LOCUS Exported 3439 bp DNA circular SYN 24-JUL-2020 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2001) AUTHORS Keoni Gandall TITLE Direct Submission JOURNAL Exported from Poly. FEATURES Location/Qualifiers terminator 1..3439 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" terminator 38..85 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" primer_bind 1933..1949 /label="M13 for" /note="common sequencing primer, one of multiple similar variants" terminator 1950..1981 /label="tonB terminator" /note="bidirectional E. coli tonB-P14 transcription terminator" CDS 2010..3425 /label="MMSYN1_0451" /note="" /translation="MYKFKALLDGKLFDNNRILEIINPVDFSVAGQVVSLTKQDINDAFIAAKSSQKAWESTDLEKRISILDKWKQLIDQNKEELAQIIMSETAKPYKDCLTEVIRSVEYIDQTFYEVRNLKTLIIDGAKYGAKNKIGTFMRVAKGVGVAISPFNYPINLAVSKIFPCLVTGNTIVFKPATQGSLIGAKLGELAYQANLPKGIFNVVTGRGREIGDDIITNKLADFISFTGSVEVGKRLLEISSTKDVVLELGGKDPAIVLDDLDLEKYAKEIISGAFSYSGQRCTAIKRVITTDKIADQLVPLLKEKINKLTIGLPKDNCDITPLIDQKTADFVYGLIDDAKNKGAKIIIGDKQEKNLIYPTLVDHVTSDMRLAWEEPFGPVLPIIRTNSVDQMIELANKSNFGLQASVYTKNLDQALTVAQKLEVGTVNINGKSQRGPDVFPFLGVKDSGFGVQGIVDTLLFSTRYKGIVINN" primer_bind complement(90..106) /label="M13 rev" /note="common sequencing primer, one of multiple similar variants" rep_origin complement(173..761) /label="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(952..1812) /label="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*" promoter complement(1813..1917) /label="bla promoter" /note="ampicillin resistance gene promoter" ORIGIN 1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC 61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT 121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA 181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC 241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG 301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC 361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG 421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC 481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA 541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG 601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA 661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG 721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA 781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA 841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA 901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG 961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG 1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC 1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC 1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA 1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC 1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG 1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC 1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT 1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG 1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC 1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG 1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT 1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG 1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG 1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT 1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC 1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC 1981 TTGGTTCAGG TGGAGTGGGA GGGTCTCAAA TGTACAAGTT TAAAGCGCTG CTTGACGGCA 2041 AGTTGTTTGA TAATAACCGT ATCTTAGAAA TCATTAATCC CGTAGATTTT TCTGTAGCTG 2101 GACAGGTAGT CTCGCTTACC AAGCAGGACA TTAATGATGC CTTTATTGCA GCAAAGTCAA 2161 GTCAGAAAGC GTGGGAGTCT ACCGATCTTG AGAAACGTAT CTCTATTCTG GACAAATGGA 2221 AACAGTTAAT CGACCAAAAT AAGGAGGAGT TAGCACAGAT CATTATGTCC GAAACTGCCA 2281 AACCGTATAA AGACTGTCTT ACTGAGGTCA TCCGTAGTGT CGAGTACATC GACCAGACCT 2341 TTTATGAAGT CCGTAACCTG AAAACGTTAA TTATCGATGG GGCGAAATAC GGCGCGAAGA 2401 ATAAGATCGG TACATTCATG CGTGTCGCAA AGGGCGTGGG TGTGGCTATC AGTCCATTTA 2461 ATTACCCGAT TAACCTGGCT GTAAGTAAGA TCTTCCCATG CCTGGTTACG GGGAATACCA 2521 TTGTTTTCAA GCCAGCCACA CAAGGGAGCT TAATTGGTGC TAAATTAGGA GAGCTGGCAT 2581 ACCAGGCGAA TCTGCCGAAG GGGATTTTCA ATGTGGTAAC TGGGCGCGGG CGCGAGATCG 2641 GTGATGATAT TATTACTAAC AAGCTGGCTG ACTTCATCAG CTTTACCGGT AGTGTCGAAG 2701 TCGGGAAACG TTTGTTAGAA ATCTCCTCTA CGAAAGACGT GGTTTTGGAA CTTGGGGGAA 2761 AGGACCCAGC CATTGTATTG GACGATTTGG ACTTAGAGAA ATATGCCAAG GAAATCATCT 2821 CGGGAGCTTT TTCATATAGC GGTCAACGTT GCACTGCAAT TAAACGTGTT ATTACAACTG 2881 ACAAGATTGC GGACCAGTTA GTGCCCCTGC TGAAGGAGAA AATTAACAAG CTGACCATCG 2941 GGCTGCCCAA GGATAACTGT GACATTACGC CCTTGATTGA TCAAAAGACG GCAGACTTTG 3001 TATACGGATT GATCGATGAC GCAAAGAACA AGGGCGCTAA GATCATCATC GGTGACAAAC 3061 AGGAGAAAAA CCTGATTTAC CCCACCTTGG TGGATCACGT AACCTCCGAC ATGCGCTTAG 3121 CTTGGGAGGA GCCATTCGGA CCTGTGTTAC CCATCATCCG CACAAACTCT GTGGACCAGA 3181 TGATCGAACT GGCGAACAAG TCGAATTTTG GCTTACAGGC CTCAGTTTAT ACCAAGAACC 3241 TTGATCAAGC ATTGACCGTC GCCCAAAAAC TGGAGGTCGG TACAGTGAAT ATCAATGGTA 3301 AATCACAACG CGGTCCAGAC GTTTTCCCTT TCTTGGGTGT GAAAGATTCC GGGTTTGGCG 3361 TACAGGGCAT CGTAGACACT CTTTTGTTTT CTACTCGCTA CAAAGGTATT GTCATTAACA 3421 ACTGAAGAGC TTAGAGACC //