LOCUS       Exported     3002 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3002
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1015..1062
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
                     /label="T7 terminator"
     primer_bind     2890..2906
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      2907..2938
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             1..0
                     /label="MMSYN1_0601"
                     /note="membrane protein, putative"
                     /translation="MKNNNSSFFSSPRTQIKVFQWVGTIFAVIGMLISLYFLSKINPQQLDQPKQVLLSLGYATMGYMFWKTIISAVIILRFVKKSTDEELVANRYILASLSLNLGGFLTPWILTSLPNVTTQSTIKPKWFLSRSFAIITTIGSAIFLGILFWQLKIIGPNTNWFDQTKEWYWILLGFIIGNGVLLVVGLLAFILFFNKNSKERFEGNTFTSFLMKTIAVFYLVIVTVELILLMIYSILRLIGNILNTARRVLQADNMFIGVLYLLFGLLSTFFQIYYVIFLTIMISQTIKGIWRKDGVITIKVYDKIQDNKNKYDLR"
     primer_bind     complement(1067..1083)
                     /note="common sequencing primer, one of multiple similar variants"
                     /label="M13 rev"
     rep_origin      complement(1150..1738)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(1909..2769)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(2770..2874)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
     CDS             complement(1..0)
                     /note="membrane protein, putative"
                     /translation="MKNNNSSFFSSPRTQIKVFQWVGTIFAVIGMLISLYFLSKINPQQLDQPKQVLLSLGYATMGYMFWKTIISAVIILRFVKKSTDEELVANRYILASLSLNLGGFLTPWILTSLPNVTTQSTIKPKWFLSRSFAIITTIGSAIFLGILFWQLKIIGPNTNWFDQTKEWYWILLGFIIGNGVLLVVGLLAFILFFNKNSKERFEGNTFTSFLMKTIAVFYLVIVTVELILLMIYSILRLIGNILNTARRVLQADNMFIGVLYLLFGLLSTFFQIYYVIFLTIMISQTIKGIWRKDGVITIKVYDKIQDNKNKYDLR"
                     /label="MMSYN1_0601"
ORIGIN
        1 ATGAAGAACA ACAATAGCAG CTTCTTTTCA AGCCCGCGTA CGCAAATCAA AGTGTTCCAA
       61 TGGGTTGGAA CGATTTTCGC CGTGATTGGC ATGTTAATTT CCTTGTATTT CCTGTCTAAG
      121 ATCAACCCCC AGCAGTTAGA TCAGCCAAAA CAGGTCCTTT TGAGCCTTGG TTACGCTACG
      181 ATGGGATATA TGTTCTGGAA AACAATCATC AGCGCGGTTA TTATCTTGCG CTTTGTGAAA
      241 AAGTCCACGG ACGAGGAGTT AGTTGCTAAT CGTTATATTT TAGCTTCACT GAGTTTAAAT
      301 CTGGGCGGGT TTCTTACCCC TTGGATTTTA ACGTCGTTGC CAAATGTCAC GACGCAGTCG
      361 ACGATCAAGC CAAAATGGTT CCTTAGTCGC AGTTTCGCAA TTATTACGAC CATTGGCAGT
      421 GCCATCTTTT TAGGAATTTT GTTCTGGCAG CTGAAGATTA TTGGTCCTAA CACGAACTGG
      481 TTTGACCAAA CTAAGGAATG GTATTGGATT TTGCTGGGTT TCATTATTGG TAATGGGGTC
      541 TTATTAGTGG TTGGTCTTTT GGCATTTATC CTGTTCTTTA ATAAAAATAG CAAGGAGCGC
      601 TTCGAAGGGA ACACGTTTAC GTCATTTCTT ATGAAAACGA TTGCTGTGTT TTACTTAGTA
      661 ATCGTAACCG TGGAACTTAT CTTATTGATG ATTTACAGCA TCCTTCGCTT AATCGGGAAC
      721 ATTTTAAATA CGGCACGCCG CGTACTGCAG GCCGATAACA TGTTCATCGG CGTACTTTAT
      781 TTACTGTTCG GGTTGCTGTC AACTTTCTTC CAAATTTACT ATGTTATCTT TCTTACGATC
      841 ATGATCTCGC AGACAATCAA GGGTATTTGG CGTAAGGACG GTGTAATTAC GATCAAGGTT
      901 TACGACAAAA TTCAAGACAA TAAGAACAAG TACGATTTAC GCTGAAGAGC TTAGAGACCG
      961 GTACTAGTTC CTGCAGGGCT CGAGGTGTCA ATCGTCGGAG CCGCTGAGCA ATAACTAGCA
     1021 TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCATGGTCA TAGCTGTTTC
     1081 CTGAGAGCTT GGCAGGTGAT GACACACATT AACAAATTTC GTGAGGAGTC TCCAGAAGAA
     1141 TGCCATTAAT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG
     1201 TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC
     1261 CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC
     1321 TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT
     1381 CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT
     1441 ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC
     1501 AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA
     1561 GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA
     1621 GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG
     1681 TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA
     1741 AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG
     1801 GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG
     1861 AAGTTTTAAA TCAATCTAAA GTATATATGA GTAAACTTGG TCTGACAGTT ACCAATGCTT
     1921 AATCAGTGAG GCACCTATCT CAGCGATCTG TCTATTTCGT TCATCCATAG TTGCCTGACT
     1981 CCCCGTCGTG TAGATAACTA CGATACGGGA GGGCTTACCA TCTGGCCCCA GTGCTGCAAT
     2041 GATACCGCGA GAACCACGCT CACCGGCTCC AGATTTATCA GCAATAAACC AGCCAGCCGG
     2101 AAGGGCCGAG CGCAGAAGTG GTCCTGCAAC TTTATCCGCC TCCATCCAGT CTATTAATTG
     2161 TTGCCGGGAA GCTAGAGTAA GTAGTTCGCC AGTTAATAGT TTGCGCAACG TTGTTGCCAT
     2221 TGCTACAGGC ATCGTGGTGT CACGCTCGTC GTTTGGTATG GCTTCATTCA GCTCCGGTTC
     2281 CCAACGATCA AGGCGAGTTA CATGATCCCC CATGTTGTGC AAAAAAGCGG TTAGCTCCTT
     2341 CGGTCCTCCG ATCGTTGTCA GAAGTAAGTT GGCCGCAGTG TTATCACTCA TGGTTATGGC
     2401 AGCACTGCAT AATTCTCTTA CTGTCATGCC ATCCGTAAGA TGCTTTTCTG TGACTGGTGA
     2461 GTACTCAACC AAGTCATTCT GAGAATAGTG TATGCGGCGA CCGAGTTGCT CTTGCCCGGC
     2521 GTCAATACGG GATAATACCG CGCCACATAG CAGAACTTTA AAAGTGCTCA TCATTGGAAA
     2581 ACGTTCTTCG GGGCGAAAAC TCTCAAGGAT CTTACCGCTG TTGAGATCCA GTTCGATGTA
     2641 ACCCACTCGT GCACCCAACT GATCTTCAGC ATCTTTTACT TTCACCAGCG TTTCTGGGTG
     2701 AGCAAAAACA GGAAGGCAAA ATGCCGCAAA AAAGGGAATA AGGGCGACAC GGAAATGTTG
     2761 AATACTCATA CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT
     2821 GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACCTG
     2881 CACCAGTCAG TAAAACGACG GCCAGTAGTC AAAAGCCTCC GACCGGAGGC TTTTGACTTG
     2941 GTCAGGTGGA GTGAATTCGC GGCCGCTTCT AGAGGCTCTT CGTAATTACG AAGAGGTCTC
     3001 TA
//