LOCUS       Exported     4408 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..4408
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      38..85
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     1933..1949
                     /note="common sequencing primer, one of multiple similar variants"
                     /label="M13 for"
     terminator      1950..1981
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             2010..4394
                     /note=""
                     /translation="MIITRNWLKKYLNLDNISNDQINMALNSLGFEVDSVYDLNSLNSELILGYVEQSKQIPNTHLKLNQVNIGTKSLQIVCGASNVDVNQFVIIAPINATIANGLTLTSKKIQNYESQGMICALNEIGIDLSVINKEDQLKIYNVFDHNLDLKKYIGSDVKQIIGLDDYLFEIDLTLNRSDCLASFQILKELANYFDLEIKNLDNNFSDFKKNNLDLKIDLVNQVKDQIKTISYSYFELNNKNDKLDSKDEIFLKLNQINSSNHLITNLSLISTLSTAQTHILIDLDKLKSSNLKLEFINHDNKELLCLTNNNKLVNIIGLDTQNEFNVDNNSKNVLNIMLNIEPNLMRKQQKLLNISNTYLQRYIKPINPNLFNLANQTFSNLLNDYQLINKAYEVKILKQTFKNKQSLEIKLNEINDLLGTNLTIKQIKSLFKHLDFKITNKDDLLDFQIDQNRIDITSKNDLCEEVARLYSYDKIDEVPLSFTSFKKAKNLNLKLENKLTNYLIGLGFNNTKTYSLTSLNEAKYWNLFNISEFINLVSPLSNLRQTYRTNLSKSLIDVAIFNHSINNKELKLFEIADIYDLNNLKQRHLVFLTSNHIYKNSLNQQLIENNFYYNKEILENIFNLYNLDLSEIQYQNDLNLIKEIHPYINTTIYYQNQLIGYLYKLNPKFESENKLNPTFVCEINLDILNQFKNSFIEAKTLSKFQSSSRDLTIDISNDLTYQKVLFNALSDVKYLKSHKIVDLYLDDNLIKNNTKALTIQFVFNDLDHQLTENEINQEFEKIIKNIKQMKVVIR"
                     /label="MMSYN1_0528"
     primer_bind     complement(90..106)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(173..761)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(952..1812)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(1813..1917)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC
       61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT
      121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA
      181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC
      241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG
      301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC
      361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG
      421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC
      481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA
      541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG
      601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA
      661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG
      721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA
      781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA
      841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA
      901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG
      961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG
     1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC
     1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC
     1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA
     1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC
     1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG
     1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC
     1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT
     1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG
     1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC
     1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG
     1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT
     1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG
     1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG
     1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT
     1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC
     1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC
     1981 TTGGTTCAGG TGGAGTGGGA GGGTCTCAAA TGATTATTAC GCGGAACTGG TTGAAAAAAT
     2041 ATCTGAATCT GGACAACATT TCCAATGACC AGATTAATAT GGCTCTTAAC TCCCTTGGGT
     2101 TCGAAGTCGA CTCCGTGTAC GATCTGAATA GCCTTAACAG CGAATTAATT TTAGGGTACG
     2161 TTGAGCAATC GAAACAGATA CCTAATACCC ATTTAAAGCT GAATCAAGTC AACATCGGTA
     2221 CCAAGAGTCT GCAGATTGTC TGTGGCGCCT CTAATGTAGA TGTAAACCAG TTCGTGATAA
     2281 TCGCCCCGAT TAACGCGACG ATCGCTAACG GGTTAACGTT GACAAGCAAA AAGATCCAAA
     2341 ACTACGAAAG TCAAGGTATG ATCTGTGCCC TGAACGAAAT AGGCATAGAC TTATCTGTAA
     2401 TTAATAAAGA GGACCAATTA AAGATATACA ACGTTTTCGA CCATAACTTA GACTTAAAGA
     2461 AATACATAGG CAGTGATGTT AAACAGATCA TCGGGCTTGA CGATTATCTT TTTGAAATAG
     2521 ACCTTACTCT TAACCGGAGC GACTGCTTGG CCAGCTTCCA GATACTTAAA GAGTTGGCCA
     2581 ACTATTTCGA TTTAGAGATA AAGAATCTTG ATAACAACTT CTCTGATTTC AAAAAGAACA
     2641 ATTTAGACCT TAAAATTGAC TTAGTCAACC AGGTCAAGGA CCAGATCAAA ACCATCTCCT
     2701 ACAGCTATTT CGAGCTTAAC AATAAGAACG ACAAGTTAGA CTCTAAAGAC GAAATTTTCC
     2761 TGAAGTTAAA CCAAATCAAT TCCAGTAACC ATCTTATAAC GAATTTGTCT TTGATATCAA
     2821 CTTTATCAAC CGCACAGACC CACATCCTTA TAGATTTGGA TAAGCTGAAG TCCTCTAATT
     2881 TAAAGTTGGA ATTTATAAAT CACGACAATA AAGAGCTGTT GTGTTTGACC AATAACAATA
     2941 AACTTGTGAA CATAATTGGG TTAGATACCC AGAATGAGTT CAACGTAGAT AATAACAGCA
     3001 AAAACGTATT GAACATTATG CTGAATATAG AACCAAATTT GATGCGGAAA CAGCAGAAAC
     3061 TGCTTAATAT CTCAAACACC TACTTGCAGC GCTACATCAA ACCGATCAAC CCTAATTTGT
     3121 TTAACTTAGC AAATCAGACC TTCTCAAATT TATTAAATGA TTACCAACTG ATCAACAAGG
     3181 CTTATGAAGT AAAGATCCTG AAGCAAACAT TCAAAAACAA ACAGTCACTG GAAATCAAGC
     3241 TTAACGAAAT CAACGACTTA TTGGGAACTA ACTTAACGAT CAAGCAAATC AAGAGTCTTT
     3301 TTAAACACCT GGATTTTAAG ATTACCAATA AAGACGACCT TCTGGATTTC CAGATAGACC
     3361 AGAATCGTAT TGACATCACC AGCAAAAATG ACCTTTGCGA GGAGGTGGCT CGTTTATATA
     3421 GCTATGACAA AATTGACGAA GTTCCACTTT CGTTTACTTC GTTCAAGAAA GCGAAAAACC
     3481 TGAACCTTAA GTTAGAGAAT AAATTGACTA ATTACCTGAT CGGACTTGGA TTCAACAATA
     3541 CCAAGACATA CTCATTGACC TCCCTGAACG AAGCAAAGTA CTGGAATCTT TTCAACATCT
     3601 CAGAATTTAT AAATCTGGTA TCTCCGCTGT CAAATCTTCG TCAAACATAT AGAACAAACC
     3661 TTTCAAAATC GCTTATTGAC GTCGCAATCT TTAACCACTC AATAAACAAC AAGGAGTTAA
     3721 AGCTTTTCGA AATCGCGGAT ATATACGACT TGAACAATCT GAAGCAACGT CATCTGGTAT
     3781 TTTTAACCAG TAACCACATC TATAAAAACT CCCTTAATCA GCAATTGATA GAAAACAACT
     3841 TCTATTACAA TAAGGAAATT CTTGAAAACA TCTTTAATCT TTATAACCTT GACCTGAGTG
     3901 AAATTCAGTA CCAGAATGAT TTAAATCTGA TTAAAGAGAT CCATCCCTAT ATTAATACTA
     3961 CTATATACTA CCAGAACCAG CTTATAGGTT ATTTGTATAA GCTTAACCCG AAGTTCGAAT
     4021 CGGAGAATAA ACTTAACCCG ACGTTCGTGT GTGAAATAAA TCTGGATATC TTAAACCAAT
     4081 TCAAGAATTC ATTCATTGAA GCTAAGACAC TTTCAAAATT CCAATCCTCC TCTAGAGACT
     4141 TAACAATAGA CATATCGAAT GACTTGACAT ATCAAAAAGT GCTTTTCAAC GCTTTGAGTG
     4201 ACGTGAAATA TCTTAAGTCA CATAAGATTG TCGATTTATA CCTGGATGAT AACTTAATAA
     4261 AAAATAATAC AAAAGCATTG ACTATACAAT TTGTATTCAA TGATCTGGAT CACCAACTGA
     4321 CAGAGAACGA GATAAATCAG GAGTTTGAAA AGATAATTAA AAACATTAAA CAGATGAAGG
     4381 TAGTCATCCG CTGAAGAGCT TAGAGACC
//