LOCUS       Exported     2945 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..2945
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      958..1005
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     2833..2849
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      2850..2881
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             1..0
                     /note="tsf: translation elongation factor Ts"
                     /translation="MAVDAKLIKELREITQAGMMDCKKALEASDNNIDNAIVWLRENGLAKAAKKTDRVAAEGIVLAKENDQKIVILEVNSETDFVAKNEKFLSLVDEIANALLNSNASSLEEGLQVKTNSGLTIEQSLISATATIGEKIALRRFELVNKTEGSSVIYNHANKRVSTLLVFDNKLDSTDAYNIAMHVAAMAPKYINMDQIPEDFKNAEMHIIKEQAKDDAKLQAKPANVLENILKGKLSKRLAEVSLLDQLFVIDESFKVGDFLKSKHVSLVKMIRYEVGEGIEKVVTNFADEVAAQLK"
                     /label="MMSYN1_0539"
     primer_bind     complement(1010..1026)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1093..1681)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(1852..2712)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(2713..2817)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
     CDS             complement(1..0)
                     /label="MMSYN1_0539"
                     /note="tsf: translation elongation factor Ts"
                     /translation="MAVDAKLIKELREITQAGMMDCKKALEASDNNIDNAIVWLRENGLAKAAKKTDRVAAEGIVLAKENDQKIVILEVNSETDFVAKNEKFLSLVDEIANALLNSNASSLEEGLQVKTNSGLTIEQSLISATATIGEKIALRRFELVNKTEGSSVIYNHANKRVSTLLVFDNKLDSTDAYNIAMHVAAMAPKYINMDQIPEDFKNAEMHIIKEQAKDDAKLQAKPANVLENILKGKLSKRLAEVSLLDQLFVIDESFKVGDFLKSKHVSLVKMIRYEVGEGIEKVVTNFADEVAAQLK"
ORIGIN
        1 ATGGCAGTTG ATGCCAAGCT GATTAAGGAG CTTCGCGAGA TTACTCAAGC CGGTATGATG
       61 GATTGTAAGA AGGCCCTGGA GGCGTCGGAC AATAATATTG ACAATGCCAT TGTGTGGCTT
      121 CGTGAGAATG GGTTGGCGAA GGCGGCTAAA AAGACGGACC GTGTAGCTGC GGAAGGAATT
      181 GTACTGGCTA AGGAGAACGA TCAGAAGATT GTGATTTTAG AGGTAAATTC TGAAACGGAT
      241 TTTGTAGCGA AGAACGAGAA ATTTTTGTCT CTGGTAGATG AGATCGCTAA TGCGCTTCTG
      301 AATAGTAATG CATCGAGTCT TGAAGAGGGT CTGCAAGTGA AAACAAACAG CGGTTTGACG
      361 ATTGAGCAAT CTCTGATTTC TGCAACTGCG ACAATCGGAG AAAAAATCGC GTTACGCCGT
      421 TTCGAACTTG TTAATAAAAC TGAGGGTTCT TCTGTCATTT ACAATCATGC GAATAAACGC
      481 GTATCGACGT TATTGGTTTT TGATAACAAA CTGGACTCCA CAGACGCCTA CAACATTGCT
      541 ATGCACGTAG CGGCAATGGC GCCGAAATAT ATCAACATGG ATCAAATTCC AGAGGACTTT
      601 AAAAATGCTG AGATGCATAT CATTAAGGAG CAGGCTAAGG ACGATGCTAA ATTACAAGCC
      661 AAACCCGCTA ATGTGTTGGA GAATATTTTA AAGGGTAAGC TGTCAAAACG TCTGGCCGAA
      721 GTCTCTTTAT TGGACCAGCT TTTTGTAATC GATGAAAGTT TTAAGGTTGG AGACTTCCTT
      781 AAATCCAAAC ATGTGTCGCT GGTTAAAATG ATCCGCTACG AAGTCGGTGA GGGTATTGAA
      841 AAGGTTGTCA CCAACTTTGC AGATGAAGTA GCCGCGCAAT TAAAGTGAAG AGCTTAGAGA
      901 CCGGTACTAG TTCCTGCAGG GCTCGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA
      961 GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT
     1021 TTCCTGAGAG CTTGGCAGGT GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA
     1081 GAATGCCATT AATTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC
     1141 AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG
     1201 CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT
     1261 CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA
     1321 GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC
     1381 CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC
     1441 AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT
     1501 GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT
     1561 GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC
     1621 TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA
     1681 AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA
     1741 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA
     1801 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG
     1861 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG
     1921 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC
     1981 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC
     2041 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA
     2101 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC
     2161 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG
     2221 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC
     2281 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT
     2341 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG
     2401 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC
     2461 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG
     2521 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT
     2581 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG
     2641 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG
     2701 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT
     2761 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC
     2821 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC
     2881 TTGGTCAGGT GGAGTGAATT CGCGGCCGCT TCTAGAGGCT CTTCGTAATT ACGAAGAGGT
     2941 CTCTA
//