LOCUS       Exported     4114 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..4114
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      38..85
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     1933..1949
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      1950..1981
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             2010..4100
                     /label="MMSYN1_0426"
                     /note=""
                     /translation="MLFKKITNNVQFRMIKQKKETSFVCLKSIIITLTIFVLLALVILLGFVMIKTNVLFNKQSFFEFVFGKNWSPDSKQFGILTITLMTLILIFISMLIAVPLTIFTSFFISEYLTLKSQKVTITIIKLLAGIPSVVFGLFAREQIGALFKLMGASSNDNLMVASLTMAFMAIPIMISLSYDAIKSVPFIYRDASLALGISKEKTTFNIIRKSATPKIISAVILGMARVIGETMAIMMIAGNSTAWFDTNNGVSGFLFSSIRTLSSTIGLEMLENSSSLHESALYAIGMFLFILVFIINLLILFVSNKNSISKKIHLVLFKNKKTHKIKHVKLYQKQTLDQIITNRTENKLFKKIYSTIMLVLMWLSISFVIMFTFWIVFTTIFNGLSSLKYSEAFLTIEGEDGIFAAILTTLLLILCTLLFAIPLALACAIYLSEFANKNSYFAKFFRFLLNLAASTPSIIFGIFGLSVFIIYLKLPFSIFSASITMTIVVLPMLIKNFEDALTSVPLSYREAAIALGLSKTKTLFKIVLPNALQAIITGTILAMARIIGESAPIYLTLGTAIKYPDRGFLSSGSTLTTGIYKIASESAPGQGNDIAWLMSLITIIFVLTLNLSSSKLSLLLVKTNKKVKFEFKQIYKNFINKQFYKTQFNLFKNSLKQFIKSLKKYLNITNLFKEIKKTIKYKKEYKKLKKRDNNYE"
     primer_bind     complement(90..106)
                     /note="common sequencing primer, one of multiple similar variants"
                     /label="M13 rev"
     rep_origin      complement(173..761)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(952..1812)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(1813..1917)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC
       61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT
      121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA
      181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC
      241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG
      301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC
      361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG
      421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC
      481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA
      541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG
      601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA
      661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG
      721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA
      781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA
      841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA
      901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG
      961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG
     1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC
     1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC
     1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA
     1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC
     1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG
     1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC
     1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT
     1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG
     1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC
     1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG
     1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT
     1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG
     1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG
     1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT
     1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC
     1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC
     1981 TTGGTTCAGG TGGAGTGGGA GGGTCTCAAA TGCTTTTCAA GAAGATTACG AATAACGTCC
     2041 AATTCCGTAT GATCAAGCAG AAGAAGGAAA CCTCTTTCGT GTGCCTTAAG TCTATTATTA
     2101 TCACTTTGAC GATTTTCGTT TTGCTTGCTT TGGTAATCTT GCTGGGGTTT GTTATGATTA
     2161 AGACGAATGT CTTGTTTAAC AAGCAAAGCT TTTTCGAATT CGTATTTGGC AAAAACTGGT
     2221 CACCTGACAG CAAACAGTTC GGCATCCTGA CTATTACCCT TATGACCCTT ATCTTGATCT
     2281 TCATCTCTAT GTTAATTGCC GTGCCACTTA CAATCTTTAC ATCTTTTTTC ATCTCAGAAT
     2341 ACCTTACTTT GAAAAGCCAA AAGGTGACAA TTACCATCAT TAAATTGTTG GCGGGGATTC
     2401 CCAGTGTCGT GTTTGGGCTG TTTGCGCGCG AACAGATCGG CGCCCTTTTT AAATTAATGG
     2461 GTGCTAGCTC TAACGACAAC TTGATGGTGG CAAGTCTTAC CATGGCGTTT ATGGCGATTC
     2521 CCATCATGAT CTCACTGTCT TATGATGCGA TTAAAAGTGT CCCCTTCATT TACCGTGACG
     2581 CTAGTCTGGC GCTTGGGATC TCAAAGGAGA AAACGACCTT TAACATCATT CGCAAATCGG
     2641 CTACTCCAAA GATCATTAGC GCGGTTATCT TGGGAATGGC GCGTGTTATT GGTGAGACTA
     2701 TGGCCATCAT GATGATTGCG GGTAACTCCA CGGCGTGGTT TGATACCAAC AACGGTGTCT
     2761 CTGGTTTTCT TTTCTCCTCA ATTCGCACCT TGAGCAGTAC AATCGGTTTG GAGATGTTGG
     2821 AAAATTCTTC ATCGTTGCAT GAGAGCGCAC TGTACGCGAT TGGAATGTTT CTGTTCATCT
     2881 TGGTGTTTAT TATTAACTTG CTTATCTTGT TTGTTAGTAA TAAGAACAGT ATCAGTAAAA
     2941 AGATCCACCT TGTATTGTTT AAAAACAAGA AAACTCACAA AATTAAGCAT GTCAAACTTT
     3001 ACCAGAAACA GACCCTTGAT CAGATCATTA CTAATCGTAC TGAGAATAAA TTATTTAAGA
     3061 AAATTTACTC CACGATCATG TTGGTCCTTA TGTGGTTGTC TATTAGTTTT GTTATCATGT
     3121 TTACATTCTG GATTGTTTTC ACGACCATTT TCAACGGCCT GTCATCTTTG AAATATTCAG
     3181 AGGCTTTCTT GACTATCGAA GGGGAAGATG GAATCTTCGC CGCAATCTTA ACCACACTGC
     3241 TTCTTATTTT GTGCACTCTT TTATTTGCAA TCCCGTTAGC GTTAGCATGC GCGATCTATC
     3301 TGTCCGAATT CGCAAATAAA AATTCTTACT TTGCCAAATT CTTTCGTTTT CTGTTGAATT
     3361 TGGCTGCATC TACGCCTTCG ATTATTTTCG GTATTTTTGG ACTGAGCGTA TTCATCATTT
     3421 ATCTGAAGTT ACCGTTCTCT ATCTTCAGTG CGTCCATTAC CATGACGATT GTTGTGCTGC
     3481 CAATGCTTAT CAAAAACTTT GAGGACGCCC TGACTAGCGT TCCATTGAGC TATCGTGAGG
     3541 CAGCAATCGC CCTTGGTCTT AGCAAGACTA AGACCTTGTT CAAGATTGTT CTTCCGAATG
     3601 CCCTTCAAGC CATTATCACA GGCACTATTC TGGCTATGGC GCGCATCATT GGTGAGTCCG
     3661 CCCCGATTTA TTTGACACTT GGTACGGCGA TTAAATATCC AGACCGTGGG TTTCTTAGCA
     3721 GTGGAAGTAC ATTGACAACG GGTATTTACA AAATCGCGTC GGAGTCGGCT CCAGGACAAG
     3781 GGAACGACAT TGCATGGTTG ATGAGTTTGA TCACCATTAT CTTTGTCTTA ACCTTGAATT
     3841 TGTCATCCAG TAAATTAAGC CTTCTGTTAG TAAAAACTAA CAAGAAAGTA AAATTTGAGT
     3901 TTAAACAAAT CTATAAGAAC TTTATTAATA AGCAATTTTA CAAGACGCAA TTTAACTTAT
     3961 TCAAGAATTC ACTTAAGCAA TTTATTAAAT CCCTGAAAAA GTACTTAAAT ATTACGAACC
     4021 TTTTCAAGGA AATTAAGAAA ACTATTAAGT ACAAGAAAGA GTACAAAAAA TTAAAGAAAC
     4081 GCGATAATAA TTACGAGTGA AGAGCTTAGA GACC
//