LOCUS Exported 3340 bp DNA circular SYN 24-JUL-2020 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2001) AUTHORS Keoni Gandall TITLE Direct Submission JOURNAL Exported from Poly. FEATURES Location/Qualifiers terminator 1..3340 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" terminator 38..85 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" primer_bind 1933..1949 /label="M13 for" /note="common sequencing primer, one of multiple similar variants" terminator 1950..1981 /label="tonB terminator" /note="bidirectional E. coli tonB-P14 transcription terminator" CDS 2010..3326 /label="MMSYN1_0434" /note="Catalyzes the folate-dependent formation of 5-methyl-uridine at position 54 (M-5-U54) in all tRNAs." /translation="MNKKVKIIGAGLAGCEAAYFLANNNIQVELYEVKTLIKNEVQKTNNFAELVCSNTFRSQSLLNAAGILKAEMRRLNSLVIKIADSCKIDGDDALAVDREDFSKKLTEVIKNHPNITIIEQNVSHIDDENDLTLIATGPLTTNELKEDIQRLIGKQKLFFMDASAPIITKDSIDFNKAYYSGRHKLGKYICCPLNEQEFNEFADNLINAEQVQLKEFEKSIFFKGCQPIEQLAKTSKKLLLKGPMSSNNLLDQNNHQPYAVVQLRQDDAKDSLYNMVGFQTNLKWPEQKRVFQTIPGLQKAKIVRYGVMHKNYYINSPKILNFKLQVMRKKNVFFAGQITGVEGYIESASSGIWAAINILAFINNKKLKPLPNTTILGALTNYITNSKIYSLKPMKCNLGILEQENKYQSDDKFYSFNNSKNSLEEYIKQLNQILDTSI" primer_bind complement(90..106) /label="M13 rev" /note="common sequencing primer, one of multiple similar variants" rep_origin complement(173..761) /label="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(952..1812) /label="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*" promoter complement(1813..1917) /label="bla promoter" /note="ampicillin resistance gene promoter" ORIGIN 1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC 61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT 121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA 181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC 241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG 301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC 361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG 421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC 481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA 541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG 601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA 661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG 721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA 781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA 841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA 901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG 961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG 1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC 1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC 1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA 1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC 1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG 1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC 1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT 1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG 1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC 1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG 1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT 1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG 1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG 1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT 1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC 1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC 1981 TTGGTTCAGG TGGAGTGGGA GGGTCTCAAA TGAACAAAAA AGTTAAAATC ATCGGTGCAG 2041 GATTAGCCGG CTGCGAAGCT GCGTACTTCT TAGCTAATAA TAATATCCAA GTGGAGTTAT 2101 ATGAGGTTAA GACCTTAATC AAGAACGAGG TGCAGAAAAC CAATAACTTC GCTGAGCTGG 2161 TGTGCAGTAA CACTTTTCGT TCACAGTCAT TATTGAATGC TGCCGGGATT CTTAAGGCAG 2221 AAATGCGTCG TTTGAACTCC TTAGTGATTA AAATCGCAGA CTCATGTAAA ATTGACGGCG 2281 ACGATGCGCT TGCAGTTGAT CGCGAGGACT TTAGCAAAAA GCTTACAGAA GTAATTAAAA 2341 ACCACCCGAA CATTACTATT ATTGAACAGA ATGTATCTCA CATTGACGAT GAAAACGATC 2401 TGACTTTGAT TGCTACAGGA CCACTTACGA CTAACGAGTT GAAGGAGGAT ATCCAACGTC 2461 TGATCGGGAA ACAGAAGTTA TTTTTCATGG ACGCGAGTGC TCCGATCATT ACAAAAGATT 2521 CCATCGATTT CAATAAAGCT TATTACAGCG GACGCCATAA ACTGGGTAAG TATATCTGCT 2581 GTCCGTTGAA TGAGCAGGAG TTCAACGAAT TCGCGGACAA CCTTATCAAT GCTGAACAGG 2641 TCCAGTTGAA GGAGTTCGAG AAATCTATCT TCTTCAAGGG CTGTCAACCC ATTGAACAGT 2701 TAGCTAAGAC GAGCAAAAAA CTGTTATTGA AGGGGCCAAT GTCTAGTAAC AATTTATTGG 2761 ACCAAAACAA TCACCAGCCT TACGCAGTGG TCCAGTTACG CCAAGACGAT GCCAAGGATT 2821 CATTATACAA CATGGTAGGC TTCCAGACCA ACCTGAAATG GCCAGAGCAA AAACGTGTGT 2881 TTCAAACTAT TCCGGGCTTG CAGAAAGCTA AAATTGTGCG CTATGGCGTA ATGCATAAGA 2941 ATTATTATAT TAACAGTCCT AAGATCCTTA ATTTTAAGCT CCAGGTCATG CGTAAGAAAA 3001 ACGTATTCTT TGCTGGCCAA ATCACTGGTG TAGAGGGGTA CATTGAGAGT GCCAGCTCAG 3061 GGATTTGGGC AGCGATTAAC ATCCTGGCCT TTATTAACAA CAAGAAATTG AAGCCACTTC 3121 CCAACACAAC CATTCTGGGT GCGCTGACGA ACTACATTAC TAACAGCAAG ATTTATTCCC 3181 TTAAACCCAT GAAATGCAAT CTTGGCATTC TGGAGCAAGA AAATAAGTAT CAAAGTGACG 3241 ACAAATTTTA CTCGTTCAAC AACTCCAAAA ACAGTTTGGA GGAGTACATT AAGCAGTTGA 3301 ATCAGATCCT TGATACTTCT ATTTGAAGAG CTTAGAGACC //