LOCUS       Exported     2936 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..2936
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      949..996
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     2824..2840
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      2841..2872
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
                     /label="tonB terminator"
     CDS             1..0
                     /label="MMSYN1_0540"
                     /note=""
                     /translation="MSREITREELSAAGVQYGHQTKRWNPKMKSYIYGVKNKNHIIDLEKTITHLNTAQKLLETLGSKQQKILFVGTKRSGKNAVKEAALRSGNFYINNRWLGGTLTNLKTILIRIKALWEIEEEEKKGRLSLRTKKEQIKILKEKAKLEKALGGIKQMHKLPAAIVVVDPKGDEIAVKEAKKLNIPVIAICDTNADPDMIDYVIPGNDDLQESVNLIINILVEAYAEGAQIKMNPSVLKTVAPKREPRQNRVMTPVVENQSTEQQASENVSNTQVSNEPVTPVVEVEKSSEPKAE"
     primer_bind     complement(1001..1017)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1084..1672)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(1843..2703)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(2704..2808)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
     CDS             complement(1..0)
                     /label="MMSYN1_0540"
                     /note=""
                     /translation="MSREITREELSAAGVQYGHQTKRWNPKMKSYIYGVKNKNHIIDLEKTITHLNTAQKLLETLGSKQQKILFVGTKRSGKNAVKEAALRSGNFYINNRWLGGTLTNLKTILIRIKALWEIEEEEKKGRLSLRTKKEQIKILKEKAKLEKALGGIKQMHKLPAAIVVVDPKGDEIAVKEAKKLNIPVIAICDTNADPDMIDYVIPGNDDLQESVNLIINILVEAYAEGAQIKMNPSVLKTVAPKREPRQNRVMTPVVENQSTEQQASENVSNTQVSNEPVTPVVEVEKSSEPKAE"
ORIGIN
        1 ATGTCACGCG AGATCACGCG CGAAGAACTT AGCGCTGCGG GTGTACAGTA TGGGCATCAG
       61 ACTAAACGCT GGAATCCAAA GATGAAGTCG TACATTTATG GCGTTAAAAA TAAGAATCAC
      121 ATTATCGACC TTGAAAAGAC CATTACGCAT CTTAACACTG CCCAGAAATT ATTAGAAACC
      181 CTTGGAAGTA AGCAGCAAAA GATCCTTTTC GTTGGAACGA AGCGTTCCGG TAAAAATGCC
      241 GTCAAAGAAG CCGCCTTACG CTCCGGGAAC TTCTATATTA ATAACCGCTG GCTTGGAGGC
      301 ACATTAACGA ATTTGAAAAC CATTTTAATT CGTATTAAAG CCTTGTGGGA AATTGAGGAA
      361 GAAGAGAAAA AGGGCCGCTT GTCTTTACGC ACTAAGAAAG AGCAGATTAA GATCTTGAAA
      421 GAAAAAGCAA AATTAGAGAA GGCACTTGGA GGCATTAAAC AAATGCATAA ATTGCCAGCC
      481 GCAATTGTAG TAGTCGATCC AAAGGGTGAT GAGATCGCCG TTAAGGAAGC GAAGAAATTA
      541 AATATTCCCG TAATTGCAAT TTGTGACACC AACGCCGATC CTGACATGAT TGACTACGTT
      601 ATCCCCGGTA ATGACGATTT GCAAGAGAGT GTGAATCTGA TTATCAATAT TCTGGTTGAG
      661 GCATACGCTG AAGGCGCCCA GATTAAGATG AATCCATCCG TATTAAAGAC TGTGGCTCCC
      721 AAACGTGAGC CTCGTCAGAA CCGTGTCATG ACGCCTGTGG TAGAGAATCA GTCAACGGAG
      781 CAACAAGCTT CCGAGAATGT CTCGAATACA CAAGTTAGCA ACGAGCCCGT GACGCCTGTA
      841 GTGGAGGTTG AGAAGAGTTC AGAACCGAAG GCAGAGTGAA GAGCTTAGAG ACCGGTACTA
      901 GTTCCTGCAG GGCTCGAGGT GTCAATCGTC GGAGCCGCTG AGCAATAACT AGCATAACCC
      961 CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCATG GTCATAGCTG TTTCCTGAGA
     1021 GCTTGGCAGG TGATGACACA CATTAACAAA TTTCGTGAGG AGTCTCCAGA AGAATGCCAT
     1081 TAATTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG
     1141 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT
     1201 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG
     1261 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG
     1321 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG
     1381 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC
     1441 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG
     1501 GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT
     1561 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG
     1621 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC
     1681 TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT
     1741 GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT
     1801 TAAATCAATC TAAAGTATAT ATGAGTAAAC TTGGTCTGAC AGTTACCAAT GCTTAATCAG
     1861 TGAGGCACCT ATCTCAGCGA TCTGTCTATT TCGTTCATCC ATAGTTGCCT GACTCCCCGT
     1921 CGTGTAGATA ACTACGATAC GGGAGGGCTT ACCATCTGGC CCCAGTGCTG CAATGATACC
     1981 GCGAGAACCA CGCTCACCGG CTCCAGATTT ATCAGCAATA AACCAGCCAG CCGGAAGGGC
     2041 CGAGCGCAGA AGTGGTCCTG CAACTTTATC CGCCTCCATC CAGTCTATTA ATTGTTGCCG
     2101 GGAAGCTAGA GTAAGTAGTT CGCCAGTTAA TAGTTTGCGC AACGTTGTTG CCATTGCTAC
     2161 AGGCATCGTG GTGTCACGCT CGTCGTTTGG TATGGCTTCA TTCAGCTCCG GTTCCCAACG
     2221 ATCAAGGCGA GTTACATGAT CCCCCATGTT GTGCAAAAAA GCGGTTAGCT CCTTCGGTCC
     2281 TCCGATCGTT GTCAGAAGTA AGTTGGCCGC AGTGTTATCA CTCATGGTTA TGGCAGCACT
     2341 GCATAATTCT CTTACTGTCA TGCCATCCGT AAGATGCTTT TCTGTGACTG GTGAGTACTC
     2401 AACCAAGTCA TTCTGAGAAT AGTGTATGCG GCGACCGAGT TGCTCTTGCC CGGCGTCAAT
     2461 ACGGGATAAT ACCGCGCCAC ATAGCAGAAC TTTAAAAGTG CTCATCATTG GAAAACGTTC
     2521 TTCGGGGCGA AAACTCTCAA GGATCTTACC GCTGTTGAGA TCCAGTTCGA TGTAACCCAC
     2581 TCGTGCACCC AACTGATCTT CAGCATCTTT TACTTTCACC AGCGTTTCTG GGTGAGCAAA
     2641 AACAGGAAGG CAAAATGCCG CAAAAAAGGG AATAAGGGCG ACACGGAAAT GTTGAATACT
     2701 CATACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG
     2761 ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CCTGCACCAG
     2821 TCAGTAAAAC GACGGCCAGT AGTCAAAAGC CTCCGACCGG AGGCTTTTGA CTTGGTCAGG
     2881 TGGAGTGAAT TCGCGGCCGC TTCTAGAGGC TCTTCGTAAT TACGAAGAGG TCTCTA
//