LOCUS       Exported     3080 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3080
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1093..1140
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     2968..2984
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      2985..3016
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             1..0
                     /label="MMSYN1_0544"
                     /note="Negative regulator of class I heat shock genes (grpE-dnaK-dnaJ and groELS operons). Prevents heat-shock induction of these operons."
                     /translation="MLTNRQIKILQTIVEEFIKTNQPVGSKRILELLNMKISSATIRNESATLEHEGYLEKQHTSSGRTPSTKGYRYYVDNIMKLDSADYTRLKIYLNQLLDLRKYDIDKTINYASEIISELTKMTAVVIKKQNIKDIKLKKIELILLSEFLASVLFIFSDGDVQNKMFNLKDVALSDLKIAIKLFSDVLVDVKLDEIDQYLNDLKHQLSLSIKQYDYVLNTFINTILESKNEQKETHGMRYMLENPEFNDTNKLKNAVKLVEQLSPFDWFNIAYESNKNMNKIAIKIGNEIDQINDDISMIATELKIGNSSTVLTLVGPKRVDYNQVNQLMNLIIEIINTKEN"
     primer_bind     complement(1145..1161)
                     /note="common sequencing primer, one of multiple similar variants"
                     /label="M13 rev"
     rep_origin      complement(1228..1816)
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
                     /label="ori"
     CDS             complement(1987..2847)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(2848..2952)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
     CDS             complement(1..0)
                     /label="MMSYN1_0544"
                     /note="Negative regulator of class I heat shock genes (grpE-dnaK-dnaJ and groELS operons). Prevents heat-shock induction of these operons."
                     /translation="MLTNRQIKILQTIVEEFIKTNQPVGSKRILELLNMKISSATIRNESATLEHEGYLEKQHTSSGRTPSTKGYRYYVDNIMKLDSADYTRLKIYLNQLLDLRKYDIDKTINYASEIISELTKMTAVVIKKQNIKDIKLKKIELILLSEFLASVLFIFSDGDVQNKMFNLKDVALSDLKIAIKLFSDVLVDVKLDEIDQYLNDLKHQLSLSIKQYDYVLNTFINTILESKNEQKETHGMRYMLENPEFNDTNKLKNAVKLVEQLSPFDWFNIAYESNKNMNKIAIKIGNEIDQINDDISMIATELKIGNSSTVLTLVGPKRVDYNQVNQLMNLIIEIINTKEN"
ORIGIN
        1 ATGCTTACTA ACCGCCAGAT TAAAATTTTA CAAACTATCG TCGAGGAATT TATTAAAACG
       61 AACCAGCCCG TGGGGTCAAA GCGTATCCTT GAGTTGTTGA ATATGAAAAT CAGCTCTGCC
      121 ACCATCCGTA ACGAGTCAGC AACCTTAGAA CATGAAGGCT ACCTTGAAAA GCAGCATACT
      181 TCTTCAGGAC GCACGCCGTC GACAAAAGGT TACCGCTATT ACGTGGACAA CATCATGAAG
      241 CTTGACTCGG CAGACTACAC TCGTCTTAAA ATTTATTTGA ATCAACTTCT GGACCTTCGC
      301 AAATACGACA TCGATAAGAC AATTAACTAT GCTTCGGAGA TCATCAGCGA ACTTACGAAG
      361 ATGACGGCTG TGGTTATCAA GAAACAGAAC ATCAAGGACA TTAAACTGAA GAAAATTGAG
      421 TTGATTTTAT TGTCGGAGTT TCTTGCGTCA GTCCTGTTTA TTTTCTCCGA CGGTGACGTC
      481 CAGAATAAAA TGTTCAACTT GAAGGATGTA GCCCTTAGTG ACCTTAAAAT CGCGATCAAA
      541 TTATTCTCGG ACGTATTGGT TGATGTGAAG CTGGATGAGA TCGATCAGTA TTTAAACGAT
      601 CTGAAGCACC AGCTGTCCTT AAGTATTAAG CAGTACGACT ATGTTTTGAA CACGTTTATC
      661 AATACGATTC TTGAGAGCAA AAATGAACAA AAGGAAACAC ACGGTATGCG CTACATGCTT
      721 GAAAATCCCG AATTTAACGA TACGAATAAG TTGAAGAACG CGGTGAAGTT AGTAGAGCAG
      781 CTTAGTCCTT TTGACTGGTT CAATATCGCA TATGAGTCCA ACAAGAACAT GAATAAAATT
      841 GCTATCAAAA TTGGCAACGA AATCGACCAA ATTAACGATG ATATTAGTAT GATCGCAACT
      901 GAACTGAAAA TCGGTAACTC CTCCACGGTA TTAACGTTAG TGGGCCCCAA ACGCGTTGAC
      961 TACAACCAAG TTAACCAGTT AATGAACTTA ATTATCGAAA TCATTAATAC GAAAGAGAAC
     1021 TGAAGAGCTT AGAGACCGGT ACTAGTTCCT GCAGGGCTCG AGGTGTCAAT CGTCGGAGCC
     1081 GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG
     1141 CATGGTCATA GCTGTTTCCT GAGAGCTTGG CAGGTGATGA CACACATTAA CAAATTTCGT
     1201 GAGGAGTCTC CAGAAGAATG CCATTAATTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA
     1261 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC
     1321 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA
     1381 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA
     1441 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA
     1501 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA
     1561 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG
     1621 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG
     1681 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG
     1741 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG
     1801 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA
     1861 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT
     1921 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AAACTTGGTC
     1981 TGACAGTTAC CAATGCTTAA TCAGTGAGGC ACCTATCTCA GCGATCTGTC TATTTCGTTC
     2041 ATCCATAGTT GCCTGACTCC CCGTCGTGTA GATAACTACG ATACGGGAGG GCTTACCATC
     2101 TGGCCCCAGT GCTGCAATGA TACCGCGAGA ACCACGCTCA CCGGCTCCAG ATTTATCAGC
     2161 AATAAACCAG CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT CCTGCAACTT TATCCGCCTC
     2221 CATCCAGTCT ATTAATTGTT GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG TTAATAGTTT
     2281 GCGCAACGTT GTTGCCATTG CTACAGGCAT CGTGGTGTCA CGCTCGTCGT TTGGTATGGC
     2341 TTCATTCAGC TCCGGTTCCC AACGATCAAG GCGAGTTACA TGATCCCCCA TGTTGTGCAA
     2401 AAAAGCGGTT AGCTCCTTCG GTCCTCCGAT CGTTGTCAGA AGTAAGTTGG CCGCAGTGTT
     2461 ATCACTCATG GTTATGGCAG CACTGCATAA TTCTCTTACT GTCATGCCAT CCGTAAGATG
     2521 CTTTTCTGTG ACTGGTGAGT ACTCAACCAA GTCATTCTGA GAATAGTGTA TGCGGCGACC
     2581 GAGTTGCTCT TGCCCGGCGT CAATACGGGA TAATACCGCG CCACATAGCA GAACTTTAAA
     2641 AGTGCTCATC ATTGGAAAAC GTTCTTCGGG GCGAAAACTC TCAAGGATCT TACCGCTGTT
     2701 GAGATCCAGT TCGATGTAAC CCACTCGTGC ACCCAACTGA TCTTCAGCAT CTTTTACTTT
     2761 CACCAGCGTT TCTGGGTGAG CAAAAACAGG AAGGCAAAAT GCCGCAAAAA AGGGAATAAG
     2821 GGCGACACGG AAATGTTGAA TACTCATACT CTTCCTTTTT CAATATTATT GAAGCATTTA
     2881 TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT
     2941 AGGGGTTCCG CGCACCTGCA CCAGTCAGTA AAACGACGGC CAGTAGTCAA AAGCCTCCGA
     3001 CCGGAGGCTT TTGACTTGGT CAGGTGGAGT GAATTCGCGG CCGCTTCTAG AGGCTCTTCG
     3061 TAATTACGAA GAGGTCTCTA
//