LOCUS       Exported     3919 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3919
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      38..85
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     1933..1949
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      1950..1981
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             2010..3905
                     /label="MMSYN1_0444"
                     /note="peptidase family M13"
                     /translation="MKYQIKDNLFKAVNQDWLEKTEIPNDRSSIGEFVELDIKNELIIKKIAKDLLKKQANNLLDDPNLINFAKFYSLTSNFELRNKNHIEPLKKYVNEILEIKNLDQLNQMYTTFVYRNYSLPINFDISNDYIDSSIKTLYLTIASHILPDKSHYQNKEVKNKFYKEFKAMTKKLLSAYFNDVKKINLIIKNTLEFDEIIANYSLSSLEKVRYNELYKPYKYEDVIKNTKYLDLNNIIKTLINKDVDQIIFTDDHFATNLDQIYNNKNLELIKSWLVVMLVVRFSKYLDEKTRTTASKYSLFISGQTKVKNKEKHALNLALDYFSTPIGLYYGQKYLGSKAKKDVENMVSHMINIYKQRLKNNTWLTSQTINKALLKLDKLGVHIGYPSEIEPFYANLITNSTNLIDTVFNFNQVINQYLFSEYKKPINKNYWSMAAYQVNAYYHPMYNHIVFPAGILQGSFYSINHSTSQNYGGIGAVIAHEISHAFDNNGANFDENGNLKMWWTDEDFDKFKQKTQKMIDLFDNKEIEFGKCNGTLTVSENIADAGGISCALQAAKLEKDYNAQEFFINWAKIWKSKYKQQTALRLLETDPHAPTELRANIQAANLEEFVDAFNINPEDKMYIDPQKRVKIW"
     primer_bind     complement(90..106)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(173..761)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(952..1812)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(1813..1917)
                     /note="ampicillin resistance gene promoter"
                     /label="bla promoter"
ORIGIN
        1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC
       61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT
      121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA
      181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC
      241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG
      301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC
      361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG
      421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC
      481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA
      541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG
      601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA
      661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG
      721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA
      781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA
      841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA
      901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG
      961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG
     1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC
     1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC
     1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA
     1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC
     1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG
     1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC
     1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT
     1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG
     1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC
     1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG
     1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT
     1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG
     1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG
     1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT
     1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC
     1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC
     1981 TTGGTTCAGG TGGAGTGGGA GGGTCTCAAA TGAAGTATCA AATTAAAGAC AACCTTTTCA
     2041 AGGCGGTAAA TCAAGACTGG TTGGAAAAGA CTGAGATTCC AAATGATCGC AGTAGTATTG
     2101 GTGAGTTTGT AGAACTTGAC ATTAAAAACG AATTAATCAT TAAGAAGATC GCGAAAGATT
     2161 TACTGAAAAA GCAGGCGAAC AATTTACTGG ATGATCCTAA TTTAATTAAT TTCGCAAAGT
     2221 TCTATAGTTT GACCTCGAAT TTTGAACTGC GTAACAAAAA CCACATTGAA CCTTTGAAGA
     2281 AATATGTGAA TGAAATTCTT GAAATCAAAA ATTTGGACCA GTTGAACCAA ATGTACACCA
     2341 CGTTTGTCTA TCGTAATTAC AGCCTTCCGA TCAATTTTGA TATTAGTAAC GACTATATCG
     2401 ATTCTTCAAT CAAGACCTTG TACTTGACGA TTGCTAGTCA CATCCTGCCT GACAAATCAC
     2461 ACTACCAGAA TAAGGAGGTG AAAAACAAAT TTTATAAAGA ATTCAAGGCT ATGACTAAGA
     2521 AATTGTTGAG TGCATACTTT AATGATGTTA AGAAAATTAA TCTTATCATC AAGAACACTC
     2581 TTGAATTCGA TGAAATTATC GCCAATTATA GCTTAAGCTC CCTGGAGAAA GTCCGCTACA
     2641 ATGAACTTTA CAAGCCCTAC AAATATGAGG ACGTGATCAA AAATACAAAG TATCTGGATC
     2701 TGAATAATAT CATTAAGACG TTAATCAATA AAGATGTCGA TCAAATTATT TTTACTGATG
     2761 ACCATTTTGC AACTAATTTG GATCAGATTT ATAACAACAA GAATCTGGAA CTTATCAAGA
     2821 GTTGGTTGGT GGTAATGTTG GTTGTGCGCT TTTCTAAATA CTTGGACGAA AAAACGCGCA
     2881 CGACGGCGTC TAAGTATTCC CTTTTCATCA GCGGACAGAC TAAGGTTAAG AACAAGGAGA
     2941 AACATGCTCT GAATTTAGCA TTGGATTACT TCAGCACTCC TATCGGTCTG TACTATGGGC
     3001 AAAAGTACTT AGGCTCCAAA GCAAAGAAAG ATGTTGAGAA TATGGTTTCA CACATGATCA
     3061 ACATTTACAA ACAGCGTCTT AAGAACAATA CGTGGCTTAC ATCCCAAACT ATTAACAAGG
     3121 CGCTTCTTAA GTTAGATAAA CTGGGAGTTC ACATTGGATA CCCTAGTGAG ATTGAACCGT
     3181 TTTATGCAAA CCTTATTACT AATAGTACCA ATCTGATCGA CACGGTTTTT AATTTCAATC
     3241 AAGTAATTAA TCAATATTTA TTCAGTGAAT ACAAAAAACC GATCAATAAA AATTATTGGT
     3301 CTATGGCCGC CTACCAAGTG AACGCCTACT ATCATCCTAT GTACAACCAC ATCGTTTTCC
     3361 CCGCAGGCAT TTTGCAGGGC TCTTTTTACT CCATCAATCA CAGCACATCA CAGAACTATG
     3421 GCGGTATCGG AGCGGTTATC GCACACGAAA TCTCCCATGC CTTTGATAAC AACGGGGCCA
     3481 ATTTTGACGA AAATGGCAAC CTTAAGATGT GGTGGACCGA TGAGGACTTC GACAAATTCA
     3541 AACAGAAAAC ACAGAAAATG ATTGATTTGT TTGATAACAA AGAAATCGAA TTTGGAAAGT
     3601 GCAACGGCAC TTTAACTGTC AGCGAGAATA TTGCTGATGC TGGGGGCATT TCGTGCGCTT
     3661 TGCAAGCCGC TAAACTTGAG AAAGACTACA ACGCACAAGA ATTTTTTATT AATTGGGCCA
     3721 AAATCTGGAA ATCAAAGTAT AAACAGCAGA CGGCGCTTCG TTTATTGGAA ACGGATCCAC
     3781 ACGCCCCGAC AGAGTTGCGT GCGAATATCC AAGCAGCAAA TTTAGAAGAA TTTGTTGACG
     3841 CATTCAATAT CAATCCCGAG GATAAGATGT ACATTGATCC GCAGAAGCGC GTTAAGATCT
     3901 GGTGAAGAGC TTAGAGACC
//