LOCUS       Exported     3667 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3667
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      38..85
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     1933..1949
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      1950..1981
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
                     /label="tonB terminator"
     CDS             2010..3653
                     /label="MMSYN1_0420"
                     /note="yloV:DAK2 domain fusion protein YloV"
                     /translation="MKKLELLKNMITSGVNNLYNHYPQIDKLNVFPVPDGDTGTNMNLTATNGYNEVIDVEYESIGKFLSAFSRGLIMGARGNSGVIFSQIIKGLSLGMNNAKELSVSEWKSGFSKASEIAYKAVMKPVEGTILTVIRETSEKVSQLADDIDIKDFWKQVVKNANQSLENTPNLLPLLKEVGVVDSGGYGLVKFLEGIEYYVLNDQIVNKLDKLEVNNGGNVDMQIEEEFGYCTEAIVMLNDDWINKLQNSVIRDQLQIFGNTSIVVVVDNDILKVHTHSLSPGQVLQFLQQYGDFQTLKIENMNLQANKQVKNKDQKWKENSDIKTERKLINETAIISVVSSEKQKRYFEDELGIAFAINAGAKMNPSTEDFLQAIETVDAKTVFLLPNSSNVYLTAKQAEKIENKSKIYVIQTKTIQQGMVAALSFDPSLTASKNYSYLSKSFRNVVSFNITKAEKNTTYNGIEIQKDNLLAIVDNNIIGAEQTLEAIFDKQLSKYIKSKTEIITIFVGGETNEQDLVQLRKFLDEGYDVEYEIFDGGQETYNLLIAIE"
     primer_bind     complement(90..106)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(173..761)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(952..1812)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(1813..1917)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC
       61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT
      121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA
      181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC
      241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG
      301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC
      361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG
      421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC
      481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA
      541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG
      601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA
      661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG
      721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA
      781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA
      841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA
      901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG
      961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG
     1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC
     1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC
     1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA
     1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC
     1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG
     1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC
     1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT
     1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG
     1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC
     1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG
     1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT
     1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG
     1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG
     1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT
     1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC
     1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC
     1981 TTGGTTCAGG TGGAGTGGGA GGGTCTCAAA TGAAAAAGCT TGAATTATTA AAGAATATGA
     2041 TTACCTCAGG AGTTAACAAC CTTTATAACC ATTACCCGCA AATCGACAAG CTGAATGTGT
     2101 TTCCAGTGCC CGACGGAGAC ACGGGGACGA ACATGAATTT AACCGCAACT AACGGGTACA
     2161 ACGAGGTAAT TGATGTAGAA TACGAAAGTA TCGGCAAATT CCTGAGTGCT TTCTCTCGTG
     2221 GACTGATTAT GGGCGCACGC GGGAATAGCG GGGTGATTTT CAGTCAGATC ATCAAAGGGT
     2281 TATCCTTAGG TATGAACAAC GCAAAAGAGC TTAGCGTCAG CGAATGGAAG TCGGGTTTCT
     2341 CAAAAGCGTC CGAGATCGCG TACAAAGCAG TAATGAAACC CGTTGAGGGG ACAATTCTGA
     2401 CTGTCATCCG CGAAACAAGT GAAAAAGTTA GTCAATTGGC TGACGACATC GATATCAAGG
     2461 ATTTTTGGAA ACAAGTAGTA AAGAATGCTA ACCAAAGCTT AGAGAATACA CCGAATCTTC
     2521 TGCCCCTTCT GAAAGAAGTT GGTGTTGTTG ACAGTGGCGG TTACGGATTA GTGAAATTTC
     2581 TGGAGGGTAT TGAGTACTAT GTGCTTAATG ACCAAATCGT AAATAAATTA GATAAATTGG
     2641 AAGTTAACAA CGGCGGGAAT GTCGATATGC AGATCGAGGA AGAGTTTGGT TACTGCACGG
     2701 AGGCGATCGT TATGCTGAAT GATGACTGGA TTAACAAGTT GCAAAATTCA GTGATCCGCG
     2761 ATCAACTTCA AATCTTTGGG AACACCTCGA TCGTCGTGGT AGTCGATAAT GACATCCTGA
     2821 AGGTTCATAC GCATTCGCTT AGCCCTGGGC AAGTTCTTCA ATTTCTGCAG CAGTACGGTG
     2881 ATTTTCAGAC GCTGAAGATC GAGAACATGA ACTTACAGGC TAACAAGCAA GTTAAAAACA
     2941 AAGACCAGAA ATGGAAAGAG AACAGTGACA TCAAGACAGA ACGCAAGCTG ATCAACGAAA
     3001 CCGCGATCAT TTCTGTTGTA TCCTCTGAGA AACAAAAGCG TTATTTTGAG GACGAACTTG
     3061 GGATCGCGTT CGCTATTAAC GCTGGAGCAA AGATGAACCC GAGCACCGAG GACTTCCTTC
     3121 AGGCTATTGA AACCGTGGAT GCCAAAACAG TCTTTTTGTT ACCTAACTCC TCAAATGTCT
     3181 ACCTGACGGC GAAGCAGGCA GAGAAGATCG AAAACAAAAG CAAGATTTAT GTGATTCAAA
     3241 CCAAAACGAT CCAGCAGGGA ATGGTCGCAG CGTTAAGCTT TGACCCTAGT TTAACCGCAA
     3301 GTAAGAATTA CAGTTACCTG TCAAAGTCAT TTCGTAATGT AGTTTCTTTC AACATCACGA
     3361 AGGCTGAGAA GAATACAACG TATAACGGCA TTGAGATTCA AAAAGACAAC CTTCTGGCAA
     3421 TTGTGGACAA CAACATTATT GGTGCAGAAC AAACCTTGGA GGCTATCTTC GACAAGCAAT
     3481 TGAGCAAATA CATCAAATCT AAAACTGAGA TTATCACGAT TTTTGTCGGC GGAGAAACGA
     3541 ACGAACAGGA CTTGGTTCAG CTGCGTAAAT TCTTAGATGA AGGGTATGAC GTGGAATATG
     3601 AGATTTTCGA CGGAGGGCAA GAGACTTATA ATCTTTTGAT CGCCATTGAG TGAAGAGCTT
     3661 AGAGACC
//