF2C" /ApEinfo_revcolor="#00FF2C" protein_bind 6268..6285 /label="I-SceI" /ApEinfo_fwdcolor="#4F0080" /ApEinfo_revcolor="#4F0080" ORIGIN 1 cgctgaggtg tcaatcgtcg gagccgctga gcaataacta gcataacccc ttggggcctc 61 taaacgggtc ttgaggggtt ttttgcatgg tcatagctgt ttcctgagag cttggcaggt 121 gatgacacac attaacaaat ttcgtgagga gtctccagaa gaatgccatt aatttccata 181 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc 241 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg 301 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc 361 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg 421 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc 481 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga 541 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg 601 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa 661 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg 721 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaaggccta ctattagcaa 781 caacgatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta 841 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa 901 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg 961 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg 1021 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc 1081 aatgataccg cgagaaccac gctcaccggc tccagattta tcagcaataa accagccagc 1141 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa 1201 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc 1261 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg 1321 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc 1381 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat 1441 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg 1501 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc 1561 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg 1621 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat 1681 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg 1741 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg 1801 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct 1861 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac 1921 ctgcaccagt cagtaaaacg acggccagta gtcaaaagcc tccgaccgga ggcttttgac 1981 ttggttcagg tggagtggga gaacgatgat gctcactctc gggtctctaa tgactaacga 2041 aaaggtctgg atagagaagt tggataatcc aactctttca gtgttaccac atgacttttt 2101 acgcccacaa caagaacctt atacgaaaca agctacatat tcgttacagc tacctcagct 2161 cgatgtgcct catgatagtt tttctaacaa atacgctgtc gctttgagtg tatgggctgc 2221 attgatatat agagtaaccg gtgacgatga tattgttctt tatattgcga ataacaaaat 2281 cttaagattc aatattcaac caacgtggtc atttaatgag ctgtattcta caattaacaa 2341 tgagttgaac aagctcaatt ctattgaggc caatttttcc tttgacgaac tagctgaaaa 2401 aattcaaagt tgccaagacc tggaaaggac ccctcagttg ttccgtttgg cctttttgga 2461 aaaccaagat ttcaaattag acgagttcaa gcatcattta gtggactttg ctttgaattt 2521 ggataccagt aataatgcgc atgttttgaa cttaatttat aacagcttac tgtattcgaa 2581 tgaaagagta accattgttg cggaccaatt tactcaatat ttgactgctg cgctaagcga 2641 tccatccaat tgcataacta aaatctctct gatcaccgca tcatccaagg atagtttacc 2701 tgatccaact aagaacttgg gctggtgcga tttcgtgggg tgtattcacg acattttcca 2761 ggacaatgct gaagccttcc cagagagaac ctgtgttgtg gagactccaa cactaaattc 2821 cgacaagtcc cgttctttca cttatcgtga catcaaccgc acttctaaca tagttgccca 2881 ttatttgatt aaaacaggta tcaaaagagg tgatgtagtg atgatctatt cttctagggg 2941 tgtggatttg atggtatgtg tgatgggtgt cttgaaagcc ggcgcaacct tttcagttat 3001 cgaccctgca tatcccccag ccagacaaac catttactta ggtgttgcta aaccacgtgg 3061 gttgattgtt attagagctg ctggacaatt ggatcaactg gtagaagatt acatcaatga 3121 tgaattggag attgtttcaa gaatcaattc cattgctatt caagaaaatg gtaccattga 3181 aggtggcaaa ttggacaatg gcgaggatgt tttggctcca tatgatcact acaaagacac 3241 cagaacaggt gttgtagttg gaccagattc caacccaacc ctatctttca catctggttc 3301 cgaaggtatt cctaagggtg ttcttggtag acatttttcc ttggcttatt atttcaattg 3361 gatgtccaaa aggttcaact taacagaaaa tgataaattc acaatgctga gcggtattgc 3421 acatgatcca attcaaagag atatgtttac accattattt ttaggtgccc aattgtatgt 3481 ccctactcaa gatgatattg gtacaccggg ccgtttagcg gaatggatga gtaagtatgg 3541 ttgcacagtt acccatttaa caccagccat gggtcaatta cttactgccc aagctactac 3601 accattccct aagttacatc atgcgttctt tgtgggtgac attttaacaa aacgtgattg 3661 tctgaggtta caaaccttgg cagaaaattg ccgtattgtt aatatgtacg gtaccactga 3721 aacacagcgt gcagtttctt atttcgaagt taaatcaaaa aatgacgatc caaacttttt 3781 gaaaaaattg aaagatgtca tgcctgctgg taaaggtatg ttgaacgttc agctgctagt 3841 tgttaacagg aacgatcgta ctcaaatatg tggtattggc gaaataggtg agatttatgt 3901 tcgtgcaggc ggtttggccg aaggttatag aggattacca gaattgaata aagaaaaatt 3961 tgtgaacaac tggtttgttg aaaaagatca ctggaattat ttggataagg ataatggtga 4021 accttggaga caattctggt taggtccaag agatagattg tacagaacgg gtgatttagg 4081 tcgttatcta ccaaacggtg actgtgaatg ttgcggtagg gctgatgatc aagttaaaat 4141 tcgtgggttc agaatcgaat taggagaaat agatacgcac atttcccaac atccattggt 4201 aagagaaaac attactttag ttcgcaaaaa tgccgacaat gagccaacat tgatcacatt 4261 tatggtccca agatttgaca agccagatga cttgtctaag ttccaaagtg atgttccaaa 4321 ggaggttgaa actgacccta tagttaaggg cttaatcggt taccatcttt tatccaagga 4381 catcaggact ttcttaaaga aaagattggc tagttatgct atgccttcct tgattgtggt 4441 tatggataaa ctaccattga atccaaatgg taaagttgat aagcctaaac ttcaattccc 4501 aactcccaag caattaaatt tggtagctga aaatacagtt tctgaaactg acgactctca 4561 gtttaccaat gttgagcgcg aggttagaga cttatggtta agtatattac ctaccaagcc 4621 agcatctgta tcaccagatg attcgttttt cgatttaggt ggtcattcta tcttggctac 4681 caaaatgatt tttaccttaa agaaaaagct gcaagttgat ttaccattgg gcacaatttt 4741 caagtatcca acgataaagg cctttgccgc ggaaattgac agaattaaat catcgggtgg 4801 atcatctcaa ggtgaggtcg tcgaaaatgt cactgcaaat tatgcggagg acgccaagaa 4861 attggttgaa acgctaccaa gttcgtaccc cagtagggaa tattttgttg aacctaatag 4921 tgccgaagga aaaacaacaa ttaatgtgtt tgttaccggt gtcacaggat ttctgggctc 4981 ctacatcctt gcagatttgt taggacgttc tccaaagaac tacagtttca aagtgtttgc 5041 ccacgtcagg gccaaggatg aagaagctgc atttgcaaga ttacaaaagg caggtatcac 5101 ctatggtact tggaacgaaa aatttgcctc aaatattaaa gttgtattag gcgatttatc 5161 taaaagccaa tttggtcttt cagatgagaa gtggatggat ttggcaaaca cagttgatat 5221 aattatccat aatggtgcgt tagttcactg ggtttatcca tatgccaaat tgagggaccc 5281 aaatgttatt tcaactatca atgttatgag cttagccgcc gtcggcaagc caaagttctt 5341 tgactttgtt tcctccactt ctactcttga cactgaatac tactttaatt tgtcagataa 5401 acttgttagc gaagggaagc caggcatttt agaatcagac gatttaatga actctgcaag 5461 cgggctcact ggtggatatg gtcagtccaa atgggctgct gagtacatca ttagacgtgc 5521 cggtgaaagg ggcctacgtg ggtgtattgt cagaccaggt tacgtaacag gtgcctctgc 5581 caatggttct tcaaacacag atgatttctt attgagattt ttgaaaggtt cagtccaatt 5641 aggtaagatt ccagatatcg aaaattccgt gaatatggtt ccagtagatc atgttgctcg 5701 tgttgttgtt gctacgtctt tgaatcctcc caaagaaaat gaattggccg ttgctcaagt 5761 aacgggtcac ccaagaatat tattcaaaga ctacttgtat actttacacg attatggtta 5821 cgatgtcgaa atcgaaagct attctaaatg gaagaaatca ttggaggcgt ctgttattga 5881 caggaatgaa gaaaatgcgt tgtatccttt gctacacatg gtcttagaca acttacctga 5941 aagtaccaaa gctccggaac tagacgatag gaacgccgtg gcatctttaa agaaagacac 6001 cgcatggaca ggtgttgatt ggtctaatgg aataggtgtt actccagaag aggttggtat 6061 atatattgca tttttaaaca aggttggatt tttacctcca ccaactcata atgacaaact 6121 tccactgcca agtatagaac taactcaagc gcaaataagt ctagttgctt caggtgctgg 6181 tgctcgtgga agctccgcag cagcttgaag agcaagcttt gagaccagat agtcacgcac 6241 aagaccatct ctaagtgata aaaactttag ggataacagg gtaatgtgaa tggccaatgt 6301 gaaaga //