LOCUS       Exported     3262 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3262
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      38..85
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     1933..1949
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      1950..1981
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
                     /label="tonB terminator"
     CDS             2010..3248
                     /label="MMSYN1_0441"
                     /note="IscS: Cysteine desulfurase"
                     /translation="MNDQFEKIKKQFPLLKKHPNLIYFDNGATTLKPNSVINAQTNYLKNISTNPHSSDYKIGYQSLEILSNTRELVKNFINANHTSEIIFTSGTTQSINMIAKGLINLINQDDEILITSLEHSSNLVPWIWLKQKTNAVIKNLELTNDFGIDINKLDQLITPKTKIISFAHISNTTGYINDVKKIIQKIRSINQNVIIVVDVAQSIAHFKVDVKDWDVDFIAFSAHKMYGPFGVGVLYGKYQLLDKLEPLNLGGGSSLTISRDFTSYTLKSLPEKLEAGTLNISNIYGFKKAIEFILKIGINNICLYETKLKQYTRQQIKANHLENKITFYNLNNDSPLLLFNVNQINAQDISSFLDVKYNITSRSGAHCVRRLEDVIHIKSALRISFAIYNTTDEIDKLIDALKNTDKFLDIYF"
     primer_bind     complement(90..106)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(173..761)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(952..1812)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(1813..1917)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC
       61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT
      121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA
      181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC
      241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG
      301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC
      361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG
      421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC
      481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA
      541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG
      601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA
      661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG
      721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA
      781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA
      841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA
      901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG
      961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG
     1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC
     1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC
     1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA
     1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC
     1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG
     1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC
     1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT
     1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG
     1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC
     1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG
     1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT
     1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG
     1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG
     1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT
     1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC
     1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC
     1981 TTGGTTCAGG TGGAGTGGGA GGGTCTCAAA TGAATGATCA GTTCGAAAAA ATTAAAAAAC
     2041 AGTTTCCTCT TTTGAAGAAA CACCCCAACC TGATTTACTT TGACAACGGA GCAACAACGC
     2101 TGAAACCAAA CTCGGTGATC AACGCGCAGA CCAACTACTT GAAGAACATC TCCACCAATC
     2161 CTCACAGCAG TGACTACAAA ATTGGATACC AGAGTTTAGA GATTCTGTCC AATACTCGCG
     2221 AGCTTGTCAA GAACTTCATC AACGCCAACC ACACTTCTGA AATTATCTTT ACAAGTGGTA
     2281 CAACTCAAAG TATCAATATG ATCGCCAAGG GTTTGATTAA TTTGATTAAT CAGGATGATG
     2341 AAATCCTTAT TACAAGTCTT GAACACTCTT CTAATTTAGT TCCATGGATT TGGCTGAAGC
     2401 AAAAGACAAA TGCTGTAATT AAAAATCTTG AGCTGACTAA CGATTTTGGA ATCGACATCA
     2461 ATAAGTTAGA CCAATTAATT ACACCGAAAA CGAAGATTAT CTCATTCGCT CATATCAGCA
     2521 ACACAACTGG GTATATCAAC GACGTTAAAA AGATCATCCA GAAAATCCGC TCGATCAACC
     2581 AAAATGTGAT TATTGTAGTG GATGTAGCCC AGAGTATCGC CCACTTCAAA GTTGACGTTA
     2641 AAGATTGGGA CGTAGACTTT ATCGCCTTCT CAGCACATAA AATGTACGGG CCTTTTGGTG
     2701 TTGGTGTACT TTACGGGAAG TATCAGTTAT TGGACAAGTT AGAACCTCTG AACTTAGGAG
     2761 GAGGTTCTTC ACTGACAATT TCTCGCGATT TTACAAGCTA TACCCTTAAA TCACTTCCCG
     2821 AAAAATTAGA GGCTGGAACT CTTAACATTT CAAACATTTA CGGATTTAAA AAGGCTATTG
     2881 AGTTCATTCT GAAAATCGGG ATCAACAACA TCTGTTTATA TGAAACGAAA CTTAAGCAAT
     2941 ATACGCGTCA ACAAATCAAG GCGAATCATT TAGAGAATAA GATTACTTTT TATAATCTGA
     3001 ATAACGACAG TCCTCTTCTT TTGTTCAACG TAAATCAGAT TAATGCCCAA GATATTTCTA
     3061 GCTTCCTGGA TGTGAAATAC AATATTACTT CGCGTTCGGG TGCTCATTGT GTGCGTCGTT
     3121 TGGAGGACGT GATTCACATC AAATCCGCGT TGCGTATCTC TTTCGCGATT TATAATACCA
     3181 CAGATGAGAT CGACAAATTA ATCGACGCTT TAAAGAATAC GGATAAATTT CTTGATATTT
     3241 ATTTTTGAAG AGCTTAGAGA CC
//