LOCUS       Exported     3688 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3688
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      38..85
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     1933..1949
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      1950..1981
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             2010..3674
                     /label="MMSYN1_0535"
                     /note="ArgRS: Arginyl-tRNA synthetase"
                     /translation="MSTTIIEMFYNDLKNICQKLNITKEPIIEINKNNTPGLLSSSICLISSKQVNKKPLELAEIFKQELLLTNSYLNIDIAAPGFLNVLVKPEILSNVISNVLSLKSKYGNLEKQNKTINIEYVSANPTGYLHVGHARNAVIGSVLVNLFKKAGYNVQTEYYVNDAGNQINVLAVTVFVHYLQALNIDAKKPENCYAGDMYEDLAKIIINKYNDQFKDIKYTDNKILDDNVHSLFKQISIDYFLKIIKQQLADFNVKIKHWSSEQEVYDTHQIEKVLKLYESKDALYYKDDALFLKTTQFGDDKDRVLVKSDKTYTYILPDLATHNLRIKRTKADKLINVWGGDHHGYIKRMQAGLALLGNDPDILEIQMVQMVRLIKDGSEYKMSKRKGTAVWLVDILELVGVDALRYMLASKSSNSHMDLDLDLITLKNSSNPVYYAQYATARCHSILNQAKTKKITPLIKQTNLLNNPKEIELLLILDNFKEVIKNSANNRSTQQICDYIQNICKIFHSYYAEIKIIDENDLQLTKLRLGFIKAILQVLKNAFFIIGIQPVVEM"
     primer_bind     complement(90..106)
                     /note="common sequencing primer, one of multiple similar variants"
                     /label="M13 rev"
     rep_origin      complement(173..761)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(952..1812)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(1813..1917)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC
       61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT
      121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA
      181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC
      241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG
      301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC
      361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG
      421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC
      481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA
      541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG
      601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA
      661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG
      721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA
      781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA
      841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA
      901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG
      961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG
     1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC
     1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC
     1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA
     1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC
     1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG
     1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC
     1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT
     1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG
     1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC
     1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG
     1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT
     1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG
     1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG
     1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT
     1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC
     1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC
     1981 TTGGTTCAGG TGGAGTGGGA GGGTCTCAAA TGAGTACTAC AATCATCGAA ATGTTCTACA
     2041 ATGACTTGAA GAACATATGT CAGAAATTAA ACATTACTAA GGAACCGATT ATTGAAATCA
     2101 ACAAGAATAA CACCCCGGGG TTGCTTTCTT CCAGCATTTG TCTGATAAGT TCAAAACAGG
     2161 TAAACAAAAA ACCGTTAGAG TTGGCAGAAA TTTTTAAACA GGAACTTCTG CTGACGAACT
     2221 CGTATCTTAA CATCGACATT GCCGCTCCTG GATTCTTAAA CGTGTTGGTT AAACCCGAGA
     2281 TTCTGTCCAA TGTTATCTCG AACGTTTTAA GTCTTAAGAG CAAGTACGGT AACCTTGAGA
     2341 AACAAAACAA GACGATCAAC ATTGAGTATG TCTCGGCCAA TCCTACTGGC TATTTGCATG
     2401 TCGGTCATGC CAGAAACGCA GTGATCGGAT CCGTCCTTGT TAATCTTTTT AAAAAAGCGG
     2461 GATACAACGT ACAAACGGAA TACTACGTAA ATGACGCGGG AAACCAGATT AATGTATTGG
     2521 CCGTCACGGT GTTCGTACAT TATTTGCAAG CCTTAAACAT TGACGCTAAA AAACCAGAAA
     2581 ACTGTTACGC GGGCGACATG TATGAGGACT TAGCTAAAAT CATTATAAAC AAGTATAACG
     2641 ACCAGTTCAA GGATATTAAG TATACCGACA ATAAGATATT GGATGACAAC GTTCATAGTT
     2701 TGTTTAAGCA GATCTCCATT GACTACTTCT TGAAAATTAT AAAACAACAA TTGGCAGATT
     2761 TCAACGTTAA AATAAAACAT TGGTCGTCAG AACAAGAGGT CTACGACACA CACCAGATAG
     2821 AGAAAGTGTT AAAACTTTAC GAGTCAAAGG ATGCATTGTA CTACAAAGAC GATGCCCTGT
     2881 TCTTGAAAAC TACACAGTTC GGCGACGACA AAGATCGCGT CTTAGTCAAA TCCGATAAGA
     2941 CATATACGTA CATTCTGCCC GACTTGGCGA CTCATAATTT AAGAATAAAA AGAACGAAGG
     3001 CTGACAAACT TATTAATGTC TGGGGCGGTG ATCATCACGG CTACATCAAA CGGATGCAGG
     3061 CAGGGTTAGC ACTGCTGGGA AATGACCCAG ATATCCTTGA AATTCAAATG GTTCAGATGG
     3121 TGCGCCTTAT AAAGGACGGT AGCGAGTATA AGATGAGTAA GCGGAAAGGC ACGGCGGTTT
     3181 GGTTAGTAGA CATCCTTGAA TTAGTGGGGG TCGACGCACT GAGATACATG TTAGCGAGTA
     3241 AGAGTTCTAA TTCCCACATG GATTTGGATC TTGATCTTAT TACGCTGAAG AACTCTTCCA
     3301 ATCCCGTCTA TTACGCTCAG TATGCGACGG CGAGATGTCA CTCTATACTG AACCAGGCAA
     3361 AGACCAAGAA AATTACTCCT TTGATCAAGC AGACTAATTT GTTAAATAAC CCTAAGGAGA
     3421 TCGAACTTTT GTTAATATTG GACAACTTTA AGGAAGTCAT CAAGAACTCT GCCAACAACC
     3481 GGTCAACGCA ACAAATATGC GATTACATTC AAAACATATG TAAAATCTTC CACTCGTACT
     3541 ACGCGGAAAT CAAGATTATT GACGAGAACG ACTTACAATT GACAAAACTT AGACTGGGCT
     3601 TTATCAAAGC GATTCTTCAG GTCTTAAAGA ATGCGTTCTT TATAATAGGC ATCCAACCAG
     3661 TAGTAGAAAT GTGAAGAGCT TAGAGACC
//