LOCUS Exported 4180 bp DNA circular SYN 24-JUL-2020 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2001) AUTHORS Keoni Gandall TITLE Direct Submission JOURNAL Exported from Poly. FEATURES Location/Qualifiers terminator 1..4180 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" terminator 38..85 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" primer_bind 1933..1949 /note="common sequencing primer, one of multiple similar variants" /label="M13 for" terminator 1950..1981 /label="tonB terminator" /note="bidirectional E. coli tonB-P14 transcription terminator" CDS 2010..4166 /label="MMSYN1_0439" /note="lipoprotein, putative" /translation="MKKLLTILGSILLSAGTTTVAVACTTKNDKFDKPSITDELSQKIISGLKLSDDFNFTTGERFSKLDYKSLILDMINETISKNKYTDNLNNLSKKFGLEIKQTKELGDKKAEEVLKNLSTIKLFADYTSKRASEENSDSIDLSYSENYPLNPYNLESKNGQKDRTVYAIYYKNNNNTSSSGSSSNGGGSNGGTTWLRWQTTGEFDTLSSTIPSTPQLPSVSLLTDTSTKNFRIAKLSKPTEQDYITKTASVNDDGKATNNGGNESVEWYKNSNDKFETDGQGIMQYRFMYHFKTKIEAKLFNDLLGHAYIDSNLFVDKNDNKSASNKKIILNNVSKLISDIQSNYSQVDKTISNVKMVWAFSLDKQKVSEVNAEINQYVNPDGSLINKDNKKTLKNVFDKIKSKTNNESKQGTDSLLSISGFNGFVKNKDNNIESLSGDLKITEEAKKAVARVNAPSLLTNNNNGFTSENSNNVDYVFVLPIYLNDLFSSNDMQIKRNTGSNGGAGSNGSNYELNVMQNTWVNLNDKFSLDNRYFDNLTIKKVESKDNGEALVANNNDKWYVSLKNGSDSKKVEVTYSDNSKKMITLKKADPNNIKTLDFTYKLSNSDFNKQLFKDKLKDSFISYDINLKNYDNIKDKQNDAYIWNNDPKKSNDIQELSAAKKQVLLDQLEAITAKNPDVQNAAKTELYSAYLYTDGIYYKSLFDEISKYIESEKPTLD" primer_bind complement(90..106) /label="M13 rev" /note="common sequencing primer, one of multiple similar variants" rep_origin complement(173..761) /label="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(952..1812) /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*" /label="bla" promoter complement(1813..1917) /note="ampicillin resistance gene promoter" /label="bla promoter" ORIGIN 1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC 61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT 121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA 181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC 241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG 301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC 361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG 421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC 481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA 541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG 601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA 661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG 721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA 781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA 841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA 901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG 961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG 1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC 1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC 1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA 1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC 1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG 1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC 1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT 1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG 1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC 1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG 1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT 1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG 1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG 1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT 1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC 1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC 1981 TTGGTTCAGG TGGAGTGGGA GGGTCTCAAA TGAAGAAGTT ATTAACCATT TTAGGAAGCA 2041 TCTTGCTTTC AGCGGGTACG ACAACAGTGG CTGTGGCCTG CACAACGAAA AATGATAAGT 2101 TCGACAAGCC TTCTATTACT GACGAATTAT CACAAAAGAT TATTAGTGGG TTAAAGCTGT 2161 CCGACGATTT TAACTTCACT ACCGGTGAGC GTTTCTCCAA GCTGGACTAC AAGAGTCTTA 2221 TCCTTGATAT GATCAACGAA ACGATTTCGA AGAATAAGTA TACAGATAAC CTTAACAACC 2281 TTTCGAAAAA GTTTGGGTTG GAGATCAAAC AAACCAAGGA ATTAGGCGAC AAGAAAGCTG 2341 AAGAAGTGCT GAAAAACTTG TCTACCATTA AGCTGTTTGC CGATTATACT TCTAAACGTG 2401 CATCTGAAGA GAATTCAGAT TCCATCGACT TGAGTTATTC AGAAAATTAT CCCTTAAACC 2461 CTTACAATCT TGAGAGTAAG AACGGGCAAA AAGACCGCAC AGTTTATGCT ATTTACTACA 2521 AAAATAATAA CAACACCTCT AGCTCAGGAT CTTCTTCAAA CGGCGGAGGC AGTAACGGGG 2581 GAACAACATG GTTACGTTGG CAGACCACAG GAGAATTTGA TACCCTTAGC TCGACGATCC 2641 CCTCTACACC TCAATTGCCT TCAGTCAGTC TGCTGACCGA CACGTCGACT AAGAACTTCC 2701 GTATTGCTAA GCTTAGCAAA CCGACAGAAC AAGACTATAT TACAAAGACA GCATCAGTAA 2761 ACGACGACGG TAAGGCAACC AATAATGGGG GCAACGAATC TGTAGAGTGG TATAAAAATA 2821 GCAACGATAA ATTTGAAACA GACGGACAAG GTATTATGCA GTATCGCTTT ATGTATCATT 2881 TCAAGACCAA AATTGAAGCT AAATTGTTTA ATGATCTGCT TGGACATGCC TACATTGATA 2941 GTAACCTTTT TGTCGACAAA AATGATAATA AAAGTGCCTC GAATAAGAAA ATTATCCTTA 3001 ACAATGTGTC AAAGTTGATC TCAGACATTC AATCTAATTA CAGTCAAGTA GACAAAACGA 3061 TTAGCAATGT AAAGATGGTT TGGGCCTTTT CCCTTGACAA GCAAAAGGTG AGTGAAGTAA 3121 ACGCTGAAAT TAATCAATAC GTTAATCCCG ACGGCTCCTT GATCAACAAG GATAATAAGA 3181 AAACCCTGAA AAACGTGTTT GATAAAATCA AGAGCAAGAC TAACAACGAG TCTAAACAGG 3241 GAACAGATTC ACTTTTGTCC ATTTCTGGTT TTAATGGCTT CGTCAAGAAT AAAGACAATA 3301 ACATTGAAAG CTTATCGGGA GATCTGAAAA TCACAGAGGA AGCTAAAAAG GCAGTTGCTC 3361 GCGTAAATGC TCCTAGTCTG TTGACCAACA ATAATAACGG ATTTACTAGT GAAAATAGTA 3421 ATAACGTGGA TTACGTTTTT GTACTGCCGA TTTATTTGAA TGACTTGTTC AGTAGTAATG 3481 ATATGCAGAT CAAGCGTAAT ACGGGCTCGA ATGGAGGAGC AGGGTCTAAT GGTAGCAACT 3541 ATGAATTGAA TGTTATGCAG AACACATGGG TGAATTTGAA CGACAAGTTC TCGTTAGACA 3601 ACCGTTACTT CGATAACTTA ACAATCAAGA AGGTGGAAAG TAAAGACAAC GGTGAAGCCC 3661 TTGTCGCCAA TAACAACGAT AAGTGGTATG TCAGTCTTAA AAATGGGAGT GACTCCAAGA 3721 AAGTGGAAGT GACATACTCA GATAATTCTA AGAAAATGAT CACACTTAAA AAAGCAGATC 3781 CGAATAATAT CAAAACCCTG GATTTCACTT ATAAGTTGTC GAACTCTGAT TTTAATAAAC 3841 AACTGTTCAA AGACAAATTG AAGGACTCCT TTATTAGCTA CGACATTAAC TTGAAGAATT 3901 ACGATAACAT TAAGGACAAG CAAAATGATG CATATATTTG GAACAACGAT CCTAAAAAGA 3961 GTAACGACAT CCAGGAGTTA AGTGCAGCTA AAAAACAGGT GTTGCTGGAC CAGCTTGAGG 4021 CCATCACCGC CAAAAACCCC GACGTTCAAA ACGCGGCCAA AACCGAACTT TATTCGGCAT 4081 ATTTATATAC AGACGGGATC TATTACAAAT CGTTGTTCGA TGAGATCAGC AAATACATCG 4141 AGTCTGAAAA GCCAACGCTG GATTGAAGAG CTTAGAGACC //