LOCUS       Exported     3799 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3799
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
                     /label="T7 terminator"
     terminator      38..85
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     1933..1949
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      1950..1981
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             2010..3785
                     /label="MMSYN1_0542"
                     /note="DnaK: chaperone protein DnaK"
                     /translation="MAKEKIIGIDLGTTNSVVSVIEGGQPIILENPEGQRTTPSVVAFKNSDIIVGGAAKRQAVTNPNVVQSIKSKMGTTSKVNLEGKDYSPEQISAEILRYMKNYAEAKLGQKVTKAVITVPAYFNDAQRKATKDAGTIAGLQVERIINEPTAAALAYGLDKQDKEETILVYDLGGGTFDVSILAIGGGSFDVIATSGNNKLGGDNFDEEIIKWLLGKIKAEYNIDLSKEKMALQRLKDEAEKAKINLSSQLEVEINLPFIAMNESGPISFATTLTRSEFNKITKHLVDLTIQPVKDALSAAKKTPSEINEVLLVGGSTRIPAVQELVKSLLNKEPNRSINPDEVVAMGAAVQGGVLAGEVTDILLLDVTPLSLGIETMGGVMTKLIERNTTIPAKRTQIFSTATDNQPAVDINVLQGERAMAADNKSLGQFQLTGIQPAPRGIPQIEVTFEIDANGIVSVSAKDKNTNEEKTITISNSGNLSEAEVERMIKEAQENAANDEVKKKNIELKNKAENYINIIETSLLQAGDKISAEQKEQSQKMVDEIKELVKNENYEALEQKMAELEQAMAQAAEFANKQNESDPNNNSSEQNN"
     primer_bind     complement(90..106)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(173..761)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(952..1812)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(1813..1917)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC
       61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT
      121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA
      181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC
      241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG
      301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC
      361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG
      421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC
      481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA
      541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG
      601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA
      661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG
      721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA
      781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA
      841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA
      901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG
      961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG
     1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC
     1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC
     1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA
     1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC
     1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG
     1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC
     1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT
     1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG
     1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC
     1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG
     1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT
     1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG
     1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG
     1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT
     1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC
     1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC
     1981 TTGGTTCAGG TGGAGTGGGA GGGTCTCAAA TGGCAAAGGA AAAAATTATC GGAATTGATT
     2041 TAGGGACGAC AAATTCCGTA GTAAGTGTCA TCGAGGGAGG CCAGCCCATC ATTCTTGAAA
     2101 ATCCCGAGGG ACAACGTACC ACGCCATCAG TCGTAGCGTT TAAGAACAGC GACATCATCG
     2161 TTGGTGGCGC CGCAAAGCGT CAGGCTGTTA CTAACCCCAA TGTAGTTCAG AGTATTAAAT
     2221 CTAAGATGGG CACCACATCT AAAGTGAACC TTGAAGGAAA AGACTATTCT CCGGAACAAA
     2281 TCTCTGCCGA AATCTTACGC TATATGAAAA ATTATGCAGA AGCGAAGCTT GGGCAGAAGG
     2341 TGACTAAAGC CGTCATTACT GTCCCCGCCT ACTTTAATGA TGCACAACGC AAAGCCACTA
     2401 AGGATGCAGG AACTATTGCA GGCCTTCAGG TTGAGCGTAT TATCAATGAG CCCACTGCAG
     2461 CTGCATTGGC GTACGGACTT GACAAGCAGG ACAAGGAGGA AACGATCCTT GTTTACGATT
     2521 TGGGTGGTGG AACATTCGAC GTTTCGATCT TAGCCATTGG AGGGGGGTCC TTCGATGTGA
     2581 TTGCGACGTC CGGAAATAAC AAGCTTGGGG GCGATAACTT TGACGAAGAA ATCATTAAGT
     2641 GGTTGTTAGG CAAGATTAAA GCTGAATATA ATATTGATTT GTCTAAGGAG AAAATGGCCC
     2701 TTCAACGCTT AAAGGATGAA GCTGAGAAAG CTAAGATTAA CTTATCCAGT CAGCTGGAAG
     2761 TGGAAATCAA TCTTCCATTC ATTGCAATGA ACGAGTCCGG GCCCATCAGC TTCGCCACAA
     2821 CATTGACCCG TTCAGAATTT AACAAAATCA CCAAGCATTT AGTGGACCTG ACAATTCAAC
     2881 CTGTAAAAGA CGCTCTGAGC GCCGCCAAAA AGACTCCCTC GGAGATCAAT GAGGTGCTTT
     2941 TGGTCGGGGG GAGCACACGC ATTCCAGCTG TACAGGAGTT AGTTAAATCG TTACTTAACA
     3001 AGGAGCCAAA CCGCTCAATC AACCCCGACG AGGTGGTCGC AATGGGGGCT GCCGTCCAAG
     3061 GGGGAGTATT GGCCGGAGAA GTCACAGACA TTTTGCTGCT TGACGTGACA CCCCTTTCTC
     3121 TGGGCATCGA AACAATGGGA GGCGTAATGA CCAAGTTGAT CGAGCGCAAT ACCACTATTC
     3181 CTGCGAAACG CACCCAGATT TTCAGTACGG CTACCGACAA TCAACCAGCG GTAGACATCA
     3241 ATGTGTTGCA GGGTGAGCGT GCAATGGCAG CCGACAACAA AAGTCTGGGG CAGTTCCAAT
     3301 TAACAGGTAT CCAACCGGCG CCCCGCGGCA TTCCCCAGAT CGAAGTGACG TTTGAAATCG
     3361 ATGCGAACGG CATTGTGTCC GTTTCAGCAA AGGACAAAAA TACGAATGAG GAAAAGACGA
     3421 TTACCATCTC GAACAGTGGT AACCTTTCGG AGGCTGAAGT GGAACGCATG ATTAAGGAAG
     3481 CGCAAGAGAA TGCCGCAAAT GATGAAGTTA AGAAGAAAAA TATTGAACTG AAAAATAAGG
     3541 CAGAAAACTA TATCAATATC ATCGAAACCA GTCTGTTACA GGCGGGTGAT AAAATCAGTG
     3601 CAGAGCAGAA AGAGCAGTCG CAGAAGATGG TGGATGAAAT TAAGGAGTTA GTTAAGAACG
     3661 AGAATTACGA AGCTTTGGAA CAGAAGATGG CTGAATTAGA ACAAGCAATG GCGCAAGCAG
     3721 CAGAATTCGC AAATAAACAA AACGAATCGG ATCCCAACAA TAATTCTAGC GAGCAGAACA
     3781 ACTGAAGAGC TTAGAGACC
//