LOCUS       Exported     3014 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3014
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1027..1074
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     2902..2918
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      2919..2950
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             1..0
                     /label="MMSYN1_0475"
                     /note="L-LDH-NAD: L-lactate dehydrogenase"
                     /translation="MKKTANKVVLIGAGAVGTSFLYAAINQGIAEHYVLIDAFPQPAEGNALDLSDTLAVIPHSFSSIKAGTYEDCKDADVVVITAGRPQKPGETRLEMVAGNAEIMKNIATEVKKSGFDGITVIASNPVDVITHVYQKVTGFDPHKVIGSGTTLDSARLRRLVGQKLNVKPESVQAYVAGEHGDSSVAIWSQANIMGRPILDYVKCGCLTLEDLDQIQKDTVDMAYKIINLKRATFYGIGACLTKIVNAVLRDEKSTLMVGAQLNGEYKNKGLYTGVPAIIGSNGWERIIEWDLTKEEQEKFDKSCETLHKTIDSVKHLFE"
     primer_bind     complement(1079..1095)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1162..1750)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(1921..2781)
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
                     /label="bla"
     promoter        complement(2782..2886)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
     CDS             complement(1..0)
                     /note="L-LDH-NAD: L-lactate dehydrogenase"
                     /translation="MKKTANKVVLIGAGAVGTSFLYAAINQGIAEHYVLIDAFPQPAEGNALDLSDTLAVIPHSFSSIKAGTYEDCKDADVVVITAGRPQKPGETRLEMVAGNAEIMKNIATEVKKSGFDGITVIASNPVDVITHVYQKVTGFDPHKVIGSGTTLDSARLRRLVGQKLNVKPESVQAYVAGEHGDSSVAIWSQANIMGRPILDYVKCGCLTLEDLDQIQKDTVDMAYKIINLKRATFYGIGACLTKIVNAVLRDEKSTLMVGAQLNGEYKNKGLYTGVPAIIGSNGWERIIEWDLTKEEQEKFDKSCETLHKTIDSVKHLFE"
                     /label="MMSYN1_0475"
ORIGIN
        1 ATGAAGAAAA CGGCAAACAA GGTAGTACTT ATTGGTGCCG GGGCCGTCGG TACGAGCTTT
       61 CTTTACGCAG CTATTAACCA GGGTATTGCC GAGCACTACG TATTAATCGA TGCGTTCCCT
      121 CAGCCTGCTG AGGGCAATGC CTTAGACCTG TCGGATACAC TGGCAGTAAT TCCACACTCG
      181 TTTAGTAGTA TCAAAGCCGG TACTTATGAA GATTGCAAAG ACGCCGATGT AGTGGTAATC
      241 ACAGCGGGTC GTCCGCAGAA GCCCGGCGAA ACTCGTTTAG AAATGGTCGC TGGTAACGCA
      301 GAAATCATGA AAAATATTGC TACGGAGGTT AAAAAATCTG GCTTTGATGG CATCACTGTA
      361 ATCGCATCAA ATCCCGTGGA CGTAATTACA CACGTCTACC AGAAAGTGAC CGGCTTTGAC
      421 CCACATAAAG TAATTGGATC GGGCACCACG TTAGATTCTG CACGCTTGCG CCGCTTGGTC
      481 GGCCAAAAAC TTAATGTCAA GCCAGAGTCG GTACAGGCAT ACGTGGCGGG TGAACACGGC
      541 GATTCTTCCG TAGCCATTTG GTCCCAGGCA AATATCATGG GTCGTCCCAT TCTGGATTAC
      601 GTGAAGTGCG GGTGCTTAAC GCTGGAGGAC TTAGACCAGA TCCAAAAAGA CACGGTCGAT
      661 ATGGCATACA AAATTATTAA TCTGAAGCGC GCTACGTTTT ACGGCATTGG AGCTTGCCTT
      721 ACAAAGATTG TGAACGCTGT TCTTCGTGAT GAAAAATCAA CATTGATGGT GGGCGCACAG
      781 CTTAACGGTG AATATAAGAA TAAGGGATTA TACACAGGGG TCCCTGCCAT TATTGGGTCG
      841 AATGGATGGG AGCGCATCAT CGAATGGGAT CTGACGAAAG AAGAACAAGA AAAATTCGAC
      901 AAATCCTGCG AAACGCTGCA TAAGACTATT GATAGTGTTA AACACCTGTT CGAGTGAAGA
      961 GCTTAGAGAC CGGTACTAGT TCCTGCAGGG CTCGAGGTGT CAATCGTCGG AGCCGCTGAG
     1021 CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCATGGT
     1081 CATAGCTGTT TCCTGAGAGC TTGGCAGGTG ATGACACACA TTAACAAATT TCGTGAGGAG
     1141 TCTCCAGAAG AATGCCATTA ATTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA
     1201 TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC
     1261 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC
     1321 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG
     1381 TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA
     1441 CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC
     1501 GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC
     1561 AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG
     1621 CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA
     1681 AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA
     1741 AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA
     1801 CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT
     1861 AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAAACTT GGTCTGACAG
     1921 TTACCAATGC TTAATCAGTG AGGCACCTAT CTCAGCGATC TGTCTATTTC GTTCATCCAT
     1981 AGTTGCCTGA CTCCCCGTCG TGTAGATAAC TACGATACGG GAGGGCTTAC CATCTGGCCC
     2041 CAGTGCTGCA ATGATACCGC GAGAACCACG CTCACCGGCT CCAGATTTAT CAGCAATAAA
     2101 CCAGCCAGCC GGAAGGGCCG AGCGCAGAAG TGGTCCTGCA ACTTTATCCG CCTCCATCCA
     2161 GTCTATTAAT TGTTGCCGGG AAGCTAGAGT AAGTAGTTCG CCAGTTAATA GTTTGCGCAA
     2221 CGTTGTTGCC ATTGCTACAG GCATCGTGGT GTCACGCTCG TCGTTTGGTA TGGCTTCATT
     2281 CAGCTCCGGT TCCCAACGAT CAAGGCGAGT TACATGATCC CCCATGTTGT GCAAAAAAGC
     2341 GGTTAGCTCC TTCGGTCCTC CGATCGTTGT CAGAAGTAAG TTGGCCGCAG TGTTATCACT
     2401 CATGGTTATG GCAGCACTGC ATAATTCTCT TACTGTCATG CCATCCGTAA GATGCTTTTC
     2461 TGTGACTGGT GAGTACTCAA CCAAGTCATT CTGAGAATAG TGTATGCGGC GACCGAGTTG
     2521 CTCTTGCCCG GCGTCAATAC GGGATAATAC CGCGCCACAT AGCAGAACTT TAAAAGTGCT
     2581 CATCATTGGA AAACGTTCTT CGGGGCGAAA ACTCTCAAGG ATCTTACCGC TGTTGAGATC
     2641 CAGTTCGATG TAACCCACTC GTGCACCCAA CTGATCTTCA GCATCTTTTA CTTTCACCAG
     2701 CGTTTCTGGG TGAGCAAAAA CAGGAAGGCA AAATGCCGCA AAAAAGGGAA TAAGGGCGAC
     2761 ACGGAAATGT TGAATACTCA TACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG
     2821 TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT
     2881 TCCGCGCACC TGCACCAGTC AGTAAAACGA CGGCCAGTAG TCAAAAGCCT CCGACCGGAG
     2941 GCTTTTGACT TGGTCAGGTG GAGTGAATTC GCGGCCGCTT CTAGAGGCTC TTCGTAATTA
     3001 CGAAGAGGTC TCTA
//