LOCUS       Exported     2831 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..2831
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      844..891
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     2719..2735
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      2736..2767
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             1..0
                     /label="MMSYN1_0431"
                     /note=""
                     /translation="MKVLMIGDVYAKPGREMLEKHLKNIVDQNQIDFIVVNGENTTHGKSICKKHYDFYKSLNVDVITSGNHIFKNAEVLEYIKTTNDLLKPLNMSKHTPGNGNVIVNKNKKKIAVVSLMGQSFMDAVNNPYDALDEFLKTNTDFDILLVDFHAESTAEKIAFAFNYDGIITAFVGTHTHVMTADERLLPNKTAFISDIGMTGVIDSIIGVEVNDVIKRAKTGLPVKFNIATGKCWLNAVIIEIDDKTNKATSIKRLTIKD"
     primer_bind     complement(896..912)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(979..1567)
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
                     /label="ori"
     CDS             complement(1738..2598)
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
                     /label="bla"
     promoter        complement(2599..2703)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
     CDS             complement(1..0)
                     /label="MMSYN1_0431"
                     /note=""
                     /translation="MKVLMIGDVYAKPGREMLEKHLKNIVDQNQIDFIVVNGENTTHGKSICKKHYDFYKSLNVDVITSGNHIFKNAEVLEYIKTTNDLLKPLNMSKHTPGNGNVIVNKNKKKIAVVSLMGQSFMDAVNNPYDALDEFLKTNTDFDILLVDFHAESTAEKIAFAFNYDGIITAFVGTHTHVMTADERLLPNKTAFISDIGMTGVIDSIIGVEVNDVIKRAKTGLPVKFNIATGKCWLNAVIIEIDDKTNKATSIKRLTIKD"
ORIGIN
        1 ATGAAAGTAC TTATGATTGG TGATGTGTAC GCTAAGCCAG GCCGTGAAAT GCTTGAAAAG
       61 CACCTTAAAA ATATCGTGGA TCAGAACCAG ATTGACTTTA TCGTGGTAAA CGGGGAAAAC
      121 ACCACACACG GTAAATCGAT TTGCAAGAAA CATTATGATT TCTATAAATC ATTAAACGTC
      181 GACGTAATCA CCAGTGGTAA CCATATCTTT AAGAACGCAG AAGTCTTGGA ATACATTAAA
      241 ACAACCAACG ACCTTCTGAA ACCATTAAAC ATGTCCAAAC ACACTCCTGG CAATGGGAAT
      301 GTGATTGTTA ACAAGAACAA GAAGAAAATT GCTGTCGTGT CGCTTATGGG GCAATCGTTC
      361 ATGGACGCAG TAAATAATCC ATACGACGCC TTGGACGAGT TCCTGAAAAC AAATACGGAC
      421 TTTGACATCC TGCTGGTCGA CTTTCACGCT GAGTCGACGG CCGAGAAGAT CGCATTTGCT
      481 TTTAATTACG ACGGGATTAT CACGGCGTTT GTAGGCACGC ATACCCATGT GATGACTGCG
      541 GACGAACGTT TGCTTCCTAA CAAGACAGCC TTCATTTCGG ATATTGGTAT GACAGGTGTG
      601 ATCGATTCGA TTATTGGTGT AGAGGTGAAC GACGTGATCA AACGCGCCAA GACCGGGTTG
      661 CCAGTGAAGT TCAATATCGC TACCGGGAAG TGCTGGTTAA ATGCCGTTAT TATCGAGATT
      721 GATGACAAGA CGAATAAAGC CACATCAATT AAGCGTCTTA CTATTAAGGA TTGAAGAGCT
      781 TAGAGACCGG TACTAGTTCC TGCAGGGCTC GAGGTGTCAA TCGTCGGAGC CGCTGAGCAA
      841 TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCATGGTCAT
      901 AGCTGTTTCC TGAGAGCTTG GCAGGTGATG ACACACATTA ACAAATTTCG TGAGGAGTCT
      961 CCAGAAGAAT GCCATTAATT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG
     1021 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC
     1081 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC
     1141 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC
     1201 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG
     1261 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC
     1321 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA
     1381 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC
     1441 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC
     1501 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG
     1561 ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC
     1621 ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA
     1681 TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAAACTTGGT CTGACAGTTA
     1741 CCAATGCTTA ATCAGTGAGG CACCTATCTC AGCGATCTGT CTATTTCGTT CATCCATAGT
     1801 TGCCTGACTC CCCGTCGTGT AGATAACTAC GATACGGGAG GGCTTACCAT CTGGCCCCAG
     1861 TGCTGCAATG ATACCGCGAG AACCACGCTC ACCGGCTCCA GATTTATCAG CAATAAACCA
     1921 GCCAGCCGGA AGGGCCGAGC GCAGAAGTGG TCCTGCAACT TTATCCGCCT CCATCCAGTC
     1981 TATTAATTGT TGCCGGGAAG CTAGAGTAAG TAGTTCGCCA GTTAATAGTT TGCGCAACGT
     2041 TGTTGCCATT GCTACAGGCA TCGTGGTGTC ACGCTCGTCG TTTGGTATGG CTTCATTCAG
     2101 CTCCGGTTCC CAACGATCAA GGCGAGTTAC ATGATCCCCC ATGTTGTGCA AAAAAGCGGT
     2161 TAGCTCCTTC GGTCCTCCGA TCGTTGTCAG AAGTAAGTTG GCCGCAGTGT TATCACTCAT
     2221 GGTTATGGCA GCACTGCATA ATTCTCTTAC TGTCATGCCA TCCGTAAGAT GCTTTTCTGT
     2281 GACTGGTGAG TACTCAACCA AGTCATTCTG AGAATAGTGT ATGCGGCGAC CGAGTTGCTC
     2341 TTGCCCGGCG TCAATACGGG ATAATACCGC GCCACATAGC AGAACTTTAA AAGTGCTCAT
     2401 CATTGGAAAA CGTTCTTCGG GGCGAAAACT CTCAAGGATC TTACCGCTGT TGAGATCCAG
     2461 TTCGATGTAA CCCACTCGTG CACCCAACTG ATCTTCAGCA TCTTTTACTT TCACCAGCGT
     2521 TTCTGGGTGA GCAAAAACAG GAAGGCAAAA TGCCGCAAAA AAGGGAATAA GGGCGACACG
     2581 GAAATGTTGA ATACTCATAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA
     2641 TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC
     2701 GCGCACCTGC ACCAGTCAGT AAAACGACGG CCAGTAGTCA AAAGCCTCCG ACCGGAGGCT
     2761 TTTGACTTGG TCAGGTGGAG TGAATTCGCG GCCGCTTCTA GAGGCTCTTC GTAATTACGA
     2821 AGAGGTCTCT A
//