LOCUS       Exported     2666 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..2666
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      679..726
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     2554..2570
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      2571..2602
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             1..0
                     /note="lspA: Lipoprotein signal peptidase"
                     /translation="MWIKDKLVETKVFLKNHNYLWKFKLIVCLPIFISLISFDWISKAIVVSHMKLGETKTFINGFLNFQYVINLGMAYGKLHDKAYLVIIFATIFSLFLTTIFIFLNNKKWLIVLVIILAGSWGNLLARLWAPGNENNVYYGVVDFLTWDFSLFNSRDYVFNLADLYVNIAIGLTILFTIIELVLYIKSKIKTKKEKIDNEQNNS"
                     /label="MMSYN1_0518"
     primer_bind     complement(731..747)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(814..1402)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(1573..2433)
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
                     /label="bla"
     promoter        complement(2434..2538)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
     CDS             complement(1..0)
                     /note="lspA: Lipoprotein signal peptidase"
                     /translation="MWIKDKLVETKVFLKNHNYLWKFKLIVCLPIFISLISFDWISKAIVVSHMKLGETKTFINGFLNFQYVINLGMAYGKLHDKAYLVIIFATIFSLFLTTIFIFLNNKKWLIVLVIILAGSWGNLLARLWAPGNENNVYYGVVDFLTWDFSLFNSRDYVFNLADLYVNIAIGLTILFTIIELVLYIKSKIKTKKEKIDNEQNNS"
                     /label="MMSYN1_0518"
ORIGIN
        1 ATGTGGATCA AAGATAAGTT GGTCGAAACG AAAGTGTTTC TGAAAAACCA CAATTACCTG
       61 TGGAAATTCA AGCTTATCGT TTGCCTTCCG ATTTTTATCT CATTAATCTC GTTTGATTGG
      121 ATCAGCAAAG CAATCGTTGT CTCTCACATG AAGTTAGGTG AAACGAAAAC CTTCATTAAC
      181 GGCTTCCTTA ATTTTCAATA TGTCATCAAT CTTGGGATGG CTTATGGAAA ATTACATGAT
      241 AAAGCTTATC TTGTTATTAT CTTCGCTACG ATTTTCTCCT TGTTCTTAAC GACGATCTTC
      301 ATCTTCCTTA ACAACAAGAA GTGGTTAATC GTATTGGTTA TCATTTTAGC CGGGAGTTGG
      361 GGGAACCTTT TGGCGCGCCT GTGGGCGCCC GGTAATGAGA ACAATGTCTA CTACGGAGTT
      421 GTTGATTTTC TGACGTGGGA TTTCAGTCTG TTCAACTCCC GTGACTATGT ATTTAACCTG
      481 GCTGACTTAT ATGTCAATAT CGCGATTGGT TTAACTATTC TGTTCACTAT TATCGAACTG
      541 GTACTGTATA TTAAATCCAA GATTAAAACT AAGAAGGAAA AGATCGACAA CGAGCAGAAT
      601 AATTCCTGAA GAGCTTAGAG ACCGGTACTA GTTCCTGCAG GGCTCGAGGT GTCAATCGTC
      661 GGAGCCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT
      721 TTTTTGCATG GTCATAGCTG TTTCCTGAGA GCTTGGCAGG TGATGACACA CATTAACAAA
      781 TTTCGTGAGG AGTCTCCAGA AGAATGCCAT TAATTTCCAT AGGCTCCGCC CCCCTGACGA
      841 GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA
      901 CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC
      961 CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG
     1021 TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC
     1081 CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG
     1141 ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT
     1201 AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT
     1261 ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG
     1321 ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC
     1381 GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA
     1441 GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC
     1501 CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAAAC
     1561 TTGGTCTGAC AGTTACCAAT GCTTAATCAG TGAGGCACCT ATCTCAGCGA TCTGTCTATT
     1621 TCGTTCATCC ATAGTTGCCT GACTCCCCGT CGTGTAGATA ACTACGATAC GGGAGGGCTT
     1681 ACCATCTGGC CCCAGTGCTG CAATGATACC GCGAGAACCA CGCTCACCGG CTCCAGATTT
     1741 ATCAGCAATA AACCAGCCAG CCGGAAGGGC CGAGCGCAGA AGTGGTCCTG CAACTTTATC
     1801 CGCCTCCATC CAGTCTATTA ATTGTTGCCG GGAAGCTAGA GTAAGTAGTT CGCCAGTTAA
     1861 TAGTTTGCGC AACGTTGTTG CCATTGCTAC AGGCATCGTG GTGTCACGCT CGTCGTTTGG
     1921 TATGGCTTCA TTCAGCTCCG GTTCCCAACG ATCAAGGCGA GTTACATGAT CCCCCATGTT
     1981 GTGCAAAAAA GCGGTTAGCT CCTTCGGTCC TCCGATCGTT GTCAGAAGTA AGTTGGCCGC
     2041 AGTGTTATCA CTCATGGTTA TGGCAGCACT GCATAATTCT CTTACTGTCA TGCCATCCGT
     2101 AAGATGCTTT TCTGTGACTG GTGAGTACTC AACCAAGTCA TTCTGAGAAT AGTGTATGCG
     2161 GCGACCGAGT TGCTCTTGCC CGGCGTCAAT ACGGGATAAT ACCGCGCCAC ATAGCAGAAC
     2221 TTTAAAAGTG CTCATCATTG GAAAACGTTC TTCGGGGCGA AAACTCTCAA GGATCTTACC
     2281 GCTGTTGAGA TCCAGTTCGA TGTAACCCAC TCGTGCACCC AACTGATCTT CAGCATCTTT
     2341 TACTTTCACC AGCGTTTCTG GGTGAGCAAA AACAGGAAGG CAAAATGCCG CAAAAAAGGG
     2401 AATAAGGGCG ACACGGAAAT GTTGAATACT CATACTCTTC CTTTTTCAAT ATTATTGAAG
     2461 CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA
     2521 ACAAATAGGG GTTCCGCGCA CCTGCACCAG TCAGTAAAAC GACGGCCAGT AGTCAAAAGC
     2581 CTCCGACCGG AGGCTTTTGA CTTGGTCAGG TGGAGTGAAT TCGCGGCCGC TTCTAGAGGC
     2641 TCTTCGTAAT TACGAAGAGG TCTCTA
//