LOCUS Exported 2390 bp DNA circular SYN 24-JUL-2020 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2001) AUTHORS Keoni Gandall TITLE Direct Submission JOURNAL Exported from Poly. FEATURES Location/Qualifiers terminator 1..2390 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" terminator 403..450 /note="transcription terminator for bacteriophage T7 RNA polymerase" /label="T7 terminator" primer_bind 2278..2294 /note="common sequencing primer, one of multiple similar variants" /label="M13 for" terminator 2295..2326 /label="tonB terminator" /note="bidirectional E. coli tonB-P14 transcription terminator" CDS 1..0 /label="MMSYN1_0513" /note="Transfers the 4'-phosphopantetheine moiety from coenzyme A to a Ser of acyl-carrier-protein." /translation="MINNVGIDIVENKRIKLKKEFIIKVLSTNEIQTFNTKTKKQKKEFLAGRWAVKEAIIKTLDQAISMNKIDIEYVNQKPVIQNKELQNILISISHEKKYAIGIALKQSDNK" primer_bind complement(455..471) /label="M13 rev" /note="common sequencing primer, one of multiple similar variants" rep_origin complement(538..1126) /label="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(1297..2157) /label="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*" promoter complement(2158..2262) /label="bla promoter" /note="ampicillin resistance gene promoter" CDS complement(1..0) /translation="MINNVGIDIVENKRIKLKKEFIIKVLSTNEIQTFNTKTKKQKKEFLAGRWAVKEAIIKTLDQAISMNKIDIEYVNQKPVIQNKELQNILISISHEKKYAIGIALKQSDNK" /label="MMSYN1_0513" /note="Transfers the 4'-phosphopantetheine moiety from coenzyme A to a Ser of acyl-carrier-protein." ORIGIN 1 ATGATTAACA ATGTGGGTAT CGACATCGTG GAAAATAAGC GTATCAAGCT GAAAAAAGAG 61 TTTATTATCA AAGTTCTTAG TACGAACGAG ATCCAGACAT TCAACACAAA AACGAAAAAG 121 CAGAAGAAAG AATTTTTGGC CGGACGCTGG GCTGTGAAAG AAGCTATCAT TAAGACCTTA 181 GACCAGGCAA TCTCAATGAA CAAAATTGAC ATCGAGTACG TGAATCAAAA GCCAGTGATC 241 CAGAACAAAG AACTTCAGAA TATTTTGATT TCTATTTCGC ACGAGAAAAA ATATGCGATT 301 GGAATTGCGT TAAAACAGTC AGATAATAAG TGAAGAGCTT AGAGACCGGT ACTAGTTCCT 361 GCAGGGCTCG AGGTGTCAAT CGTCGGAGCC GCTGAGCAAT AACTAGCATA ACCCCTTGGG 421 GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CATGGTCATA GCTGTTTCCT GAGAGCTTGG 481 CAGGTGATGA CACACATTAA CAAATTTCGT GAGGAGTCTC CAGAAGAATG CCATTAATTT 541 CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG 601 AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC 661 TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT 721 GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA 781 GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA 841 TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA 901 CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA 961 CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT 1021 CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT 1081 TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT 1141 CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT 1201 GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC 1261 AATCTAAAGT ATATATGAGT AAACTTGGTC TGACAGTTAC CAATGCTTAA TCAGTGAGGC 1321 ACCTATCTCA GCGATCTGTC TATTTCGTTC ATCCATAGTT GCCTGACTCC CCGTCGTGTA 1381 GATAACTACG ATACGGGAGG GCTTACCATC TGGCCCCAGT GCTGCAATGA TACCGCGAGA 1441 ACCACGCTCA CCGGCTCCAG ATTTATCAGC AATAAACCAG CCAGCCGGAA GGGCCGAGCG 1501 CAGAAGTGGT CCTGCAACTT TATCCGCCTC CATCCAGTCT ATTAATTGTT GCCGGGAAGC 1561 TAGAGTAAGT AGTTCGCCAG TTAATAGTTT GCGCAACGTT GTTGCCATTG CTACAGGCAT 1621 CGTGGTGTCA CGCTCGTCGT TTGGTATGGC TTCATTCAGC TCCGGTTCCC AACGATCAAG 1681 GCGAGTTACA TGATCCCCCA TGTTGTGCAA AAAAGCGGTT AGCTCCTTCG GTCCTCCGAT 1741 CGTTGTCAGA AGTAAGTTGG CCGCAGTGTT ATCACTCATG GTTATGGCAG CACTGCATAA 1801 TTCTCTTACT GTCATGCCAT CCGTAAGATG CTTTTCTGTG ACTGGTGAGT ACTCAACCAA 1861 GTCATTCTGA GAATAGTGTA TGCGGCGACC GAGTTGCTCT TGCCCGGCGT CAATACGGGA 1921 TAATACCGCG CCACATAGCA GAACTTTAAA AGTGCTCATC ATTGGAAAAC GTTCTTCGGG 1981 GCGAAAACTC TCAAGGATCT TACCGCTGTT GAGATCCAGT TCGATGTAAC CCACTCGTGC 2041 ACCCAACTGA TCTTCAGCAT CTTTTACTTT CACCAGCGTT TCTGGGTGAG CAAAAACAGG 2101 AAGGCAAAAT GCCGCAAAAA AGGGAATAAG GGCGACACGG AAATGTTGAA TACTCATACT 2161 CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT 2221 ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACCTGCA CCAGTCAGTA 2281 AAACGACGGC CAGTAGTCAA AAGCCTCCGA CCGGAGGCTT TTGACTTGGT CAGGTGGAGT 2341 GAATTCGCGG CCGCTTCTAG AGGCTCTTCG TAATTACGAA GAGGTCTCTA //