LOCUS       Exported     4180 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..4180
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      38..85
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     1933..1949
                     /note="common sequencing primer, one of multiple similar variants"
                     /label="M13 for"
     terminator      1950..1981
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             2010..4166
                     /label="MMSYN1_0439"
                     /note="lipoprotein, putative"
                     /translation="MKKLLTILGSILLSAGTTTVAVACTTKNDKFDKPSITDELSQKIISGLKLSDDFNFTTGERFSKLDYKSLILDMINETISKNKYTDNLNNLSKKFGLEIKQTKELGDKKAEEVLKNLSTIKLFADYTSKRASEENSDSIDLSYSENYPLNPYNLESKNGQKDRTVYAIYYKNNNNTSSSGSSSNGGGSNGGTTWLRWQTTGEFDTLSSTIPSTPQLPSVSLLTDTSTKNFRIAKLSKPTEQDYITKTASVNDDGKATNNGGNESVEWYKNSNDKFETDGQGIMQYRFMYHFKTKIEAKLFNDLLGHAYIDSNLFVDKNDNKSASNKKIILNNVSKLISDIQSNYSQVDKTISNVKMVWAFSLDKQKVSEVNAEINQYVNPDGSLINKDNKKTLKNVFDKIKSKTNNESKQGTDSLLSISGFNGFVKNKDNNIESLSGDLKITEEAKKAVARVNAPSLLTNNNNGFTSENSNNVDYVFVLPIYLNDLFSSNDMQIKRNTGSNGGAGSNGSNYELNVMQNTWVNLNDKFSLDNRYFDNLTIKKVESKDNGEALVANNNDKWYVSLKNGSDSKKVEVTYSDNSKKMITLKKADPNNIKTLDFTYKLSNSDFNKQLFKDKLKDSFISYDINLKNYDNIKDKQNDAYIWNNDPKKSNDIQELSAAKKQVLLDQLEAITAKNPDVQNAAKTELYSAYLYTDGIYYKSLFDEISKYIESEKPTLD"
     primer_bind     complement(90..106)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(173..761)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(952..1812)
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
                     /label="bla"
     promoter        complement(1813..1917)
                     /note="ampicillin resistance gene promoter"
                     /label="bla promoter"
ORIGIN
        1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC
       61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT
      121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA
      181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC
      241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG
      301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC
      361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG
      421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC
      481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA
      541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG
      601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA
      661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG
      721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA
      781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA
      841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA
      901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG
      961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG
     1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC
     1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC
     1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA
     1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC
     1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG
     1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC
     1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT
     1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG
     1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC
     1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG
     1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT
     1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG
     1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG
     1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT
     1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC
     1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC
     1981 TTGGTTCAGG TGGAGTGGGA GGGTCTCAAA TGAAGAAGTT ATTAACCATT TTAGGAAGCA
     2041 TCTTGCTTTC AGCGGGTACG ACAACAGTGG CTGTGGCCTG CACAACGAAA AATGATAAGT
     2101 TCGACAAGCC TTCTATTACT GACGAATTAT CACAAAAGAT TATTAGTGGG TTAAAGCTGT
     2161 CCGACGATTT TAACTTCACT ACCGGTGAGC GTTTCTCCAA GCTGGACTAC AAGAGTCTTA
     2221 TCCTTGATAT GATCAACGAA ACGATTTCGA AGAATAAGTA TACAGATAAC CTTAACAACC
     2281 TTTCGAAAAA GTTTGGGTTG GAGATCAAAC AAACCAAGGA ATTAGGCGAC AAGAAAGCTG
     2341 AAGAAGTGCT GAAAAACTTG TCTACCATTA AGCTGTTTGC CGATTATACT TCTAAACGTG
     2401 CATCTGAAGA GAATTCAGAT TCCATCGACT TGAGTTATTC AGAAAATTAT CCCTTAAACC
     2461 CTTACAATCT TGAGAGTAAG AACGGGCAAA AAGACCGCAC AGTTTATGCT ATTTACTACA
     2521 AAAATAATAA CAACACCTCT AGCTCAGGAT CTTCTTCAAA CGGCGGAGGC AGTAACGGGG
     2581 GAACAACATG GTTACGTTGG CAGACCACAG GAGAATTTGA TACCCTTAGC TCGACGATCC
     2641 CCTCTACACC TCAATTGCCT TCAGTCAGTC TGCTGACCGA CACGTCGACT AAGAACTTCC
     2701 GTATTGCTAA GCTTAGCAAA CCGACAGAAC AAGACTATAT TACAAAGACA GCATCAGTAA
     2761 ACGACGACGG TAAGGCAACC AATAATGGGG GCAACGAATC TGTAGAGTGG TATAAAAATA
     2821 GCAACGATAA ATTTGAAACA GACGGACAAG GTATTATGCA GTATCGCTTT ATGTATCATT
     2881 TCAAGACCAA AATTGAAGCT AAATTGTTTA ATGATCTGCT TGGACATGCC TACATTGATA
     2941 GTAACCTTTT TGTCGACAAA AATGATAATA AAAGTGCCTC GAATAAGAAA ATTATCCTTA
     3001 ACAATGTGTC AAAGTTGATC TCAGACATTC AATCTAATTA CAGTCAAGTA GACAAAACGA
     3061 TTAGCAATGT AAAGATGGTT TGGGCCTTTT CCCTTGACAA GCAAAAGGTG AGTGAAGTAA
     3121 ACGCTGAAAT TAATCAATAC GTTAATCCCG ACGGCTCCTT GATCAACAAG GATAATAAGA
     3181 AAACCCTGAA AAACGTGTTT GATAAAATCA AGAGCAAGAC TAACAACGAG TCTAAACAGG
     3241 GAACAGATTC ACTTTTGTCC ATTTCTGGTT TTAATGGCTT CGTCAAGAAT AAAGACAATA
     3301 ACATTGAAAG CTTATCGGGA GATCTGAAAA TCACAGAGGA AGCTAAAAAG GCAGTTGCTC
     3361 GCGTAAATGC TCCTAGTCTG TTGACCAACA ATAATAACGG ATTTACTAGT GAAAATAGTA
     3421 ATAACGTGGA TTACGTTTTT GTACTGCCGA TTTATTTGAA TGACTTGTTC AGTAGTAATG
     3481 ATATGCAGAT CAAGCGTAAT ACGGGCTCGA ATGGAGGAGC AGGGTCTAAT GGTAGCAACT
     3541 ATGAATTGAA TGTTATGCAG AACACATGGG TGAATTTGAA CGACAAGTTC TCGTTAGACA
     3601 ACCGTTACTT CGATAACTTA ACAATCAAGA AGGTGGAAAG TAAAGACAAC GGTGAAGCCC
     3661 TTGTCGCCAA TAACAACGAT AAGTGGTATG TCAGTCTTAA AAATGGGAGT GACTCCAAGA
     3721 AAGTGGAAGT GACATACTCA GATAATTCTA AGAAAATGAT CACACTTAAA AAAGCAGATC
     3781 CGAATAATAT CAAAACCCTG GATTTCACTT ATAAGTTGTC GAACTCTGAT TTTAATAAAC
     3841 AACTGTTCAA AGACAAATTG AAGGACTCCT TTATTAGCTA CGACATTAAC TTGAAGAATT
     3901 ACGATAACAT TAAGGACAAG CAAAATGATG CATATATTTG GAACAACGAT CCTAAAAAGA
     3961 GTAACGACAT CCAGGAGTTA AGTGCAGCTA AAAAACAGGT GTTGCTGGAC CAGCTTGAGG
     4021 CCATCACCGC CAAAAACCCC GACGTTCAAA ACGCGGCCAA AACCGAACTT TATTCGGCAT
     4081 ATTTATATAC AGACGGGATC TATTACAAAT CGTTGTTCGA TGAGATCAGC AAATACATCG
     4141 AGTCTGAAAA GCCAACGCTG GATTGAAGAG CTTAGAGACC
//