LOCUS       Exported     2984 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..2984
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      997..1044
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     2872..2888
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      2889..2920
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             1..0
                     /translation="MDKHIPVLLKESIEYLNIKPDGIYVDCTLGRAGHSSEILKKLNQKGFLYAIDQDQIAIDQAKEKLEQINNNFLLIQGNFSNLSALLAINHVFNVDGILYDLGVSSPQIDIASRGFSYKMDGPLDMRMDLNSTLTAHQVINTYSESQISEILFKYGEESFSKSISKKIVESRPINSTLELVEIIKSVLPQKVLKQKKHPAKKTFQALRIYINNELIALENSLKQALDLLNSKARICVITFHSLEEKIVKNIFNNSTNYYQEQLLSNLPIKANLNSKFKLVIKKPIKPSLLELEQNHRSHSAKLWVIEKN"
                     /label="MMSYN1_0524"
                     /note="Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA."
     primer_bind     complement(1049..1065)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1132..1720)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(1891..2751)
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
     promoter        complement(2752..2856)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
     CDS             complement(1..0)
                     /label="MMSYN1_0524"
                     /note="Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA."
                     /translation="MDKHIPVLLKESIEYLNIKPDGIYVDCTLGRAGHSSEILKKLNQKGFLYAIDQDQIAIDQAKEKLEQINNNFLLIQGNFSNLSALLAINHVFNVDGILYDLGVSSPQIDIASRGFSYKMDGPLDMRMDLNSTLTAHQVINTYSESQISEILFKYGEESFSKSISKKIVESRPINSTLELVEIIKSVLPQKVLKQKKHPAKKTFQALRIYINNELIALENSLKQALDLLNSKARICVITFHSLEEKIVKNIFNNSTNYYQEQLLSNLPIKANLNSKFKLVIKKPIKPSLLELEQNHRSHSAKLWVIEKN"
ORIGIN
        1 ATGGATAAGC ATATCCCGGT TCTTTTAAAG GAAAGCATTG AATATCTTAA TATCAAACCC
       61 GACGGCATTT ACGTTGACTG CACCTTGGGA CGCGCGGGCC ATTCTTCAGA GATCCTCAAA
      121 AAGCTGAATC AAAAGGGATT CTTATACGCT ATTGACCAGG ATCAAATTGC GATCGACCAA
      181 GCTAAAGAAA AGTTGGAACA AATCAACAAT AATTTCCTGT TGATTCAAGG TAACTTTTCT
      241 AACCTTAGTG CTCTGTTGGC TATTAACCAT GTTTTTAACG TTGACGGTAT CCTGTATGAT
      301 TTGGGGGTAT CCAGCCCACA GATCGACATT GCATCTCGTG GGTTCTCCTA CAAAATGGAT
      361 GGACCCTTAG ACATGCGTAT GGACCTGAAT AGTACGCTGA CTGCGCATCA AGTCATCAAT
      421 ACCTATTCGG AATCGCAAAT TTCGGAGATC TTGTTTAAGT ATGGGGAGGA AAGCTTCTCG
      481 AAGAGTATCT CCAAGAAGAT CGTGGAGAGT CGCCCGATCA ATAGCACGTT GGAACTTGTA
      541 GAAATTATTA AATCAGTGCT TCCGCAAAAA GTACTTAAAC AGAAGAAACA CCCTGCCAAA
      601 AAGACGTTTC AGGCATTACG TATCTATATC AACAACGAAC TTATCGCCTT GGAAAATTCA
      661 CTGAAGCAGG CTTTGGATCT GTTAAATAGT AAGGCCCGTA TTTGTGTGAT TACCTTCCAC
      721 AGTCTGGAAG AAAAGATCGT CAAAAATATC TTCAACAATA GCACGAACTA CTATCAGGAA
      781 CAGTTATTGT CAAATCTTCC TATCAAGGCT AACTTGAACA GTAAGTTCAA GTTAGTAATC
      841 AAGAAGCCCA TTAAGCCTTC TCTTCTGGAG TTAGAGCAAA ACCACCGCTC ACACTCAGCG
      901 AAATTGTGGG TAATTGAGAA AAACTGAAGA GCTTAGAGAC CGGTACTAGT TCCTGCAGGG
      961 CTCGAGGTGT CAATCGTCGG AGCCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT
     1021 AAACGGGTCT TGAGGGGTTT TTTGCATGGT CATAGCTGTT TCCTGAGAGC TTGGCAGGTG
     1081 ATGACACACA TTAACAAATT TCGTGAGGAG TCTCCAGAAG AATGCCATTA ATTTCCATAG
     1141 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC
     1201 GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT
     1261 TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT
     1321 TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG
     1381 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT
     1441 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT
     1501 TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG
     1561 CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA
     1621 AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT
     1681 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC
     1741 TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT
     1801 ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA
     1861 AAGTATATAT GAGTAAACTT GGTCTGACAG TTACCAATGC TTAATCAGTG AGGCACCTAT
     1921 CTCAGCGATC TGTCTATTTC GTTCATCCAT AGTTGCCTGA CTCCCCGTCG TGTAGATAAC
     1981 TACGATACGG GAGGGCTTAC CATCTGGCCC CAGTGCTGCA ATGATACCGC GAGAACCACG
     2041 CTCACCGGCT CCAGATTTAT CAGCAATAAA CCAGCCAGCC GGAAGGGCCG AGCGCAGAAG
     2101 TGGTCCTGCA ACTTTATCCG CCTCCATCCA GTCTATTAAT TGTTGCCGGG AAGCTAGAGT
     2161 AAGTAGTTCG CCAGTTAATA GTTTGCGCAA CGTTGTTGCC ATTGCTACAG GCATCGTGGT
     2221 GTCACGCTCG TCGTTTGGTA TGGCTTCATT CAGCTCCGGT TCCCAACGAT CAAGGCGAGT
     2281 TACATGATCC CCCATGTTGT GCAAAAAAGC GGTTAGCTCC TTCGGTCCTC CGATCGTTGT
     2341 CAGAAGTAAG TTGGCCGCAG TGTTATCACT CATGGTTATG GCAGCACTGC ATAATTCTCT
     2401 TACTGTCATG CCATCCGTAA GATGCTTTTC TGTGACTGGT GAGTACTCAA CCAAGTCATT
     2461 CTGAGAATAG TGTATGCGGC GACCGAGTTG CTCTTGCCCG GCGTCAATAC GGGATAATAC
     2521 CGCGCCACAT AGCAGAACTT TAAAAGTGCT CATCATTGGA AAACGTTCTT CGGGGCGAAA
     2581 ACTCTCAAGG ATCTTACCGC TGTTGAGATC CAGTTCGATG TAACCCACTC GTGCACCCAA
     2641 CTGATCTTCA GCATCTTTTA CTTTCACCAG CGTTTCTGGG TGAGCAAAAA CAGGAAGGCA
     2701 AAATGCCGCA AAAAAGGGAA TAAGGGCGAC ACGGAAATGT TGAATACTCA TACTCTTCCT
     2761 TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA
     2821 ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACC TGCACCAGTC AGTAAAACGA
     2881 CGGCCAGTAG TCAAAAGCCT CCGACCGGAG GCTTTTGACT TGGTCAGGTG GAGTGAATTC
     2941 GCGGCCGCTT CTAGAGGCTC TTCGTAATTA CGAAGAGGTC TCTA
//