LOCUS       Exported     4199 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..4199
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      2212..2259
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     4087..4103
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      4104..4135
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             1..0
                     /note="ClpB: chaperone protein ClpB"
                     /translation="MEFQEKGKVTDALKKYTRDLTKDAKDNKLDPVIGREEEISRVIQILSRKTKNNPVLIGEPGVGKTAIVEGLAQRIVKGDVPTLLKNKRILELDMGSLMAGAMYMGDYESRVKAVVNEIQKSNGEIILFIDELHLIVGAGKTGNNSGMDVSNLLKPALARGELKAIGSTTLNEYRQYIEKDAALERRFQRVLVSEPTIDQTISILRGLKDRFETYHGVRIHDNALVSAAKLSSRYITDRYLPDKAIDLVDEACASIRTELASVPIELDQVNRKVMQLEIETSALEKEKDDKSKERWQEAKKELDSLKIEQASLNEKWEKEKEELSRINSVKSSIESLKQELETAQNDGNYKRAGEIQYSLLPSLEKSLALFEKQTGSKMISEEVTEHEIAKVVSKSTGILVDRLISSEKEKLLNLEDLLKKYVKGQDQAIKAVTSAIMRSRSGIKNPDKPIGSFLFLGPTGVGKTEVARSLADILFNSPKKMIRLDMSEYMEKHSVAKLIGAPPGYVGYEEGGRLTEAVRRNPYSIVLFDEIEKAHTDVFNILLQILDDGRLTDSLGKTIDFKNTIIVMTSNIASQYLLTSDELVQVDDQKIQEELNKVFRPEFLNRIDNIVYFNALSVQTIGEIVDKVLEELSTRLQDEQNYFINFSEEARNKIINEGYDRLFGARPIKRYIEKNIETLIAHYIISGEVVENTRYLIDVKNNQFTLEEFKQFN"
                     /label="MMSYN1_0545"
     primer_bind     complement(2264..2280)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(2347..2935)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(3106..3966)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(3967..4071)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
     CDS             complement(1..0)
                     /translation="MEFQEKGKVTDALKKYTRDLTKDAKDNKLDPVIGREEEISRVIQILSRKTKNNPVLIGEPGVGKTAIVEGLAQRIVKGDVPTLLKNKRILELDMGSLMAGAMYMGDYESRVKAVVNEIQKSNGEIILFIDELHLIVGAGKTGNNSGMDVSNLLKPALARGELKAIGSTTLNEYRQYIEKDAALERRFQRVLVSEPTIDQTISILRGLKDRFETYHGVRIHDNALVSAAKLSSRYITDRYLPDKAIDLVDEACASIRTELASVPIELDQVNRKVMQLEIETSALEKEKDDKSKERWQEAKKELDSLKIEQASLNEKWEKEKEELSRINSVKSSIESLKQELETAQNDGNYKRAGEIQYSLLPSLEKSLALFEKQTGSKMISEEVTEHEIAKVVSKSTGILVDRLISSEKEKLLNLEDLLKKYVKGQDQAIKAVTSAIMRSRSGIKNPDKPIGSFLFLGPTGVGKTEVARSLADILFNSPKKMIRLDMSEYMEKHSVAKLIGAPPGYVGYEEGGRLTEAVRRNPYSIVLFDEIEKAHTDVFNILLQILDDGRLTDSLGKTIDFKNTIIVMTSNIASQYLLTSDELVQVDDQKIQEELNKVFRPEFLNRIDNIVYFNALSVQTIGEIVDKVLEELSTRLQDEQNYFINFSEEARNKIINEGYDRLFGARPIKRYIEKNIETLIAHYIISGEVVENTRYLIDVKNNQFTLEEFKQFN"
                     /label="MMSYN1_0545"
                     /note="ClpB: chaperone protein ClpB"
ORIGIN
        1 ATGGAGTTCC AAGAAAAAGG AAAAGTCACC GATGCTCTGA AGAAATACAC CCGCGATTTA
       61 ACCAAGGATG CGAAGGACAA TAAGCTGGAC CCAGTAATTG GGCGTGAAGA GGAAATCTCA
      121 CGCGTCATCC AGATTTTAAG CCGTAAAACC AAAAACAATC CCGTTTTAAT CGGAGAACCT
      181 GGTGTCGGCA AGACGGCCAT TGTTGAGGGC CTTGCTCAGC GTATCGTTAA GGGGGATGTT
      241 CCCACATTGC TGAAGAACAA ACGTATTTTG GAACTTGACA TGGGGTCTTT GATGGCTGGA
      301 GCTATGTATA TGGGCGATTA TGAGTCCCGC GTTAAGGCAG TGGTTAATGA AATTCAGAAA
      361 TCCAATGGCG AAATCATCCT GTTCATCGAT GAGCTTCATT TAATTGTGGG GGCCGGGAAA
      421 ACCGGAAATA ACAGCGGAAT GGACGTGTCA AATTTATTGA AGCCCGCGTT GGCTCGTGGC
      481 GAATTGAAGG CAATTGGATC CACGACTTTA AATGAGTATC GCCAATATAT TGAGAAAGAT
      541 GCAGCCTTGG AACGTCGCTT TCAGCGTGTT TTGGTGTCTG AGCCGACTAT CGACCAAACT
      601 ATCAGCATTC TTCGTGGGCT GAAAGACCGC TTCGAGACTT ATCACGGAGT ACGTATTCAC
      661 GATAATGCAT TAGTCAGTGC TGCCAAGTTA TCTAGCCGTT ACATTACGGA TCGTTACTTG
      721 CCGGACAAGG CGATTGACTT GGTTGATGAA GCATGCGCTT CGATTCGCAC GGAGCTGGCG
      781 TCCGTCCCAA TTGAGCTTGA CCAAGTAAAC CGCAAAGTGA TGCAATTAGA GATCGAGACA
      841 TCCGCATTAG AAAAGGAGAA AGACGATAAA AGCAAGGAGC GCTGGCAAGA AGCCAAAAAG
      901 GAATTGGATT CCCTGAAAAT TGAACAAGCG AGCTTGAATG AAAAATGGGA AAAAGAGAAA
      961 GAAGAACTTA GTCGCATCAA TAGTGTCAAA AGCAGTATTG AAAGTTTAAA ACAGGAACTG
     1021 GAGACAGCGC AGAACGATGG GAACTATAAG CGCGCGGGAG AAATTCAGTA CTCTCTTTTA
     1081 CCCTCACTTG AGAAGAGTTT AGCCTTGTTC GAGAAGCAAA CGGGTAGTAA AATGATTAGT
     1141 GAGGAAGTCA CAGAGCACGA GATTGCGAAG GTCGTTTCAA AGTCGACGGG CATTCTGGTC
     1201 GATCGCCTTA TTTCTTCGGA AAAAGAGAAG CTTCTGAATT TAGAGGACCT TCTTAAGAAA
     1261 TACGTGAAAG GCCAGGATCA GGCTATTAAA GCCGTCACGA GTGCAATTAT GCGCAGCCGC
     1321 TCTGGAATTA AAAATCCTGA CAAACCTATT GGTAGCTTCC TGTTCCTTGG CCCAACCGGT
     1381 GTAGGTAAAA CGGAAGTTGC TCGTTCTCTG GCAGACATCT TATTTAACTC TCCCAAAAAG
     1441 ATGATTCGCT TGGACATGAG CGAATACATG GAAAAACATT CCGTCGCCAA ACTTATTGGC
     1501 GCACCGCCTG GTTACGTAGG TTATGAGGAG GGGGGCCGCT TAACTGAGGC TGTTCGTCGT
     1561 AACCCATATT CCATCGTGTT ATTCGACGAG ATCGAGAAGG CCCATACTGA CGTGTTCAAT
     1621 ATTCTTTTGC AGATCCTGGA TGACGGCCGC TTGACCGATT CACTTGGCAA GACGATTGAC
     1681 TTTAAAAACA CAATTATTGT TATGACCTCG AATATCGCAT CTCAATACCT TCTGACCTCT
     1741 GACGAGCTTG TCCAGGTCGA CGATCAGAAA ATCCAGGAGG AATTAAATAA AGTATTTCGT
     1801 CCCGAATTCC TTAATCGCAT TGACAATATT GTGTACTTTA ATGCTCTTAG TGTACAGACG
     1861 ATCGGCGAGA TTGTCGATAA GGTTCTGGAA GAACTGAGCA CGCGCTTGCA AGACGAACAG
     1921 AACTACTTCA TTAATTTCAG CGAAGAAGCA CGCAACAAAA TCATTAATGA GGGATATGAT
     1981 CGCCTGTTTG GTGCGCGTCC CATTAAGCGC TATATCGAGA AAAACATCGA AACTCTGATC
     2041 GCGCATTACA TCATCTCTGG GGAAGTTGTT GAAAACACTC GTTACCTGAT CGATGTAAAA
     2101 AATAACCAAT TTACGTTGGA GGAATTTAAA CAGTTCAACT GAAGAGCTTA GAGACCGGTA
     2161 CTAGTTCCTG CAGGGCTCGA GGTGTCAATC GTCGGAGCCG CTGAGCAATA ACTAGCATAA
     2221 CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC ATGGTCATAG CTGTTTCCTG
     2281 AGAGCTTGGC AGGTGATGAC ACACATTAAC AAATTTCGTG AGGAGTCTCC AGAAGAATGC
     2341 CATTAATTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA
     2401 GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT
     2461 CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC
     2521 GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT
     2581 TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC
     2641 CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC
     2701 CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG
     2761 GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC
     2821 AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG
     2881 CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA
     2941 TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT
     3001 TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG
     3061 TTTTAAATCA ATCTAAAGTA TATATGAGTA AACTTGGTCT GACAGTTACC AATGCTTAAT
     3121 CAGTGAGGCA CCTATCTCAG CGATCTGTCT ATTTCGTTCA TCCATAGTTG CCTGACTCCC
     3181 CGTCGTGTAG ATAACTACGA TACGGGAGGG CTTACCATCT GGCCCCAGTG CTGCAATGAT
     3241 ACCGCGAGAA CCACGCTCAC CGGCTCCAGA TTTATCAGCA ATAAACCAGC CAGCCGGAAG
     3301 GGCCGAGCGC AGAAGTGGTC CTGCAACTTT ATCCGCCTCC ATCCAGTCTA TTAATTGTTG
     3361 CCGGGAAGCT AGAGTAAGTA GTTCGCCAGT TAATAGTTTG CGCAACGTTG TTGCCATTGC
     3421 TACAGGCATC GTGGTGTCAC GCTCGTCGTT TGGTATGGCT TCATTCAGCT CCGGTTCCCA
     3481 ACGATCAAGG CGAGTTACAT GATCCCCCAT GTTGTGCAAA AAAGCGGTTA GCTCCTTCGG
     3541 TCCTCCGATC GTTGTCAGAA GTAAGTTGGC CGCAGTGTTA TCACTCATGG TTATGGCAGC
     3601 ACTGCATAAT TCTCTTACTG TCATGCCATC CGTAAGATGC TTTTCTGTGA CTGGTGAGTA
     3661 CTCAACCAAG TCATTCTGAG AATAGTGTAT GCGGCGACCG AGTTGCTCTT GCCCGGCGTC
     3721 AATACGGGAT AATACCGCGC CACATAGCAG AACTTTAAAA GTGCTCATCA TTGGAAAACG
     3781 TTCTTCGGGG CGAAAACTCT CAAGGATCTT ACCGCTGTTG AGATCCAGTT CGATGTAACC
     3841 CACTCGTGCA CCCAACTGAT CTTCAGCATC TTTTACTTTC ACCAGCGTTT CTGGGTGAGC
     3901 AAAAACAGGA AGGCAAAATG CCGCAAAAAA GGGAATAAGG GCGACACGGA AATGTTGAAT
     3961 ACTCATACTC TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG
     4021 CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACCTGCAC
     4081 CAGTCAGTAA AACGACGGCC AGTAGTCAAA AGCCTCCGAC CGGAGGCTTT TGACTTGGTC
     4141 AGGTGGAGTG AATTCGCGGC CGCTTCTAGA GGCTCTTCGT AATTACGAAG AGGTCTCTA
//