LOCUS       Exported     2732 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..2732
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      745..792
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
                     /label="T7 terminator"
     primer_bind     2620..2636
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      2637..2668
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             1..0
                     /label="MMSYN1_0428"
                     /note="phoU: phosphate transport system regulatory protein PhoU"
                     /translation="MSSNKILDRDLDQLRSLIENMIEETKIQYANSYLVIKEENKLIDAQKVIEHDKIINDMQNEFTSIALWKISKQKLVAKDLRLAIGGILITREIERIADYSKGISKFFIYYKPNEKHLLMISELYELVVEMLDIFSDIFENLKVDKEQQVLSLEEKINTKFRSFYDQLIDDIRQKTTKAEAEEIAAVLKQMFNLERAGDNLLNVQEIINFIKTSKFIEKTEKINK"
     primer_bind     complement(797..813)
                     /note="common sequencing primer, one of multiple similar variants"
                     /label="M13 rev"
     rep_origin      complement(880..1468)
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
                     /label="ori"
     CDS             complement(1639..2499)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(2500..2604)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
     CDS             complement(1..0)
                     /label="MMSYN1_0428"
                     /note="phoU: phosphate transport system regulatory protein PhoU"
                     /translation="MSSNKILDRDLDQLRSLIENMIEETKIQYANSYLVIKEENKLIDAQKVIEHDKIINDMQNEFTSIALWKISKQKLVAKDLRLAIGGILITREIERIADYSKGISKFFIYYKPNEKHLLMISELYELVVEMLDIFSDIFENLKVDKEQQVLSLEEKINTKFRSFYDQLIDDIRQKTTKAEAEEIAAVLKQMFNLERAGDNLLNVQEIINFIKTSKFIEKTEKINK"
ORIGIN
        1 ATGTCATCGA ACAAGATCCT TGACCGTGAT CTGGACCAGT TACGCTCTCT TATTGAAAAT
       61 ATGATTGAGG AAACTAAAAT TCAATACGCC AACTCATATC TTGTCATTAA AGAGGAGAAC
      121 AAGTTGATTG ATGCTCAGAA GGTAATCGAA CATGATAAAA TCATTAATGA CATGCAAAAC
      181 GAATTCACAA GTATTGCATT ATGGAAGATT AGTAAACAGA AGCTTGTTGC GAAAGATCTG
      241 CGTTTAGCGA TTGGCGGAAT CTTAATCACC CGCGAAATCG AACGTATCGC TGACTATAGC
      301 AAAGGGATTT CAAAGTTCTT TATCTACTAC AAGCCGAATG AGAAGCACCT TTTAATGATT
      361 TCTGAGCTTT ACGAGTTAGT TGTAGAGATG TTAGATATTT TTAGCGACAT TTTCGAGAAC
      421 TTAAAAGTCG ACAAGGAACA ACAGGTCCTG TCCTTAGAAG AGAAAATTAA TACTAAGTTT
      481 CGTAGCTTCT ACGATCAGCT TATTGATGAT ATCCGCCAAA AAACAACGAA AGCGGAAGCC
      541 GAGGAAATTG CTGCCGTATT AAAACAAATG TTCAATCTTG AACGTGCCGG AGATAACTTA
      601 TTGAACGTAC AGGAAATCAT CAATTTCATC AAGACAAGTA AATTCATTGA AAAGACGGAG
      661 AAGATCAACA AGTGAAGAGC TTAGAGACCG GTACTAGTTC CTGCAGGGCT CGAGGTGTCA
      721 ATCGTCGGAG CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG
      781 AGGGGTTTTT TGCATGGTCA TAGCTGTTTC CTGAGAGCTT GGCAGGTGAT GACACACATT
      841 AACAAATTTC GTGAGGAGTC TCCAGAAGAA TGCCATTAAT TTCCATAGGC TCCGCCCCCC
      901 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA
      961 AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC
     1021 GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC
     1081 ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA
     1141 ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC
     1201 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG
     1261 GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG
     1321 AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG
     1381 CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA
     1441 GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA
     1501 CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT
     1561 CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA
     1621 GTAAACTTGG TCTGACAGTT ACCAATGCTT AATCAGTGAG GCACCTATCT CAGCGATCTG
     1681 TCTATTTCGT TCATCCATAG TTGCCTGACT CCCCGTCGTG TAGATAACTA CGATACGGGA
     1741 GGGCTTACCA TCTGGCCCCA GTGCTGCAAT GATACCGCGA GAACCACGCT CACCGGCTCC
     1801 AGATTTATCA GCAATAAACC AGCCAGCCGG AAGGGCCGAG CGCAGAAGTG GTCCTGCAAC
     1861 TTTATCCGCC TCCATCCAGT CTATTAATTG TTGCCGGGAA GCTAGAGTAA GTAGTTCGCC
     1921 AGTTAATAGT TTGCGCAACG TTGTTGCCAT TGCTACAGGC ATCGTGGTGT CACGCTCGTC
     1981 GTTTGGTATG GCTTCATTCA GCTCCGGTTC CCAACGATCA AGGCGAGTTA CATGATCCCC
     2041 CATGTTGTGC AAAAAAGCGG TTAGCTCCTT CGGTCCTCCG ATCGTTGTCA GAAGTAAGTT
     2101 GGCCGCAGTG TTATCACTCA TGGTTATGGC AGCACTGCAT AATTCTCTTA CTGTCATGCC
     2161 ATCCGTAAGA TGCTTTTCTG TGACTGGTGA GTACTCAACC AAGTCATTCT GAGAATAGTG
     2221 TATGCGGCGA CCGAGTTGCT CTTGCCCGGC GTCAATACGG GATAATACCG CGCCACATAG
     2281 CAGAACTTTA AAAGTGCTCA TCATTGGAAA ACGTTCTTCG GGGCGAAAAC TCTCAAGGAT
     2341 CTTACCGCTG TTGAGATCCA GTTCGATGTA ACCCACTCGT GCACCCAACT GATCTTCAGC
     2401 ATCTTTTACT TTCACCAGCG TTTCTGGGTG AGCAAAAACA GGAAGGCAAA ATGCCGCAAA
     2461 AAAGGGAATA AGGGCGACAC GGAAATGTTG AATACTCATA CTCTTCCTTT TTCAATATTA
     2521 TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA
     2581 AAATAAACAA ATAGGGGTTC CGCGCACCTG CACCAGTCAG TAAAACGACG GCCAGTAGTC
     2641 AAAAGCCTCC GACCGGAGGC TTTTGACTTG GTCAGGTGGA GTGAATTCGC GGCCGCTTCT
     2701 AGAGGCTCTT CGTAATTACG AAGAGGTCTC TA
//