LOCUS Exported 2945 bp DNA circular SYN 24-JUL-2020 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2001) AUTHORS Keoni Gandall TITLE Direct Submission JOURNAL Exported from Poly. FEATURES Location/Qualifiers terminator 1..2945 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" terminator 958..1005 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" primer_bind 2833..2849 /label="M13 for" /note="common sequencing primer, one of multiple similar variants" terminator 2850..2881 /label="tonB terminator" /note="bidirectional E. coli tonB-P14 transcription terminator" CDS 1..0 /note="tsf: translation elongation factor Ts" /translation="MAVDAKLIKELREITQAGMMDCKKALEASDNNIDNAIVWLRENGLAKAAKKTDRVAAEGIVLAKENDQKIVILEVNSETDFVAKNEKFLSLVDEIANALLNSNASSLEEGLQVKTNSGLTIEQSLISATATIGEKIALRRFELVNKTEGSSVIYNHANKRVSTLLVFDNKLDSTDAYNIAMHVAAMAPKYINMDQIPEDFKNAEMHIIKEQAKDDAKLQAKPANVLENILKGKLSKRLAEVSLLDQLFVIDESFKVGDFLKSKHVSLVKMIRYEVGEGIEKVVTNFADEVAAQLK" /label="MMSYN1_0539" primer_bind complement(1010..1026) /label="M13 rev" /note="common sequencing primer, one of multiple similar variants" rep_origin complement(1093..1681) /label="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(1852..2712) /label="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*" promoter complement(2713..2817) /label="bla promoter" /note="ampicillin resistance gene promoter" CDS complement(1..0) /label="MMSYN1_0539" /note="tsf: translation elongation factor Ts" /translation="MAVDAKLIKELREITQAGMMDCKKALEASDNNIDNAIVWLRENGLAKAAKKTDRVAAEGIVLAKENDQKIVILEVNSETDFVAKNEKFLSLVDEIANALLNSNASSLEEGLQVKTNSGLTIEQSLISATATIGEKIALRRFELVNKTEGSSVIYNHANKRVSTLLVFDNKLDSTDAYNIAMHVAAMAPKYINMDQIPEDFKNAEMHIIKEQAKDDAKLQAKPANVLENILKGKLSKRLAEVSLLDQLFVIDESFKVGDFLKSKHVSLVKMIRYEVGEGIEKVVTNFADEVAAQLK" ORIGIN 1 ATGGCAGTTG ATGCCAAGCT GATTAAGGAG CTTCGCGAGA TTACTCAAGC CGGTATGATG 61 GATTGTAAGA AGGCCCTGGA GGCGTCGGAC AATAATATTG ACAATGCCAT TGTGTGGCTT 121 CGTGAGAATG GGTTGGCGAA GGCGGCTAAA AAGACGGACC GTGTAGCTGC GGAAGGAATT 181 GTACTGGCTA AGGAGAACGA TCAGAAGATT GTGATTTTAG AGGTAAATTC TGAAACGGAT 241 TTTGTAGCGA AGAACGAGAA ATTTTTGTCT CTGGTAGATG AGATCGCTAA TGCGCTTCTG 301 AATAGTAATG CATCGAGTCT TGAAGAGGGT CTGCAAGTGA AAACAAACAG CGGTTTGACG 361 ATTGAGCAAT CTCTGATTTC TGCAACTGCG ACAATCGGAG AAAAAATCGC GTTACGCCGT 421 TTCGAACTTG TTAATAAAAC TGAGGGTTCT TCTGTCATTT ACAATCATGC GAATAAACGC 481 GTATCGACGT TATTGGTTTT TGATAACAAA CTGGACTCCA CAGACGCCTA CAACATTGCT 541 ATGCACGTAG CGGCAATGGC GCCGAAATAT ATCAACATGG ATCAAATTCC AGAGGACTTT 601 AAAAATGCTG AGATGCATAT CATTAAGGAG CAGGCTAAGG ACGATGCTAA ATTACAAGCC 661 AAACCCGCTA ATGTGTTGGA GAATATTTTA AAGGGTAAGC TGTCAAAACG TCTGGCCGAA 721 GTCTCTTTAT TGGACCAGCT TTTTGTAATC GATGAAAGTT TTAAGGTTGG AGACTTCCTT 781 AAATCCAAAC ATGTGTCGCT GGTTAAAATG ATCCGCTACG AAGTCGGTGA GGGTATTGAA 841 AAGGTTGTCA CCAACTTTGC AGATGAAGTA GCCGCGCAAT TAAAGTGAAG AGCTTAGAGA 901 CCGGTACTAG TTCCTGCAGG GCTCGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA 961 GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT 1021 TTCCTGAGAG CTTGGCAGGT GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA 1081 GAATGCCATT AATTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC 1141 AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG 1201 CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT 1261 CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA 1321 GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC 1381 CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC 1441 AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT 1501 GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT 1561 GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC 1621 TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA 1681 AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA 1741 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA 1801 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG 1861 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG 1921 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC 1981 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC 2041 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA 2101 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC 2161 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG 2221 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC 2281 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT 2341 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG 2401 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC 2461 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG 2521 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT 2581 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG 2641 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG 2701 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT 2761 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC 2821 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC 2881 TTGGTCAGGT GGAGTGAATT CGCGGCCGCT TCTAGAGGCT CTTCGTAATT ACGAAGAGGT 2941 CTCTA //