LOCUS       Exported     6472 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..6472
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      38..85
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     1933..1949
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      1950..1981
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             2010..6458
                     /translation="MRHIIKSYLKTFFKKNYVSTFGILLFIITLATVIIGMLATPLQLNNRINYLAKHNTSYNSILDTRSMNYDPKFTYNYFYLNKEINNKDTNYTKLSELYIKAINSELEQNFTNTSTDKKENNLYIYDSNNLEDRVKIDFIGNLINSDLFRYRNGALIKTESYIFNKDYNNDQNNLNSFSNISNQVLNRIISDFHQSMSDGISLDNNAKYDYVVSEFYKAYSRFNSFLTINEINLIDKPILTFKFTEILNKLNDNKIDEITKFLVKQLQDLKNKIKNHQKERIYLPSFLVFSDKFSKVLANEKFLYDDRIYIVDQLLDNVENFVLQTKKTFKIQQSSVGQLLPFLTLQLTSDNQIFKNTNKDFNQIQFDKNHKNSEFAKKWDVNINYQQKVNPTQIVISSSYAKARNLKINDEFIIPSSNISDIYLSLINKKDAYYLGSINSKIVGIGSTFDDIVSKNSATDYFQDKTSYVVGYTSKEFINSIRNSRWNFSNKFDTSYQVNFRVKNLNNSTSKDLNKHFIIKFDNWSDESYSVFDKSSSLITEWYSLRTSQAISSIKVQVIIYIVIGIFVLLLSFVFINFALKKEMNETRRQIGIFKSFGYKVVELSWIFALKTWLTMFFGLIIGYILSIPIQIYSSSNFVNSVTFTFNSIYISPLLIIFLIIIIPFIFLMGSYWASIIYIKEPVLSLMNNLKKSKRTKSGAITNLLSKHNIGFNYRMRLSFIKNAKGKFAVVQILFGFASLTYTLLFVAQAILFQSINQSLATIKQDVITKSMWNVNKKIDNTSTNDKLSYTNKNDPKTRQTLSYHDLNKKNINTYLNNDLKQTDIRYRVELFLKLLNNTFNSLSNEKKVSMILPLDYAKKTLTPFLQPGKTDKNDYEVLTKDNQYYLSYISRFNLYNQNQKWQSALNDFKNNKEIKLTLNDLSQKQHSSDLFYDLNHPKKDELQNTIIGLQSTRNNSNNTLFLSSFAKIFSYKLVQAYSLFQVVNHYKQFNNDINKAWMHLQKDNDLLSFNPDDQKYWTIANNPLLEKIINKNLKNKPNKDKKELFDTTSNFSIDSLLNSTNLSNASQSILLASMIMQDLNNKLENNPIVSFNQMFYDSSTDLLSAVIRVSNSDILNPGSYALNLYRLKDHNFGDVNQFLNFKGVSIKGFQDLSKLPEKHNNLPTFNVIVPYYYAKSKNLDINSKIVVETRTTFVKKFVLNVVGINKSETLSISKTPDIFLDYDLFANEMFSEDLYKNNNPLIFNQLWSKNKILEGTINFTKLDDSFKTIKYYGNNLAIDIRKDAPIFLSMYSNIFNEFNNFISKYQELDQQNDIYNTPNPAITTLSRLNSKLFNFNLVKQTISKITTITNQVMLLFILLVSLLLTIILVVVMNIVVDESKKTILTLRAIGYENSEVNWIVMGSYIIGAIISFIIAYLLSNLIWWSFLYYVSYKWHIYIFLAFDFKTLFVTFSVIAFVLFIGWLFSDKQVKKTAITQVTQAE"
                     /label="MMSYN1_0531"
                     /note=""
     primer_bind     complement(90..106)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(173..761)
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
                     /label="ori"
     CDS             complement(952..1812)
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
     promoter        complement(1813..1917)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC
       61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT
      121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA
      181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC
      241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG
      301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC
      361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG
      421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC
      481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA
      541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG
      601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA
      661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG
      721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA
      781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA
      841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA
      901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG
      961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG
     1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC
     1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC
     1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA
     1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC
     1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG
     1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC
     1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT
     1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG
     1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC
     1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG
     1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT
     1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG
     1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG
     1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT
     1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC
     1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC
     1981 TTGGTTCAGG TGGAGTGGGA GGGTCTCAAA TGCGCCATAT CATTAAGTCA TATTTAAAGA
     2041 CATTCTTCAA GAAAAATTAC GTGAGTACCT TTGGCATTCT GCTTTTTATT ATTACCCTGG
     2101 CCACTGTCAT TATCGGAATG TTGGCAACCC CGCTTCAATT GAATAACCGC ATCAATTATC
     2161 TTGCGAAGCA CAATACCTCC TACAATAGCA TCTTAGACAC CCGCAGCATG AATTACGACC
     2221 CTAAATTTAC GTATAACTAT TTCTACTTAA ATAAAGAAAT CAACAACAAG GATACGAATT
     2281 ACACAAAGTT GTCAGAGCTT TACATTAAGG CGATTAATTC GGAGTTGGAG CAGAATTTTA
     2341 CAAACACGAG TACCGATAAA AAGGAGAACA ACCTGTATAT TTACGACTCA AATAATTTAG
     2401 AAGATCGCGT AAAAATTGAC TTCATTGGAA ATTTGATCAA CTCCGATTTA TTTCGTTATC
     2461 GTAACGGGGC CTTGATCAAA ACAGAATCGT ATATCTTCAA CAAAGATTAT AACAACGACC
     2521 AAAACAATCT GAACAGTTTC AGCAACATTA GCAACCAGGT ACTTAACCGT ATCATTAGCG
     2581 ATTTCCACCA ATCAATGTCA GATGGCATTT CGCTGGACAA CAACGCCAAA TACGACTACG
     2641 TTGTGAGTGA GTTTTACAAG GCTTATTCCC GTTTTAACTC GTTTCTGACT ATCAATGAGA
     2701 TCAACCTGAT CGATAAACCC ATTTTAACGT TCAAGTTCAC GGAGATTCTG AATAAGTTGA
     2761 ATGATAATAA AATCGACGAA ATTACTAAAT TCTTAGTAAA ACAGCTTCAG GACTTGAAAA
     2821 ATAAGATTAA GAACCACCAA AAGGAACGCA TTTATCTGCC CTCTTTTTTA GTATTTTCAG
     2881 ATAAATTTAG CAAGGTCCTG GCTAACGAGA AGTTTCTGTA CGACGATCGT ATCTATATCG
     2941 TGGATCAATT ACTGGACAAC GTAGAAAACT TCGTGTTGCA GACCAAGAAA ACCTTTAAAA
     3001 TCCAACAAAG TTCAGTAGGG CAGCTGTTAC CTTTTCTTAC GTTACAATTG ACCTCCGACA
     3061 ACCAGATTTT CAAAAATACG AATAAGGATT TTAATCAAAT CCAATTTGAC AAAAATCATA
     3121 AGAATTCTGA GTTCGCGAAA AAATGGGATG TAAACATTAA CTATCAACAG AAGGTTAATC
     3181 CTACACAAAT CGTTATCTCT AGCAGTTACG CGAAAGCGCG TAACTTGAAA ATCAACGATG
     3241 AATTTATTAT TCCTTCCTCA AACATCAGCG ATATCTACTT ATCCCTTATT AATAAGAAAG
     3301 ATGCCTACTA TCTGGGAAGC ATCAACAGTA AAATCGTTGG CATTGGCTCT ACGTTCGATG
     3361 ATATTGTCAG TAAGAACTCG GCAACTGATT ACTTTCAGGA TAAGACATCT TATGTTGTCG
     3421 GGTACACCTC CAAGGAATTT ATCAATTCCA TTCGTAACTC GCGTTGGAAT TTTTCAAACA
     3481 AATTTGATAC CTCCTATCAA GTGAACTTTC GCGTCAAAAA TTTGAACAAC AGTACAAGCA
     3541 AGGACTTGAA TAAGCACTTT ATCATTAAGT TTGACAATTG GTCGGATGAG AGCTACAGTG
     3601 TATTTGATAA GAGTAGTTCT TTAATCACGG AGTGGTATTC GCTGCGTACT TCACAGGCGA
     3661 TCTCCTCTAT CAAAGTCCAA GTCATCATTT ACATCGTCAT TGGTATCTTT GTGCTGTTAC
     3721 TGTCTTTTGT GTTCATCAAC TTCGCGTTAA AGAAAGAGAT GAATGAAACG CGTCGCCAAA
     3781 TCGGGATCTT CAAGTCCTTC GGGTACAAGG TCGTCGAACT TAGCTGGATC TTCGCTTTAA
     3841 AAACGTGGTT AACGATGTTT TTCGGATTAA TTATCGGCTA TATCCTGTCG ATTCCGATCC
     3901 AGATCTACTC ATCTTCGAAC TTTGTAAATA GCGTCACTTT CACTTTTAAC AGCATTTACA
     3961 TCTCTCCTTT ATTGATCATT TTTCTTATCA TCATTATCCC CTTTATCTTC TTAATGGGCT
     4021 CGTACTGGGC GTCAATCATT TATATCAAGG AGCCGGTGTT GAGCTTGATG AACAATCTGA
     4081 AGAAATCGAA GCGTACTAAA TCTGGAGCGA TTACAAACCT GTTATCCAAG CATAATATCG
     4141 GATTTAACTA TCGTATGCGC CTTTCCTTCA TTAAGAATGC CAAGGGCAAG TTTGCCGTAG
     4201 TTCAGATCTT ATTTGGCTTC GCGTCACTGA CATATACGCT TTTGTTTGTC GCGCAAGCGA
     4261 TTCTGTTCCA GTCCATCAAC CAATCACTGG CCACTATCAA ACAAGATGTC ATTACAAAGT
     4321 CTATGTGGAA TGTCAACAAA AAGATCGATA ACACTTCGAC GAATGATAAG TTAAGTTACA
     4381 CTAATAAGAA CGACCCGAAA ACGCGCCAAA CATTGTCGTA CCATGATTTG AACAAAAAGA
     4441 ATATCAACAC GTACCTTAAC AACGATCTGA AGCAGACTGA TATTCGTTAT CGCGTGGAGT
     4501 TGTTCTTAAA ACTGCTTAAC AACACCTTCA ATTCACTGTC TAATGAAAAG AAAGTTTCCA
     4561 TGATTCTTCC GCTTGACTAC GCGAAGAAAA CTTTAACCCC CTTCCTTCAG CCAGGTAAGA
     4621 CTGATAAAAA TGACTATGAA GTATTAACGA AAGATAATCA GTACTACCTT TCGTATATTA
     4681 GCCGCTTCAA TCTTTATAAC CAGAATCAGA AGTGGCAAAG TGCCTTGAAT GATTTCAAGA
     4741 ATAACAAGGA AATTAAGTTA ACACTGAACG ACCTGTCGCA GAAGCAGCAT TCGAGCGATC
     4801 TTTTCTACGA TTTGAATCAT CCTAAGAAAG ACGAGTTGCA AAATACAATT ATTGGATTAC
     4861 AATCAACCCG TAACAACTCT AATAATACAC TGTTCCTTTC GTCCTTCGCG AAGATCTTTT
     4921 CGTACAAATT GGTCCAAGCA TACTCATTGT TTCAGGTCGT AAACCATTAC AAACAATTTA
     4981 ACAACGACAT TAACAAAGCT TGGATGCACC TTCAAAAGGA CAATGATTTA TTATCCTTTA
     5041 ATCCGGATGA TCAAAAGTAT TGGACAATTG CTAATAATCC ACTTCTTGAA AAGATCATCA
     5101 ATAAAAATTT GAAGAACAAG CCCAATAAAG ATAAAAAGGA GTTATTTGAC ACTACGTCGA
     5161 ATTTCTCAAT TGACTCGTTA CTGAATAGCA CAAACCTTTC AAACGCCTCG CAATCGATCT
     5221 TACTGGCCTC TATGATTATG CAGGATCTTA ACAATAAGCT GGAGAACAAT CCCATTGTCT
     5281 CTTTTAACCA AATGTTCTAC GATAGCAGTA CAGATTTATT GTCCGCAGTT ATCCGTGTTA
     5341 GCAACTCTGA CATCCTGAAT CCAGGGAGCT ACGCCTTGAA TCTTTACCGT TTGAAAGACC
     5401 ACAACTTTGG AGATGTAAAC CAATTTCTTA ATTTTAAGGG AGTTAGTATC AAGGGGTTTC
     5461 AAGATTTATC CAAGTTACCA GAGAAGCATA ATAATCTGCC GACTTTTAAC GTGATTGTGC
     5521 CATATTATTA CGCGAAGTCA AAAAACCTGG ATATCAATAG CAAAATCGTA GTGGAGACTC
     5581 GCACAACATT TGTGAAAAAA TTCGTGTTAA ACGTCGTTGG AATCAATAAG TCAGAAACCC
     5641 TTTCAATTTC TAAGACACCC GATATCTTCT TAGATTATGA CCTTTTCGCG AATGAGATGT
     5701 TCAGTGAGGA TCTTTACAAG AACAATAACC CCTTAATTTT CAATCAATTG TGGAGTAAAA
     5761 ACAAGATCCT GGAAGGTACG ATCAATTTCA CTAAGTTGGA CGACTCATTT AAAACAATTA
     5821 AATACTATGG AAACAATTTG GCAATTGATA TTCGTAAAGA CGCTCCAATC TTCCTGTCAA
     5881 TGTATAGTAA TATCTTCAAT GAGTTTAACA ACTTTATTTC AAAGTATCAG GAACTGGATC
     5941 AACAAAATGA TATCTACAAT ACACCCAACC CCGCAATTAC GACACTGTCG CGTCTTAACT
     6001 CCAAATTATT CAATTTCAAC CTGGTAAAAC AGACGATCTC CAAGATTACT ACCATCACAA
     6061 ACCAGGTAAT GTTGTTGTTT ATCTTACTTG TGTCTTTACT TCTGACTATT ATCTTAGTGG
     6121 TCGTCATGAA CATCGTTGTG GATGAGTCAA AGAAAACAAT TCTTACACTG CGCGCTATTG
     6181 GTTATGAGAA TTCGGAAGTA AATTGGATTG TGATGGGATC CTATATCATT GGTGCGATTA
     6241 TTAGCTTTAT TATTGCGTAT CTGCTGTCGA ACCTGATCTG GTGGAGCTTC CTGTATTATG
     6301 TTAGTTACAA ATGGCACATT TACATCTTTC TTGCTTTTGA CTTCAAAACC TTATTCGTGA
     6361 CTTTCAGTGT TATCGCGTTC GTCCTTTTTA TCGGGTGGCT TTTCTCTGAT AAACAAGTTA
     6421 AAAAGACGGC TATTACTCAA GTGACCCAAG CTGAGTGAAG AGCTTAGAGA CC
//