LOCUS Exported 2831 bp DNA circular SYN 24-JUL-2020 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2001) AUTHORS Keoni Gandall TITLE Direct Submission JOURNAL Exported from Poly. FEATURES Location/Qualifiers terminator 1..2831 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" terminator 844..891 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" primer_bind 2719..2735 /label="M13 for" /note="common sequencing primer, one of multiple similar variants" terminator 2736..2767 /label="tonB terminator" /note="bidirectional E. coli tonB-P14 transcription terminator" CDS 1..0 /label="MMSYN1_0431" /note="" /translation="MKVLMIGDVYAKPGREMLEKHLKNIVDQNQIDFIVVNGENTTHGKSICKKHYDFYKSLNVDVITSGNHIFKNAEVLEYIKTTNDLLKPLNMSKHTPGNGNVIVNKNKKKIAVVSLMGQSFMDAVNNPYDALDEFLKTNTDFDILLVDFHAESTAEKIAFAFNYDGIITAFVGTHTHVMTADERLLPNKTAFISDIGMTGVIDSIIGVEVNDVIKRAKTGLPVKFNIATGKCWLNAVIIEIDDKTNKATSIKRLTIKD" primer_bind complement(896..912) /label="M13 rev" /note="common sequencing primer, one of multiple similar variants" rep_origin complement(979..1567) /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(1738..2598) /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*" /label="bla" promoter complement(2599..2703) /label="bla promoter" /note="ampicillin resistance gene promoter" CDS complement(1..0) /label="MMSYN1_0431" /note="" /translation="MKVLMIGDVYAKPGREMLEKHLKNIVDQNQIDFIVVNGENTTHGKSICKKHYDFYKSLNVDVITSGNHIFKNAEVLEYIKTTNDLLKPLNMSKHTPGNGNVIVNKNKKKIAVVSLMGQSFMDAVNNPYDALDEFLKTNTDFDILLVDFHAESTAEKIAFAFNYDGIITAFVGTHTHVMTADERLLPNKTAFISDIGMTGVIDSIIGVEVNDVIKRAKTGLPVKFNIATGKCWLNAVIIEIDDKTNKATSIKRLTIKD" ORIGIN 1 ATGAAAGTAC TTATGATTGG TGATGTGTAC GCTAAGCCAG GCCGTGAAAT GCTTGAAAAG 61 CACCTTAAAA ATATCGTGGA TCAGAACCAG ATTGACTTTA TCGTGGTAAA CGGGGAAAAC 121 ACCACACACG GTAAATCGAT TTGCAAGAAA CATTATGATT TCTATAAATC ATTAAACGTC 181 GACGTAATCA CCAGTGGTAA CCATATCTTT AAGAACGCAG AAGTCTTGGA ATACATTAAA 241 ACAACCAACG ACCTTCTGAA ACCATTAAAC ATGTCCAAAC ACACTCCTGG CAATGGGAAT 301 GTGATTGTTA ACAAGAACAA GAAGAAAATT GCTGTCGTGT CGCTTATGGG GCAATCGTTC 361 ATGGACGCAG TAAATAATCC ATACGACGCC TTGGACGAGT TCCTGAAAAC AAATACGGAC 421 TTTGACATCC TGCTGGTCGA CTTTCACGCT GAGTCGACGG CCGAGAAGAT CGCATTTGCT 481 TTTAATTACG ACGGGATTAT CACGGCGTTT GTAGGCACGC ATACCCATGT GATGACTGCG 541 GACGAACGTT TGCTTCCTAA CAAGACAGCC TTCATTTCGG ATATTGGTAT GACAGGTGTG 601 ATCGATTCGA TTATTGGTGT AGAGGTGAAC GACGTGATCA AACGCGCCAA GACCGGGTTG 661 CCAGTGAAGT TCAATATCGC TACCGGGAAG TGCTGGTTAA ATGCCGTTAT TATCGAGATT 721 GATGACAAGA CGAATAAAGC CACATCAATT AAGCGTCTTA CTATTAAGGA TTGAAGAGCT 781 TAGAGACCGG TACTAGTTCC TGCAGGGCTC GAGGTGTCAA TCGTCGGAGC CGCTGAGCAA 841 TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCATGGTCAT 901 AGCTGTTTCC TGAGAGCTTG GCAGGTGATG ACACACATTA ACAAATTTCG TGAGGAGTCT 961 CCAGAAGAAT GCCATTAATT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG 1021 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC 1081 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC 1141 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC 1201 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG 1261 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC 1321 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA 1381 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC 1441 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC 1501 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG 1561 ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC 1621 ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA 1681 TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAAACTTGGT CTGACAGTTA 1741 CCAATGCTTA ATCAGTGAGG CACCTATCTC AGCGATCTGT CTATTTCGTT CATCCATAGT 1801 TGCCTGACTC CCCGTCGTGT AGATAACTAC GATACGGGAG GGCTTACCAT CTGGCCCCAG 1861 TGCTGCAATG ATACCGCGAG AACCACGCTC ACCGGCTCCA GATTTATCAG CAATAAACCA 1921 GCCAGCCGGA AGGGCCGAGC GCAGAAGTGG TCCTGCAACT TTATCCGCCT CCATCCAGTC 1981 TATTAATTGT TGCCGGGAAG CTAGAGTAAG TAGTTCGCCA GTTAATAGTT TGCGCAACGT 2041 TGTTGCCATT GCTACAGGCA TCGTGGTGTC ACGCTCGTCG TTTGGTATGG CTTCATTCAG 2101 CTCCGGTTCC CAACGATCAA GGCGAGTTAC ATGATCCCCC ATGTTGTGCA AAAAAGCGGT 2161 TAGCTCCTTC GGTCCTCCGA TCGTTGTCAG AAGTAAGTTG GCCGCAGTGT TATCACTCAT 2221 GGTTATGGCA GCACTGCATA ATTCTCTTAC TGTCATGCCA TCCGTAAGAT GCTTTTCTGT 2281 GACTGGTGAG TACTCAACCA AGTCATTCTG AGAATAGTGT ATGCGGCGAC CGAGTTGCTC 2341 TTGCCCGGCG TCAATACGGG ATAATACCGC GCCACATAGC AGAACTTTAA AAGTGCTCAT 2401 CATTGGAAAA CGTTCTTCGG GGCGAAAACT CTCAAGGATC TTACCGCTGT TGAGATCCAG 2461 TTCGATGTAA CCCACTCGTG CACCCAACTG ATCTTCAGCA TCTTTTACTT TCACCAGCGT 2521 TTCTGGGTGA GCAAAAACAG GAAGGCAAAA TGCCGCAAAA AAGGGAATAA GGGCGACACG 2581 GAAATGTTGA ATACTCATAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA 2641 TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC 2701 GCGCACCTGC ACCAGTCAGT AAAACGACGG CCAGTAGTCA AAAGCCTCCG ACCGGAGGCT 2761 TTTGACTTGG TCAGGTGGAG TGAATTCGCG GCCGCTTCTA GAGGCTCTTC GTAATTACGA 2821 AGAGGTCTCT A //