LOCUS       Exported     2825 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..2825
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      838..885
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     2713..2729
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      2730..2761
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             1..0
                     /label="MMSYN1_0448"
                     /note="trmH-like"
                     /translation="MEVISSVSNPKIKEILKLKDRKHRNKQKLFIVEGFHMIMEAYNDQIIKTLLGTSKALEILKDEIPNIEQVIEISENVAKKISDTVTSQQIFAICSMPENTKIDFENNILLLDQIQDPGNLGTLIRSAASFNFKTVIASPNSVNFHNQKVLRSTQGNLFQVNLVNEYLVTVINQLHDNNYIIIGTSLHDDSKPLSKVKFDSDDKYALIIGNEGKGISPELLDLIDLNINIEMAEDVDSINAAVAGSIIMYQINNAK"
     primer_bind     complement(890..906)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(973..1561)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(1732..2592)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(2593..2697)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
     CDS             complement(1..0)
                     /label="MMSYN1_0448"
                     /note="trmH-like"
                     /translation="MEVISSVSNPKIKEILKLKDRKHRNKQKLFIVEGFHMIMEAYNDQIIKTLLGTSKALEILKDEIPNIEQVIEISENVAKKISDTVTSQQIFAICSMPENTKIDFENNILLLDQIQDPGNLGTLIRSAASFNFKTVIASPNSVNFHNQKVLRSTQGNLFQVNLVNEYLVTVINQLHDNNYIIIGTSLHDDSKPLSKVKFDSDDKYALIIGNEGKGISPELLDLIDLNINIEMAEDVDSINAAVAGSIIMYQINNAK"
ORIGIN
        1 ATGGAGGTGA TCTCGTCCGT TTCCAATCCC AAAATCAAGG AGATCCTTAA ATTAAAAGAC
       61 CGTAAGCACC GCAATAAACA GAAGCTGTTT ATCGTGGAGG GTTTCCATAT GATTATGGAG
      121 GCGTACAACG ACCAGATCAT TAAGACGCTT TTGGGTACCT CGAAGGCTTT AGAGATCCTT
      181 AAGGACGAGA TCCCGAACAT TGAACAGGTT ATTGAGATTT CGGAGAATGT AGCCAAAAAG
      241 ATCAGTGATA CCGTCACGTC CCAGCAAATC TTCGCAATCT GTTCGATGCC GGAAAATACT
      301 AAGATCGATT TTGAGAATAA CATCTTGCTT CTTGACCAAA TTCAAGATCC AGGAAATCTT
      361 GGTACACTTA TTCGTAGTGC GGCCAGCTTT AATTTTAAGA CGGTAATTGC CAGCCCTAAT
      421 TCGGTAAATT TCCACAATCA AAAGGTGCTG CGTAGTACTC AGGGCAACCT GTTTCAAGTG
      481 AATTTGGTAA ACGAATACCT GGTCACCGTA ATCAATCAGC TGCATGATAA TAACTACATC
      541 ATCATCGGTA CATCATTACA CGACGATTCC AAGCCTTTGT CGAAAGTCAA ATTCGACTCG
      601 GACGATAAGT ATGCCCTGAT TATCGGAAAT GAAGGCAAAG GGATTTCCCC GGAGCTTCTT
      661 GACTTGATTG ATCTGAATAT TAATATCGAG ATGGCCGAAG ATGTAGATAG CATTAATGCG
      721 GCTGTTGCGG GTTCCATCAT CATGTACCAG ATCAACAATG CTAAGTGAAG AGCTTAGAGA
      781 CCGGTACTAG TTCCTGCAGG GCTCGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA
      841 GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT
      901 TTCCTGAGAG CTTGGCAGGT GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA
      961 GAATGCCATT AATTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC
     1021 AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG
     1081 CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT
     1141 CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA
     1201 GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC
     1261 CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC
     1321 AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT
     1381 GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT
     1441 GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC
     1501 TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA
     1561 AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA
     1621 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA
     1681 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG
     1741 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG
     1801 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC
     1861 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC
     1921 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA
     1981 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC
     2041 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG
     2101 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC
     2161 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT
     2221 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG
     2281 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC
     2341 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG
     2401 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT
     2461 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG
     2521 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG
     2581 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT
     2641 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC
     2701 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC
     2761 TTGGTCAGGT GGAGTGAATT CGCGGCCGCT TCTAGAGGCT CTTCGTAATT ACGAAGAGGT
     2821 CTCTA
//