LOCUS       Exported     3188 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3188
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1201..1248
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     3076..3092
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      3093..3124
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             1..0
                     /label="MMSYN1_0425"
                     /note="PstS: phosphate binding protein"
                     /translation="MRKIKESDNKTKKQFKHFFTNKKSLLFLNLCVLFIVSIWIWTFVSIKSNLINIGGSASADLVLQKLIKEYQKQTNKKFNYSSTGSGAGARNVINQTYSIGFISKSQNDLSIPKEIRNNLYDDLTEFEKINDELSKKQVFNHLKTFKDKYHYIDFVKDSIVFVYNIKNTGLTNQQIDQIRFNVLNNKISSDSQKALHKIYTNNKQSDLISWKEFYKLLTNKDENNISSLIKVRPYSTNSGSGTRSSFEQISGLKDNNKKIGQAVNEYNSNGAIFTQLDASDGSFGFVSMQYAQDLKKYPNLRSVIIKQNNQEWNLNKRNDNLLTYPLSRPFVALYKFTDNQKLNDDILDFVYWFSYSNDQKVKKIYDHLGLIRKVSN"
     primer_bind     complement(1253..1269)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1336..1924)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(2095..2955)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(2956..3060)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
     CDS             complement(1..0)
                     /label="MMSYN1_0425"
                     /note="PstS: phosphate binding protein"
                     /translation="MRKIKESDNKTKKQFKHFFTNKKSLLFLNLCVLFIVSIWIWTFVSIKSNLINIGGSASADLVLQKLIKEYQKQTNKKFNYSSTGSGAGARNVINQTYSIGFISKSQNDLSIPKEIRNNLYDDLTEFEKINDELSKKQVFNHLKTFKDKYHYIDFVKDSIVFVYNIKNTGLTNQQIDQIRFNVLNNKISSDSQKALHKIYTNNKQSDLISWKEFYKLLTNKDENNISSLIKVRPYSTNSGSGTRSSFEQISGLKDNNKKIGQAVNEYNSNGAIFTQLDASDGSFGFVSMQYAQDLKKYPNLRSVIIKQNNQEWNLNKRNDNLLTYPLSRPFVALYKFTDNQKLNDDILDFVYWFSYSNDQKVKKIYDHLGLIRKVSN"
ORIGIN
        1 ATGCGCAAGA TTAAGGAGTC TGATAATAAA ACGAAAAAGC AATTCAAACA TTTCTTCACA
       61 AATAAGAAAT CTTTACTGTT TTTAAACCTG TGTGTTTTAT TTATCGTATC GATTTGGATC
      121 TGGACCTTTG TGAGCATTAA ATCCAATCTG ATTAATATTG GCGGATCTGC ATCGGCAGAT
      181 CTTGTTCTGC AAAAGCTTAT TAAGGAGTAC CAGAAACAGA CCAACAAAAA GTTTAATTAT
      241 TCCTCGACAG GCTCTGGCGC TGGCGCACGC AACGTAATCA ACCAGACCTA TTCAATCGGC
      301 TTTATCAGCA AGTCCCAGAA CGATCTTAGC ATTCCTAAGG AGATTCGCAA TAACCTTTAC
      361 GATGACCTGA CTGAGTTTGA GAAAATTAAC GATGAATTGA GTAAAAAACA GGTGTTTAAT
      421 CACTTGAAAA CTTTTAAGGA TAAATACCAC TATATCGATT TTGTGAAAGA CTCAATTGTC
      481 TTTGTATATA ATATCAAAAA TACCGGACTG ACCAATCAAC AGATCGATCA AATCCGTTTC
      541 AATGTCTTGA ATAATAAAAT CTCTTCAGAC TCTCAAAAGG CCCTGCATAA AATCTACACA
      601 AATAACAAGC AATCGGACCT GATTTCATGG AAGGAATTCT ATAAGCTTCT GACAAATAAA
      661 GATGAAAATA ATATTTCATC CTTAATTAAA GTACGTCCAT ACTCCACTAA CAGCGGCAGC
      721 GGTACGCGCT CCTCGTTCGA ACAAATCTCA GGCTTGAAGG ACAATAATAA GAAGATTGGG
      781 CAAGCAGTGA ACGAGTACAA CAGCAACGGC GCTATTTTCA CACAACTGGA CGCATCAGAT
      841 GGCTCATTCG GATTTGTTTC CATGCAGTAT GCGCAGGACT TAAAAAAGTA TCCGAACTTG
      901 CGTAGCGTGA TTATCAAACA GAACAATCAA GAGTGGAACC TTAATAAGCG TAATGACAAC
      961 TTGCTGACCT ATCCGTTGTC ACGCCCATTT GTAGCTTTGT ACAAGTTTAC GGATAACCAG
     1021 AAACTTAACG ATGATATTCT TGACTTCGTC TATTGGTTTT CCTACTCAAA CGACCAGAAG
     1081 GTCAAGAAAA TCTACGATCA TTTAGGGTTG ATTCGCAAAG TCAGCAACTG AAGAGCTTAG
     1141 AGACCGGTAC TAGTTCCTGC AGGGCTCGAG GTGTCAATCG TCGGAGCCGC TGAGCAATAA
     1201 CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCA TGGTCATAGC
     1261 TGTTTCCTGA GAGCTTGGCA GGTGATGACA CACATTAACA AATTTCGTGA GGAGTCTCCA
     1321 GAAGAATGCC ATTAATTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG
     1381 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG
     1441 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT
     1501 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT
     1561 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG
     1621 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT
     1681 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT
     1741 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT
     1801 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC
     1861 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC
     1921 TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG
     1981 TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA
     2041 AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA ACTTGGTCTG ACAGTTACCA
     2101 ATGCTTAATC AGTGAGGCAC CTATCTCAGC GATCTGTCTA TTTCGTTCAT CCATAGTTGC
     2161 CTGACTCCCC GTCGTGTAGA TAACTACGAT ACGGGAGGGC TTACCATCTG GCCCCAGTGC
     2221 TGCAATGATA CCGCGAGAAC CACGCTCACC GGCTCCAGAT TTATCAGCAA TAAACCAGCC
     2281 AGCCGGAAGG GCCGAGCGCA GAAGTGGTCC TGCAACTTTA TCCGCCTCCA TCCAGTCTAT
     2341 TAATTGTTGC CGGGAAGCTA GAGTAAGTAG TTCGCCAGTT AATAGTTTGC GCAACGTTGT
     2401 TGCCATTGCT ACAGGCATCG TGGTGTCACG CTCGTCGTTT GGTATGGCTT CATTCAGCTC
     2461 CGGTTCCCAA CGATCAAGGC GAGTTACATG ATCCCCCATG TTGTGCAAAA AAGCGGTTAG
     2521 CTCCTTCGGT CCTCCGATCG TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT CACTCATGGT
     2581 TATGGCAGCA CTGCATAATT CTCTTACTGT CATGCCATCC GTAAGATGCT TTTCTGTGAC
     2641 TGGTGAGTAC TCAACCAAGT CATTCTGAGA ATAGTGTATG CGGCGACCGA GTTGCTCTTG
     2701 CCCGGCGTCA ATACGGGATA ATACCGCGCC ACATAGCAGA ACTTTAAAAG TGCTCATCAT
     2761 TGGAAAACGT TCTTCGGGGC GAAAACTCTC AAGGATCTTA CCGCTGTTGA GATCCAGTTC
     2821 GATGTAACCC ACTCGTGCAC CCAACTGATC TTCAGCATCT TTTACTTTCA CCAGCGTTTC
     2881 TGGGTGAGCA AAAACAGGAA GGCAAAATGC CGCAAAAAAG GGAATAAGGG CGACACGGAA
     2941 ATGTTGAATA CTCATACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG
     3001 TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG
     3061 CACCTGCACC AGTCAGTAAA ACGACGGCCA GTAGTCAAAA GCCTCCGACC GGAGGCTTTT
     3121 GACTTGGTCA GGTGGAGTGA ATTCGCGGCC GCTTCTAGAG GCTCTTCGTA ATTACGAAGA
     3181 GGTCTCTA
//