LOCUS Exported 3176 bp DNA circular SYN 24-JUL-2020 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2001) AUTHORS Keoni Gandall TITLE Direct Submission JOURNAL Exported from Poly. FEATURES Location/Qualifiers terminator 1..3176 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" terminator 1189..1236 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" primer_bind 3064..3080 /note="common sequencing primer, one of multiple similar variants" /label="M13 for" terminator 3081..3112 /label="tonB terminator" /note="bidirectional E. coli tonB-P14 transcription terminator" CDS 1..0 /label="MMSYN1_0541" /note="DnaJ: chaperone protein DnaJ" /translation="MKKKDYYEVLGVSKTASEQEIRQAYRKLAKQYHPDLNKSPDAHDKMVEINEAADVLLDKDKRKQYDQFGHNAFDGSSGFSSNFADFEDLFSNMGSSGFSSFTNIFSDFFGSNKSDYQRSTKGQSVSVDIYLTFKELLFGADKIIELDLLTNCSVCFGSGAESNSDISICNNCHGTGEVLIQKNMGFFQFQQSAKCNVCNGAGKIIKNKCKNCKGKGKYLERQKIEVNIPKGIRPNQQIKLSQKGHASINNGVNGDLIIDIYLKESKVFEIVNNNDILMTYNISYLDSILGNEIIIKTLDGDIKYKLPKSINSNEFIIINNKGLYKSINKDKRGDLIIKVNIVVPKNLNKKEKELIEQIYEQTSFNPENNIDQ" primer_bind complement(1241..1257) /label="M13 rev" /note="common sequencing primer, one of multiple similar variants" rep_origin complement(1324..1912) /label="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(2083..2943) /label="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*" promoter complement(2944..3048) /label="bla promoter" /note="ampicillin resistance gene promoter" CDS complement(1..0) /label="MMSYN1_0541" /note="DnaJ: chaperone protein DnaJ" /translation="MKKKDYYEVLGVSKTASEQEIRQAYRKLAKQYHPDLNKSPDAHDKMVEINEAADVLLDKDKRKQYDQFGHNAFDGSSGFSSNFADFEDLFSNMGSSGFSSFTNIFSDFFGSNKSDYQRSTKGQSVSVDIYLTFKELLFGADKIIELDLLTNCSVCFGSGAESNSDISICNNCHGTGEVLIQKNMGFFQFQQSAKCNVCNGAGKIIKNKCKNCKGKGKYLERQKIEVNIPKGIRPNQQIKLSQKGHASINNGVNGDLIIDIYLKESKVFEIVNNNDILMTYNISYLDSILGNEIIIKTLDGDIKYKLPKSINSNEFIIINNKGLYKSINKDKRGDLIIKVNIVVPKNLNKKEKELIEQIYEQTSFNPENNIDQ" ORIGIN 1 ATGAAGAAAA AGGATTACTA CGAGGTCTTG GGAGTTAGCA AGACAGCTTC AGAACAAGAG 61 ATCCGTCAAG CTTACCGTAA GCTTGCAAAG CAGTATCATC CGGACTTAAA CAAGTCCCCT 121 GACGCCCACG ACAAAATGGT CGAGATTAAC GAGGCCGCGG ATGTGTTATT GGATAAAGAT 181 AAACGCAAAC AATATGACCA GTTCGGTCAC AATGCATTTG ATGGGTCAAG CGGGTTCAGT 241 TCAAACTTCG CAGACTTCGA GGACCTGTTT TCTAACATGG GTTCGAGTGG TTTCTCGTCT 301 TTCACAAACA TCTTTTCGGA TTTCTTCGGA TCAAATAAGT CAGATTATCA ACGCAGCACG 361 AAGGGTCAAT CGGTAAGTGT AGACATCTAT CTTACCTTCA AGGAGTTGTT GTTTGGCGCA 421 GACAAAATTA TTGAGCTGGA CTTATTAACC AATTGCTCTG TATGCTTTGG GAGCGGAGCT 481 GAGAGCAATT CAGACATTAG CATCTGTAAT AACTGTCATG GTACGGGTGA AGTTTTGATC 541 CAGAAAAATA TGGGTTTCTT TCAATTCCAG CAATCTGCCA AATGCAATGT GTGTAACGGA 601 GCTGGGAAGA TCATCAAAAA TAAATGTAAG AACTGCAAAG GGAAGGGCAA ATATCTGGAG 661 CGTCAGAAGA TTGAGGTCAA CATCCCTAAA GGTATCCGTC CCAATCAACA AATCAAACTT 721 AGCCAGAAGG GGCATGCGTC GATTAACAAT GGTGTAAATG GAGACTTGAT CATTGACATT 781 TATCTTAAGG AATCGAAAGT TTTCGAAATT GTTAATAACA ACGACATCTT AATGACGTAC 841 AACATCTCAT ACCTGGATAG CATTCTGGGC AATGAGATCA TTATCAAAAC CCTTGACGGA 901 GACATCAAAT ATAAGTTGCC CAAATCTATC AACTCCAATG AATTCATTAT CATTAACAAT 961 AAAGGCTTAT ACAAAAGTAT CAATAAAGAC AAGCGTGGTG ACCTGATCAT CAAGGTAAAC 1021 ATCGTCGTAC CGAAAAATCT TAATAAGAAA GAGAAGGAGC TTATTGAGCA GATTTATGAA 1081 CAGACATCCT TTAACCCTGA GAACAACATC GACCAGTGAA GAGCTTAGAG ACCGGTACTA 1141 GTTCCTGCAG GGCTCGAGGT GTCAATCGTC GGAGCCGCTG AGCAATAACT AGCATAACCC 1201 CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCATG GTCATAGCTG TTTCCTGAGA 1261 GCTTGGCAGG TGATGACACA CATTAACAAA TTTCGTGAGG AGTCTCCAGA AGAATGCCAT 1321 TAATTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG 1381 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT 1441 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG 1501 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG 1561 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG 1621 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC 1681 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG 1741 GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT 1801 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG 1861 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC 1921 TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT 1981 GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT 2041 TAAATCAATC TAAAGTATAT ATGAGTAAAC TTGGTCTGAC AGTTACCAAT GCTTAATCAG 2101 TGAGGCACCT ATCTCAGCGA TCTGTCTATT TCGTTCATCC ATAGTTGCCT GACTCCCCGT 2161 CGTGTAGATA ACTACGATAC GGGAGGGCTT ACCATCTGGC CCCAGTGCTG CAATGATACC 2221 GCGAGAACCA CGCTCACCGG CTCCAGATTT ATCAGCAATA AACCAGCCAG CCGGAAGGGC 2281 CGAGCGCAGA AGTGGTCCTG CAACTTTATC CGCCTCCATC CAGTCTATTA ATTGTTGCCG 2341 GGAAGCTAGA GTAAGTAGTT CGCCAGTTAA TAGTTTGCGC AACGTTGTTG CCATTGCTAC 2401 AGGCATCGTG GTGTCACGCT CGTCGTTTGG TATGGCTTCA TTCAGCTCCG GTTCCCAACG 2461 ATCAAGGCGA GTTACATGAT CCCCCATGTT GTGCAAAAAA GCGGTTAGCT CCTTCGGTCC 2521 TCCGATCGTT GTCAGAAGTA AGTTGGCCGC AGTGTTATCA CTCATGGTTA TGGCAGCACT 2581 GCATAATTCT CTTACTGTCA TGCCATCCGT AAGATGCTTT TCTGTGACTG GTGAGTACTC 2641 AACCAAGTCA TTCTGAGAAT AGTGTATGCG GCGACCGAGT TGCTCTTGCC CGGCGTCAAT 2701 ACGGGATAAT ACCGCGCCAC ATAGCAGAAC TTTAAAAGTG CTCATCATTG GAAAACGTTC 2761 TTCGGGGCGA AAACTCTCAA GGATCTTACC GCTGTTGAGA TCCAGTTCGA TGTAACCCAC 2821 TCGTGCACCC AACTGATCTT CAGCATCTTT TACTTTCACC AGCGTTTCTG GGTGAGCAAA 2881 AACAGGAAGG CAAAATGCCG CAAAAAAGGG AATAAGGGCG ACACGGAAAT GTTGAATACT 2941 CATACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG 3001 ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CCTGCACCAG 3061 TCAGTAAAAC GACGGCCAGT AGTCAAAAGC CTCCGACCGG AGGCTTTTGA CTTGGTCAGG 3121 TGGAGTGAAT TCGCGGCCGC TTCTAGAGGC TCTTCGTAAT TACGAAGAGG TCTCTA //