LOCUS       Exported     2867 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..2867
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      880..927
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     2755..2771
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      2772..2803
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             1..0
                     /label="MMSYN1_0427"
                     /note="Part of the ABC transporter complex PstSACB involved in phosphate import. Responsible for energy coupling to the transport system."
                     /translation="MNNEQLITNTKPKKEPLKTAIEIKDFNFFYNKGKTQSLFNINMEIKEKSITTFIGPSGCGKTTLLKSINRLNDLIDGVKMSGVIKIFDKDIFDKDIDITKLRTEVGMVFQKPNPFPISIYDNVVYGLRSLGIKDKKILDQICEESLVKAALWDEVKDILTSPALGLSGGQQQRLCIARAIAMKPKILLMDEPTSALDPIATLKVEELVLDLKKDYTIVMVTHSLQQATRISDYTGYFLKGELVEFNKTKKIFTNPKDRRTENYISGRYE"
     primer_bind     complement(932..948)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1015..1603)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(1774..2634)
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
                     /label="bla"
     promoter        complement(2635..2739)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
     CDS             complement(1..0)
                     /translation="MNNEQLITNTKPKKEPLKTAIEIKDFNFFYNKGKTQSLFNINMEIKEKSITTFIGPSGCGKTTLLKSINRLNDLIDGVKMSGVIKIFDKDIFDKDIDITKLRTEVGMVFQKPNPFPISIYDNVVYGLRSLGIKDKKILDQICEESLVKAALWDEVKDILTSPALGLSGGQQQRLCIARAIAMKPKILLMDEPTSALDPIATLKVEELVLDLKKDYTIVMVTHSLQQATRISDYTGYFLKGELVEFNKTKKIFTNPKDRRTENYISGRYE"
                     /label="MMSYN1_0427"
                     /note="Part of the ABC transporter complex PstSACB involved in phosphate import. Responsible for energy coupling to the transport system."
ORIGIN
        1 ATGAATAATG AACAATTGAT CACTAATACC AAGCCGAAGA AAGAACCATT AAAGACTGCC
       61 ATTGAGATTA AAGATTTCAA CTTTTTCTAT AACAAGGGCA AGACCCAATC ACTGTTCAAT
      121 ATTAATATGG AGATTAAAGA AAAGTCCATT ACTACCTTTA TCGGACCTTC AGGGTGCGGC
      181 AAAACTACTT TACTTAAAAG CATCAACCGT CTGAACGACC TTATTGACGG TGTCAAGATG
      241 TCTGGCGTTA TCAAGATTTT CGATAAAGAC ATTTTCGACA AGGACATCGA TATTACAAAA
      301 TTACGCACTG AAGTGGGCAT GGTTTTCCAG AAACCCAACC CATTCCCGAT TAGCATTTAC
      361 GACAATGTCG TTTACGGTTT ACGCTCGTTG GGAATTAAGG ATAAAAAGAT CCTGGACCAA
      421 ATCTGCGAAG AATCTTTAGT CAAAGCGGCC TTGTGGGACG AGGTAAAAGA TATTTTGACT
      481 TCTCCTGCGC TGGGTTTGTC CGGGGGTCAA CAACAACGCC TTTGCATTGC ACGCGCAATT
      541 GCAATGAAGC CCAAAATCCT TCTGATGGAC GAACCTACGT CCGCTTTAGA TCCGATCGCG
      601 ACGCTTAAAG TGGAGGAGTT GGTATTGGAC CTTAAGAAGG ACTACACTAT TGTGATGGTA
      661 ACACACTCAT TGCAACAGGC AACGCGTATC TCGGATTACA CTGGGTACTT TTTGAAGGGA
      721 GAGCTTGTTG AGTTCAACAA GACAAAGAAA ATTTTCACCA ATCCGAAGGA TCGTCGCACT
      781 GAAAACTACA TCTCAGGTCG CTATGAGTGA AGAGCTTAGA GACCGGTACT AGTTCCTGCA
      841 GGGCTCGAGG TGTCAATCGT CGGAGCCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC
      901 TCTAAACGGG TCTTGAGGGG TTTTTTGCAT GGTCATAGCT GTTTCCTGAG AGCTTGGCAG
      961 GTGATGACAC ACATTAACAA ATTTCGTGAG GAGTCTCCAG AAGAATGCCA TTAATTTCCA
     1021 TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA
     1081 CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC
     1141 TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC
     1201 GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT
     1261 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG
     1321 TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG
     1381 GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA
     1441 CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG
     1501 AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT
     1561 TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT
     1621 TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG
     1681 ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT
     1741 CTAAAGTATA TATGAGTAAA CTTGGTCTGA CAGTTACCAA TGCTTAATCA GTGAGGCACC
     1801 TATCTCAGCG ATCTGTCTAT TTCGTTCATC CATAGTTGCC TGACTCCCCG TCGTGTAGAT
     1861 AACTACGATA CGGGAGGGCT TACCATCTGG CCCCAGTGCT GCAATGATAC CGCGAGAACC
     1921 ACGCTCACCG GCTCCAGATT TATCAGCAAT AAACCAGCCA GCCGGAAGGG CCGAGCGCAG
     1981 AAGTGGTCCT GCAACTTTAT CCGCCTCCAT CCAGTCTATT AATTGTTGCC GGGAAGCTAG
     2041 AGTAAGTAGT TCGCCAGTTA ATAGTTTGCG CAACGTTGTT GCCATTGCTA CAGGCATCGT
     2101 GGTGTCACGC TCGTCGTTTG GTATGGCTTC ATTCAGCTCC GGTTCCCAAC GATCAAGGCG
     2161 AGTTACATGA TCCCCCATGT TGTGCAAAAA AGCGGTTAGC TCCTTCGGTC CTCCGATCGT
     2221 TGTCAGAAGT AAGTTGGCCG CAGTGTTATC ACTCATGGTT ATGGCAGCAC TGCATAATTC
     2281 TCTTACTGTC ATGCCATCCG TAAGATGCTT TTCTGTGACT GGTGAGTACT CAACCAAGTC
     2341 ATTCTGAGAA TAGTGTATGC GGCGACCGAG TTGCTCTTGC CCGGCGTCAA TACGGGATAA
     2401 TACCGCGCCA CATAGCAGAA CTTTAAAAGT GCTCATCATT GGAAAACGTT CTTCGGGGCG
     2461 AAAACTCTCA AGGATCTTAC CGCTGTTGAG ATCCAGTTCG ATGTAACCCA CTCGTGCACC
     2521 CAACTGATCT TCAGCATCTT TTACTTTCAC CAGCGTTTCT GGGTGAGCAA AAACAGGAAG
     2581 GCAAAATGCC GCAAAAAAGG GAATAAGGGC GACACGGAAA TGTTGAATAC TCATACTCTT
     2641 CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT
     2701 TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC ACCTGCACCA GTCAGTAAAA
     2761 CGACGGCCAG TAGTCAAAAG CCTCCGACCG GAGGCTTTTG ACTTGGTCAG GTGGAGTGAA
     2821 TTCGCGGCCG CTTCTAGAGG CTCTTCGTAA TTACGAAGAG GTCTCTA
//