LOCUS       Exported     3670 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3670
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      38..85
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
                     /label="T7 terminator"
     primer_bind     1933..1949
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      1950..1981
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             2010..3656
                     /note=""
                     /translation="MKKVFSYFLIILIFFTSLFFINNKNQNQVNLTYNTQFNDNDNNETNKNSIKEFLWGGKALRYFLYKNSTAQTNKSFNQFTDNLLANFERVFQKRTKRNFYKQQYITELQSEEFKHAILSSILVTSAYGSTSPEEFFAESFSRYVSANEKQKNLTWYLLEHFFTKTFYKLKQQNIGILPSNDKEINWKKIKNVIDSENDVKYKYELEPENHTLNSQYDRLNYFDLGYHTNQYGYNNGLYIFETINYIYKNTFAPQISNLDFLNLDRSVLNGDRFAHYYRDNYDIFSDYMKLNLYKPKNIITTNSNDQFFKDFDQLDAYWKEKSKFNFGKSSAIQIKQNLENIWNAIPKPKTLNKDYFDLDKLKTNTVHLFNTLQKVTHNNLDNIFINLILTNDDRFKVNNNLLDPKIKGITSTSFSKNTLSSSYSYVLIKADSFNKAENQEQYDRSWFASNNQFQTLNHEFGHVLDSFLALNSYQTQLNKNTFSSLSFWADHQQANLYHGNIVISKNRNWSLYSIFIIGVIGINLVLLILYIGYDKIFKPKNKKTIVIK"
                     /label="MMSYN1_0479"
     primer_bind     complement(90..106)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(173..761)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(952..1812)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(1813..1917)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC
       61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT
      121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA
      181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC
      241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG
      301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC
      361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG
      421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC
      481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA
      541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG
      601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA
      661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG
      721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA
      781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA
      841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA
      901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG
      961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG
     1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC
     1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC
     1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA
     1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC
     1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG
     1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC
     1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT
     1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG
     1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC
     1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG
     1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT
     1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG
     1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG
     1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT
     1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC
     1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC
     1981 TTGGTTCAGG TGGAGTGGGA GGGTCTCAAA TGAAGAAAGT TTTCTCGTAT TTTTTGATCA
     2041 TCCTGATTTT CTTCACCTCG CTGTTCTTTA TCAACAACAA GAATCAGAAC CAGGTTAACC
     2101 TTACGTATAA CACACAATTT AATGACAATG ACAACAATGA GACTAATAAG AATTCCATCA
     2161 AGGAGTTTTT ATGGGGTGGT AAGGCTCTGC GTTACTTTTT ATACAAAAAT AGTACAGCCC
     2221 AAACGAATAA ATCCTTCAAT CAATTCACCG ACAACTTATT GGCCAACTTC GAGCGTGTTT
     2281 TTCAAAAACG CACAAAGCGC AACTTCTATA AGCAACAGTA CATCACTGAA CTGCAAAGCG
     2341 AGGAGTTCAA GCATGCCATT TTATCCTCTA TTCTTGTAAC GTCCGCTTAT GGCAGTACAA
     2401 GTCCTGAAGA ATTTTTTGCC GAGTCTTTCA GCCGTTATGT AAGTGCTAAT GAGAAACAGA
     2461 AGAATTTAAC ATGGTACCTT TTGGAGCATT TCTTCACCAA GACGTTCTAT AAACTGAAAC
     2521 AGCAAAATAT TGGAATTCTT CCGTCCAACG ATAAAGAAAT TAACTGGAAG AAAATTAAGA
     2581 ACGTAATCGA CAGCGAAAAC GACGTTAAAT ATAAATATGA ATTAGAACCC GAAAACCACA
     2641 CACTTAATAG TCAATATGAC CGCTTAAACT ACTTTGATTT GGGGTATCAC ACAAACCAAT
     2701 ACGGTTACAA TAATGGGCTG TACATCTTCG AAACTATTAA CTACATTTAT AAAAATACCT
     2761 TTGCCCCGCA AATTTCGAAC CTTGATTTTT TGAACCTGGA TCGTTCTGTG CTTAATGGGG
     2821 ATCGTTTTGC ACATTATTAT CGTGACAATT ATGATATTTT TAGTGACTAT ATGAAATTAA
     2881 ATCTGTACAA GCCGAAAAAT ATCATCACAA CGAACAGTAA TGACCAGTTT TTCAAGGATT
     2941 TTGATCAGTT GGACGCATAT TGGAAGGAAA AAAGCAAGTT TAACTTCGGC AAATCGTCGG
     3001 CAATTCAGAT CAAACAGAAT CTGGAAAACA TCTGGAACGC TATTCCAAAA CCGAAAACTC
     3061 TGAATAAGGA TTATTTTGAT CTGGACAAAC TTAAGACCAA CACAGTACAC CTGTTTAATA
     3121 CCCTTCAAAA AGTCACACAC AATAACTTGG ACAACATTTT CATTAATTTG ATTTTAACAA
     3181 ATGATGATCG CTTTAAGGTC AATAACAACT TGCTTGACCC TAAAATTAAG GGTATTACTT
     3241 CGACTTCGTT TTCGAAAAAC ACGCTGTCCT CGTCTTACAG TTACGTATTA ATCAAAGCTG
     3301 ATTCCTTCAA TAAAGCTGAA AATCAGGAAC AGTATGACCG TTCTTGGTTT GCCTCTAACA
     3361 ACCAGTTCCA GACCCTTAAC CATGAGTTCG GACACGTTTT GGATTCGTTT TTGGCGTTAA
     3421 ATTCTTATCA GACTCAGCTG AACAAGAATA CATTTAGCTC TTTATCGTTT TGGGCCGATC
     3481 ACCAGCAGGC AAACTTATAC CACGGCAACA TTGTTATTTC GAAGAATCGT AATTGGAGCT
     3541 TGTACTCAAT CTTCATTATC GGTGTGATTG GCATCAACCT TGTCTTATTG ATCTTGTATA
     3601 TTGGCTACGA TAAGATTTTC AAGCCGAAGA ACAAAAAGAC GATCGTAATC AAGTGAAGAG
     3661 CTTAGAGACC
//