LOCUS       BBF10K_000246           4740 bp    DNA     circular UNK 12-JAN-2020
DEFINITION  Yeast cds LIS (S-linalool synthase) in pOpen-v3.
ACCESSION   BBF10K_000246
VERSION     BBF10K_000246.1
KEYWORDS    BBFD_0017; Open Yeast Collection Part; OpenMTA; FreeGenes; Golden
            Gate Assembly.
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
            .
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
REFERENCE   2  (bases 2002 to 4740)
  AUTHORS   Scott Pownall, Open Science Network
  TITLE     Direct Submission
  JOURNAL   https://biobricks.org/freegenes/
FEATURES             Location/Qualifiers
     source          1..2001
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
                     /original_record="pOpen_v3.1"
     primer_bind     complement(24..42)
                     /label="T7 Reverse"
                     /note="/ApEinfo_fwdcolor=#b7e6d7 /ApEinfo_revcolor=#b7e6d7"
                     /original_record="pOpen_v3.1"
     terminator      38..85
                     /label="T7 terminator"
                     /note="/ApEinfo_fwdcolor=#d6b295 /ApEinfo_revcolor=#d6b295"
                     /original_record="pOpen_v3.1"
     primer_bind     complement(86..106)
                     /label="M13 rev"
                     /note="/ApEinfo_fwdcolor=#ff9ccd /ApEinfo_revcolor=#ff9ccd"
                     /original_record="pOpen_v3.1"
     misc_feature    107..121
                     /label="AarI"
                     /note="/ApEinfo_fwdcolor=#b1ff67 /ApEinfo_revcolor=#b1ff67"
                     /original_record="pOpen_v3.1"
     misc_feature    122..172
                     /label="spacer"
                     /note="/ApEinfo_fwdcolor=#b1ff67 /ApEinfo_revcolor=#b1ff67"
                     /original_record="pOpen_v3.1"
     rep_origin      complement(173..761)
                     /direction=LEFT
                     /label="ori"
                     /note="/ApEinfo_fwdcolor=#84b0dc /ApEinfo_revcolor=#84b0dc"
                     /original_record="pOpen_v3.1"
     CDS             complement(952..1812)
                     /label="AmpR"
                     /note="/ApEinfo_fwdcolor=#ff9ccd /ApEinfo_revcolor=#ff9ccd"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW*"
                     /original_record="pOpen_v3.1"
     misc_feature    1918..1932
                     /label="AarI"
                     /note="/ApEinfo_fwdcolor=#d6b295 /ApEinfo_revcolor=#d6b295"
                     /original_record="pOpen_v3.1"
     primer_bind     1933..1949
                     /label="M13 fwd"
                     /note="/ApEinfo_fwdcolor=#84b0dc /ApEinfo_revcolor=#84b0dc"
                     /original_record="pOpen_v3.1"
     terminator      1950..1981
                     /label="tonB terminator"
                     /note="/ApEinfo_fwdcolor=#b4abac /ApEinfo_revcolor=#b4abac"
                     /original_record="pOpen_v3.1"
     CDS             2030..4642
                     /codon_start=1
                     /gene="S-linalool synthase"
                     /product="S-linalool synthase"
                     /label="S-linalool synthase"
                     /translation="MQLITNFSSSSSELQFLVDKVKRESLSSSSSNTQNLFLSTSPYDT
                     AWLALIPHPHHHHHHGRPMFEKCLQWILHNQTPQGFWAAAGDNISDTDDDVTLDCLLST
                     LACLVALKRWQLAPDMIHKGLEFVNRNTERLVMKQKPSDVPRWFTIMFPAMLELAGASS
                     LRVDFSENLNRILVELSQNRDDILTREEVDEKKQYSPLLLFLEALPAQSYDNDVLKQII
                     DKNLSNDGSLLQSPSATARAYMITGNTRCLSYLHSLTNSCSNGGVPSFYPVDDDLHDLV
                     MVNQLTRSGLTEHLIPEIDHLLLKVQKNYKYKKASPKSLYSIAAELYRDSLAFWLLRVN
                     NHWVSPSIFCWFLDDDEIRDHIETNYEEFAAVLLNVYRATDLMFSGEVQLVEARSFATK
                     NLEKILATGNIHKTNADISSSLHKMIEHELRVPWTARMDHVENRIWIEEIASSALWFGK
                     SSYLRLSCFHKMSLQQLAVKNYTLRQLVYRDELAEVERWSKERGLCDMGFCREKTGYCY
                     YAFAASTCLPWSSDVRLVLTKAAVVITVADDFFDVEGSMVDLEKLTDAVRRWDAEGLGS
                     HSKTIFEALDDLVNEVRLKCFQQNGQDIKNNLQQLWYETFHSWLMEAKWGKGLTSKPSV
                     DVYLGNAMTSIAAHTMVLTASCLLGPGFPVHQLWSQRRHQDITSLLMVLTRLLNDIQSY
                     LKEEDEGKINYVWMYMIENNQASIDDSVRHVQTIINVKKQEFIQRVLSDQHCNLPKSFK
                     QLHFSCLKVFNMFFNSSNIFDTDTDLLLDIHEAFVSPPQVPKFKPHIKPPHQLPATLQP
                     PHQPQQIMVNKKKVEMVYKSYHHPFKVFTLQKKQSSGHGTMNPRASILAGPNIKLCFS"
                     /original_record="part"
     protein_bind    complement(join(4637..4639,4641..4647))
                     /label="SapI"
                     /ApEinfo_fwdcolor="#00FF2C"
                     /ApEinfo_revcolor="#00FF2C"
                     /original_record="part"
     protein_bind    4648..4653
                     /label="HindIII"
                     /ApEinfo_fwdcolor="#00FF2C"
                     /ApEinfo_revcolor="#00FF2C"
                     /original_record="part"
     misc_feature    2002..2021
                     /label="FreeGenes dialout primer"
                     /ApEinfo_fwdcolor="#0048BA"
                     /ApEinfo_revcolor="#0048BA"
     misc_feature    complement(4661..4680)
                     /label="FreeGenes dialout primer"
                     /ApEinfo_fwdcolor="#0048BA"
                     /ApEinfo_revcolor="#0048BA"
     misc_feature    4681..4740
                     /label="FreeGenes barcode"
                     /ApEinfo_fwdcolor="#FF7E00"
                     /ApEinfo_revcolor="#FF7E00"
     protein_bind    join(2022..2027,2029..2032)
                     /label="BsaI"
                     /ApEinfo_fwdcolor="#00FF2C"
                     /ApEinfo_revcolor="#00FF2C"
     protein_bind    complement(join(4650..4653,4655..4660))
                     /label="BsaI"
                     /ApEinfo_fwdcolor="#00FF2C"
                     /ApEinfo_revcolor="#00FF2C"
     protein_bind    4702..4719
                     /label="I-SceI"
                     /ApEinfo_fwdcolor="#4F0080"
                     /ApEinfo_revcolor="#4F0080"
ORIGIN
        1 cgctgaggtg tcaatcgtcg gagccgctga gcaataacta gcataacccc ttggggcctc
       61 taaacgggtc ttgaggggtt ttttgcatgg tcatagctgt ttcctgagag cttggcaggt
      121 gatgacacac attaacaaat ttcgtgagga gtctccagaa gaatgccatt aatttccata
      181 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
      241 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
      301 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
      361 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
      421 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
      481 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
      541 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
      601 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
      661 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
      721 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaaggccta ctattagcaa
      781 caacgatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
      841 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
      901 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
      961 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     1021 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     1081 aatgataccg cgagaaccac gctcaccggc tccagattta tcagcaataa accagccagc
     1141 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     1201 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     1261 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     1321 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     1381 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     1441 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     1501 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     1561 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     1621 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     1681 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     1741 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     1801 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     1861 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     1921 ctgcaccagt cagtaaaacg acggccagta gtcaaaagcc tccgaccgga ggcttttgac
     1981 ttggttcagg tggagtggga gaacgatgat gctcactctc gggtctctaa tgcaattgat
     2041 aaccaatttt tcatcctcct ctagtgaatt gcagttttta gttgacaaag ttaaaagaga
     2101 atctctaagt tcttccagtt ctaatactca aaatttattc ttgagtactt ctccctatga
     2161 tactgcttgg ctcgctttga ttccacatcc tcaccatcat caccaccatg gtagaccaat
     2221 gttcgaaaaa tgtttgcaat ggattttgca taatcaaact ccacagggtt tttgggctgc
     2281 cgctggtgac aatatttcag atacagacga tgacgttact ttagactgtt tgctatctac
     2341 tctagcgtgt ttggttgctt tgaaaagatg gcaactagct ccagatatga ttcacaaggg
     2401 tctggaattt gttaatagaa atactgaaag gttggtaatg aaacaaaagc cctctgatgt
     2461 tccaagatgg ttcactataa tgttcccagc tatgttggag ttggcaggcg cttcttcact
     2521 aagggtagat ttttctgaaa atttgaatag aatattggtt gaactatctc agaacaggga
     2581 tgatattctt actagagagg aagtagacga aaagaaacaa tactctccat tgttattatt
     2641 tttggaagca ttgcctgctc aatcatacga taacgatgtt ttgaagcaaa ttattgacaa
     2701 gaatctatct aatgacggtt ctttattgca atctccatct gctactgctc gtgcttatat
     2761 gataactggc aacactagat gtttatctta tttgcattca ttgacgaact cttgtagcaa
     2821 cggtggtgtt ccatcattct atccagtcga tgatgacttg catgacttag ttatggtcaa
     2881 tcaattaact agatcgggtt taactgaaca tttgatccca gaaatagacc acttgttact
     2941 taaggttcaa aaaaactata agtataagaa ggcttctcca aaatctttgt actctatagc
     3001 tgctgaattg tatagagact ctttagcttt ctggttattg agagttaata atcattgggt
     3061 tagtccatct attttttgtt ggttcttgga tgacgacgaa atcagagatc acattgaaac
     3121 taactacgaa gaatttgcag ctgttttgct aaatgtctat agggctactg accttatgtt
     3181 ctctggtgaa gttcagcttg ttgaggctag gtcatttgct actaagaacc tagaaaatat
     3241 attggccact ggtaatatac acaagactaa tgctgacatt tcatcttcat tgcacaaaat
     3301 gatagaacac gaattaagag tcccatggac tgctagaatg gatcacgttg agaacagaat
     3361 atggatcgaa gagatcgctt catcagcttt atggtttggt aaatcttcct atttgagatt
     3421 gtcatgtttt cataagatgt ctcttcagca attagctgtt aaaaattaca ctttgagaca
     3481 attagtttat agggacgaat tggctgaagt tgagagatgg tctaaggaaa gaggtttgtg
     3541 tgatatgggt ttctgtagag aaaagactgg ttactgttac tatgcttttg ctgcatctac
     3601 ttgtctgcca tggtctagtg acgttcgatt ggtattgact aaggctgctg ttgttataac
     3661 tgttgctgac gatttctttg acgtcgaagg ttctatggtt gatttggaaa agttgactga
     3721 tgctgttagg agatgggacg ctgaaggttt gggtagtcac tctaagacta tctttgaggc
     3781 attggacgac ttggttaatg aagttagatt gaagtgtttc caacagaacg gtcaagatat
     3841 taagaacaac ttacaacaat tgtggtatga aactttccat tcatggctaa tggaagctaa
     3901 gtggggtaag ggtttgactt ccaagccatc tgttgacgtt tacttgggta atgctatgac
     3961 ttctattgct gctcatacta tggtcctaac tgcttcctgt ttattgggtc caggtttccc
     4021 agttcaccaa ctgtggtcac aaagacgtca ccaagacatt acttctttgt tgatggtttt
     4081 gactagattg ttgaatgaca tacaatctta cttgaaggaa gaagatgaag gtaagatcaa
     4141 ctacgtttgg atgtacatga tcgaaaacaa tcaagctagt atagatgact ctgttagaca
     4201 cgttcaaact attatcaacg taaaaaagca agaatttatc caaagagttt tgtctgatca
     4261 acattgtaac ttgccaaagt ctttcaagca attgcatttc tcttgtttga aggtctttaa
     4321 catgttcttc aactcttcta acatatttga taccgatact gacttgttgt tggacattca
     4381 cgaagcgttc gtttctccac cacaagttcc aaagtttaaa cctcatatca aaccacccca
     4441 ccaattgcca gctactttac aacctccaca ccaaccacaa caaataatgg ttaacaagaa
     4501 gaaggttgaa atggtttata agtcttacca tcacccattc aaggttttta ctttgcaaaa
     4561 gaagcaatct tctggtcatg gtactatgaa cccaagagct tctatcttgg ctggtccaaa
     4621 tataaaattg tgtttttctt gaagagcaag ctttgagacc agggctaatt accatcagcg
     4681 ctcctcgtca acattatcta gtagggataa cagggtaatg tactaacccg acccatggtg
//
