LOCUS       Exported     3218 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3218
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1231..1278
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     3106..3122
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      3123..3154
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             1..0
                     /translation="MDKQIFTIIEFNKNIINFQVIKYFNNQEMILYKNSYVSEESEILDKDQIKDSLNVYKFLTNCFTDFEKNSSFSKIKQVGLMLSNSLNITKKVTNYDLKSKDKKTSSTTNLEYIVKKINKLQLYDDTDLKIIDTNLIELQVNNKMIDYQNLKKYYIDNTQNNKLVMTFLELSITNELYFALEKLFWKFYKQILFIKPRIVCLNQLVKDQCQDHKLVVDWNWDEIEVGVFNNNVLLKIFKFSFGINKIIKNLSQSLNISFEMSKDYLFNNLDFSSYNIDNLNILSLWNNSENKLDITNGKQIKQLIKSIISEIYTTINQSILKEQNINNYQIYNFGLISKIVGIDMIFNELKNNHFISNNFIGDIDFEQDSTAFIGASLFFKNPEFPS"
                     /label="MMSYN1_0523"
                     /note=""
     primer_bind     complement(1283..1299)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1366..1954)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(2125..2985)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(2986..3090)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
     CDS             complement(1..0)
                     /label="MMSYN1_0523"
                     /note=""
                     /translation="MDKQIFTIIEFNKNIINFQVIKYFNNQEMILYKNSYVSEESEILDKDQIKDSLNVYKFLTNCFTDFEKNSSFSKIKQVGLMLSNSLNITKKVTNYDLKSKDKKTSSTTNLEYIVKKINKLQLYDDTDLKIIDTNLIELQVNNKMIDYQNLKKYYIDNTQNNKLVMTFLELSITNELYFALEKLFWKFYKQILFIKPRIVCLNQLVKDQCQDHKLVVDWNWDEIEVGVFNNNVLLKIFKFSFGINKIIKNLSQSLNISFEMSKDYLFNNLDFSSYNIDNLNILSLWNNSENKLDITNGKQIKQLIKSIISEIYTTINQSILKEQNINNYQIYNFGLISKIVGIDMIFNELKNNHFISNNFIGDIDFEQDSTAFIGASLFFKNPEFPS"
ORIGIN
        1 ATGGATAAGC AGATTTTCAC AATCATCGAG TTTAATAAAA ATATCATCAA TTTCCAGGTC
       61 ATTAAATACT TCAACAACCA AGAGATGATT TTATACAAGA ACTCTTATGT ATCTGAGGAG
      121 AGTGAAATTC TGGACAAAGA CCAGATTAAA GATTCTCTTA ATGTTTATAA GTTTCTGACA
      181 AATTGCTTCA CGGATTTTGA GAAAAACTCG TCTTTTTCGA AGATTAAACA GGTCGGCTTG
      241 ATGTTATCGA ATTCATTGAA CATCACTAAA AAGGTCACTA ATTACGATTT AAAGTCTAAA
      301 GATAAGAAAA CCTCAAGCAC GACGAATTTA GAATATATTG TTAAGAAAAT CAATAAGTTG
      361 CAGTTATACG ACGATACCGA TTTGAAGATC ATCGATACAA ATTTGATTGA ACTGCAAGTT
      421 AACAATAAAA TGATTGACTA TCAGAACCTG AAAAAATACT ATATCGACAA TACTCAAAAC
      481 AATAAGTTAG TTATGACATT TTTAGAGCTG AGCATCACGA ATGAATTGTA TTTTGCCTTG
      541 GAGAAACTGT TCTGGAAATT TTACAAACAA ATTTTGTTTA TTAAGCCTCG CATCGTATGC
      601 TTAAACCAGC TGGTGAAGGA TCAGTGTCAG GATCACAAAC TGGTCGTTGA CTGGAACTGG
      661 GACGAGATTG AGGTGGGTGT TTTTAATAAC AACGTCCTGC TGAAAATTTT CAAGTTCTCA
      721 TTTGGGATCA ACAAAATTAT CAAAAACTTA TCACAAAGTT TGAATATCAG CTTTGAAATG
      781 TCGAAGGACT ACCTTTTCAA TAACTTGGAT TTTTCTTCGT ACAACATCGA CAATTTGAAT
      841 ATCCTGTCTT TATGGAATAA TAGTGAGAAT AAATTAGACA TCACCAACGG TAAACAGATT
      901 AAGCAGCTTA TTAAAAGTAT TATTAGCGAG ATCTATACAA CGATCAACCA GAGCATCCTG
      961 AAAGAACAAA ATATCAATAA TTATCAGATT TACAACTTTG GCCTGATCTC GAAGATTGTG
     1021 GGTATCGACA TGATTTTTAA CGAACTGAAG AATAACCATT TTATTTCAAA TAACTTTATT
     1081 GGAGATATCG ACTTTGAACA AGACTCAACG GCGTTCATCG GGGCTTCGTT GTTCTTCAAG
     1141 AATCCAGAAT TTCCATCCTG AAGAGCTTAG AGACCGGTAC TAGTTCCTGC AGGGCTCGAG
     1201 GTGTCAATCG TCGGAGCCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG
     1261 GTCTTGAGGG GTTTTTTGCA TGGTCATAGC TGTTTCCTGA GAGCTTGGCA GGTGATGACA
     1321 CACATTAACA AATTTCGTGA GGAGTCTCCA GAAGAATGCC ATTAATTTCC ATAGGCTCCG
     1381 CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG
     1441 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC
     1501 CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA
     1561 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT
     1621 GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC
     1681 CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG
     1741 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC
     1801 TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT
     1861 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA
     1921 GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG
     1981 GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA
     2041 AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT
     2101 ATATGAGTAA ACTTGGTCTG ACAGTTACCA ATGCTTAATC AGTGAGGCAC CTATCTCAGC
     2161 GATCTGTCTA TTTCGTTCAT CCATAGTTGC CTGACTCCCC GTCGTGTAGA TAACTACGAT
     2221 ACGGGAGGGC TTACCATCTG GCCCCAGTGC TGCAATGATA CCGCGAGAAC CACGCTCACC
     2281 GGCTCCAGAT TTATCAGCAA TAAACCAGCC AGCCGGAAGG GCCGAGCGCA GAAGTGGTCC
     2341 TGCAACTTTA TCCGCCTCCA TCCAGTCTAT TAATTGTTGC CGGGAAGCTA GAGTAAGTAG
     2401 TTCGCCAGTT AATAGTTTGC GCAACGTTGT TGCCATTGCT ACAGGCATCG TGGTGTCACG
     2461 CTCGTCGTTT GGTATGGCTT CATTCAGCTC CGGTTCCCAA CGATCAAGGC GAGTTACATG
     2521 ATCCCCCATG TTGTGCAAAA AAGCGGTTAG CTCCTTCGGT CCTCCGATCG TTGTCAGAAG
     2581 TAAGTTGGCC GCAGTGTTAT CACTCATGGT TATGGCAGCA CTGCATAATT CTCTTACTGT
     2641 CATGCCATCC GTAAGATGCT TTTCTGTGAC TGGTGAGTAC TCAACCAAGT CATTCTGAGA
     2701 ATAGTGTATG CGGCGACCGA GTTGCTCTTG CCCGGCGTCA ATACGGGATA ATACCGCGCC
     2761 ACATAGCAGA ACTTTAAAAG TGCTCATCAT TGGAAAACGT TCTTCGGGGC GAAAACTCTC
     2821 AAGGATCTTA CCGCTGTTGA GATCCAGTTC GATGTAACCC ACTCGTGCAC CCAACTGATC
     2881 TTCAGCATCT TTTACTTTCA CCAGCGTTTC TGGGTGAGCA AAAACAGGAA GGCAAAATGC
     2941 CGCAAAAAAG GGAATAAGGG CGACACGGAA ATGTTGAATA CTCATACTCT TCCTTTTTCA
     3001 ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT
     3061 TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACCTGCACC AGTCAGTAAA ACGACGGCCA
     3121 GTAGTCAAAA GCCTCCGACC GGAGGCTTTT GACTTGGTCA GGTGGAGTGA ATTCGCGGCC
     3181 GCTTCTAGAG GCTCTTCGTA ATTACGAAGA GGTCTCTA
//