LOCUS       Exported     3110 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3110
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
                     /label="T7 terminator"
     terminator      1123..1170
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     2998..3014
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      3015..3046
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             1..0
                     /note=""
                     /translation="MIEQLNEILNNFKVKLELVKDLNSWEELKKEFIGKDSSLTTILKSIKTISSDKKQEIGKLANQIRVEIINQLNEKQEQLKNKELLVKLEKEKIDVTLTNSSLKFGSKHVLNIVIEEISDIFTEIGFEMVSGTEIESDLYNFQKLNLPVDHPARDMQDTFYLDNNLVLRTHCTNMTSRMLTKLASLKTEDNNLAVISYGNVYRRDDDDATHSHQFMQIDGFVVGNKISFANLKWILKYMCQRLFNKDINIRLRPSYFPFTEPSVEVDVSCFKCDSKGCFICKKTGWIEILGAGMINEHVLKLNGLDPTKCSGLAFGIGIERIAMLKFNISNIRNFYENNVKFLEQFKFYSE"
                     /label="MMSYN1_0529"
     primer_bind     complement(1175..1191)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1258..1846)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(2017..2877)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(2878..2982)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
     CDS             complement(1..0)
                     /label="MMSYN1_0529"
                     /note=""
                     /translation="MIEQLNEILNNFKVKLELVKDLNSWEELKKEFIGKDSSLTTILKSIKTISSDKKQEIGKLANQIRVEIINQLNEKQEQLKNKELLVKLEKEKIDVTLTNSSLKFGSKHVLNIVIEEISDIFTEIGFEMVSGTEIESDLYNFQKLNLPVDHPARDMQDTFYLDNNLVLRTHCTNMTSRMLTKLASLKTEDNNLAVISYGNVYRRDDDDATHSHQFMQIDGFVVGNKISFANLKWILKYMCQRLFNKDINIRLRPSYFPFTEPSVEVDVSCFKCDSKGCFICKKTGWIEILGAGMINEHVLKLNGLDPTKCSGLAFGIGIERIAMLKFNISNIRNFYENNVKFLEQFKFYSE"
ORIGIN
        1 ATGATCGAGC AATTGAACGA GATCCTTAAT AACTTTAAGG TTAAATTAGA GCTGGTCAAA
       61 GATCTTAATT CGTGGGAGGA GCTGAAAAAG GAGTTCATCG GAAAAGACTC AAGTCTTACC
      121 ACCATCCTGA AGTCAATTAA GACAATCTCA TCCGATAAGA AACAGGAGAT TGGCAAACTG
      181 GCTAATCAGA TTCGCGTCGA GATTATTAAC CAATTAAACG AAAAACAAGA GCAACTTAAA
      241 AATAAGGAAC TTTTGGTGAA ATTAGAAAAA GAGAAAATTG ACGTTACGTT AACCAACTCC
      301 TCATTAAAGT TTGGTAGTAA ACATGTATTG AACATCGTTA TTGAAGAAAT TTCAGATATT
      361 TTCACGGAGA TCGGGTTCGA GATGGTGAGC GGTACTGAAA TCGAGTCGGA TCTTTACAAC
      421 TTCCAAAAAT TAAATCTGCC CGTAGATCAT CCGGCTCGTG ATATGCAGGA TACTTTCTAC
      481 TTGGATAACA ATCTGGTTTT ACGTACTCAT TGTACGAATA TGACTTCCCG CATGTTGACA
      541 AAGCTGGCAT CTCTTAAGAC AGAAGATAAT AATTTAGCCG TAATTTCATA TGGTAACGTA
      601 TACCGCCGCG ACGATGACGA CGCCACGCAC AGCCATCAGT TCATGCAAAT CGATGGCTTC
      661 GTCGTTGGCA ACAAAATTAG CTTCGCTAAC TTGAAGTGGA TCCTGAAGTA CATGTGCCAG
      721 CGTTTGTTCA ATAAAGACAT TAACATTCGT TTGCGTCCTT CTTATTTTCC TTTTACTGAA
      781 CCATCCGTTG AAGTGGATGT AAGTTGTTTC AAGTGCGATT CTAAGGGCTG TTTTATCTGC
      841 AAAAAGACTG GCTGGATCGA AATTTTAGGG GCCGGAATGA TTAATGAGCA TGTTCTTAAA
      901 CTTAATGGGT TAGACCCTAC TAAGTGCTCG GGACTGGCCT TTGGGATCGG TATTGAGCGT
      961 ATCGCCATGC TTAAGTTTAA CATCTCCAAT ATCCGCAACT TCTATGAGAA TAACGTTAAA
     1021 TTCCTTGAAC AGTTTAAATT TTACAGTGAG TGAAGAGCTT AGAGACCGGT ACTAGTTCCT
     1081 GCAGGGCTCG AGGTGTCAAT CGTCGGAGCC GCTGAGCAAT AACTAGCATA ACCCCTTGGG
     1141 GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CATGGTCATA GCTGTTTCCT GAGAGCTTGG
     1201 CAGGTGATGA CACACATTAA CAAATTTCGT GAGGAGTCTC CAGAAGAATG CCATTAATTT
     1261 CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG
     1321 AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC
     1381 TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT
     1441 GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA
     1501 GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA
     1561 TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA
     1621 CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA
     1681 CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT
     1741 CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT
     1801 TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT
     1861 CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT
     1921 GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC
     1981 AATCTAAAGT ATATATGAGT AAACTTGGTC TGACAGTTAC CAATGCTTAA TCAGTGAGGC
     2041 ACCTATCTCA GCGATCTGTC TATTTCGTTC ATCCATAGTT GCCTGACTCC CCGTCGTGTA
     2101 GATAACTACG ATACGGGAGG GCTTACCATC TGGCCCCAGT GCTGCAATGA TACCGCGAGA
     2161 ACCACGCTCA CCGGCTCCAG ATTTATCAGC AATAAACCAG CCAGCCGGAA GGGCCGAGCG
     2221 CAGAAGTGGT CCTGCAACTT TATCCGCCTC CATCCAGTCT ATTAATTGTT GCCGGGAAGC
     2281 TAGAGTAAGT AGTTCGCCAG TTAATAGTTT GCGCAACGTT GTTGCCATTG CTACAGGCAT
     2341 CGTGGTGTCA CGCTCGTCGT TTGGTATGGC TTCATTCAGC TCCGGTTCCC AACGATCAAG
     2401 GCGAGTTACA TGATCCCCCA TGTTGTGCAA AAAAGCGGTT AGCTCCTTCG GTCCTCCGAT
     2461 CGTTGTCAGA AGTAAGTTGG CCGCAGTGTT ATCACTCATG GTTATGGCAG CACTGCATAA
     2521 TTCTCTTACT GTCATGCCAT CCGTAAGATG CTTTTCTGTG ACTGGTGAGT ACTCAACCAA
     2581 GTCATTCTGA GAATAGTGTA TGCGGCGACC GAGTTGCTCT TGCCCGGCGT CAATACGGGA
     2641 TAATACCGCG CCACATAGCA GAACTTTAAA AGTGCTCATC ATTGGAAAAC GTTCTTCGGG
     2701 GCGAAAACTC TCAAGGATCT TACCGCTGTT GAGATCCAGT TCGATGTAAC CCACTCGTGC
     2761 ACCCAACTGA TCTTCAGCAT CTTTTACTTT CACCAGCGTT TCTGGGTGAG CAAAAACAGG
     2821 AAGGCAAAAT GCCGCAAAAA AGGGAATAAG GGCGACACGG AAATGTTGAA TACTCATACT
     2881 CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT
     2941 ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACCTGCA CCAGTCAGTA
     3001 AAACGACGGC CAGTAGTCAA AAGCCTCCGA CCGGAGGCTT TTGACTTGGT CAGGTGGAGT
     3061 GAATTCGCGG CCGCTTCTAG AGGCTCTTCG TAATTACGAA GAGGTCTCTA
//