LOCUS       Exported     3989 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3989
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
                     /label="T7 terminator"
     terminator      2002..2049
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     3877..3893
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      3894..3925
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
                     /label="tonB terminator"
     CDS             1..0
                     /label="MMSYN1_0452"
                     /note="DNA topoisomerase IV, B subunit"
                     /translation="MAENKKYDESAIQVLEGLDAVRKRPGMYIGSTDNRGLHHLVWEIVDNAIDEALAGYCTQIDVILEKDNSITVIDNGRGIPTGMHKTGKSTPEVIFSVLHAGGKFDSTAYKSSGGLHGVGSSVTNALSKRFKATIYRDKKIHEIEFKNGGKLEKPLTFIANTYKTGTTINFLPDDSIFSNTKFNFSLISERLKESALLNSGLKITLSDLISNRYVEYQFQDGLVEFIKELVDDKTPVTDIITINNESKNIIAEIALQYTEDDNEIILGFANNVKTSDGGTHLVGFKSGLIRAINDYAKDQKILKDKTKLDSNDLREGLVAIVTVKIPENLIEYEGQTKSKLGTSDAKTVVEQIVYEFMSYWLIENKVLANKVIENALNAQKARIAAKQARQAIKSVKGKKNVNKLMLGKLTPAQGKKRELNELYLVEGDSAGGSAKSGRDRNFQAILPLRGKVINSEKAKLVDLLKNEEIQSIINAIGAGVGKDFDISDINYGKIIIMTDADTDGAHIQTLLLTFFYRHMKDLITHKKVYIALPPLYKITFNDKSFIYLWDEEELNEFNKTNTKKYEIQRYKGLGEMNADQLWQTTMDPKNRKIIQVSISDGLLAERMFKTLMGDDVEKRKLWIQENVKFTLEDDQIQIIEMEK"
     primer_bind     complement(2054..2070)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(2137..2725)
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
                     /label="ori"
     CDS             complement(2896..3756)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(3757..3861)
                     /note="ampicillin resistance gene promoter"
                     /label="bla promoter"
     CDS             complement(1..0)
                     /label="MMSYN1_0452"
                     /note="DNA topoisomerase IV, B subunit"
                     /translation="MAENKKYDESAIQVLEGLDAVRKRPGMYIGSTDNRGLHHLVWEIVDNAIDEALAGYCTQIDVILEKDNSITVIDNGRGIPTGMHKTGKSTPEVIFSVLHAGGKFDSTAYKSSGGLHGVGSSVTNALSKRFKATIYRDKKIHEIEFKNGGKLEKPLTFIANTYKTGTTINFLPDDSIFSNTKFNFSLISERLKESALLNSGLKITLSDLISNRYVEYQFQDGLVEFIKELVDDKTPVTDIITINNESKNIIAEIALQYTEDDNEIILGFANNVKTSDGGTHLVGFKSGLIRAINDYAKDQKILKDKTKLDSNDLREGLVAIVTVKIPENLIEYEGQTKSKLGTSDAKTVVEQIVYEFMSYWLIENKVLANKVIENALNAQKARIAAKQARQAIKSVKGKKNVNKLMLGKLTPAQGKKRELNELYLVEGDSAGGSAKSGRDRNFQAILPLRGKVINSEKAKLVDLLKNEEIQSIINAIGAGVGKDFDISDINYGKIIIMTDADTDGAHIQTLLLTFFYRHMKDLITHKKVYIALPPLYKITFNDKSFIYLWDEEELNEFNKTNTKKYEIQRYKGLGEMNADQLWQTTMDPKNRKIIQVSISDGLLAERMFKTLMGDDVEKRKLWIQENVKFTLEDDQIQIIEMEK"
ORIGIN
        1 ATGGCCGAAA ACAAGAAATA TGACGAGTCT GCGATCCAGG TATTAGAAGG TCTGGACGCC
       61 GTCCGTAAAC GCCCAGGAAT GTACATTGGT TCTACTGACA ACCGTGGCCT GCATCACTTG
      121 GTGTGGGAGA TTGTTGACAA TGCTATCGAC GAAGCTCTGG CGGGTTATTG CACCCAAATT
      181 GACGTGATTT TAGAAAAGGA TAACTCGATT ACAGTTATTG ATAACGGCCG TGGCATTCCC
      241 ACAGGTATGC ATAAGACAGG AAAATCTACG CCGGAAGTTA TCTTCTCCGT TTTGCACGCT
      301 GGGGGAAAGT TCGATAGCAC AGCTTATAAA TCTAGCGGCG GGTTACATGG TGTAGGTTCA
      361 TCCGTCACGA ATGCGCTTTC CAAACGTTTT AAGGCTACCA TTTATCGCGA CAAGAAAATC
      421 CACGAGATTG AGTTCAAGAA TGGTGGTAAA TTGGAAAAAC CACTTACCTT TATCGCAAAC
      481 ACCTACAAAA CAGGTACAAC CATCAACTTT TTGCCGGATG ATTCAATCTT TTCTAACACG
      541 AAGTTTAATT TTAGTTTAAT TAGTGAACGT CTTAAGGAGT CGGCTTTATT GAACTCGGGG
      601 CTTAAAATCA CCTTAAGTGA CCTTATTAGT AATCGTTACG TGGAATACCA ATTCCAGGAT
      661 GGTTTAGTTG AATTTATCAA AGAATTGGTG GACGACAAAA CGCCTGTAAC AGACATCATC
      721 ACGATCAATA ATGAGAGCAA GAACATTATT GCAGAGATTG CGTTACAATA CACGGAGGAC
      781 GATAATGAAA TCATCCTGGG CTTCGCTAAC AATGTAAAGA CGAGTGACGG GGGAACCCAT
      841 CTGGTAGGCT TTAAATCCGG GTTAATCCGT GCCATTAATG ATTACGCAAA AGACCAAAAG
      901 ATCTTAAAGG ATAAAACTAA GTTGGACAGT AATGATCTGC GTGAAGGTTT GGTAGCCATT
      961 GTTACAGTCA AGATCCCGGA AAACCTTATT GAGTATGAGG GTCAGACTAA AAGCAAGCTT
     1021 GGCACCTCTG ATGCTAAGAC CGTGGTAGAA CAAATTGTGT ACGAGTTCAT GTCTTATTGG
     1081 TTAATTGAGA ACAAGGTCCT GGCTAACAAG GTGATCGAGA ATGCTCTGAA CGCCCAAAAA
     1141 GCTCGCATTG CAGCGAAACA AGCGCGTCAG GCTATTAAGT CTGTTAAGGG CAAGAAAAAT
     1201 GTCAACAAGC TGATGTTAGG AAAGCTTACG CCCGCCCAGG GCAAAAAACG TGAATTAAAT
     1261 GAGCTTTATT TGGTGGAGGG GGATTCAGCG GGCGGGTCCG CGAAGAGTGG CCGCGACCGT
     1321 AATTTTCAGG CCATCTTACC TTTGCGCGGC AAGGTAATCA ATTCCGAGAA AGCGAAGTTA
     1381 GTCGATCTGT TGAAAAATGA AGAGATCCAA AGCATCATCA ATGCTATTGG GGCAGGCGTC
     1441 GGTAAAGACT TTGATATTTC CGATATTAAT TATGGCAAAA TTATTATTAT GACTGATGCA
     1501 GACACGGACG GTGCTCATAT CCAGACCTTG CTTTTGACCT TCTTTTATCG CCACATGAAG
     1561 GATTTAATCA CTCATAAAAA AGTTTACATT GCGTTACCTC CTTTGTACAA AATTACGTTT
     1621 AACGACAAAA GTTTTATCTA CCTGTGGGAC GAGGAAGAAT TAAATGAGTT CAACAAGACA
     1681 AACACTAAGA AGTATGAGAT TCAACGTTAT AAGGGCTTAG GCGAAATGAA CGCGGACCAA
     1741 CTGTGGCAGA CTACGATGGA CCCCAAGAAT CGCAAAATTA TCCAGGTATC CATCTCGGAC
     1801 GGTCTGTTGG CGGAACGTAT GTTTAAGACC TTGATGGGAG ATGACGTTGA AAAACGCAAG
     1861 TTGTGGATTC AAGAAAATGT CAAGTTCACT TTGGAGGACG ACCAGATCCA GATTATTGAG
     1921 ATGGAGAAGT GAAGAGCTTA GAGACCGGTA CTAGTTCCTG CAGGGCTCGA GGTGTCAATC
     1981 GTCGGAGCCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG
     2041 GGTTTTTTGC ATGGTCATAG CTGTTTCCTG AGAGCTTGGC AGGTGATGAC ACACATTAAC
     2101 AAATTTCGTG AGGAGTCTCC AGAAGAATGC CATTAATTTC CATAGGCTCC GCCCCCCTGA
     2161 CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG
     2221 ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT
     2281 TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG
     2341 CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC
     2401 CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT
     2461 AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA
     2521 TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC
     2581 AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC
     2641 TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT
     2701 TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC
     2761 TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT
     2821 CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA
     2881 AACTTGGTCT GACAGTTACC AATGCTTAAT CAGTGAGGCA CCTATCTCAG CGATCTGTCT
     2941 ATTTCGTTCA TCCATAGTTG CCTGACTCCC CGTCGTGTAG ATAACTACGA TACGGGAGGG
     3001 CTTACCATCT GGCCCCAGTG CTGCAATGAT ACCGCGAGAA CCACGCTCAC CGGCTCCAGA
     3061 TTTATCAGCA ATAAACCAGC CAGCCGGAAG GGCCGAGCGC AGAAGTGGTC CTGCAACTTT
     3121 ATCCGCCTCC ATCCAGTCTA TTAATTGTTG CCGGGAAGCT AGAGTAAGTA GTTCGCCAGT
     3181 TAATAGTTTG CGCAACGTTG TTGCCATTGC TACAGGCATC GTGGTGTCAC GCTCGTCGTT
     3241 TGGTATGGCT TCATTCAGCT CCGGTTCCCA ACGATCAAGG CGAGTTACAT GATCCCCCAT
     3301 GTTGTGCAAA AAAGCGGTTA GCTCCTTCGG TCCTCCGATC GTTGTCAGAA GTAAGTTGGC
     3361 CGCAGTGTTA TCACTCATGG TTATGGCAGC ACTGCATAAT TCTCTTACTG TCATGCCATC
     3421 CGTAAGATGC TTTTCTGTGA CTGGTGAGTA CTCAACCAAG TCATTCTGAG AATAGTGTAT
     3481 GCGGCGACCG AGTTGCTCTT GCCCGGCGTC AATACGGGAT AATACCGCGC CACATAGCAG
     3541 AACTTTAAAA GTGCTCATCA TTGGAAAACG TTCTTCGGGG CGAAAACTCT CAAGGATCTT
     3601 ACCGCTGTTG AGATCCAGTT CGATGTAACC CACTCGTGCA CCCAACTGAT CTTCAGCATC
     3661 TTTTACTTTC ACCAGCGTTT CTGGGTGAGC AAAAACAGGA AGGCAAAATG CCGCAAAAAA
     3721 GGGAATAAGG GCGACACGGA AATGTTGAAT ACTCATACTC TTCCTTTTTC AATATTATTG
     3781 AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA
     3841 TAAACAAATA GGGGTTCCGC GCACCTGCAC CAGTCAGTAA AACGACGGCC AGTAGTCAAA
     3901 AGCCTCCGAC CGGAGGCTTT TGACTTGGTC AGGTGGAGTG AATTCGCGGC CGCTTCTAGA
     3961 GGCTCTTCGT AATTACGAAG AGGTCTCTA
//