LOCUS Exported 3188 bp DNA circular SYN 24-JUL-2020 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2001) AUTHORS Keoni Gandall TITLE Direct Submission JOURNAL Exported from Poly. FEATURES Location/Qualifiers terminator 1..3188 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" terminator 1201..1248 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" primer_bind 3076..3092 /label="M13 for" /note="common sequencing primer, one of multiple similar variants" terminator 3093..3124 /label="tonB terminator" /note="bidirectional E. coli tonB-P14 transcription terminator" CDS 1..0 /label="MMSYN1_0425" /note="PstS: phosphate binding protein" /translation="MRKIKESDNKTKKQFKHFFTNKKSLLFLNLCVLFIVSIWIWTFVSIKSNLINIGGSASADLVLQKLIKEYQKQTNKKFNYSSTGSGAGARNVINQTYSIGFISKSQNDLSIPKEIRNNLYDDLTEFEKINDELSKKQVFNHLKTFKDKYHYIDFVKDSIVFVYNIKNTGLTNQQIDQIRFNVLNNKISSDSQKALHKIYTNNKQSDLISWKEFYKLLTNKDENNISSLIKVRPYSTNSGSGTRSSFEQISGLKDNNKKIGQAVNEYNSNGAIFTQLDASDGSFGFVSMQYAQDLKKYPNLRSVIIKQNNQEWNLNKRNDNLLTYPLSRPFVALYKFTDNQKLNDDILDFVYWFSYSNDQKVKKIYDHLGLIRKVSN" primer_bind complement(1253..1269) /label="M13 rev" /note="common sequencing primer, one of multiple similar variants" rep_origin complement(1336..1924) /label="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(2095..2955) /label="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*" promoter complement(2956..3060) /label="bla promoter" /note="ampicillin resistance gene promoter" CDS complement(1..0) /label="MMSYN1_0425" /note="PstS: phosphate binding protein" /translation="MRKIKESDNKTKKQFKHFFTNKKSLLFLNLCVLFIVSIWIWTFVSIKSNLINIGGSASADLVLQKLIKEYQKQTNKKFNYSSTGSGAGARNVINQTYSIGFISKSQNDLSIPKEIRNNLYDDLTEFEKINDELSKKQVFNHLKTFKDKYHYIDFVKDSIVFVYNIKNTGLTNQQIDQIRFNVLNNKISSDSQKALHKIYTNNKQSDLISWKEFYKLLTNKDENNISSLIKVRPYSTNSGSGTRSSFEQISGLKDNNKKIGQAVNEYNSNGAIFTQLDASDGSFGFVSMQYAQDLKKYPNLRSVIIKQNNQEWNLNKRNDNLLTYPLSRPFVALYKFTDNQKLNDDILDFVYWFSYSNDQKVKKIYDHLGLIRKVSN" ORIGIN 1 ATGCGCAAGA TTAAGGAGTC TGATAATAAA ACGAAAAAGC AATTCAAACA TTTCTTCACA 61 AATAAGAAAT CTTTACTGTT TTTAAACCTG TGTGTTTTAT TTATCGTATC GATTTGGATC 121 TGGACCTTTG TGAGCATTAA ATCCAATCTG ATTAATATTG GCGGATCTGC ATCGGCAGAT 181 CTTGTTCTGC AAAAGCTTAT TAAGGAGTAC CAGAAACAGA CCAACAAAAA GTTTAATTAT 241 TCCTCGACAG GCTCTGGCGC TGGCGCACGC AACGTAATCA ACCAGACCTA TTCAATCGGC 301 TTTATCAGCA AGTCCCAGAA CGATCTTAGC ATTCCTAAGG AGATTCGCAA TAACCTTTAC 361 GATGACCTGA CTGAGTTTGA GAAAATTAAC GATGAATTGA GTAAAAAACA GGTGTTTAAT 421 CACTTGAAAA CTTTTAAGGA TAAATACCAC TATATCGATT TTGTGAAAGA CTCAATTGTC 481 TTTGTATATA ATATCAAAAA TACCGGACTG ACCAATCAAC AGATCGATCA AATCCGTTTC 541 AATGTCTTGA ATAATAAAAT CTCTTCAGAC TCTCAAAAGG CCCTGCATAA AATCTACACA 601 AATAACAAGC AATCGGACCT GATTTCATGG AAGGAATTCT ATAAGCTTCT GACAAATAAA 661 GATGAAAATA ATATTTCATC CTTAATTAAA GTACGTCCAT ACTCCACTAA CAGCGGCAGC 721 GGTACGCGCT CCTCGTTCGA ACAAATCTCA GGCTTGAAGG ACAATAATAA GAAGATTGGG 781 CAAGCAGTGA ACGAGTACAA CAGCAACGGC GCTATTTTCA CACAACTGGA CGCATCAGAT 841 GGCTCATTCG GATTTGTTTC CATGCAGTAT GCGCAGGACT TAAAAAAGTA TCCGAACTTG 901 CGTAGCGTGA TTATCAAACA GAACAATCAA GAGTGGAACC TTAATAAGCG TAATGACAAC 961 TTGCTGACCT ATCCGTTGTC ACGCCCATTT GTAGCTTTGT ACAAGTTTAC GGATAACCAG 1021 AAACTTAACG ATGATATTCT TGACTTCGTC TATTGGTTTT CCTACTCAAA CGACCAGAAG 1081 GTCAAGAAAA TCTACGATCA TTTAGGGTTG ATTCGCAAAG TCAGCAACTG AAGAGCTTAG 1141 AGACCGGTAC TAGTTCCTGC AGGGCTCGAG GTGTCAATCG TCGGAGCCGC TGAGCAATAA 1201 CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCA TGGTCATAGC 1261 TGTTTCCTGA GAGCTTGGCA GGTGATGACA CACATTAACA AATTTCGTGA GGAGTCTCCA 1321 GAAGAATGCC ATTAATTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG 1381 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG 1441 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT 1501 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT 1561 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG 1621 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT 1681 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT 1741 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT 1801 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC 1861 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC 1921 TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG 1981 TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA 2041 AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA ACTTGGTCTG ACAGTTACCA 2101 ATGCTTAATC AGTGAGGCAC CTATCTCAGC GATCTGTCTA TTTCGTTCAT CCATAGTTGC 2161 CTGACTCCCC GTCGTGTAGA TAACTACGAT ACGGGAGGGC TTACCATCTG GCCCCAGTGC 2221 TGCAATGATA CCGCGAGAAC CACGCTCACC GGCTCCAGAT TTATCAGCAA TAAACCAGCC 2281 AGCCGGAAGG GCCGAGCGCA GAAGTGGTCC TGCAACTTTA TCCGCCTCCA TCCAGTCTAT 2341 TAATTGTTGC CGGGAAGCTA GAGTAAGTAG TTCGCCAGTT AATAGTTTGC GCAACGTTGT 2401 TGCCATTGCT ACAGGCATCG TGGTGTCACG CTCGTCGTTT GGTATGGCTT CATTCAGCTC 2461 CGGTTCCCAA CGATCAAGGC GAGTTACATG ATCCCCCATG TTGTGCAAAA AAGCGGTTAG 2521 CTCCTTCGGT CCTCCGATCG TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT CACTCATGGT 2581 TATGGCAGCA CTGCATAATT CTCTTACTGT CATGCCATCC GTAAGATGCT TTTCTGTGAC 2641 TGGTGAGTAC TCAACCAAGT CATTCTGAGA ATAGTGTATG CGGCGACCGA GTTGCTCTTG 2701 CCCGGCGTCA ATACGGGATA ATACCGCGCC ACATAGCAGA ACTTTAAAAG TGCTCATCAT 2761 TGGAAAACGT TCTTCGGGGC GAAAACTCTC AAGGATCTTA CCGCTGTTGA GATCCAGTTC 2821 GATGTAACCC ACTCGTGCAC CCAACTGATC TTCAGCATCT TTTACTTTCA CCAGCGTTTC 2881 TGGGTGAGCA AAAACAGGAA GGCAAAATGC CGCAAAAAAG GGAATAAGGG CGACACGGAA 2941 ATGTTGAATA CTCATACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG 3001 TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG 3061 CACCTGCACC AGTCAGTAAA ACGACGGCCA GTAGTCAAAA GCCTCCGACC GGAGGCTTTT 3121 GACTTGGTCA GGTGGAGTGA ATTCGCGGCC GCTTCTAGAG GCTCTTCGTA ATTACGAAGA 3181 GGTCTCTA //