LOCUS Exported 4114 bp DNA circular SYN 24-JUL-2020 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2001) AUTHORS Keoni Gandall TITLE Direct Submission JOURNAL Exported from Poly. FEATURES Location/Qualifiers terminator 1..4114 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" terminator 38..85 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" primer_bind 1933..1949 /label="M13 for" /note="common sequencing primer, one of multiple similar variants" terminator 1950..1981 /label="tonB terminator" /note="bidirectional E. coli tonB-P14 transcription terminator" CDS 2010..4100 /label="MMSYN1_0426" /note="" /translation="MLFKKITNNVQFRMIKQKKETSFVCLKSIIITLTIFVLLALVILLGFVMIKTNVLFNKQSFFEFVFGKNWSPDSKQFGILTITLMTLILIFISMLIAVPLTIFTSFFISEYLTLKSQKVTITIIKLLAGIPSVVFGLFAREQIGALFKLMGASSNDNLMVASLTMAFMAIPIMISLSYDAIKSVPFIYRDASLALGISKEKTTFNIIRKSATPKIISAVILGMARVIGETMAIMMIAGNSTAWFDTNNGVSGFLFSSIRTLSSTIGLEMLENSSSLHESALYAIGMFLFILVFIINLLILFVSNKNSISKKIHLVLFKNKKTHKIKHVKLYQKQTLDQIITNRTENKLFKKIYSTIMLVLMWLSISFVIMFTFWIVFTTIFNGLSSLKYSEAFLTIEGEDGIFAAILTTLLLILCTLLFAIPLALACAIYLSEFANKNSYFAKFFRFLLNLAASTPSIIFGIFGLSVFIIYLKLPFSIFSASITMTIVVLPMLIKNFEDALTSVPLSYREAAIALGLSKTKTLFKIVLPNALQAIITGTILAMARIIGESAPIYLTLGTAIKYPDRGFLSSGSTLTTGIYKIASESAPGQGNDIAWLMSLITIIFVLTLNLSSSKLSLLLVKTNKKVKFEFKQIYKNFINKQFYKTQFNLFKNSLKQFIKSLKKYLNITNLFKEIKKTIKYKKEYKKLKKRDNNYE" primer_bind complement(90..106) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" rep_origin complement(173..761) /label="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(952..1812) /label="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*" promoter complement(1813..1917) /label="bla promoter" /note="ampicillin resistance gene promoter" ORIGIN 1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC 61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT 121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA 181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC 241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG 301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC 361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG 421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC 481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA 541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG 601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA 661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG 721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA 781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA 841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA 901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG 961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG 1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC 1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC 1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA 1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC 1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG 1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC 1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT 1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG 1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC 1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG 1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT 1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG 1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG 1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT 1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC 1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC 1981 TTGGTTCAGG TGGAGTGGGA GGGTCTCAAA TGCTTTTCAA GAAGATTACG AATAACGTCC 2041 AATTCCGTAT GATCAAGCAG AAGAAGGAAA CCTCTTTCGT GTGCCTTAAG TCTATTATTA 2101 TCACTTTGAC GATTTTCGTT TTGCTTGCTT TGGTAATCTT GCTGGGGTTT GTTATGATTA 2161 AGACGAATGT CTTGTTTAAC AAGCAAAGCT TTTTCGAATT CGTATTTGGC AAAAACTGGT 2221 CACCTGACAG CAAACAGTTC GGCATCCTGA CTATTACCCT TATGACCCTT ATCTTGATCT 2281 TCATCTCTAT GTTAATTGCC GTGCCACTTA CAATCTTTAC ATCTTTTTTC ATCTCAGAAT 2341 ACCTTACTTT GAAAAGCCAA AAGGTGACAA TTACCATCAT TAAATTGTTG GCGGGGATTC 2401 CCAGTGTCGT GTTTGGGCTG TTTGCGCGCG AACAGATCGG CGCCCTTTTT AAATTAATGG 2461 GTGCTAGCTC TAACGACAAC TTGATGGTGG CAAGTCTTAC CATGGCGTTT ATGGCGATTC 2521 CCATCATGAT CTCACTGTCT TATGATGCGA TTAAAAGTGT CCCCTTCATT TACCGTGACG 2581 CTAGTCTGGC GCTTGGGATC TCAAAGGAGA AAACGACCTT TAACATCATT CGCAAATCGG 2641 CTACTCCAAA GATCATTAGC GCGGTTATCT TGGGAATGGC GCGTGTTATT GGTGAGACTA 2701 TGGCCATCAT GATGATTGCG GGTAACTCCA CGGCGTGGTT TGATACCAAC AACGGTGTCT 2761 CTGGTTTTCT TTTCTCCTCA ATTCGCACCT TGAGCAGTAC AATCGGTTTG GAGATGTTGG 2821 AAAATTCTTC ATCGTTGCAT GAGAGCGCAC TGTACGCGAT TGGAATGTTT CTGTTCATCT 2881 TGGTGTTTAT TATTAACTTG CTTATCTTGT TTGTTAGTAA TAAGAACAGT ATCAGTAAAA 2941 AGATCCACCT TGTATTGTTT AAAAACAAGA AAACTCACAA AATTAAGCAT GTCAAACTTT 3001 ACCAGAAACA GACCCTTGAT CAGATCATTA CTAATCGTAC TGAGAATAAA TTATTTAAGA 3061 AAATTTACTC CACGATCATG TTGGTCCTTA TGTGGTTGTC TATTAGTTTT GTTATCATGT 3121 TTACATTCTG GATTGTTTTC ACGACCATTT TCAACGGCCT GTCATCTTTG AAATATTCAG 3181 AGGCTTTCTT GACTATCGAA GGGGAAGATG GAATCTTCGC CGCAATCTTA ACCACACTGC 3241 TTCTTATTTT GTGCACTCTT TTATTTGCAA TCCCGTTAGC GTTAGCATGC GCGATCTATC 3301 TGTCCGAATT CGCAAATAAA AATTCTTACT TTGCCAAATT CTTTCGTTTT CTGTTGAATT 3361 TGGCTGCATC TACGCCTTCG ATTATTTTCG GTATTTTTGG ACTGAGCGTA TTCATCATTT 3421 ATCTGAAGTT ACCGTTCTCT ATCTTCAGTG CGTCCATTAC CATGACGATT GTTGTGCTGC 3481 CAATGCTTAT CAAAAACTTT GAGGACGCCC TGACTAGCGT TCCATTGAGC TATCGTGAGG 3541 CAGCAATCGC CCTTGGTCTT AGCAAGACTA AGACCTTGTT CAAGATTGTT CTTCCGAATG 3601 CCCTTCAAGC CATTATCACA GGCACTATTC TGGCTATGGC GCGCATCATT GGTGAGTCCG 3661 CCCCGATTTA TTTGACACTT GGTACGGCGA TTAAATATCC AGACCGTGGG TTTCTTAGCA 3721 GTGGAAGTAC ATTGACAACG GGTATTTACA AAATCGCGTC GGAGTCGGCT CCAGGACAAG 3781 GGAACGACAT TGCATGGTTG ATGAGTTTGA TCACCATTAT CTTTGTCTTA ACCTTGAATT 3841 TGTCATCCAG TAAATTAAGC CTTCTGTTAG TAAAAACTAA CAAGAAAGTA AAATTTGAGT 3901 TTAAACAAAT CTATAAGAAC TTTATTAATA AGCAATTTTA CAAGACGCAA TTTAACTTAT 3961 TCAAGAATTC ACTTAAGCAA TTTATTAAAT CCCTGAAAAA GTACTTAAAT ATTACGAACC 4021 TTTTCAAGGA AATTAAGAAA ACTATTAAGT ACAAGAAAGA GTACAAAAAA TTAAAGAAAC 4081 GCGATAATAA TTACGAGTGA AGAGCTTAGA GACC //